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354 results on '"Fonville, Manoj"'

7. Dermacentor reticulatus – a tick on its way from glacial refugia to a panmictic Eurasian population

Author

Bilbija, Branka, Spitzweg, Cäcilia, Papoušek, Ivo, Fritz, Uwe, Földvári, Gábor, Mullett, Martin, Ihlow, Flora, Sprong, Hein, Civáňová Křížová, Kristína, Anisimov, Nikolay, Belova, Oxana A., Bonnet, Sarah I., Bychkova, Elizabeth, Czułowska, Aleksandra, Duscher, Georg G., Fonville, Manoj, Kahl, Olaf, Karbowiak, Grzegorz, Kholodilov, Ivan S., Kiewra, Dorota, Krčmar, Stjepan, Kumisbek, Gulzina, Livanova, Natalya, Majláth, Igor, Manfredi, Maria Teresa, Mihalca, Andrei D., Miró, Guadalupe, Moutailler, Sara, Nebogatkin, Igor V., Tomanović, Snežana, Vatansever, Zati, Yakovich, Marya, Zanzani, Sergio, and Široký, Pavel

Published
2023
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15. The prevalence of pathogens in ticks collected from humans in Belgium, 2021, versus 2017.

Author

Philippe, Camille, Geebelen, Laurence, Hermy, Marie R. G., Dufrasne, François E., Tersago, Katrien, Pellegrino, Alessandro, Fonville, Manoj, Sprong, Hein, Mori, Marcella, and Lernout, Tinne

Subjects
TICK-borne encephalitis viruses, CASTOR bean tick, BORRELIA burgdorferi, TICK-borne diseases, DERMACENTOR, RICKETTSIA, ANAPLASMA phagocytophilum
Abstract

Background: Ticks carry a variety of microorganisms, some of which are pathogenic to humans. The human risk of tick-borne diseases depends on, among others, the prevalence of pathogens in ticks biting humans. To follow-up on this prevalence over time, a Belgian study from 2017 was repeated in 2021. Methods: During the tick season 2021, citizens were invited to have ticks removed from their skin, send them and fill in a short questionnaire on an existing citizen science platform for the notification of tick bites (TekenNet). Ticks were morphologically identified to species and life stage level and screened using multiplex qPCR targeting, among others, Borrelia burgdorferi (sensu lato), Anaplasma phagocytophilum, Borrelia miyamotoi, Neoehrlichia mikurensis, Babesia spp., Rickettsia helvetica and tick-borne encephalitis virus (TBEV). The same methodology as in 2017 was used. Results: In 2021, the same tick species as in 2017 were identified in similar proportions; of 1094 ticks, 98.7% were Ixodes ricinus, 0.8% Ixodes hexagonus and 0.5% Dermacentor reticulatus. A total of 928 nymphs and adults could be screened for the presence of pathogens. Borrelia burgdorferi (s.l.) was detected in 9.9% (95% CI 8.2–12.0%), which is significantly lower than the prevalence of 13.9% (95% CI 12.2–15.7%) in 2017 (P = 0.004). The prevalences of A. phagocytophilum (4.7%; 95% CI 3.5–6.3%) and R. helvetica (13.3%; 95% CI 11.2–15.6%) in 2021 were significantly higher compared to 2017 (1.8%; 95% CI 1.3–2.7% and 6.8%; 95% CI 5.6–8.2% respectively) (P < 0.001 for both). For the other pathogens tested, no statistical differences compared to 2017 were found, with prevalences ranging between 1.5 and 2.9% in 2021. Rickettsia raoultii was again found in D. reticulatus ticks (n = 3/5 in 2021). Similar to 2017, no TBEV was detected in the ticks. Co-infections were found in 5.1% of ticks. When combining co-infection occurrence in 2017 and 2021, a positive correlation was observed between B. burgdorferi (s.l.) and N. mikurensis and B. burgdorferi (s.l.) and B. miyamotoi (P < 0.001 for both). Conclusions: Although the 2021 prevalences fell within expectations, differences were found compared to 2017. Further research to understand the explanations behind these differences is needed. [ABSTRACT FROM AUTHOR]

Published
2024
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17. T(r)icky Environments: Higher Prevalence of Tick-Borne Zoonotic Pathogens in Rodents from Natural Areas Compared with Urban Areas.

