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BACKGROUND: The primary objective of this trial is to examine the mechanisms of time-restricted eating (TRE) as an adjunct to psychiatric care for people with bipolar disorder (BD) with sleep or circadian disruptions. This study builds on prior studies of circadian disruption in BD as well as growing evidence that TRE improves circadian functioning. METHODS: One-hundred fifty participants diagnosed with BD 1 or II will be recruited via advertising in the local community. Main inclusion criteria include: obtaining medical treatment for BD; current sleep or circadian problems; self-reported eating period of ≥ 12 h; no eating disorder or other health conditions that would hinder or limit the safety of following TRE; and not currently experiencing a mood episode, acute suicidality, psychosis, alcohol or substance use disorder. Participants will be asked to complete a baseline period in which daily food intake is logged online for two weeks. After baseline, participants will be asked to follow TRE for 8 weeks and to continue to complete daily food logging during this time. Symptom severity interviews will be conducted by phone or videoconference at baseline, mid-intervention (6 weeks post-baseline), end of intervention (10 weeks post-baseline), and 6 months post-baseline. Self-rated symptom severity and quality of life data will be gathered online at the same time points as symptom severity interviews, and at 16 weeks post-baseline (6 weeks after the TRE period ends). To assess potential mechanisms of change, we will examine the change in diurnal amplitude of clock gene expression as a primary mediator at 8 weeks compared to baseline. We will further test whether diurnal amplitude of clock gene expression is predictive above and beyond the role of two covariate potential mediators, glucose tolerance and inflammation at 8 weeks relative to baseline. To provide an index of whether TRE successfully decreases emotional lability, participants will be asked to complete 5 mood assessments per day for 7 days at baseline and at 10 weeks. These mood assessments will be optional. DISCUSSION: The planned research will provide novel and important information on whether TRE improves sleep/circadian rhythm problems, along with reductions in mood symptoms and improvements in quality of life, for individuals with BD. TRIAL REGISTRATION: ClinicalTrials.gov ID: NCT06555406.

Circadian regulation of autonomic tone and reflex pathways pairs physiological processes with the daily light cycle. However, the underlying mechanisms mediating these changes on autonomic neurocircuitry are only beginning to be understood. The brainstem nucleus of the solitary tract (NTS) and adjacent nuclei, including the area postrema and dorsal motor nucleus of the vagus, are key candidates for rhythmic control of some aspects of the autonomic nervous system. Recent findings have contributed to a working model of circadian regulation in the brainstem which manifests from the transcriptional, to synaptic, to circuit levels of organization. Vagal afferent neurons and the NTS possess rhythmic clock gene expression, rhythmic action potential firing, and our recent findings demonstrate rhythmic spontaneous glutamate release. In addition, postsynaptic conductances also vary across the day producing subtle changes in membrane depolarization which govern synaptic efficacy. Together these coordinated pre‐ and postsynaptic changes provide nuanced control of synaptic transmission across the day to tune the sensitivity of primary afferent input and likely govern reflex output. Further, given the important role for the brainstem in integrating cues such as feeding, cardiovascular function and temperature, it may also be an underappreciated locus in mediating the effects of such non‐photic entraining cues. This short review focuses on the neurophysiological principles that govern NTS synaptic transmission and how circadian rhythms impacted them across the day. What is the topic of this review?Synaptic encoding of brainstem circadian rhythms.What advances does it highlight?Rhythmic changes in fast neurotransmission span the time frames between transcription/translation and physiological/behavioural responses. Coordinated pre‐ and postsynaptic neurophysiological changes provide nuanced circadian control to synaptic throughput. Spontaneous vesicle release is a unique cellular mechanism for encoding circadian changes to synaptic and circuit function. [ABSTRACT FROM AUTHOR]

Diurnal gene expression is a pervasive phenomenon occurring across all kingdoms of life, orchestrating adaptive responses to daily environmental fluctuations and thus enhancing organismal fitness. Our understanding of the plant circadian clock is primarily derived from studies in Arabidopsis , and direct comparisons are difficult due to differences in gene family sizes. To this end, the identification of functional orthologs based on diurnal and tissue expression is necessary. The diurnal.plant.tools database constitutes a repository of gene expression profiles from 17 members of the Archaeplastida lineage, with built-in tools facilitating cross-species comparisons. In this database update, we expand the dataset with diurnal gene expression from four agriculturally significant crop species and Marchantia , a plant of evolutionary significance. Notably, the inclusion of diurnal gene expression data for Marchantia enables researchers to glean insights into the evolutionary trajectories of the circadian clock and other biological processes spanning from algae to angiosperms. Moreover, integrating diurnal gene expression data with datasets from related gene co-expression databases, such as CoNekt-Plants and CoNekt-Stress, which contain gene expression data for tissue and perturbation experiments, provides a comprehensive overview of gene functions across diverse biological contexts. This expanded database serves as a valuable resource for elucidating the intricacies of diurnal gene regulation and its evolutionary underpinnings in plant biology. [ABSTRACT FROM AUTHOR]

The near-surface air temperature (${{T}_{air}}$) is a principal variable describing energy exchange and water circulation between the land surface and the atmospheric environment. The estimation of ${{T}_{air}}$ by satellite land surface temperature (LST) is challenging due to the variable magnitude of the difference between ${{T}_{air}}$ and LST in both space and time, as well as the restriction of estimated ${{T}_{air}}$ to clear-sky conditions because of the penetration of infrared wavelengths. Moreover, the estimation suffers from low temporal resolution and primarily focuses on daily minimum, maximum, and two instantaneous ${{T}_{air}}$ per day. This study proposes a method for estimating all-sky gridded diurnal ${{T}_{air}}$ at regional scale from FY-4B/AGRI measurements. The multiscale geographically weighted regression model was investigated to establish the dynamic relationships between ground station observed ${{T}_{air}}$ and satellite LST under clear-sky conditions by employing different spatial values for each explanatory variable in localized regressions. A moving window loop based multiple linear regression was employed to establish the relationship between satellite-derived clear-sky ${{T}_{air}}$ and other variables to extrapolate ${{T}_{air}}$ in cloudy-sky pixels. The results showed that the proposed method captures the trend of ${{T}_{air}}$ variations well in hourly profiles with R values greater than 0.95. RMSE was 1.75 °C, 1.38 °C, 1.95 °C, and 2.19 °C in April, July, October, and January, respectively. The demonstration of heatwave monitoring showed that satellite-estimated ${{T}_{air}}$ provide an excellent representation of the spatial and temporal evolution of the heatwave.

Abstract Circadian regulation of autonomic tone and reflex pathways pairs physiological processes with the daily light cycle. However, the underlying mechanisms mediating these changes on autonomic neurocircuitry are only beginning to be understood. The brainstem nucleus of the solitary tract (NTS) and adjacent nuclei, including the area postrema and dorsal motor nucleus of the vagus, are key candidates for rhythmic control of some aspects of the autonomic nervous system. Recent findings have contributed to a working model of circadian regulation in the brainstem which manifests from the transcriptional, to synaptic, to circuit levels of organization. Vagal afferent neurons and the NTS possess rhythmic clock gene expression, rhythmic action potential firing, and our recent findings demonstrate rhythmic spontaneous glutamate release. In addition, postsynaptic conductances also vary across the day producing subtle changes in membrane depolarization which govern synaptic efficacy. Together these coordinated pre‐ and postsynaptic changes provide nuanced control of synaptic transmission across the day to tune the sensitivity of primary afferent input and likely govern reflex output. Further, given the important role for the brainstem in integrating cues such as feeding, cardiovascular function and temperature, it may also be an underappreciated locus in mediating the effects of such non‐photic entraining cues. This short review focuses on the neurophysiological principles that govern NTS synaptic transmission and how circadian rhythms impacted them across the day.

