7 results on '"Fabio Francesco di Mola"'
Search Results
2. Laparoscopic Versus Open Hartmann Reversal: A Case-Control Study
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Paolo Panaccio, Tommaso Grottola, Rossana Percario, Federico Selvaggi, Severino Cericola, Alfonso Lapergola, Maira Farrukh, Giuseppe Di Martino, Marco Ricciardiello, Pierluigi Di Sebastiano, and Fabio Francesco Di Mola
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Surgery ,RD1-811 - Abstract
Background. Laparoscopic reversal of Hartmann’s procedure (LHR) offers reduced morbidity compared with open Hartmann’s reversal (OHR). The aim of this study is to compare the outcome of laparoscopic versus open Hartmann reversal. Materials and Methods. Thirty-four patients who underwent Hartmann reversal between January 2017 and July 2019 were evaluated. Patients underwent either LHR (n = 17) or OHR (n = 17). Variables such as numbers of patients, patient’s age, sex, body mass index (BMI), comorbidities, ASA (American Society of Anesthesiology) score, indication for previous open sigmoid resection, mean operation time, rate of conversion to open surgery, length of hospital stay, mortality, and morbidity were retrospectively evaluated. Results. The two groups of patients were homogeneous for gender, age, body mass index, cause of primary surgery, time to reversal, and comorbidities. In 97% of the cases, HP was done by open surgery. Our data revealed no difference in mean operation time (LHR: 180.5 ± 35.1 vs. OHR: 225.2 ± 48.4) and morbidity rate, although, in OHR group, there were more severe complications. Less intraoperative blood loss (LHR: 100 ± 40 mL vs. OHR: 450 ± 125 mL; p value
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- 2021
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3. Correlations among PPAR𝜸, DNMT1, and DNMT3B Expression Levels and Pancreatic Cancer
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Valerio Pazienza, Francesca Tavano, Giorgia Benegiamo, Manlio Vinciguerra, Francesca Paola Burbaci, Massimiliano Copetti, Fabio Francesco di Mola, Angelo Andriulli, and Pierluigi di Sebastiano
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Biology (General) ,QH301-705.5 - Abstract
Emerging evidence indicates that peroxisome proliferator-activated receptor γ (PPARγ) and DNA methyltransferases (DNMTs) play a role in carcinogenesis. In this study we aimed to evaluate the expression of PPARγ, DNMT1, and DNMT3B and their correlation with clinical-pathological features in patients with pancreatic cancer (PC), and to define the effect of PPARγ activation on DNMTs expression in PC cell lines. qRT-PCR analysis showed that DNMT3B expression was downregulated in tumors compared to normal tissues (𝑃=0.03), whereas PPARγ and DNMT1 levels did not show significant alterations in PC patients. Expression levels between PPARγ and DNMT1 and between DNMT1 and DNMT3B were highly correlated (𝑃=0.008 and 𝑃=0.05 resp.). DNMT3B overexpression in tumor tissue was positively correlated with both lymph nodes spreading (𝑃=0.046) and resection margin status (𝑃=0.04), and a borderline association with perineural invasion (𝑃=0.06) was found. Furthermore, high levels of DNMT3B expression were significantly associated with a lower mortality in the whole population (HR=0.485; 95%CI=0.262–0.895, 𝑃=0.02) and in the subgroup of patients without perineural invasion (HR=0.314; 95%CI=0.130–0.758; 𝑃=0.01), while such association was not observed in patients with tumor invasion into perineural structures (𝑃=0.70). In conclusion, in vitro and in vivo PPARγ and DNMTs appear interrelated in PC, and this interaction might influence cell phenotype and disease behavior.
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- 2012
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4. Time-Qualified Patterns of Variation of PPARγ, DNMT1, and DNMT3B Expression in Pancreatic Cancer Cell Lines
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Valerio Pazienza, Francesca Tavano, Massimo Francavilla, Andrea Fontana, Fabio Pellegrini, Giorgia Benegiamo, Vincenzo Corbo, Fabio Francesco di Mola, Pierluigi Di Sebastiano, Angelo Andriulli, and Gianluigi Mazzoccoli
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Biology (General) ,QH301-705.5 - Abstract
Carcinogenesis is related to the loss of homeostatic control of cellular processes regulated by transcriptional circuits and epigenetic mechanisms. Among these, the activities of peroxisome proliferator-activated receptors (PPARs) and DNA methyltransferases (DNMTs) are crucial and intertwined. PPARγ is a key regulator of cell fate, linking nutrient sensing to transcription processes, and its expression oscillates with circadian rhythmicity. Aim of our study was to assess the periodicity of PPARγ and DNMTs in pancreatic cancer (PC). We investigated the time-related patterns of PPARG, DNMT1, and DNMT3B expression monitoring their mRNA levels by qRT-PCR at different time points over a 28-hour span in BxPC-3, CFPAC-1, PANC-1, and MIAPaCa-2 PC cells after synchronization with serum shock. PPARG and DNMT1 expression in PANC-1 cells and PPARG expression in MIAPaCa-2 cells were characterized by a 24 h period oscillation, and a borderline significant rhythm was observed for the PPARG, DNMT1, and DNMT3B expression profiles in the other cell lines. The time-qualified profiles of gene expression showed different shapes and phase relationships in the PC cell lines examined. In conclusion, PPARG and DNMTs expression is characterized by different time-qualified patterns in cell lines derived from human PC, and this heterogeneity could influence cell phenotype and human disease behaviour.