Author

de Cock, Marieke P., Baede, Valérie O., Esser, Helen J., Fonville, Manoj, de Vries, Ankje, de Boer, Willem F., Mehl, Calvin, Ulrich, Rainer G., Schares, Gereon, Hakze-van der Honing, Renate W., van der Poel, Wim H. M., Sprong, Hein, and Maas, Miriam

Subjects
URBAN land use, LYMPHOCYTIC choriomeningitis virus, MICE, METHICILLIN-resistant staphylococcus aureus, ARTHROPOD vectors
Abstract

Background: Urban areas are unique ecosystems with stark differences in species abundance and composition compared with natural ecosystems. These differences can affect pathogen transmission dynamics, thereby altering zoonotic pathogen prevalence and diversity. In this study, we screened small mammals from natural and urban areas in the Netherlands for up to 19 zoonotic pathogens, including viruses, bacteria, and protozoan parasites. Materials and Methods: In total, 578 small mammals were captured, including wood mice (Apodemus sylvaticus), bank voles (Myodes glareolus), yellow-necked mice (Apodemus flavicollis), house mice (Mus musculus), common voles (Microtus arvalis), and greater white-toothed shrews (Crocidura russula). We detected a wide variety of zoonotic pathogens in small mammals from both urban and natural areas. For a subset of these pathogens, in wood mice and bank voles, we then tested whether pathogen prevalence and diversity were associated with habitat type (i.e., natural versus urban), degree of greenness, and various host characteristics. Results: The prevalence of tick-borne zoonotic pathogens (Borrelia spp. and Neoehrlichia mikurensis) was significantly higher in wood mice from natural areas. In contrast, the prevalence of Bartonella spp. was higher in wood mice from urban areas, but this difference was not statistically significant. Pathogen diversity was higher in bank voles from natural habitats and increased with body weight for both rodent species, although this relationship depended on sex for bank voles. In addition, we detected methicillin-resistant Staphylococcus aureus, extended-spectrum beta-lactamase/AmpC-producing Escherichia coli, and lymphocytic choriomeningitis virus for the first time in rodents in the Netherlands. Discussion: The differences between natural and urban areas are likely related to differences in the abundance and diversity of arthropod vectors and vertebrate community composition. With increasing environmental encroachment and changes in urban land use (e.g., urban greening), it is important to better understand transmission dynamics of zoonotic pathogens in urban environments to reduce potential disease risks for public health. [ABSTRACT FROM AUTHOR]

Published
2024
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26. Sand lizards (Lacerta agilis) decrease nymphal infection prevalence for tick-borne pathogens Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum in a coastal dune ecosystem

Author

Köhler, Clara Florentine, Sprong, Hein, Fonville, Manoj, Esser, Helen, de Boer, Willem Frederik, van der Spek, Vincent, Spitzen-van der Sluijs, Annemarieke, Köhler, Clara Florentine, Sprong, Hein, Fonville, Manoj, Esser, Helen, de Boer, Willem Frederik, van der Spek, Vincent, and Spitzen-van der Sluijs, Annemarieke

Abstract

Understanding which factors determine tick-borne disease hazard can contribute to effective disease control. In Europe, the hazard of the pathogens Borrelia burgdorferi s.l. and Anaplasma phagocytophilum is determined by local tick densities (mainly Ixodes ricinus) and the reservoir competence of the host species community. Sand lizards (Lacerta agilis) are common hosts for larvae and nymphs of I. ricinus and non-competent reservoirs for both pathogens. Consequently, high relative abundance of L. agilis is hypothesized to be associated with lower infection prevalence in nymphs. Here, we aimed to test whether this effectively occurs in natural settings. We sampled different habitat types within a heterogenous dune landscape at the Dutch coast and estimated (1) L. agilis densities, (2) host community competence, (3) the density and infection prevalence of questing I. ricinus ticks and (4) the number and infection prevalence of ticks feeding on L. agilis. Captured L. agilis had high tick burdens and contributed substantially to feeding I. ricinus larvae in their natural habitat. B. burgdorferi s.l. and A. phagocytophilum were virtually absent from feeding larvae and nymphs. The nymphal infection prevalence of both pathogens in questing ticks was lower in habitat types where L. agilis was more abundant. Hence, L. agilis strongly reduced community competence. The density of questing nymphs was higher in habitat types with denser vegetation and also varied more between habitat types than infection prevalence. As a result, nymphal density had a stronger effect on the density of infected ticks than did nymphal infection prevalence. Synthesis and applications. Coastal dune habitats favourable for L. agilis have lower densities of questing nymphs and a lower human infection hazard. These results might be applicable to similar ecosystems where L. agilis is present. From a public health perspective, this underlines the importance of preserving early successional habitat, as enc