Abstract Background The primary objective of this trial is to examine the mechanisms of time-restricted eating (TRE) as an adjunct to psychiatric care for people with bipolar disorder (BD) with sleep or circadian disruptions. This study builds on prior studies of circadian disruption in BD as well as growing evidence that TRE improves circadian functioning. Methods One-hundred fifty participants diagnosed with BD 1 or II will be recruited via advertising in the local community. Main inclusion criteria include: obtaining medical treatment for BD; current sleep or circadian problems; self-reported eating period of ≥ 12 h; no eating disorder or other health conditions that would hinder or limit the safety of following TRE; and not currently experiencing a mood episode, acute suicidality, psychosis, alcohol or substance use disorder. Participants will be asked to complete a baseline period in which daily food intake is logged online for two weeks. After baseline, participants will be asked to follow TRE for 8 weeks and to continue to complete daily food logging during this time. Symptom severity interviews will be conducted by phone or videoconference at baseline, mid-intervention (6 weeks post-baseline), end of intervention (10 weeks post-baseline), and 6 months post-baseline. Self-rated symptom severity and quality of life data will be gathered online at the same time points as symptom severity interviews, and at 16 weeks post-baseline (6 weeks after the TRE period ends). To assess potential mechanisms of change, we will examine the change in diurnal amplitude of ‘clock’ gene expression as a primary mediator at 8 weeks compared to baseline. We will further test whether diurnal amplitude of clock gene expression is predictive above and beyond the role of two covariate potential mediators, glucose tolerance and inflammation at 8 weeks relative to baseline. To provide an index of whether TRE successfully decreases emotional lability, participants will be asked to complete 5 mood assessments per day for 7 days at baseline and at 10 weeks. These mood assessments will be optional. Discussion The planned research will provide novel and important information on whether TRE improves sleep/circadian rhythm problems, along with reductions in mood symptoms and improvements in quality of life, for individuals with BD. Trial registration ClinicalTrials.gov ID: NCT06555406.

Accumulation of edible biomass by crop plants relies on maintenance of a high photosynthetic rates across the photoperiod, with assimilation rate (A) generally responding to increasing light intensity in a hyperbolic fashion. In natural environments light fluctuates greatly over the course of the day, however in Controlled Environmental Agricultural (CEA) systems, light intensity can be supplemented or precisely controlled using LEDs to create near optimum conditions. In such indoor growth environments light is often delivered as a square wave and recommendations to horticulturalists are given in the form of Daily Light Integrals (DLI). However, this does not take into account the slow photosynthetic induction at the start of the photoperiod and the decline of A towards the end of the photoperiod, which has been demonstrated by several previous studies. Square wave light regimes therefore potentially cause suboptimal photosynthetic utilization of the applied lighting and waste electricity. Here we have adapted light recipes to gradually increase and decrease in intensity to take account of these findings. We demonstrate that, utilising a sinusoidal light regime capped at 250 mm m-² s-1, it is possible to increase edible biomass of rocket (by ca. 20%) compared to square wave delivered at 250 at the same DLI. Additionally, this can be achieved using less electricity (0.6%), therefore reducing energy costs and improving profitability. We suggest that capping maximum light intensity at 250 µmol m-² s-¹ improves the operating efficiency of PSII photochemistry (Fq'/Fm') also known as the photosynthetic efficiency by maintaining A later in the photoperiod. We show that a higher electron transfer rate (ETR) is maintained in these treatments over the photoperiod compared to higher light intensity caps, resulting in a greater Daily Photochemical Integral (DPI). We attribute this to less NPQ due to a greater sink capacity for the end products of electron transport, ATP and NADPH, as A is kept high for longer. [ABSTRACT FROM AUTHOR]

Analyses of global climate model results for urban impacts on temperature and precipitation are rare. Previous analyses of the global Conformal Cubic Atmospheric Model simulation results for 1985–2010 have revealed urban effects on minimum and maximum temperatures. Using the same dataset to derive time‐zone‐corrected three‐hourly local time period (LTP) data, averaged diurnal cycles of temperature and precipitation were calculated for grid cells with greater than 10% urban fraction (urban grid cells) globally. Three latitudinal bands were assessed: northern extratropics (NET, 274 urban grid cells), southern extratropics (SET, 39 urban grid cells), and the Tropics (26 urban grid cells). The largest statistically significant urban influences on temperature are consistently found at night, in agreement with many previous studies on urban heat islands. Signs of urban cooling were found for a few hours, from 09:00 to 15:00 LTP, most often in summer. Influences of urban areas on precipitation varied, with small increases and decreases in all latitudinal bands and seasons. For NET, increases were generally found. In the Tropics, increases were found from 21:00 to 09:00 LTP for all seasons except DJF, with decreases for all seasons from 15:00 to 18:00 LTP. In SET, all seasons had increases for 21:00 to 00:00 LTP and decreases for 15:00 to 18:00 LTP. DJF had decreases for all LTPs except 21:00 to 00:00 LTP and SON had increases for all times except 15:00 to 18:00 LTP. Differences in rainfall in the region surrounding the urban areas were broadly similar to local changes in NET. For the Tropics and SET, regional decreases were found for DJF and JJA, with a more varied pattern for other months. Regional effects appeared to be more restricted to near‐urban areas in the Tropics than in NET. The results indicate some influence of nearby urban areas on regional temperature and precipitation. [ABSTRACT FROM AUTHOR]

Increasing age and puberty affect the hypothalamic pituitary adrenal (HPA) axis maturation, which is likely associated with an increase in environmental demands (e.g. social) and vulnerability for the onset of psychiatric conditions (e.g. depression). There is limited research as to whether such patterns are consonant in youth with autism spectrum disorder (ASD), a condition marked by social challenges, dysregulation of the HPA axis, and higher rates of depression setting the stage for enhanced vulnerability during this developmental period. The current study interrogated diurnal cortisol by examining (1) cortisol expression longitudinally over the pubertal transition between autistic and neurotypical youth, (2) the trajectory of diurnal cortisol and the unique contributions of age vs. puberty, and (3) potential sex differences. As hypothesized, results indicate autistic compared to typically developing youth demonstrate a shallower diurnal slope and elevated evening cortisol. These differences were in the context of higher cortisol and flatter rhythms based on age and pubertal development. Also, sex-based differences emerged such that females in both groups had higher cortisol, flatter slopes, and higher evening cortisol than males. The results show that despite the trait-like stability of diurnal cortisol, HPA maturation is impacted by age, puberty, sex, as well as an ASD diagnosis. [ABSTRACT FROM AUTHOR]

Circadian clocks are inherent to most organisms, including cryptozoic animals that seldom encounter direct light, and regulate their daily activity cycles. A conserved suite of clock genes underpins these rhythms. In this study, we explore the circadian behaviors of the red flour beetle Tribolium castaneum, a significant pest impacting stored grain globally. We report on how daily light and temperature cues synchronize distinct activity patterns in these beetles, characterized by reduced morning activity and increased evening activity, anticipating the respective environmental transitions. Although less robust, rhythmicity in locomotor activity is maintained in constant dark and constant light conditions. Notably, we observed more robust rhythmic behaviors in males than females with individual variation exceeding those previously reported for other insect species. RNA interference targeting the Clock gene weakened locomotor activity rhythms. Our findings demonstrate the existence of a circadian clock and of clock-controlled behaviors in T. castaneum. Furthermore, they highlight substantial individual differences in circadian activity, laying the groundwork for future research on the relevance of individual variation in circadian rhythms in an ecological and evolutionary context. [ABSTRACT FROM AUTHOR]

