21 results on '"Eun Ah Jung"'
Search Results
2. In Vitro Probiotic Evaluation and Anti-Adipogenic Effect of Lactic Acid Bacteria Isolated from Kimchi
- Author
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Sung-Won Choi and Eun-Ah Jung
- Subjects
General Medicine - Published
- 2022
3. Prevalence of Mutations in Discoidin Domain-Containing Receptor Tyrosine Kinase 2 (DDR2) in Squamous Cell Lung Cancers in Korean Patients
- Author
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Eun Ah Jung, Sung Bin An, Yoon-La Choi, Joungho Han, Ji-Young Song, Doo-Yi Oh, Mi-Sook Lee, Yu Jin Kim, and Sang-Won Um
- Subjects
Male ,0301 basic medicine ,Cancer Research ,Pathology ,Lung Neoplasms ,medicine.disease_cause ,Receptor tyrosine kinase ,Mice ,0302 clinical medicine ,Gene Frequency ,Squamous cell carcinoma ,Prevalence ,Neoplasm Metastasis ,Aged, 80 and over ,Mutation ,biology ,Exons ,Middle Aged ,Immunohistochemistry ,Tumor Burden ,Dasatinib ,Oncology ,030220 oncology & carcinogenesis ,Carcinoma, Squamous Cell ,Female ,Original Article ,KRAS ,medicine.drug ,medicine.medical_specialty ,Cell Line ,03 medical and health sciences ,Discoidin Domain Receptor 2 ,Asian People ,Somatic mutations ,Republic of Korea ,medicine ,Animals ,Humans ,Lung cancer ,Alleles ,Aged ,Neoplasm Staging ,Cell growth ,business.industry ,Sequence Analysis, DNA ,medicine.disease ,030104 developmental biology ,Tumor progression ,Cancer research ,biology.protein ,Tomography, X-Ray Computed ,business ,Discoidin domain - Abstract
Purpose The discoidin domain-containing receptor tyrosine kinase 2 (DDR2) is known to contain mutations in a small subset of patients with squamous cell carcinomas (SCC) of the lung. Studying the DDR2 mutations in patients with SCC of the lung would advance our understanding and guide the development of therapeutic strategies against lung cancer. Materials and methods We selected 100 samples through a preliminary genetic screen, including specimens from biopsies and surgical resection, and confirmed SCC by histologic examination. DDR2 mutations on exons 6, 15, 16, and 18 were analyzed by Sanger sequencing of formalin-fixed, paraffin-embedded tissue samples. The functional effects of novel DDR2 mutants were confirmed by in vitro assays. Results We identified novel somatic mutations of DDR2 in two of the 100 SCC samples studied. One mutation was c.1745T>A (p.V582E) and the other was c.1784T>C (p.L595P), and both were on exon 15. Both patients were smokers and EGFR/KRAS/ALK-triple negative. The expression of the mutant DDR2 induced activation of DDR2 by the collagen ligand and caused enhanced cell growth and tumor progression. Moreover, dasatinib, a DDR2 inhibitor, showed potential efficacy against DDR2 L595P mutant-bearing cells. Conclusion Our results suggest that a mutation in DDR2 occurs naturally with a frequency of about 2% in Korean lung SCC patients. In addition, we showed that each of the novel DDR2 mutations were located in a kinase domain and induced an increase in cell proliferation rate.
