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Background: Periventricular nodular heterotopia (PVNH) is a neuronal migration disorder caused by the inability of neurons to move to the cortex. Patients with PVNH often experience epilepsy due to ectopic neuronal discharges. Most cases of PVNH are associated with variations in filamin A (FLNA), which encodes an actin-binding protein. However, the underlying pathological mechanisms remain unclear. Methods: Next-generation sequencing was performed to detect variants in the patient with PVNH, and the findings were confirmed using Sanger sequencing. Iterative threading assembly refinement was used to predict the structures of the variant proteins, and the search tool for the retrieval of interacting genes/proteins database was used to determine the interactions between FLNA and motility-related proteins. An induced pluripotent stem cell (iPSC) line was generated as a disease model by reprogramming human peripheral blood mononuclear cells. The FLNA expression in iPSCs was assessed using western blot and quantitative real-time polymerase chain reaction (qRT-PCR). Immunofluorescence analysis was performed to determine the arrangement of F-actin. Results: A novel FLNA frameshift variant (NM_001456.3: c.1466delG, p. G489Afs*9) was identified in a patient with PVNH and epilepsy. Bioinformatic analysis indicated that this variation was likely to impair FLNA function. Western blot and qRT-PCR analysis of iPSCs derived from the patient's peripheral blood mononuclear cells revealed the absence of FLNA protein and mRNA. Immunofluorescence analysis suggested an irregular arrangement and disorganization of F-actin compared to that observed in healthy donors. Conclusion: Our findings indicate that the frameshift variant of FLNA (NM_001456.3: c.1466delG, p. G489Afs*9) impairs the arrangement and organization of F-actin, potentially influencing cell migration and causing PVNH. [ABSTRACT FROM AUTHOR]

C63H63LaO27, trigonal, R3 (no. 146), a = 27.5427(11) Å, c = 7.7330(4) Å, V = 5080.3(5) Å3, Z = 3, R gt(F) = 0.037, wR ref(F 2) = 0.096, T = 100 K.

C23H25NO9, orthorhombic, P21212 (no. 18), a = 15.8641(6) Å, b = 11.1230(4) Å, c = 13.0925(5) Å, V = 2310.26(15) Å3, Z = 4, Rgt(F) = 0.0372, wRref(F2) = 0.0883, T = 298 K.

C8H8N4O3Zn, tetragonal, P4‾c2$P‾{4}c2$ (no. 116), a = 7.340(1) Å, c = 18.285(4) Å, V = 985.1(3) Å3, Z = 4, Rgt(F) = 0.0404, ωRref(F2) = 0.1214, T = 100 K.

In order to reduce pesticide application, develop green pest prevention and control technology, and ensure the quality and safety of agricultural products, a green pest prevention and control system was designed which composed of multi-functional plant protection machine and information management system, for facility vegetables based on the internet of things (IoT) technology, and combined with ozone sterilization and disease prevention, light source induced pest control, crop health identification, pest identification and early warning and other technologies. The results showed that under the green control system, the incidence rate of strawberry powdery mildew decreased by 27.7%, and the average yield of strawberry increased by 19.8% compared with the conventional application method, which had significant effect on powdery mildew control and potential of strawberry yield increase. Meanwhile, the functions of system early warning service, various disinfection and sterilization methods, remote control and intelligent trusteeship had effectively alleviated the problems of backward plant protection technology and equipment, excessive dependence on pesticide use, structural shortage of labor force, and maintenance and operation difficulties in China. The application of the system realized the greening, specialization and intellectualization of vegetable pest control in facilities to a certain extent. [ABSTRACT FROM AUTHOR]