Many unique high‐altitude mountain ecosystems have been declining due to climate change, posing a threat to flora and fauna that have adapted to these ecosystems. This study explores the evolution of high‐altitude adaptations, focusing on female flightlessness and diel activity, in geometrid moths (Lepidoptera: Ennominae, Gnophini) within the European Alps. We constructed a phylogeny of Gnophini moths using a dataset of 157 taxa, with up to seven genetic markers and traced the evolutionary history of diel activity and wing length reduction in females. Analysis of divergence times suggested that female flightlessness has evolved at least three times independently between the early and late Miocene. The evolution of wing length reduction is likely correlated with elevation, indicating adaptations to cold and windy conditions in high altitude. The evolutionary events leading to shifts in adult diel activity, from ancestral nocturnality to diurnality, have occurred independently at least three times and may also be a consequence of adaptations at high elevations. Strikingly, among diurnal Sciadia, two species have evolved further to become nocturnal like their ancestors. Our findings highlight how phylogenies can provide new insights into evolutionary patterns in moths. We provided a robust basis for resolving taxonomic ambiguities in Alpine Gnophini, leading us to propose 10 changes to the current classification: Scrupodes Lee & Sihvonen gen. n., Elophos Boisduval (type species Geometra operaria Hübner) is considered a junior synonym of Sciadia Hübner syn. n., Yezognophos dilucidaria (Denis & Schiffermüller) and Y. sproengertsi (Püngeler) are transferred to Parietaria Leraut comb. n., Yezognophos serotinaria (Denis & Schiffermüller) is transferred to Scrupeus Lee & Sihvonen comb. n., Elophos caelibaria (Heydenreich), E. zirbitzensis (Pieszcek), E. operaria (Hübner) and E. andereggaria (De La Harpe) are transferred to Sciadia Hübner comb. n. and Dichrognophos Wehrli (type species Gnophos orthogonia Wehrli) is transferred from Ennominae: Cassymini to Ennominae: Gnophini. [ABSTRACT FROM AUTHOR]

As many aspects of animal, human and plant behavior and survival are being perturbed by widespread exposure to artificial light at night (ALAN), lighting professionals are in the middle, attempting to make design decisions that directly affect these life forms. Research shows any light exposure higher than the nighttime natural environment will have a negative impact on various life forms during the nocturnal phase. This paper aims to provide a design process for "life-centric" lighting with good human and non-human outcomes for all life-sustaining behaviors on earth driven by phototropism, phototaxis, photoperiodism and circadian entrainment as the foundational consideration. Fundamental understandings of how light information is used by these life forms in a way crucial for development, growth and survival is provided in the paper. Key references are consolidated and synthesized using PRISMA 2020 with goals of supporting knowledge translation into pragmatic lighting strategies in a manner useful for lighting professionals. A process for life-centric lighting design using an existing process for human-centric lighting design involving lighting intensity and spectrum along with spatial and temporal patterns is provided, which can dovetail with decision-making processes that are within the workflow of lighting professionals. Well-designed studies with a wider range of light intensities and spectrum and well-defined lighting properties, along with better modes of communication between lighting professionals and ALAN researchers are recommended to facilitate the realization of biodynamic lighting solutions that will support good human and non-human outcomes. [ABSTRACT FROM AUTHOR]

Simple Summary: The way animals are housed and cared for in research is vital for both ethical reasons and the quality of research produced. One important aspect of mouse housing is air quality. In this study, we measured levels of two potentially harmful compounds, ammonia and carbon dioxide, as well as humidity and temperature, in mouse cages. This study followed the mice from adulthood (100 days old) to middle age (322 days old) as they gained 35–50% body weight. The mice were divided by sex, and their cages were cleaned either weekly (7 d) or bi-weekly (14 d). Our findings show that air quality remains within the safe levels for younger, lighter mice, but changes throughout the day, with higher levels of carbon dioxide, ammonia, and humidity when the mice are active at night. However, as the mice aged and gained weight, the ammonia and humidity levels increased, potentially reaching harmful levels. Carbon dioxide and temperature were less affected by these changes. Our study suggests that it is important to consider mice's activity patterns and body mass when determining the frequency of cage cleaning and housing density. Housing conditions are essential for ensuring animal welfare and high-quality research outcomes. In this study, we continuously monitored air quality—specifically ammonia, carbon dioxide, relative humidity, and temperature—in Individually Ventilated Cages (IVCs) housing five female or male C57BL/6N mice. The cages were cleaned either weekly or bi-weekly, and the data were collected as the mice aged from 100 to 348 days. The survival rate remained above 96%, with body weight increasing by 35–52% during the study period. The ammonia levels rose throughout the cleaning cycle, but averaged below 25 ppm. However, in the older, heavier mice with bi-weekly cage cleaning, the ammonia levels reached between 25 and 75 ppm, particularly in the males. While circadian rhythms influenced the ammonia concentration only to a small extent, the carbon dioxide levels varied between 800 and 3000 ppm, increasing by 30–50% at night and by 1000 ppm with body weight. Humidity also correlated primarily with the circadian rhythms (10% higher at night) and, to a lesser extent, with body weight, reaching ≥70% in the middle-aged mice. The temperature variations remained minimal, within a 1 °C range. We conclude that air quality assessments in IVCs should be conducted during animals' active periods, and both housing density and biomass must be considered to optimise welfare. [ABSTRACT FROM AUTHOR]

A pasture or concentrate-based dietary regime impacts a variety of factors including both ruminal health and function, and consequently milk production and quality. The objective of this study was to examine the effect of feeding differing pasture levels on the metabolite composition of bovine ruminal fluid. Ruminal fluid was obtained from rumen-cannulated spring-calving cows (N = 9, Holstein-Friesian breed, average lactation number = 5) fed one of three diets across a full lactation season. Group 1 (pasture) consumed perennial ryegrass supplemented with 5% concentrates; group 2 received a total mixed ration (TMR) diet; and group 3 received a partial mixed ration (PMR) diet which included pasture and a TMR. Samples were taken at two timepoints: morning and evening. Metabolomic analysis was performed using nuclear magnetic resonance (1H-NMR) spectroscopy. Statistical analysis revealed significant changes across the dietary regimes in both morning and evening samples, with distinct alterations in the metabolite composition of ruminal fluid from pasture-fed cows (FDR-adjusted p-value < 0.05). Acetate and butyrate were significantly higher in samples derived from a pasture-based diet whereas sugar-related metabolites were higher in concentrate-based samples. Furthermore, a distinct diurnal impact on the metabolite profile was evident. This work lays the foundation for understanding the complex interaction between dietary regime and ruminal health. [ABSTRACT FROM AUTHOR]

