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The Little Ice Age (LIA), AD 1350–1850, represents one of the most recent, persistent global climate oscillations. In Mexico, it has been associated with temperature decreases of 1.5–2 °C and mountain glacier advances, which are not accurately dated. We present new information about the nature of the LIA in central Mexico based on a decadal-resolution sediment sequence from high-altitude, tropical Lake La Luna, in the Nevado de Toluca volcano. We inferred past climatic and environmental changes using magnetic susceptibility, charcoal particles, palynomorphs, diatoms, cladoceran remains and multivariate statistics. The onset of the LIA corresponds with the beginning of a long-term trend to colder and drier climate ca. AD 1360–1910. The coolest and driest episode, ~AD 1660–1760, which corresponds with the Maunder Minimum in solar activity, was characterized by a cladoceran assemblage that showed the greatest dissimilarity to the modern one (no modern analogue), with the presence of cold-water species and Daphnia ephippia. The beginning of a warming trend ca. AD 1760, was identified by a diatom assemblage dominated by species with affinities for higher pH values (>6) and the greatest dissimilarity to the modern assemblage. This less cold, but still dry period, corresponds with historical reports of cattle and crop losses that predated the Mexican wars of Independence (AD 1810–1821) and Revolution (1910–1924). Modern conditions, established around AD 1910, resemble those during the Medieval Climate Anomaly (ca. AD 1200). No clear evidence of modern, human-induced environmental change was recorded, indicating that Lake La Luna is an ideal site in Mexico to monitor future impacts of global change.

High-altitude lakes, which are very sensitive ecosystems and respond rapidly to climatic changes, are one of the best targets for palaeolimnological studies. Here, we present the record of environmental changes over the last millennium that are recorded in the sediments of El Sol, a tropical, high-altitude, volcanic crater lake on the Nevado de Toluca, Central Mexico. Palaeolimnological reconstructions are based on subfossil Cladocera, diatoms, magnetic susceptibility and chemical analysis of the sediments. In general, Cladocera occurring in tropical regions, and especially at high altitude, have been studied very little. Our data indicate that in the sequence studied, the species diversity of subfossil Cladocera is very low. Only four species were recorded, and the assemblage is dominated by littoral species. Two Cladocera taxa, Alona manueli and Ilyocryptus , found at Lake El Sol are endemic. Cladocera, diatoms and the sediment chemistry show changes in Lake El Sol which are mostly related to climate. The most pronounced climatic signal was obtained for the early Little Ice Age (1350–1625 AD). This cold episode was expressed by a reduction in the frequency of zooplankton (individuals/cm 2 /yr) and diatoms (valves/g of dry sediment) and by changes in the organic carbon content in the sediment. Our results show that human activity was very limited throughout the study period. According to historical data the presence of humans at the lake shore was mostly occasional, usually for ceremonial and ritual purposes, and humans did not have an important influence on the lake ecosystem. Only one period was identified when human activity was important. This period corresponds to the introduction of fish into the lake at the beginning of the 20th century.

Lagunillo del Tejo is a small groundwater-fed sinkhole lake in the karst region of the Iberian Range (central-eastern Spain), which undergoes significant lake level fluctuation in response to rainfall variability. The aim of this study is to understand the record of water level fluctuations in Lagunillo del Tejo over the last two-and-a-half centuries. This information could be used in future studies to interpret longer sedimentary sequences. We analysed photosynthetic pigments, diatoms and cladoceran remains in sediment sequences recovered from the deepest part of the lake. The paleoecological proxies traced two different communities which have switched their prevalence during the past: (1) a planktonic community of algae, including diatoms, chlorophytes, cryptophytes and cyanobacteria, and phototrophic bacteria associated with higher lake level and water column seasonal stratification; (2) a littoral community with the higher levels of macrophyte pigments and associated epiphytic diatoms and chydorids, all of which indicate lower lake level. The levels of coherence between different proxies, each having an independent mechanistic link to lake-level variability, enhance the reliability of palaeolimnological inferences. The high-resolution stratigraphical data from the upper part of the core was compared with lake-level inferences from instrumental rainfall series (1859–2005) to establish the correspondence between Lagunillo del Tejo sediment sequences and climate record.