- Published
- 2017
4. The c-Abl inhibitor, Radotinib HCl, is neuroprotective in a preclinical Parkinson's disease mouse model
- Author
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Eun Ah Jung, Wonjoong Richard Kim, Han Seok Ko, Jae Soo Shin, Dong-Hoon Kim, Hyun Soo Ju, Dae Jin Cho, Seung-Hwan Kwon, Jae Hong Park, Seung Pil Yun, Saebom Lee, Gong Yeal Lee, Hyo Jung Yun, Yong Joo Park, Seulki Lee, Sangjune Kim, and Dong Yeon Kim
- Subjects
0301 basic medicine ,Parkinson's disease ,Dopamine ,Biology ,Pharmacology ,Blood–brain barrier ,Neuroprotection ,03 medical and health sciences ,chemistry.chemical_compound ,Mice ,0302 clinical medicine ,hemic and lymphatic diseases ,Genetics ,medicine ,Animals ,Humans ,Proto-Oncogene Proteins c-abl ,Molecular Biology ,Genetics (clinical) ,Neuroinflammation ,Alpha-synuclein ,Dopaminergic Neurons ,Brain ,Parkinson Disease ,General Medicine ,medicine.disease ,Disease Models, Animal ,030104 developmental biology ,medicine.anatomical_structure ,Neuroprotective Agents ,Pyrimidines ,chemistry ,Nilotinib ,Blood-Brain Barrier ,Nerve Degeneration ,alpha-Synuclein ,Lewy Bodies ,Tyrosine kinase ,Sesquiterpenes ,030217 neurology & neurosurgery ,medicine.drug - Abstract
Accumulating evidence suggests that the non-receptor tyrosine kinase c-Abl plays an important role in the progression of Parkinson's disease (PD) and c-Abl inhibition could be neuroprotective in PD and related α-synucleinopathies. Nilotinib, a c-Abl inhibitor, has shown improved motor and cognitive symptoms in PD patients. However, issues concerning blood-brain barrier (BBB) penetration, lack of selectivity and safety still remain. Radotinib HCl is a selective Bcr-Abl kinase inhibitor that not only effectively access the brain, but also exhibits greater pharmacokinetic properties and safety profiles compared to Nilotinib and other c-Abl inhibitors. Here, we show the neuroprotective efficacy of Radotinib HCl, a brain penetrant c-Abl inhibitor, in a pre-clinical model of PD. Importantly, in vitro studies demonstrate that the treatment of Radotinib HCl protects the α-synuclein preformed fibrils (PFF)-induced neuronal toxicity, reduces the α-synuclein PFF-induced Lewy bodies (LB)/Lewy neurites (LN)-like pathology and inhibits the α-synuclein PFF-induced c-Abl activation in primary cortical neurons. Furthermore, administration of Radotinib HCl inhibits c-Abl activation and prevents dopaminergic neuron loss, neuroinflammation and behavioral deficits following α-synuclein PFF-induced toxicity in vivo. Taken together, our findings indicate that Radotinib HCl has beneficial neuroprotective effects in PD and provides an evidence that selective and brain permeable c-Abl inhibitors can be potential therapeutic agents for the treatment of PD and related α-synucleinopathies.
- Published
- 2018
5. Prevalence of the CTNNB1 mutation genotype in surgically resected fibromatosis of the breast
- Author
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Suk-Jin Nam, Ji Young Song, Jong Sun Choi, Jee Eun Kwon, So Young Kang, Jung Hee Shin, Yoon-La Choi, Taeeun Kim, Jung-Hyun Yang, Eun Yoon Cho, Ji Hyeon Roh, and Eun Ah Jung
- Subjects
Pathology ,medicine.medical_specialty ,Histology ,medicine.diagnostic_test ,business.industry ,Breast imaging ,Fibromatosis ,Soft tissue ,General Medicine ,medicine.disease ,Pathology and Forensic Medicine ,Biopsy ,Genotype ,medicine ,Carcinoma ,Mammography ,business ,Pathological - Abstract
Kim T, Jung E A, Song J Y, Roh J H, Choi J S, Kwon J E, Kang S Y, Cho E Y, Shin J H, Nam S-J, Yang J H & Choi Y-L (2012) Histopathology 60, 347–356 Prevalence of the CTNNB1 mutation genotype in surgically resected fibromatosis of the breast Aims: To investigate CTNNB1 mutation and β-catenin expression in resected breast fibromatosis and to identify potential molecular markers of fibromatosis of the breast. Methods and results: We selected 12 patients with fibromatosis of the breast who underwent surgical resection and were confirmed by histological examination. Ultrasonography findings for 10 patients were reviewed and only two cases were suspicious for fibromatosis on imaging. On core needle biopsy for pre-operative diagnoses, only three cases were histologically suspicious for fibromatosis. Mutations in exon 3 of CTNNB1 were detected by direct DNA sequencing in nine (75.0%) cases: all were c.121G>A (p.T41A), which was much more frequent in breast fibromatoses than in other soft tissue lesions. Nuclear β-catenin expression was observed in all cases and the level of expression was higher in cases with mutation. In eight of nine cases, the matched biopsy specimen showed the same CTNNB1 mutation status as the pre-operative specimen. Conclusions: In the majority of cases, clinical presentation and breast imaging are highly suspicious for carcinoma. Definitive pre-operative pathological diagnosis by core needle biopsy is difficult. CTNNB1 mutation and nuclear β-catenin expression are frequently detected in sporadic breast fibromatoses, suggesting their potential as a useful tool to distinguish breast fibromatoses from other neoplasms.