The diurnal cycles of near‐surface velocity and temperature, also known as diurnal jet and diurnal warm layer (DWL), are ubiquitous in the tropical oceans, affecting the heat and momentum budget of the ocean surface layer, air‐sea interactions, and vertical mixing. Here, we analyze the presence and descent of near‐surface diurnal shear and stratification in the upper 20 m of the equatorial Atlantic as a function of wind speed using ocean current velocity and hydrographic data taken during two trans‐Atlantic cruises along the equator in October 2019 and May 2022, data from three types of surface drifters, and data from Prediction and Research Moored Array in the Tropical Atlantic (PIRATA) moorings along the equator. The observations during two seasons with similar mean wind speeds but varying surface heat fluxes reveal similar diurnal jets with an amplitude of about 0.11 m s−1 and similar DWLs when averaging along the equator. We find that higher wind speeds lead to earlier diurnal peaks, deeper penetration depths, and faster descent rates of DWL and diurnal jet. While the diurnal amplitude of stratification is maximum for minimal wind speeds, the diurnal amplitude of shear is maximum at 6 m depth for moderate wind speeds of about 5 m s−1. The inferred wind dependence of the descent rates of DWL and diurnal jet is consistent with the earlier onset of deep‐cycle turbulence for higher wind speeds. The DWL and the diurnal jet not only trigger deep‐cycle turbulence but are also observed to modify the wind power input and thus the amount of energy available for mixing. Plain Language Summary: During daytime, solar radiation leads to the formation of a thin warm layer at the ocean surface which can trap heat and wind‐forced momentum. Both heat and momentum are transported in the deeper ocean during the evening and night by turbulent mixing. The associated diurnal variation of temperature, current velocity, and their vertical gradients, stratification and velocity shear, are thus relevant for understanding ocean‐atmosphere interactions. This study investigates how the diurnal variation in stratification and velocity shear is influenced by the wind speed. For that, basin‐scale observations of velocity and temperature, which were collected in the equatorial Atlantic during two trans‐Atlantic equatorial cruises and by instruments installed at long‐term moorings along the equator, are analyzed. These observations reveal that the wind speed influences the amplitude, the timing, and the vertical structure of the diurnal variation in stratification and velocity shear. Wind speed also influences how deep and how fast this variation propagates from the surface downward. The study concludes that the diurnal variation of stratification and velocity shear impacts first the input of mechanical energy from the atmosphere into the ocean and second the process of turbulent mixing below the night‐time mixed layer. Key Points: Basin‐scale in‐situ data show the evolution of diurnal warm layer and diurnal jet in the upper 15 m of the equatorial Atlantic OceanHigher wind speeds lead to earlier diurnal peaks, deeper penetration depths, and faster descent rates of the diurnal jetWind speed dependence of descent rates of diurnal shear and stratification can explain the varying onset of deep‐cycle turbulence [ABSTRACT FROM AUTHOR]

Preliminary evidence suggests that evening chronotype is related to poorer efficacy of selective serotonin reuptake inhibitors. It is unknown whether this is specific to particular medications, self-rated chronotype, or efficacy. In the Australian Genetics of Depression Study (n = 15,108; 75% women; 18–90 years; 68% with ≥1 other lifetime diagnosis), a survey recorded experiences with 10 antidepressants, and the reduced Morningness-Eveningness Questionnaire was used to estimate chronotype. A chronotype polygenic score was calculated. Age- and sex-adjusted regression models (Bonferroni-corrected) estimated associations among antidepressant variables (how well the antidepressant worked [efficacy], duration of symptom improvement, side effects, discontinuation due to side effects) and self-rated and genetic chronotypes. The chronotype polygenic score explained 4% of the variance in self-rated chronotype (r = 0.21). Higher self-rated eveningness was associated with poorer efficacy of escitalopram (odds ratio [OR] = 1.04; 95% CI, 1.02 to 1.06; p =.000035), citalopram (OR = 1.03; 95% CI, 1.01 to 1.05; p =.004), fluoxetine (OR = 1.03; 95% CI, 1.01 to 1.05; p =.001), sertraline (OR = 1.02; 95% CI, 1.01 to 1.04; p =.0008), and desvenlafaxine (OR = 1.03; 95% CI, 1.01 to 1.05; p =.004), and a profile of increased side effects (80% of those recorded; ORs = 0.93–0.98), with difficulty getting to sleep the most common. Self-rated chronotype was unrelated to duration of improvement or discontinuation. The chronotype polygenic score was only associated with suicidal thoughts and attempted suicide (self-reported). While our measures are imperfect, and not of circadian phase under controlled conditions, the model coefficients suggest that dysregulation of the phenotypic chronotype relative to its genetic proxy drove relationships with antidepressant outcomes. The idea that variation in circadian factors influences response to antidepressants was supported and encourages exploration of circadian mechanisms of depressive disorders and antidepressant treatments. [ABSTRACT FROM AUTHOR]

Individuals vary in their ability to taste, and some individuals are more sensitive to certain tastes than others. Taste sensitivity is a predictor of various factors, such as diet, eating behavior, appetite regulation, and overall health. Furthermore, taste sensitivity can fluctuate within an individual over short to long periods of time: for example, in daily (diurnal) cycles, monthly (menstrual) cycles (in females), and yearly (seasonal) cycles. Understanding these temporal patterns is important for understanding individual eating habits and food preferences, particularly in the context of personalized and precision nutrition. This review provides a summary of the literature on taste sensitivity patterns across 3 temporal dimensions: daily, monthly, and yearly. Good evidence for diurnal patterns has been observed for sweet taste and fat taste, although the evidence is limited to rodent studies for the latter. Obese populations showed limited variation to sweet and fat taste sensitivities over a day, with limited variation in sweet taste sensitivity being linked to insulin resistance. There were mixed observations of temporal variation in sensitivity to sour and umami tastes, and there were no patterns in sensitivity to bitter taste. Menstrual patterns in sweet taste sensitivity were consistent with patterns in food intake. Other taste modality investigations had mixed findings that had little agreement across studies. Hormonal changes in females influence taste sensitivity to some degree, although the overall patterns are unclear. Seasonal patterns have been less well studied, but there is weak evidence that sweet, salty, and bitter taste sensitivities change across seasons. Differences in seasonal taste patterns have been observed in subgroups susceptible to mental health disorders, requiring further investigation. Patterns of taste sensitivity are evident across multiple temporal dimensions, and more research is needed to determine the influence of these patterns on food intake. Dysregulation of these patterns may also be a marker of certain diseases or health conditions, warranting further investigation. Notably, the alimentary tastes (umami, fat, and carbohydrate) are underrepresented in this research area and require additional investigation. [ABSTRACT FROM AUTHOR]

Purpose: The aim of this study was to determine whether variations in technical and neuromuscular performance occur across different times of the day in basketball players. Methods: Twenty semiprofessional, female basketball players (23 ± 4 years) competing in a second-division national basketball competition completed separate testing batteries in the morning (08:30) and in the afternoon (17:30) in a randomized counterbalanced order. Testing sessions consisted of a free-throw accuracy test to assess technical performance, as well as flexibility (ankle dorsiflexion range-of-motion test), dynamic balance (modified star excursion balance test), vertical jump height (squat jump, countermovement jump with and without arm swing), strength (isometric handgrip), change-of-direction speed (V-cut test), and linear speed (20-m sprint) tests to assess neuromuscular performance. Mechanism variables were also obtained including tympanic temperature, urinary specific gravity, and rating of perceived exertion at each session. Results: Squat jump height (6.7%; p =.001; effect size (ES) = 0.33), countermovement jump height with (4.1%; p =.018; ES = 0.27) and without arm swing (5.9%; p =.007; ES = 0.30), and 20-m sprint time (−1.4%; p =.015; ES = -0.32) were significantly superior in the afternoon compared to morning. Tympanic temperature was significantly higher in the afternoon than morning (1.4%; p <.001; ES = 1.31). In contrast, no significant differences between timepoints were evident for all remaining variables (p >.05; ES = -0.33 to 0.16). Conclusions: Some neuromuscular variables exhibited a time-of-day effect with better jump and sprint performance in the afternoon compared to morning in semiprofessional, female basketball players. [ABSTRACT FROM AUTHOR]

Introduction: Transitions in temporal niche have occurred many times over the course of mammalian evolution. These are associated with changes in sensory stimuli available to animals, particularly with visual cues, because levels of light are so much higher during the day than at night. This relationship between temporal niche and available sensory stimuli elicits the expectation that evolutionary transitions between diurnal and nocturnal lifestyles will be accompanied by modifications of sensory systems that optimize the ability of animals to receive, process, and react to important stimuli in the environment. Methods: This study examines the influence of temporal niche on investment in sensory brain tissue of 13 rodent species (five diurnal; eight nocturnal). Animals were euthanized and the brains immediately frozen on dry ice; olfactory bulbs were subsequently dissected and weighed, and the remaining brain was weighed, sectioned, and stained. Stereo Investigator was used to calculate volumes of four sensory regions that function in processing visual (lateral geniculate nucleus, superior colliculus) and auditory (medial geniculate nucleus, inferior colliculus) information. A phylogenetic framework was used to assess the influence of temporal niche on the relative sizes of these brain structures and of olfactory bulb weights. Results: Compared to nocturnal species, diurnal species had larger visual regions, whereas nocturnal species had larger olfactory bulbs than their diurnal counterparts. Of the two auditory structures examined, one (medial geniculate nucleus) was larger in diurnal species, while the other (inferior colliculus) did not differ significantly with temporal niche. Conclusion: Our results indicate a possible indirect association between temporal niche and auditory investment and suggest probable trade-offs of investment between olfactory and visual areas of the brain, with diurnal species investing more in processing visual information and nocturnal species investing more in processing olfactory information. [ABSTRACT FROM AUTHOR]