Published
2023

34. Prevalence and predictors of vector-borne pathogens in Dutch roe deer

Author

Dep Pathobiologie, FAH theoretische epidemiologie, dFAH AVR, VPDC pathologie, dPB I&I, Wijburg, Sara R, Fonville, Manoj, de Bruin, Arnout, van Rijn, Piet A, Montizaan, Margriet G E, van den Broek, Jan, Sprong, Hein, Rijks, Jolianne M, Dep Pathobiologie, FAH theoretische epidemiologie, dFAH AVR, VPDC pathologie, dPB I&I, Wijburg, Sara R, Fonville, Manoj, de Bruin, Arnout, van Rijn, Piet A, Montizaan, Margriet G E, van den Broek, Jan, Sprong, Hein, and Rijks, Jolianne M

Published
2022

35. Self-reported symptoms and health complaints associated with exposure to Ixodes ricinus-borne pathogens

Author

IRAS OH Epidemiology Microbial Agents, Azagi, Tal, Harms, Margriet, Swart, Arno, Fonville, Manoj, Hoornstra, Dieuwertje, Mughini-Gras, Lapo, Hovius, Joppe W, Sprong, Hein, van den Wijngaard, Cees, IRAS OH Epidemiology Microbial Agents, Azagi, Tal, Harms, Margriet, Swart, Arno, Fonville, Manoj, Hoornstra, Dieuwertje, Mughini-Gras, Lapo, Hovius, Joppe W, Sprong, Hein, and van den Wijngaard, Cees

Published
2022

36. Quantitative microbial population study reveals geographical differences in bacterial symbionts of Ixodes ricinus

Author

Krawczyk, Aleksandra Iwona, Röttjers, Lisa, Fonville, Manoj, Takumi, Katsuhisa, Takken, Willem, Faust, Karoline, Sprong, Hein, Krawczyk, Aleksandra Iwona, Röttjers, Lisa, Fonville, Manoj, Takumi, Katsuhisa, Takken, Willem, Faust, Karoline, and Sprong, Hein

Abstract

Ixodes ricinus ticks vector pathogens that cause serious health concerns. Like in other arthropods, the microbiome may affect tick biology, with consequences for pathogen transmission. Here, we explored the bacterial communities of I. ricinus across its developmental stages and six geographic locations by the 16S rRNA amplicon sequencing, combined with quantification of the bacterial load. Our results show that the microbiome of I. ricinus is highly variable, but changes gradually, and ticks originating from geographically close forest sites express similar bacterial communities. This suggests that geography-related factors affect the infection rates of vertically-transmitted symbionts in I. ricinus. Since some symbionts, such as R. helvetica can cause disease in humans, we propose that public health investigations consider geographical differences in its infection rates.

Published
2022

37. Screen the unforeseen: Microbiomeprofiling for detection of zoonotic pathogens in wild rats

Author

de Cock, Marieke, Fonville, Manoj, de Vries, Ankje, Bossers, Alex, van den Bogert, Bartholomeus, Hakze-van der Honing, Renate, Koets, Ad, Sprong, Hein, van der Poel, Wim, Maas, Miriam, de Cock, Marieke, Fonville, Manoj, de Vries, Ankje, Bossers, Alex, van den Bogert, Bartholomeus, Hakze-van der Honing, Renate, Koets, Ad, Sprong, Hein, van der Poel, Wim, and Maas, Miriam