- Published
- 2011
6. Nodular Mass in the Upper Lip
- Author
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Eun Ah Jung, Junhui Jeong, and Jaesung Nam
- Subjects
Adult ,Male ,business.industry ,Adenoma, Pleomorphic ,Upper lip ,030206 dentistry ,Anatomy ,Salivary Gland Neoplasms ,Lip ,03 medical and health sciences ,0302 clinical medicine ,Otorhinolaryngology ,030220 oncology & carcinogenesis ,Humans ,Medicine ,Surgery ,business - Published
- 2018
7. Daio-Orengedokuto Inhibits HMG-CoA Reductase and Pancreatic Lipase
- Author
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Eun-Ah Jung, Sang-Kwan Moon, Nam-Jae Kim, Dong-Hyun Kim, Jong-Chul Chang, Ki-Ho Cho, Hyung-Kil Yang, Hyung-Sup Bae, Young-Suk Kim, and Ji-Eun Shin
- Subjects
Male ,medicine.medical_specialty ,Pharmaceutical Science ,Hyperlipidemias ,Reductase ,Rats, Sprague-Dawley ,Internal medicine ,Total cholesterol ,medicine ,Animals ,Pancreatic lipase ,Lipase ,Pancreas ,Lipoprotein cholesterol ,Pharmacology ,biology ,General Medicine ,Rats ,Serum triglyceride levels ,Endocrinology ,HMG-CoA reductase ,biology.protein ,Hydroxymethylglutaryl CoA Reductases ,Hydroxymethylglutaryl-CoA Reductase Inhibitors ,Drugs, Chinese Herbal - Abstract
To evaluate the antihyperlipidemic activities of Orengedokuto (OT) and Daio-Orengedokuto (DOT), the inhibitory effects of these polyprescriptions on HMG-CoA reductase and pancreatic lipase and on the rat hyperlipidemic model induced by Triton WR-1339 were measured. OT potently inhibited HMG-CoA reductase but did not inhibit lipase. Among their ingredients, Coptidis Rhizoma was the most potent inhibitor, followed by Rhei Rhizoma. The HMG-CoA reductase-inhibitory activity of 80% EtOH extract was superior to that of water extract. However, DOT potently inhibited HMG CoA-reductase as well as pancreatic lipase. In the rat hyperlipidemic model induced by Triton WR-1339, OT and DOT decreased serum total cholesterol and low-density lipoprotein cholesterol levels. DOT also decreased serum triglyceride levels, but OT did not decrease it. These results suggest that the antihyperlipidemic activity of DOT may originate from the inhibition of pancreatic lipase as well as HMG-CoA reductase.
- Published
- 2002
8. Characterization of aBacteroidesspecies from human intestine that degrades glycosaminoglycans
- Author
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Mi Young Ahn, Kuk Hyun Shin, Dong-Hyun Kim, Eun-Ah Jung, Toshihiko Toida, Robert J Linhardt, and Yeong Shik Kim
- Subjects
Immunology ,Genetics ,General Medicine ,Molecular Biology ,Applied Microbiology and Biotechnology ,Microbiology - Abstract
Polysaccharide lyases that can degrade glycosaminoglycans (GAGs) were identified in an anaerobic strain living in the human intestine. The strain was isolated from the stool of a healthy male and identified as Bacteroides sp. strain HJ-15. A detailed taxonomical study indicated the species is a strain of Bacteroides stercoris. The isolate was cultured and the polysaccharide lyase activity was partially purified. This enzyme preparation could act on GAGs containing either glucosamine or galactosamine suggesting the presence of both heparinases and chondroitinases. Various GAGs were incubated with the partially purified enzyme and the products formed were analyzed by strong anion-exchange high performance liquid chromatography and proton nuclear magnetic resonance spectroscopy. These studies demonstrated the presence of at least two types of polysaccharide lyases: heparin lyase and chondroitin sulfate lyase. The eliminative mechanism of these lyase enzymes was confirmed through the isolation of unsaturated disaccharide products. The heparin lyase acted on both heparin and acharan sulfate, a GAG recently isolated from Achatina fulica. The Bacteroides chondroitin lyase, acted on chondroitin sulfates A, B (dermatan sulfate), and C, resembling chondroitin lyase ABC. The presence of a GAG-degrading organism in human intestine may pose problems for the effective oral administration of GAG drugs.Key words: Bacteroides stercoris, glycosaminoglycan, nuclear magnetic resonance spectroscopy, polysaccharide lyase, heparinase, chondroitinase.