We use the extensive stellar occultation data set of the Imaging Ultraviolet Spectrograph aboard the Mars Atmosphere and Volatile EvolutioN spacecraft to determine the first quantification of vertical variation in O2 mole fraction separately for day and night in the ∼90–130 km altitude range. The upper atmospheric O2 variation is expected to be due to the interplay between diffusion and advection because of its long photochemical lifetime. It is therefore a useful tracer of the state of atmospheric mixing and circulation. The altitude‐averaged mixing ratio is measured to be 2.69(±0.03) × 10−3 for the nightside and 2.05(±0.03) × 10−3 for the dayside. The average O2 mole fraction for day and night are nearly identical below 105 km, consistent with the value of 1.61 × 10−3 derived from the Mars Curiosity Rover/Sample Analysis at Mars near‐surface measurements. At higher altitudes, dominated by molecular diffusive separation, the measured O2 mole fraction demonstrates a vertical gradient with a local time dependence. The nightside mole fraction is a factor of 1.37 ± 0.04 larger than the dayside value at ∼125 km. This nightside enhancement is explained in terms of the relative role of solar‐driven rapid horizontal winds at high altitudes and slower vertical diffusion, resulting in a nightside (dayside) downward (upward) diffusive flux. Using the 1‐D diffusion model, the measured profiles correspond to a vertical eddy diffusion coefficient K = 3.5(±1.5) × 106 cm2/s. The Mars Climate Database predicts comparable but lower day‐night differences in oxygen mole fraction due to an overestimated K = 7.0(±1.0) × 106 cm2/s, which affects atmospheric mixing as well as the rate of atmospheric escape to space. Plain Language Summary: Molecular oxygen in the martian atmosphere is produced photochemically and behaves like an inert trace gas due to its long photochemical lifetime (∼60 Earth years). Its variations in the upper atmosphere are therefore governed by diffusion and dynamics and are indicative of the changes in atmospheric mixing and circulation. Other data sets have only provided a glimpse of O2 abundance in the upper atmosphere due to limited spatial and temporal coverage. The extensive stellar occultation data set of the Imaging Ultraviolet Spectrograph aboard the Mars Atmosphere and Volatile EvolutioN spacecraft can help fill this knowledge gap. This study shows the first quantification of vertical variation in O2 mixing ratio separately for day and night in the ∼90–130 km altitude range. While the day and night O2 mixing ratio is measured to be identical below 105 km in the well‐mixed atmosphere, significant local time dependence is seen under molecular diffusive separation, with a nightside enhancement and a dayside depletion. This is explained in terms of diffusive flux from the dayside to the nightside. However, the Mars Climate Database output is inconsistent with these measurements, which therefore have important implications for how species diffuse through the martian atmosphere and escape to space. Key Points: Stellar occultations observed by Mars Atmosphere and Volatile EvolutioN/Imaging Ultraviolet Spectrograph allow the most complete study of molecular oxygen in the upper atmosphereSignificant day/night differences reveal the importance of diurnal circulation and molecular diffusionObserved day/night differences exceed those in models, suggesting mixing is significantly overestimated in those models [ABSTRACT FROM AUTHOR]

Joss-Waldvogel Disdrometer (JWD) measurements at the High-Altitude Cloud Physics Laboratory (HACPL: 17.56°N, 73.4°E, above 1373 m MSL), Mahabaleshwar were investigated for determining the diurnality of the drop size distribution (DSD) associated with the precipitation characteristics over the Western Ghats of India. The JWD data for the period from 2015 to 2019 were collected and examined during the Indian Summer Monsoon (ISM) season. The number concentration of rain droplets of various diameters is considerably varying with the rain rate (R) and type of precipitating cloud. With increasing the value of R, rain droplets having larger diameter concentration significantly increases, and the distribution tail moves towards the biggest droplets. The average value of reflectivity (Z), R, liquid water content (LWC), mass-weighted mean diameter (Dm), and normalized intercept parameter (log10Nw) was found to be higher for the heavy rainfall (Rhigh =10 mm h-1) as compared to the low rainfall (Rlow < 10 mm h-1) during the entire study period. The gamma distribution of DSD shows significant differences during the low and heavy precipitation on different time periods (e.g., 00-06, 06-12, 12-18, 18-23 LST). The number of rain events contributing to the total accumulated rain varies with time. The maximum number of rain events occurred during 12-18 LST, with 23.6 % rain events of low rainfall and 4.9% of heavy rainfall. The bimodality is observed in the diurnal variation of Dm, R, and Z, with the largest peak recorded in the late afternoon hour (13-16 LST) and the second crest in the early morning hour (05 LST). At the same time, the log10Nw value drops down, indicating the lowest concentration of rain droplets. [ABSTRACT FROM AUTHOR]

Accumulation of edible biomass by crop plants relies on maintenance of a high photosynthetic rates across the photoperiod, with assimilation rate (A) generally responding to increasing light intensity in a hyperbolic fashion. In natural environments light fluctuates greatly over the course of the day, however in Controlled Environmental Agricultural (CEA) systems, light intensity can be supplemented or precisely controlled using LEDs to create near optimum conditions. In such indoor growth environments light is often delivered as a square wave and recommendations to horticulturalists are given in the form of Daily Light Integrals (DLI). However, this does not take into account the slow photosynthetic induction at the start of the photoperiod and the decline of A towards the end of the photoperiod, which has been demonstrated by several previous studies. Square wave light regimes therefore potentially cause suboptimal photosynthetic utilization of the applied lighting and waste electricity. Here we have adapted light recipes to gradually increase and decrease in intensity to take account of these findings. We demonstrate that, utilising a sinusoidal light regime capped at 250 μmol m-2 s-1, it is possible to increase edible biomass of rocket (by ca. 20%) compared to square wave delivered at 250 at the same DLI. Additionally, this can be achieved using less electricity (0.6%), therefore reducing energy costs and improving profitability. We suggest that capping maximum light intensity at 250 µmol m-2 s-1 improves the operating efficiency of PSII photochemistry (Fq’/Fm’) also known as the photosynthetic efficiency by maintaining A later in the photoperiod. We show that a higher electron transfer rate (ETR) is maintained in these treatments over the photoperiod compared to higher light intensity caps, resulting in a greater Daily Photochemical Integral (DPI). We attribute this to less NPQ due to a greater sink capacity for the end products of electron transport, ATP and NADPH, as A is kept high for longer.

BACKGROUND: The Nile rat (Avicanthis niloticus) is an important animal model because of its robust diurnal rhythm, a cone-rich retina, and a propensity to develop diet-induced diabetes without chemical or genetic modifications. A closer similarity to humans in these aspects, compared to the widely used Mus musculus and Rattus norvegicus models, holds the promise of better translation of research findings to the clinic. RESULTS: We report a 2.5 Gb, chromosome-level reference genome assembly with fully resolved parental haplotypes, generated with the Vertebrate Genomes Project (VGP). The assembly is highly contiguous, with contig N50 of 11.1 Mb, scaffold N50 of 83 Mb, and 95.2% of the sequence assigned to chromosomes. We used a novel workflow to identify 3613 segmental duplications and quantify duplicated genes. Comparative analyses revealed unique genomic features of the Nile rat, including some that affect genes associated with type 2 diabetes and metabolic dysfunctions. We discuss 14 genes that are heterozygous in the Nile rat or highly diverged from the house mouse. CONCLUSIONS: Our findings reflect the exceptional level of genomic resolution present in this assembly, which will greatly expand the potential of the Nile rat as a model organism.