Abstract

Wild rats can host various zoonotic pathogens. Detection of these pathogens is commonly performed using molecular techniques targeting one or a few specific pathogens. However, this specific way of surveillance could lead to (emerging) zoonotic pathogens staying unnoticed. This problem may be overcome by using broader microbiome-profiling techniques, which enable broad screening of a sample's bacterial or viral composition. In this study, we investigated if 16S rRNA gene amplicon sequencing would be a suitable tool for the detection of zoonotic bacteria in wild rats. Moreover, we used virome-enriched (VirCapSeq) sequencing to detect zoonotic viruses. DNA from kidney samples of 147 wild brown rats (Rattus norvegicus) and 42 black rats (Rattus rattus) was used for 16S rRNA gene amplicon sequencing of the V3–V4 hypervariable region. Blocking primers were developed to reduce the amplification of rat host DNA. The kidney bacterial composition was studied using alpha- and beta-diversity metrics and statistically assessed using PERMANOVA and SIMPER analyses. From the sequencing data, 14 potentially zoonotic bacterial genera were identified from which the presence of zoonotic Leptospira spp. and Bartonella tribocorum was confirmed by (q)PCR or Sanger sequencing. In addition, more than 65% of all samples were dominated (>50% reads) by one of three bacterial taxa: Streptococcus (n = 59), Mycoplasma (n = 39) and Leptospira (n = 25). These taxa also showed the highest contribution to the observed differences in beta diversity. VirCapSeq sequencing in rat liver samples detected the potentially zoonotic rat hepatitis E virus in three rats. Although 16S rRNA gene amplicon sequencing was limited in its capacity for species level identifications and can be more difficult to interpret due to the influence of contaminating sequences in these low microbial biomass samples, we believe it has potential to be a suitable pre-screening method in the future to get a better overview of potent

Published
2022

39. Additional file 2 of Tick microbial associations at the crossroad of horizontal and vertical transmission pathways

Author

Krawczyk, Aleksandra Iwona, Röttjers, Sam, Coimbra-Dores, Maria João, Heylen, Dieter, Fonville, Manoj, Takken, Willem, Faust, Karoline, and Sprong, Hein

Abstract

Additional file 2: Figure S1. Abundance of the most abundant taxa, separated by quartiles of absolute bacterial density (16S rRNA content in ng/µL). Figure S2. Principal coordinate analysis of Bray–Curtis dissimilarities for tick microbiomes compared to blanks.

Published
2022
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47. Hedgehogs and Squirrels as Hosts of Zoonotic Bartonella Species

Author

Majerová, Karolina, primary, Gutiérrez, Ricardo, additional, Fonville, Manoj, additional, Hönig, Václav, additional, Papežík, Petr, additional, Hofmannová, Lada, additional, Lesiczka, Paulina Maria, additional, Nachum-Biala, Yaarit, additional, Růžek, Daniel, additional, Sprong, Hein, additional, Harrus, Shimon, additional, Modrý, David, additional, and Votýpka, Jan, additional

Published
2021
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48. Additional file 1 of Occurrence of tick-borne pathogens in questing Ixodes ricinus ticks from Wester Ross, Northwest Scotland

Author

Olsthoorn, Fanny, Sprong, Hein, Fonville, Manoj, Rocchi, Mara, Medlock, Jolyon, Gilbert, Lucy, and Ghazoul, Jaboury

Abstract

Additional file 1: Text S1. Field methods explained. Table S1. Sum of ticks collected at each visit on each plot is summarised, as well as the sum of ticks positive for each of the eight pathogens tested for in the study, namely Borrelia miyamotoi, Borrelia burgdorferi s.l., Anaplasma phagocytophilum, Neoehrlichia mikurensis, Babesia spp. from clade X, Spiroplasma ixodetis, Babesia microti, and Rickettsia helvetica. Table S2. Molecular analysis results of the 2828 adult and nymph ticks.

Published
2021
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50. Circulation of Babesia Species and Their Exposure to Humans through Ixodes Ricinus

Author

LS Theoretische Epidemiologie, LS Infectiebiologie (Bacteriologie), VPDC pathologie, dPB CR, dPB I&I, Dep Pathobiologie, Sub GZ Herkauwer, Dep Biologie, Azagi, Tal, Jaarsma, Ryanne I, Docters van Leeuwen, Arieke, Fonville, Manoj, Maas, Miriam, Franssen, Frits F J, Kik, Marja, Rijks, Jolianne M, Montizaan, Margriet G, Groenevelt, Margit, Hoyer, Mark, Esser, Helen J, Krawczyk, Aleksandra I, Modrý, David, Sprong, Hein, Demir, Samiye, LS Theoretische Epidemiologie, LS Infectiebiologie (Bacteriologie), VPDC pathologie, dPB CR, dPB I&I, Dep Pathobiologie, Sub GZ Herkauwer, Dep Biologie, Azagi, Tal, Jaarsma, Ryanne I, Docters van Leeuwen, Arieke, Fonville, Manoj, Maas, Miriam, Franssen, Frits F J, Kik, Marja, Rijks, Jolianne M, Montizaan, Margriet G, Groenevelt, Margit, Hoyer, Mark, Esser, Helen J, Krawczyk, Aleksandra I, Modrý, David, Sprong, Hein, and Demir, Samiye

Published
2021

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