- Published
- 1998
9. Characterization of a Bacteroides species from human intestine that degrades glycosaminoglycans
- Author
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Dong-Hyun Kim, Mi Young Ahn, Kuk Hyun Shin, Toshihiko Toida, Yeong Shik Kim, Robert J. Linhardt, and Eun-Ah Jung
- Subjects
chemistry.chemical_classification ,biology ,Strain (chemistry) ,fungi ,Immunology ,food and beverages ,General Medicine ,biology.organism_classification ,Polysaccharide ,Applied Microbiology and Biotechnology ,Microbiology ,Heparin lyase ,Glycosaminoglycan ,chemistry.chemical_compound ,chemistry ,Biochemistry ,Genetics ,Molecular Biology ,Bacteroidaceae ,Bacteria ,Carbon-Oxygen Lyases ,Chondroitin AC lyase - Abstract
Polysaccharide lyases that can degrade glycosaminoglycans (GAGs) were identified in an anaerobic strain living in the human intestine. The strain was isolated from the stool of a healthy male and i...
- Published
- 1998
10. The c-Abl inhibitor, Radotinib HCl, is neuroprotective in a preclinical Parkinson's disease mouse model.
- Author
-
Saebom Lee, Sangjune Kim, Yong Joo Park, Seung Pil Yun, Seung-Hwan Kwon, Donghoon Kim, Dong Yeon Kim, Jae Soo Shin, Dae Jin Cho, Gong Yeal Lee, Hyun Soo Ju, Hyo Jung Yun, Jae Hong Park, Wonjoong Richard Kim, Eun Ah Jung, Seulki Lee, and Han Seok Ko
- Published
- 2018
- Full Text
- View/download PDF
11. Metabolism of glycyrrhizin and baicalin by human intestinal bacteria
- Author
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Kyeu-Yup Lee, Eun-Ah Jung, Hyeong Kyu Lee, Dong-Hyun Kim, and Il-Sung Jang
- Subjects
chemistry.chemical_compound ,Hydrolysis ,Biochemistry ,chemistry ,Organic Chemistry ,Drug Discovery ,Pharmacology toxicology ,Molecular Medicine ,Intestinal bacteria ,Metabolism ,Glycyrrhizin ,Baicalin ,Baicalein - Abstract
By human intestinal bacteria, glycyrrhizin (18β-glycyrrhetic acid β-D-glucuronyl α-D-glucuronic acid, GL) and baicalin (baicalein β-D-glucuronic acid) were metabolized to glycyrrhetinic acid and baicalin, respectively. However, α-glucuronidase ofBacteroides JY-6 isolated from human intestinal bacteria hydrolyzed GL or 18β-glycyrrhetinic acid α-D-glucuronic acid to 18β-glycyrrhetic acid but did not baicalin. However,E. coli β-glucuronidase from human intestinal bacteria hydrolyzed baicalin to baicalein, but did not GL. β-Glucuronidase of mammalian tissues hydrolyzed both GL and baicalin.