Brain tissue transcriptomes may be organized into gene coexpression networks, but their underlying biological drivers remain incompletely understood. Here, we undertook a large-scale transcriptomic study using 508 wild-type mouse striatal tissue samples dissected exclusively in the afternoons to define 38 highly reproducible gene coexpression modules. We found that 13 and 11 modules are enriched in cell-type and molecular complex markers, respectively. Importantly, 18 modules are highly enriched in daily rhythmically expressed genes that peak or trough with distinct temporal kinetics, revealing the underlying biology of striatal diurnal gene networks. Moreover, the diurnal coexpression networks are a dominant feature of daytime transcriptomes in the mouse cortex. We next employed the striatal coexpression modules to decipher the striatal transcriptomic signatures from Huntingtons disease models and heterozygous null mice for 52 genes, uncovering novel functions for Prkcq and Kdm4b in oligodendrocyte differentiation and bipolar disorder-associated Trank1 in regulating anxiety-like behaviors and nocturnal locomotion.

Given the marked variation in abiotic and biotic conditions between day and night, many species specialise their physical activity to being diurnal or nocturnal, and it was long thought that these strategies were commonly fairly fixed and invariant. The term 'cathemeral', was coined in 1987, when Tattersall noted activity in a Madagascan primate during the hours of both daylight and darkness. Initially thought to be rare, cathemerality is now known to be a quite widespread form of time partitioning amongst arthropods, fish, birds, and mammals. Herein we provide a synthesis of present understanding of cathemeral behaviour, arguing that it should routinely be included alongside diurnal and nocturnal strategies in schemes that distinguish and categorise species across taxa according to temporal niche. This synthesis is particularly timely because (i) the study of animal activity patterns is being revolutionised by new and improved technologies; (ii) it is becoming apparent that cathemerality covers a diverse range of obligate to facultative forms, each with their own common sets of functional traits, geographic ranges and evolutionary history; (iii) daytime and nighttime activity likely plays an important but currently neglected role in temporal niche partitioning and ecosystem functioning; and (iv) cathemerality may have an important role in the ability of species to adapt to human‐mediated pressures. [ABSTRACT FROM AUTHOR]

Introduction: Roses are one of the most essential ornamental flowers and are commonly used in perfumery, cosmetics, and food. They are rich in bioactive compounds, which are of interest for therapeutic effects. Objectives: The objective of this study was to understand the kinds of changes that occur between the nocturnal and diurnal metabolism of rose and to suggest hypotheses. Methods: Reversed-phase ultrahigh-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry or triple quadrupole mass spectrometry (TQ MS/MS) was used for nontargeted metabolomics and hormonal profiling respectively. For metabolite annotation, accurate mass spectra were compared with those in databases. Results: The hormonal profile of flowers showed an increase in jasmonate at night, while that of leaves indicated an increase in the salicylic acid pathway. Nontargeted analyses of the flower revealed a switch in the plant's defense mechanisms from glycosylated metabolites during the day to acid metabolites at night. In leaves, a significant decrease in flavonoids was observed at night in favor of acid metabolism to maintain a level of protection. Moreover, it might be possible to place back some of the annotated molecules on the shikimate pathway. Conclusion: The influence of day and night on the metabolome of rose flowers and leaves has been clearly demonstrated. The hormonal modulations occurring during the night and at day are consistent with the plant circadian cycle. A proposed management of the sesquiterpenoid and triterpenoid biosynthetic pathway may explain these changes in the flower. In leaves, the metabolic differences may reflect night-time regulation in favor of the salicylic acid pathway. [ABSTRACT FROM AUTHOR]

To quantify how representative a single measure of reproductive hormone level is of the daily hormonal profile using data from detailed hormonal sampling in the saline placebo-treated arm conducted over several hours. Retrospective analysis of data from previous interventional research studies evaluating reproductive hormones. Clinical Research Facility at a tertiary reproductive endocrinology centre at Imperial College Hospital NHS Foundation Trust. Overall, 266 individuals, including healthy men and women (n = 142) and those with reproductive disorders and states (n = 124 [11 with functional hypothalamic amenorrhoea, 6 with polycystic ovary syndrome, 62 women and 32 men with hypoactive sexual desire disorder, and 13 postmenopausal women]), were included in the analysis. Data from 266 individuals who had undergone detailed hormonal sampling in the saline placebo-treated arms of previous research studies was used to quantify the variability in reproductive hormones because of pulsatile secretion, diurnal variation, and feeding using coefficient of variation (CV) and entropy. The ability of a single measure of reproductive hormone level to quantify the variability in reproductive hormone levels because of pulsatile secretion, diurnal variation, and nutrient intake. The initial morning value of reproductive hormone levels was typically higher than the mean value throughout the day (percentage decrease from initial morning measure to daily mean: luteinizing hormone level 18.4%, follicle-stimulating hormone level 9.7%, testosterone level 9.2%, and estradiol level 2.1%). Luteinizing hormone level was the most variable (CV 28%), followed by sex-steroid hormone levels (testosterone level 12% and estradiol level 13%), whereas follicle-stimulating hormone level was the least variable reproductive hormone (CV 8%). In healthy men, testosterone levels fell between 9:00 am and 5:00 pm by 14.9% (95% confidence interval 4.2, 25.5%), although morning levels correlated with (and could be predicted from) late afternoon levels in the same individual (r 2 = 0.53, P <.0001). Testosterone levels were reduced more after a mixed meal (by 34.3%) than during ad libitum feeding (9.5%), after an oral glucose load (6.0%), or an intravenous glucose load (7.4%). Quantification of the variability of a single measure of reproductive hormone levels informs the reliability of reproductive hormone assessment. [ABSTRACT FROM AUTHOR]

How animals use the diel period (24‐h light–dark cycle) is of fundamental importance to understand their niche. While ecological and evolutionary literature abound with discussion of diel phenotypes (e.g. diurnal, nocturnal, crepuscular, cathemeral), they lack clear and explicit quantitative definitions. As such, inference can be confounded when evaluating hypotheses of animal diel niche switching or plasticity across studies because researchers may be operating under different definitions of diel phenotypes.We propose quantitative definitions of diel phenotypes using four alternative hypothesis sets (maximizing, traditional, general and selection) aimed at achieving different objectives. Each hypothesis set is composed of mutually exclusive hypotheses defined based on the activity probabilities in the three fundamental periods of light availability (twilight, daytime and night‐time).We develop a Bayesian modelling framework that compares diel phenotype hypotheses using Bayes factors and estimates model parameters using a multinomial model with linear inequality constraints. Model comparison, parameter estimation and visualizing results can be done in the Diel.Niche R package. A simplified R Shiny web application is also available.We provide extensive simulation results to guide researchers on the power to discriminate among hypotheses for a range of sample sizes (10–1280). We also work through several examples of using data to make inferences on diel activity, and include online vignettes on how to use the Diel.Niche package. We demonstrate how our modelling framework complements other analyses, such as circular kernel density estimators and animal movement modelling.Our aim is to encourage standardization of the language of diel activity and bridge conceptual frameworks and hypotheses in diel research with data and models. Lastly, we hope more research focuses on the ecological and conservation importance of understanding how animals use diel time. [ABSTRACT FROM AUTHOR]