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- 2012
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5. High Blood Concentration of Leukocyte-Derived Extracellular Vesicles Is Predictive of Favorable Clinical Outcomes in Patients with Pancreatic Cancer: Results from a Multicenter Prospective Study
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Davide Brocco, Domenico De Bellis, Pietro Di Marino, Pasquale Simeone, Antonino Grassadonia, Michele De Tursi, Tommaso Grottola, Fabio Francesco Di Mola, Patrizia Di Gregorio, Barbara Zappacosta, Antonio Angelone, Laura De Lellis, Serena Veschi, Rosalba Florio, Simone De Fabritiis, Fabio Verginelli, Marco Marchisio, Marta Caporale, Dimitri Luisi, Pierluigi Di Sebastiano, Nicola Tinari, Alessandro Cama, and Paola Lanuti
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Cancer Research ,pancreatic cancer ,leukocytes ,extracellular vesicles ,Oncology - Abstract
Pancreatic cancer (PC) is one of the leading causes of cancer-related death worldwide. Identification of novel tumor biomarkers is highly advocated in PC to optimize personalized treatment algorithms. Blood-circulating extracellular vesicles hold promise for liquid biopsy application in cancer. We used an optimized flow cytometry protocol to study leukocyte-derived EVs (CD45+) and PD-L1+ EVs in blood from 56 pancreatic cancer patients and 48 healthy controls (HCs). Our results show that PC patients presented higher blood levels of total EVs (p = 0.0003), leukocyte-derived EVs (LEVs) (p = 0.001) and PD-L1+ EVs (p = 0.01), as compared with HCs. Interestingly, a blood concentration of LEVs at baseline was independently associated with improved overall survival in patients with borderline resectable or primary unresectable PC (HR = 0.17; 95% CI 0.04–0.79; p = 0.02). Additionally, increased blood-based LEVs were independently correlated with prolonged progression-free survival (HR = 0.10; 95% CI 0.01–0.82; p = 0.03) and significantly associated with higher disease control rate (p = 0.02) in patients with advanced PC receiving standard chemotherapy. Notably, a strong correlation between a decrease in blood LEVs concentration during chemotherapy and disease control was observed (p = 0.005). These intriguing findings point to the potential of LEVs as novel blood-based EV biomarkers for improved personalized medicine in patients affected by PC.
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- 2022
6. Expression of the Antiapoptotic Protein BAG3 Is a Feature of Pancreatic Adenocarcinoma and Its Overexpression Is Associated With Poorer Survival. Turco MC: Corresponding Author
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Rosati, Alessandra, Samantha, Bersani, Francesca, Tavano, Elisa Dalla Pozza, DE MARCO, Margot, Marta, Palmieri, Vincenzo De Laurenzi, Renato, Franco, Giosuè, Scognamiglio, Raffaele, Palaia, Andrea, Fontana, Pierluigi di Sebastiano, Massimo, Donadelli, Ilaria, Dando, Jan Paul Medema, Frederike, Dijk, Lieke, Welling, Fabio Francesco di Mola, Raffaele, Pezzilli, Turco, Maria Caterina, and Aldo, Scarpa
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- 2012
7. Overexpressed decorin in pancreatic cancer: potential tumor growth inhibition and attenuation of chemotherapeutic action
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Jörg, Köninger, Nathalia A, Giese, Fabio Francesco, di Mola, Pascal, Berberat, Thomas, Giese, Irene, Esposito, Max G, Bachem, Markus W, Büchler, and Helmut, Friess
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Adult ,Male ,Antimetabolites, Antineoplastic ,Deoxycytidine ,Carboplatin ,Cell Line, Tumor ,Humans ,Drug Interactions ,RNA, Messenger ,Pancreas ,Cells, Cultured ,Aged ,Cell Proliferation ,Aged, 80 and over ,Extracellular Matrix Proteins ,Reverse Transcriptase Polymerase Chain Reaction ,Middle Aged ,Immunohistochemistry ,Gemcitabine ,Extracellular Matrix ,Up-Regulation ,Gene Expression Regulation, Neoplastic ,Pancreatic Neoplasms ,Female ,Proteoglycans ,Decorin - Abstract
The aim of this study was to investigate the expression and significance of decorin in pancreatic cancer.Decorin expression in normal pancreas and excised tumors was examined by real-time quantitative PCR, Western blot analysis, and immunohistochemistry. Reverse transcription-PCR was used to analyze cultures of pancreatic cancer and stellate cells. Growth-inhibitory effects of decorin in vitro were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test, Western blot, and fluorescence-activated cell-sorting analysis.Pancreatic cancer was characterized by striking overexpression of decorin mRNA in tumor tissues (9-fold by real-time quantitative PCR; 44 patients versus 18 healthy donors; P0.01). Strong decorin immunostaining was observed in the extracellular matrix of pancreatic cancer tissue, whereas tumor cells were devoid of decorin. Double staining for anti-smooth muscle actin and decorin and reverse transcription-PCR analysis of primary cultures revealed pancreatic stellate cells as the putative source of decorin. Human recombinant decorin was able to suppress growth of pancreatic cancer cells in vitro through p21 mediated G(1)-S block of the cell cycle. However, in contrast to the previously described chemotherapy-potentiating capacity of decorin, this proteoglycan attenuated the cytostatic action of carboplatin and gemcitabine toward pancreatic cancer cells.Decorin might exert an antiproliferative effect toward pancreatic cancer cells, thus playing a role in a host stromal reaction aimed at sequestering and inhibiting growing malignant cells. However, in clinical settings, the importance of collagen-associated decorin as a moderate antitumor modality would be undermined by its ability to attenuate the efficiency of chemotherapeutics. Considering the general failure of adjuvant therapies in pancreatic cancer, the role of decorin in this process warrants further investigation.
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- 2004
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