- Published
- 1996
12. Rapid Progression of Mediastinal Tumor within a Few Days: A Case Report of T Cell Lymphoblastic Lymphoma
- Author
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Young Kyung Lee, Tae Ran Ahn, Hyun Jung Jun, Jin Sung Son, and Eun Ah Jung
- Subjects
lcsh:Medical physics. Medical radiology. Nuclear medicine ,0301 basic medicine ,precursor t-cell lymphoblastic leukemia-lymphoma ,business.industry ,lcsh:R895-920 ,T cell ,Lymphoblastic lymphoma ,Mediastinal tumor ,medicine.disease ,Precursor T-Cell Lymphoblastic Leukemia-Lymphoma ,Mediastinal Neoplasm ,Non-Hodgkin's lymphoma ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,medicine.anatomical_structure ,hemic and lymphatic diseases ,030220 oncology & carcinogenesis ,Cancer research ,Medicine ,Radiology, Nuclear Medicine and imaging ,mediastinal neoplasms ,non-hodgkin's lymphoma ,business - Abstract
T-cell lymphoblastic lymphoma is a highly aggressive tumor derived from lymphocyte of the thymus, which accounts for 2% of non-Hodgkin's lymphoma. The disease occurs most commonly in adolescent and young adult males. It often results in respiratory emergency because of high proliferation rate. In this case, we confirmed the rapid progression of T-cell lymphoblastic lymphoma through the chest CT scan with one week interval. Three days of empirical chemotherapy resulted in substantial reduction of mediastinal mass, pleural thickening and pleural effusion.
- Published
- 2016
13. Prevalence of the CTNNB1 mutation genotype in surgically resected fibromatosis of the breast
- Author
-
Taeeun, Kim, Eun Ah, Jung, Ji Young, Song, Ji Hyeon, Roh, Jong Sun, Choi, Jee Eun, Kwon, So Young, Kang, Eun Yoon, Cho, Jung Hee, Shin, Suk-Jin, Nam, Jung Hyun, Yang, and Yoon-La, Choi
- Subjects
Adult ,Adolescent ,Genotype ,Breast Neoplasms ,Exons ,Fibroma ,Middle Aged ,Diagnosis, Differential ,Young Adult ,Fibroadenoma ,Phyllodes Tumor ,Mutation ,Biomarkers, Tumor ,Prevalence ,Humans ,Female ,Breast ,beta Catenin ,Aged ,Mammography ,Retrospective Studies - Abstract
To investigate CTNNB1 mutation and β-catenin expression in resected breast fibromatosis and to identify potential molecular markers of fibromatosis of the breast.We selected 12 patients with fibromatosis of the breast who underwent surgical resection and were confirmed by histological examination. Ultrasonography findings for 10 patients were reviewed and only two cases were suspicious for fibromatosis on imaging. On core needle biopsy for pre-operative diagnoses, only three cases were histologically suspicious for fibromatosis. Mutations in exon 3 of CTNNB1 were detected by direct DNA sequencing in nine (75.0%) cases: all were c.121GA (p.T41A), which was much more frequent in breast fibromatoses than in other soft tissue lesions. Nuclear β-catenin expression was observed in all cases and the level of expression was higher in cases with mutation. In eight of nine cases, the matched biopsy specimen showed the same CTNNB1 mutation status as the pre-operative specimen.In the majority of cases, clinical presentation and breast imaging are highly suspicious for carcinoma. Definitive pre-operative pathological diagnosis by core needle biopsy is difficult. CTNNB1 mutation and nuclear β-catenin expression are frequently detected in sporadic breast fibromatoses, suggesting their potential as a useful tool to distinguish breast fibromatoses from other neoplasms.
- Published
- 2012
14. Hepatic Lymphoma Representing Iso-Signal Intensity on Hepatobiliary Phase, in Gd-EOB-DTPA-Enhanced MRI: Case Report
- Author
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Chul Hi Park, Eun Ah Jung, Tae Ran Ahn, and Yeo Eun Kim
- Subjects
Gadoxetic acid ,medicine.diagnostic_test ,business.industry ,Hepatic lymphoma ,Gd-EOB-DTPA ,Magnetic resonance imaging ,Intensity (physics) ,hemic and lymphatic diseases ,medicine ,Hepatobiliary phase ,Signal intensity ,T2 weighted ,Nuclear medicine ,business ,medicine.drug - Abstract
The liver is an extranodal organ that is frequently invaded by lymphomas, with most cases involving secondary lymphomas (1). The image findings of hepatic lymphoma have been reported as variable, ranging from single or multiple nodules to diffuse infiltrates (1, 2). It is known that hepatic lymphomas show low signal intensity on T1 weighted images, high signal intensity on T2 weighted images, and low signal intensity on hepatobiliary phase image, using hepatocyte-specific contrast agents (3, 4). Here, we report an atypical case of hepatic lymphoma showing iso-signal intensity on hepatobiliary phase of contrast-enhanced MRI using gadoxetic acid (Gd-EOB-DTPA, Primovist; Bayer Schering Pharma, Berlin, Germany).