Pesticide exposure can be harmful to insect pollinators and the ecosystem services they provide. As many pesticide guidelines warn against applying such products when pollinators are active, it is important to evaluate how pollinator activity changes with time of day to determine the most appropriate time to spray.We walked transects from sunrise to sunset in oilseed rape (Brassica napus L.) fields in Ireland to capture the abundance of honeybees, bumblebees, solitary bees, and hoverflies across daylight hours. We also recorded the activity of representative species from the three bee groups at their nests across similar time periods to compare with field observations.Peak pollinator abundance was in the mid-afternoon with fewer individuals in the early morning and late evening for all groups. At the nest we observed patterns of activity that broadly reflected field abundance but indicated that bees are active earlier and later than observed on the crop. However, there were differences between pollinator groups. Overall, honeybee and solitary bee abundance and activity were found to peak in the middle of the day, while bumblebee abundance and activity was more consistent throughout daylight hours. Hoverflies were relatively abundant in the morning and increased in number towards the late afternoon and early evening.Our results confirm current recommendations that pesticide application should be avoided in the middle of the day when pollinators are most active. However, the diversity of responses within and between pollinator groups to time of day should be accounted for when shaping guidelines, and clearly defining optimal pesticide application timings for end users is difficult and needs further consideration as it will vary between regions and crops. Further research should also explore how time impacts both pesticide efficacy and exposure of pollinators to residues post-application to allow full evaluation of how practical and beneficial timing of application may be when aiming to protect pollinators from pesticide exposure.

Housing conditions are essential for ensuring animal welfare and high-quality research outcomes. In this study, we continuously monitored air quality—specifically ammonia, carbon dioxide, relative humidity, and temperature—in Individually Ventilated Cages (IVCs) housing five female or male C57BL/6N mice. The cages were cleaned either weekly or bi-weekly, and the data were collected as the mice aged from 100 to 348 days. The survival rate remained above 96%, with body weight increasing by 35–52% during the study period. The ammonia levels rose throughout the cleaning cycle, but averaged below 25 ppm. However, in the older, heavier mice with bi-weekly cage cleaning, the ammonia levels reached between 25 and 75 ppm, particularly in the males. While circadian rhythms influenced the ammonia concentration only to a small extent, the carbon dioxide levels varied between 800 and 3000 ppm, increasing by 30–50% at night and by 1000 ppm with body weight. Humidity also correlated primarily with the circadian rhythms (10% higher at night) and, to a lesser extent, with body weight, reaching ≥70% in the middle-aged mice. The temperature variations remained minimal, within a 1 °C range. We conclude that air quality assessments in IVCs should be conducted during animals’ active periods, and both housing density and biomass must be considered to optimise welfare.

A pasture or concentrate-based dietary regime impacts a variety of factors including both ruminal health and function, and consequently milk production and quality. The objective of this study was to examine the effect of feeding differing pasture levels on the metabolite composition of bovine ruminal fluid. Ruminal fluid was obtained from rumen-cannulated spring-calving cows (N = 9, Holstein-Friesian breed, average lactation number = 5) fed one of three diets across a full lactation season. Group 1 (pasture) consumed perennial ryegrass supplemented with 5% concentrates; group 2 received a total mixed ration (TMR) diet; and group 3 received a partial mixed ration (PMR) diet which included pasture and a TMR. Samples were taken at two timepoints: morning and evening. Metabolomic analysis was performed using nuclear magnetic resonance (1H-NMR) spectroscopy. Statistical analysis revealed significant changes across the dietary regimes in both morning and evening samples, with distinct alterations in the metabolite composition of ruminal fluid from pasture-fed cows (FDR-adjusted p-value < 0.05). Acetate and butyrate were significantly higher in samples derived from a pasture-based diet whereas sugar-related metabolites were higher in concentrate-based samples. Furthermore, a distinct diurnal impact on the metabolite profile was evident. This work lays the foundation for understanding the complex interaction between dietary regime and ruminal health.

Aim: Brain volume is influenced by several factors that can change throughout the day. In addition, most of these factors are influenced by sleep quality. This study investigated diurnal variation in brain volume and its relation to overnight sleep quality. Methods: We enrolled 1,003 healthy Koreans without any psychiatric disorders aged 60 years or older. We assessed sleep quality and average wake time using the Pittsburgh Sleep Quality Index, and divided sleep quality into good, moderate, and poor groups. We estimated the whole and regional brain volumes from three-dimensional T1-weighted brain MRI scans. We divided the interval between average wake-up time and MRI acquisition time (INT) into tertile groups: short (INT1), medium (INT2), and long (INT3). Results: Whole and regional brain volumes showed no significance with respect to INT. However, the `interaction between INT and sleep quality showed significance for whole brain, cerebral gray matter, and cerebrospinal fluid volumes (p < .05). The INT2 group showed significantly lower volumes of whole brain, whole gray matter, cerebral gray matter, cortical gray matter, subcortical gray matter, and cerebrospinal fluid than the INT1 and INT3 groups only in the individuals with good sleep quality. Conclusion: Human brain volume changes significantly within a day associated with overnight sleep in the individuals with good sleep quality.

Environmental differences have fostered the evolution of ecological processes and functions that are unique to the nighttime but intertwined with those occurring during the daytime. However, relative to the daytime, research at night has long been neglected. Growing realisation of this strong diurnal research bias combined with technological advances has begun to galvanise interest in how physiological processes, species interactions, communities, and thence ecosystem functions are structured across the diel cycle. In part, the importance of diel variation in ecosystem functioning is also being revealed by anthropogenic pressures that disproportionately act during the daytime or nighttime. Accounting for day–night variation promises to provide deeper insights into not only how ecosystems function but also how functions respond to human-mediated disturbances. Given the marked differences in environmental conditions and active biota between daytime and nighttime, it is almost inevitable that ecosystem functioning will also differ. However, understanding of these differences has been hampered due to the challenges of conducting research at night. At the same time, many anthropogenic pressures are most forcefully exerted or have greatest effect during either daytime (e.g., high temperatures, disturbance) or nighttime (e.g., artificial lighting, nights warming faster than days). Here, we explore current understanding of diel (daily) variation in five key ecosystem functions and when during the diel cycle they primarily occur [predation (unclear), herbivory (nighttime), pollination (daytime), seed dispersal (unclear), carbon assimilation (daytime)] and how diel asymmetry in anthropogenic pressures impacts these functions. [ABSTRACT FROM AUTHOR]

Background: Direct fuel moisture content measurements are critical for characterising spatio-temporal variations in fuel flammability and for informing fire danger assessments. However, among-sampler variability (systematic differences in measurements between samplers) likely contributes to fuel moisture measurement variability in most field campaigns. Aims: We assessed the magnitude of among-sampler variability in plot-scale Calluna vulgaris fuel moisture measurements. Methods: Seventeen individuals collected samples from six fuel layers hourly from 10:00 hours to 18:00 hours. We developed mixed effects models to estimate the among-sampler variability. Key results: Fuel moisture measurements were highly variable between individuals sampling within the same plot, fuel layer, and time of day. The importance of among-sampler variability in explaining total measured fuel moisture variance was fuel layer dependent. Among-sampler variability explained the greatest amount of measurement variation in litter (58%) and moss (45%) and was more important for live (19%) than dead (4%) Calluna. Conclusions: Both consideration of samplers within the experimental design and incorporation of sampler metadata during statistical analysis will improve understanding of spatio-temporal fuel moisture dynamics obtained from field-based studies. Implications: Accounting for among-sampler variability in fuel moisture campaigns opens opportunities to utilise sampling teams and citizen science research to examine fuel moisture dynamics over large spatio-temporal scales. Citizen science provides an important opportunity for the wildfire community, enabling data collection at broad spatio-temporal scales. Direct fuel moisture measurement campaigns can maximise these benefits by accounting for natural differences in measurements between individuals. We quantify the magnitude and variability of differences during an intensive fuel moisture measurement campaign. [ABSTRACT FROM AUTHOR]