Six novel lanthanide complexes ([Nd2(L)(H2O)6]n·4.58n(H2O) (1), [Ln(H3L)(H2O)]n·0.5n(H2O), Ln = Sm (2), Eu (3), Gd (4), Tb (5), Eu0.18Gd0.62Tb0.20 (6)) have been hydrothermally synthesized based on the ligand 4,5-di(3,5-dicarboxylphenoxy)phthalic acid (H6L). Single crystal X-ray diffraction reveals that complexes 1–6 are 2D structures, where 2–6 are isomorphic. Complexes 3 and 5 exhibit the characteristic fluorescence of Eu(III) and Tb(III) ions respectively, while complex 4 shows blue-green light emission based on the ligand. In particular, the ternary Eu/Gd/Tb complex 6 shows white light emission with a CIE (Commission International del'Eclairage) chromaticity coordinate of (0.330, 0.339) and hence close to pure white light emission. Moreover, complexes 3 and 5 display specific fluorescence-enhanced detection performance for Pb2+ ions: The interaction between Pb2+ ions and the ligand enhances the charge transfer efficiency between the ligand and the Eu(III) and Tb(III) ions and thus leads to fluorescence enhancement of complexes 3 and 5. More importantly, complex 3 exhibits the lowest detection limit of 4.72 nM for Pb2+ ions among the existing complex fluorescent probes. In addition, both complexes 3 and 5 show good performance for recycling and for the detection of Pb2+ in real water samples. [ABSTRACT FROM AUTHOR]

Herein, we report a novel 1/GO/Fe3O4 photocatalyst, comprising Ce(BTB)(H2O) (MOF-1, H3BTB = 1,3,5-benzenetrisbenzoic acid), graphene oxide (GO), and iron oxide (Fe3O4) for photocatalytic degradation of chlortetracycline (CTC). This design enables the effective transfer of electrons from the MOF to GO, thereby reducing the photoelectron–hole recombination rate. Therefore, the optimized 1/GO/Fe3O4 photocatalyst with H2O2 shows the highest photocatalytic activity toward CTC. The kinetic constant is 5.4 times that in the system of MOF-1 and hydrogen peroxide, which usually acted as efficient electron acceptors to improve the photocatalytic performance of MOFs. More importantly, light absorption is extended from the ultraviolet to the visible region. Furthermore, 1/GO/Fe3O4 can be quickly recycled under an applied magnetic field and displays outstanding stability and reusability. According to the radical trapping experiments and electron paramagnetic resonance results, hydroxyl radicals, superoxide radicals, and holes all contribute to excellent photocatalytic activity. The possible catalytic mechanism of 1/GO/Fe3O4 is tentatively proposed. This work aims to explore the synergistic effect between metal–organic frameworks (MOFs) and GO, and provide a theoretical basis for MOF-based composites to remove antibiotic contaminants in the environment. [ABSTRACT FROM AUTHOR]

C11H13CrN2O9, orthorhombic, Pbca (no. 61), a = 6.7740(14) Å, b = 12.309(3) Å, c = 32.776(7) Å, V = 2732.9(10) Å3, Z = 8, Rgt(F) = 0.0302, wRref(F2) = 0.0867, T = 100(2) K.

C4H8CoO8, monoclinic, P21/c (no. 14), a = 5.7952(3) Å, b = 13.0110(7) Å, c = 10.2682(6) Å, β = 98.8320(10)°, V = 765.06(7) Å3, Z = 4, Rgt(F) = 0.0207, ωRref(F2) = 0.0499, T = 301(2) K.

To protect ecosystem balance and human health, the development of fluorescent sensing materials with high sensitivity and portability has attracted wide attention in several decades. Herein, six lanthanide isostructural complexes {[Ln(μ6-Hcaa)(H2O)]Cl}n (H3caa = N-(4-carboxylbenzyl)- L -aspartic acid, Ln3+ = Ce3+ (1), Pr3+ (2), Nd3+ (3), Sm3+ (4), Eu3+ (5), and Tb3+ (6)) with optical properties based on aspartic acid derivative (H3caa) were synthesized by the solvothermal method and characterized in detail. It is worth noting that complex 6 can not only specifically recognize Cr(VI) with very low detection limits (LODs) of 3.66 nM (Cr2O72−) & 5.35 nM (CrO42−) but also selectively recognize TCs with LODs of 0.24 μM (CTC = chlortetracycline) and 0.25 μM (TC = tetracycline) based on the method of fluorescence detection. In addition, the identification of Cr(VI) and TCs by visual colorimetry may be realized through the combination of a smartphone and portable test strips. This study suggests that complex 6 is a good optical material for detecting heavy metals and antibiotic contaminants in aqueous systems and broadens the development of amino acid derivatives. [ABSTRACT FROM AUTHOR]