- Published
- 2015
15. Prevalence of Mutations in Discoidin Domain-Containing Receptor Tyrosine Kinase 2 (DDR2) in Squamous Cell Lung Cancers in Korean Patients.
- Author
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Mi-Sook Lee, Eun Ah Jung, Sung Bin An, Yu Jin Kim, Doo-Yi Oh, Ji-Young Song, Sang-Won Um, Joungho Han, and Yoon-La Choi
- Subjects
- *
PROTEIN-tyrosine kinases , *PROTEIN kinases , *LUNG cancer , *CANCER patients , *SQUAMOUS cell carcinoma , *DISCOIDIN domain receptor 2 - Abstract
Purpose The discoidin domain-containing receptor tyrosine kinase 2 (DDR2) is known to contain mutations in a small subset of patients with squamous cell carcinomas (SCC) of the lung. Studying the DDR2 mutations in patients with SCC of the lung would advance our understanding and guide the development of therapeutic strategies against lung cancer. Materials and Methods We selected 100 samples through a preliminary genetic screen, including specimens from biopsies and surgical resection, and confirmed SCC by histologic examination. DDR2mutations on exons 6, 15, 16, and 18 were analyzed by Sanger sequencing of formalin-fixed, paraffin-embedded tissue samples. The functional effects of novel DDR2mutants were confirmed by in vitro assays. Results We identified novel somatic mutations of DDR2 in two of the 100 SCC samples studied. One mutation was c.1745T>A (p.V582E) and the other was c.1784T>C (p.L595P), and both were on exon 15. Both patients were smokers and EGFR/KRAS/ALK-triple negative. The expression of the mutant DDR2 induced activation of DDR2 by the collagen ligand and caused enhanced cell growth and tumor progression. Moreover, dasatinib, a DDR2 inhibitor, showed potential efficacy against DDR2 L595P mutant-bearing cells. Conclusion Our results suggest that a mutation in DDR2 occurs naturally with a frequency of about 2% in Korean lung SCC patients. In addition, we showed that each of the novel DDR2mutations were located in a kinase domain and induced an increase in cell proliferation rate. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
16. Efficient expression of the vascular endothelial growth factor gene in vitro and in vivo, using an adeno-associated virus vector
- Author
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Sun-Jin Park, Eun-Ah Jung, Jean-Michel Heard, Duk-Kyung Kim, Jonghoe Byun, Jeong-Eun Huh, Hyeon-Cheol Gwon, and Jin Ok Jeong
- Subjects
Male ,Vascular Endothelial Growth Factor A ,Umbilical Veins ,DNA, Complementary ,Time Factors ,Angiogenesis ,viruses ,Genetic enhancement ,Genetic Vectors ,Guinea Pigs ,Neovascularization, Physiologic ,Vascular permeability ,Endothelial Growth Factors ,Biology ,medicine.disease_cause ,Transfection ,Cell Line ,Capillary Permeability ,Rats, Sprague-Dawley ,chemistry.chemical_compound ,Mice ,Ischemia ,Transduction, Genetic ,medicine ,Animals ,Humans ,Molecular Biology ,Adeno-associated virus ,Cells, Cultured ,Lymphokines ,Mice, Inbred BALB C ,Models, Genetic ,Vascular Endothelial Growth Factors ,Gene Transfer Techniques ,Extremities ,Dependovirus ,Molecular biology ,Rats ,Vascular endothelial growth factor ,Vascular endothelial growth factor A ,chemistry ,Regional Blood Flow ,Culture Media, Conditioned ,Female ,Expression cassette ,Endothelium, Vascular ,Cardiology and Cardiovascular Medicine ,HeLa Cells - Abstract
Vascular endothelial growth factor (VEGF) has proven to be one of the most effective growth factors for therapeutic angiogenesis. The biological efficacy of the adeno-associated virus (AAV) vector has recently been demonstrated in muscle tissues, including the heart. Apart from these promising insights into VEGF and the AAV vector, studies on VEGF gene transfer using the AAV vector have been limited. Here, we evaluate AAV-mediated VEGF gene transfer, both in vitro and in vivo, using the AAV-mVEGF vector that contains cDNA for murine VEGF(120) within an HCMV-driven expression cassette. Transient transfection of AAV-mVEGF plasmid significantly increased mVEGF expression in 293T cells. The secreted VEGF in the conditioned medium had strong biological activity, as confirmed by the Miles' vascular permeability assay. Transduction of 293T and HeLa cells with AAV-mVEGF stock of high titer, that is essentially adenovirus-free, showed significantly increased mVEGF expression above that of AAV-eGFP-transduced cells. When human umbilical vein endothelial cells were transduced, a higher level of mVEGF expression, together with higher cell counts, was observed compared to AAV-eGFP-transduced cells. In vivo transduction of mouse tibialis anterior muscle resulted in an increased level of mVEGF expression, and higher capillary-to-myofibre ratio, 8 weeks post-transduction. In a rat hindlimb ischemia model, regional blood flow, as well as the capillary-to-myofibre ratio, was significantly increased at 4 weeks post-transduction. These findings demonstrate the efficient delivery of the VEGF gene using an AAV vector, which has implications for angiogenic gene therapy in ischemic diseases.
- Published
- 2001
17. Intestinal bacterial metabolism of flavonoids and its relation to some biological activities
- Author
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In-Suk Sohng, Jung-Ah Han, Eun-Ah Jung, Dong-Hyun Kim, Myung Joo Han, and Tae-Hyung Kim
- Subjects
Cell Survival ,chemistry.chemical_compound ,Hesperidin ,Rutin ,Phenols ,Drug Discovery ,Tumor Cells, Cultured ,Humans ,heterocyclic compounds ,Glycosides ,Daidzin ,Naringin ,Biotransformation ,Poncirin ,Flavonoids ,biology ,Bacteria ,Organic Chemistry ,biology.organism_classification ,Intestines ,chemistry ,Biochemistry ,Molecular Medicine ,Drug Screening Assays, Antitumor ,Quercetin ,Trypsin Inhibitors ,Baicalin ,Platelet Aggregation Inhibitors - Abstract
Flavonoid glycosides were metabolized to phenolic acids via aglycones by human intestinal microflora producing alpha-rhamnosidase, exo-beta-glucosidase, endo-beta-glucosidase and/or beta-glucuronidase. Rutin, hesperidin, naringin and poncirin were transformed to their aglycones by the bacteria producing alpha-rhamnosidase and beta-glucosidase or endo-beta-glucosidase, and baicalin, puerarin and daidzin were transformed to their aglycones by the bacteria producing beta-glucuronidase, C-glycosidase and beta-glycosidase, respectively. Anti-platelet activity and cytotoxicity of the metabolites of flavonoid glycosides by human intestinal bacteria were more effective than those of the parental compounds. 3,4-Dihydroxyphenylacetic acid and 4-hydroxyl-phenylacetic acid were more effective than rutin and quercetin on anti-platelet aggregation activity. 2,4,6-Trihydroxybenzaldehyde, quercetin and ponciretin were more effective than rutin and ponciretin on the cytotoxicity for tumor cell lines. We insist that these flavonoid glycosides should be natural prodrugs.
- Published
- 1999
18. Coexistence of Intrapulmonary Bronchogenic Cyst and Congenital Cystic Adenomatoid Malformation - A Case Report
- Author
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Ji-Hye Lee, Hyun Deuk Cho, Eun Ah Jung, Seock Yeol Lee, Ki Hyun Seo, Mee-Hye Oh, and Young Tong Kim
- Subjects
Pathology ,medicine.medical_specialty ,Lung ,Pseudostratified ciliated columnar epithelium ,business.industry ,Cartilage ,Bronchogenic cyst ,Anatomy ,medicine.disease ,Pathology and Forensic Medicine ,Gross examination ,Cystic lesion ,medicine.anatomical_structure ,medicine ,Congenital Cystic Adenomatoid Malformation ,Respiratory epithelium ,business - Abstract
Congenital cystic lesions of the lung are uncommon and a conjunction of two or more lesions is very rare. We report here on a case of coexisting intrapulmonary bronchogenic cyst and congenital cystic adenomatoid malformation in a 13-year-old female with a cystic mass in the right upper lobe of the lung. Computed tomography showed a cystic lesion measuring 2.5 cm with an air flu id level and surrounding multicystic lesions in the right upper lobe. On gross examination, the cut surface showed a cystic mass containing inspissated mucinous material, and the cystic mass was surrounded by multiple small cysts. Microscopically, the larger cystic cavity was lined with pseudostratified ciliated columnar epithelium. The submucosal tissue contained mucinous glands and plates of cartilage. The surrounding smaller cysts or irregular spaces were lined with bronchiolartype respiratory epithelium. We propose that this hybrid lung lesion may represent the missing link in a common embryologic pathway determined by the timing of mesenchymal and epithelial interactions.
- Published
- 2011
19. Spindle Cell Epithelioma, a So-called Benign Mixed Tumor of the Vagina - A Case Report
- Author
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Eun Ah Jung, Seung-Ha Yang, Mee-Hye Oh, Ji-Hye Lee, Hyun Deuk Cho, and Jeong Ja Kwak
- Subjects
Cuboidal Cell ,Pathology ,medicine.medical_specialty ,Mixed tumor ,Epithelioma ,Vaginal neoplasm ,Anatomy ,Histogenesis ,Biology ,medicine.disease ,Squamous metaplasia ,Pathology and Forensic Medicine ,medicine.anatomical_structure ,medicine ,Vagina ,Mitosis - Abstract
We report a case of spindle cell epithelioma, a so-called benign mixed tumor of the vagina. The patient was a 35-year-old iparous woman who presented with a polypoid mass in the posterior wall of the lower vagina just above the hymenal ring. The tumor was relatively welldefined with an expansile margin and composed of stromal-type spindle cells with a myxoid stroma arranged in short fascicles and an irregular cord-like or reticular pattern. A few glandular structures lined by cuboidal cells with squamous metaplasia were also noted. Cellular atypia was not conspicuous and mitosis was not found. We examined this tumor immunohistochemically to identify the histogenesis. The coexpression of epithelial and mesenchymal markers in stromal-type spindle cells suggested a multipotential cell origin for this tumor. The patient has been well with no signs of recurrence during the 12 months after surgery.
- Published
- 2010
20. Clinicopathological Significance of S100A4 Expression in Non-small Cell Lung Carcinomas
- Author
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Mee-Hye Oh, Hyun Deuk Cho, Ji-Hye Lee, and Eun Ah Jung
- Subjects
Oncology ,Pathology ,medicine.medical_specialty ,Tissue microarray ,Lung ,business.industry ,Cell ,medicine.disease ,Pathology and Forensic Medicine ,Metastasis ,Log-rank test ,medicine.anatomical_structure ,Internal medicine ,medicine ,Immunohistochemistry ,Stage (cooking) ,business ,Survival analysis - Abstract
Background : S100A4 has been implicated in invasion and metastasis of various malignant tumors. The aim of this study was to investigate whether or not S100A4 plays an important role in non-small cell lung carcinomas (NSCLCs). Methods : Sixty-seven patients with NSCLC including 37 with squamous cell carcinomas (SCCs) and 30 with adenocarcinomas (ADs) who had undergone surgical resection were analyzed. S100A4 expression was analyzed by immunohistochemistry using tissue microarray blocks. Results : S100A4 expression was positive in 56 (83.6%) of 67 NSCLC cases. ADs were more frequently S100A4 positive than SCCs (p = 0.017). However, no significant correlation was observed between S100A4 expression and age, gender, pT, pN or tumor, node and metastasis (TNM) stage. Two distant metastatic cases revealed an S100A4 positive reaction. Kaplan-Meier survival curves with the logrank test showed no correlation with 3-year survival (p = 0.782) or 5-year survival (p = 0.227) in either group of patients according to S100A4 expression. Conclusions : S100A4 expression was not correlated with age, gender, pT, pN or TNM stage or survival in patients with NSCLCs. Therefore, S100A4 expression may not be useful as a prognostic marker for NSCLCs. However, S100A4 expression showed a higher positivity in ADs than in SCCs.
- Published
- 2010
21. Acute Respiratory Failure Caused by Aspiration of High Density Barium: A Case Report
- Author
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Yul Lee, Ki Soon Park, Soo Young Chung, Ik Yang, and Eun Ah Jung
- Subjects
medicine.medical_specialty ,chemistry ,business.industry ,Medicine ,chemistry.chemical_element ,High density ,Acute respiratory failure ,Barium ,business ,Intensive care medicine - Published
- 1994
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