Biological rhythms are repeating patterns that are driven by time-keeping mechanisms within the animal and are adaptive as they coordinate physiology and metabolism with the external environment. The dairy cow has a well recognised natural daily pattern of feed intake and milk synthesis, but regulation of these rhythms has not been well described in the literature or well considered in current dairy management. Recent discoveries have clearly described circadian time-keeping mechanisms in peripheral tissues that are responsive to the timing of food availability. Some management strategies on dairy farms may desynchronise the interactions between central and mammary circadian timekeepers, resulting in reduced milk yield and efficiency. Feeding a total mixed ration is commonly assumed to create constant ruminal conditions, but the large variation in the rate of feed intake across the day causes large fluctuations in rumen fermentation and absorbed nutrients. Milk composition also differs across the day due to both dynamics in nutrient absorption and biological regulation attempting to match milk yield and composition with calf requirements across the day. Recent work has shown that milk synthesis varies over the day and is modified by the timing of feed intake and nutrient absorption. These rhythms have also been shown to be affected by the timing of feed delivery. We expect that maximal milk yield and efficiency are achieved when we have more consistent rumen fermentation and match the timing of nutrient absorption and mammary capacity for milk synthesis. Managing feeding times provides the opportunity to modify feed intake across the day, but behavioural responses are complex. Appreciating the impact of circadian rhythms provides the foundation to develop nutrition and management strategies considering circadian dynamics of intake and milk synthesis and provides opportunities for new gains in cow efficiency, welfare, and health. The dairy cow has a well recognised natural daily pattern of feed intake and milk synthesis that have implications for optimising rumen function and milk synthesis. Basic biology work has described the time-keeping mechanisms regulating these rhythms. The daily pattern of feed intake and the implications for rumen function and recent work showing that milk synthesis varies over the day and is modified by the timing of feed intake are discussed. [ABSTRACT FROM AUTHOR]

Knowledge of elephant movement and grouping patterns in the wild is critical for their management and conservation. Much of these data come from GPS collar data and aerial surveys, which have provided invaluable information, but data from these methods are often limited to small groups or entire populations. Effective elephant management requires both generalized and localized methodologies. Here, we propose the expanded use of camera traps in research relating to elephant localized movements and grouping patterns as an additional tool for elephant conservation management. In this study, we use a battery-powered camera trap to provide daily high-resolution data of African savanna elephant (Loxodonta africana) grouping patterns over the course of an entire year. We present findings on the seasonal and diurnal grouping patterns of elephants at a waterhole in the northeast corner of Etosha National Park from July 2016 to June 2017. The frequency of elephant occurrences varied seasonally and diurnally across all group types (solitary male, male, family, and mixed groups), while group sizes did not vary seasonally, except for male groups. Solitary males occurred relatively equally throughout the day, while male and mixed groups occurred the most midday, and family groups occurred the most in the afternoon. Additionally, we measured the reliability of research assistants when collecting group type and group size data from the camera trap images. Intra- and inter-observer reliability was excellent among and across research assistants, highlighting the potential for non-specialist observers to have greater involvement in camera trap data collection. Our results support the use of camera trap data where GPS collars and aerial surveys are not feasible and where higher-resolution data are needed for more localized management. Finally, we discuss our experience with two different types of camera traps to highlight the pros and cons of each approach. [ABSTRACT FROM AUTHOR]

Pesticide exposure can be harmful to insect pollinators and the ecosystem services they provide. As many pesticide guidelines warn against applying such products when pollinators are active, it is important to evaluate how pollinator activity changes with time of day to determine the most appropriate time to spray. We walked transects from sunrise to sunset in oilseed rape (Brassica napus L.) fields in Ireland to capture the abundance of honeybees, bumblebees, solitary bees, and hoverflies across daylight hours. We also recorded the activity of representative species from the three bee groups at their nests across similar time periods to compare with field observations. Peak pollinator abundance was in the mid-afternoon with fewer individuals in the early morning and late evening for all groups. At the nest we observed patterns of activity that broadly reflected field abundance but indicated that bees are active earlier and later than observed on the crop. However, there were differences between pollinator groups. Overall, honeybee and solitary bee abundance and activity were found to peak in the middle of the day, while bumblebee abundance and activity was more consistent throughout daylight hours. Hoverflies were relatively abundant in the morning and increased in number towards the late afternoon and early evening. Our results confirm current recommendations that pesticide application should be avoided in the middle of the day when pollinators are most active. However, the diversity of responses within and between pollinator groups to time of day should be accounted for when shaping guidelines, and clearly defining optimal pesticide application timings for end users is difficult and needs further consideration as it will vary between regions and crops. Further research should also explore how time impacts both pesticide efficacy and exposure of pollinators to residues post-application to allow full evaluation of how practical and beneficial timing of application may be when aiming to protect pollinators from pesticide exposure. [ABSTRACT FROM AUTHOR]

IntroductionIncidents of myocardial infarction and sudden cardiac arrest vary with time of the day, but the mechanism for this effect is not clear. We hypothesized that diurnal changes in the ability of cardiac mitochondria to control calcium homeostasis dictate vulnerability to cardiovascular events.ObjectivesHere we investigate mitochondrial calcium dynamics, respiratory function, and reactive oxygen species (ROS) production in mouse heart during different phases of wake versus sleep periods.MethodsWe assessed time-of-the-day dependence of calcium retention capacity of isolated heart mitochondria from young male C57BL6 mice. Rhythmicity of mitochondrial-dependent oxygen consumption, ROS production and transmembrane potential in homogenates were explored using the Oroboros O2k Station equipped with a fluorescence detection module. Changes in expression of essential clock and calcium dynamics genes/proteins were also determined at sleep versus wake time points.ResultsOur results demonstrate that cardiac mitochondria exhibit higher calcium retention capacity and higher rates of calcium uptake during sleep period. This was associated with higher expression of clock gene Bmal1, lower expression of per2, greater expression of MICU1 gene (mitochondrial calcium uptake 1), and lower expression of the mitochondrial transition pore regulator gene cyclophilin D. Protein levels of mitochondrial calcium uniporter (MCU), MICU2, and sodium/calcium exchanger (NCLX) were also higher at sleep onset relative to wake period. While complex I and II-dependent oxygen utilization and transmembrane potential of cardiac mitochondria were lower during sleep, ROS production was increased presumably due to mitochondrial calcium sequestration.ConclusionsTaken together, our results indicate that retaining mitochondrial calcium in the heart during sleep dissipates membrane potential, slows respiratory activities, and increases ROS levels, which may contribute to increased vulnerability to cardiac stress during sleep-wake transition. This pronounced daily oscillations in mitochondrial functions pertaining to stress vulnerability may at least in part explain diurnal prevalence of cardiac pathologies.

Repeated blood sampling is required in certain clinical and research settings, which is currently performed by drawing blood from venous catheters requiring manual handling of each sample at the time of collection. A novel body-worn device for repeated serial samples, Fluispotter®, with automated extraction, collection, and storage of up to 20 venous dried blood spot samples over the course of 20 h may overcome problems with current methods for serial sampling. The purpose of this study was to assess the performance and safety of Fluispotter for the first time in healthy subjects. Fluispotter consists of a cartridge with tubing, a reservoir for flushing solution, pumps and filter paper, and a multi-lumen catheter placed in the brachial vein. We recruited healthy subjects for testing in an in-hospital setting. Fluispotter was attached by an anesthesiologist to 22 healthy subjects of which 9/22 (40.9%) participants had all 20 samples taken, which was lower than the goal of complete sampling in 80% of the subjects (P = 0.02). The main reason for sample failure was clogging of blood flow which was observed in 11/22 (50%) of the participants. No serious adverse events occurred, and the participants rated the pain from the insertion and the removal of catheter as very low. A cortisol profile showed nadir values at midnight and highest values at 05:00 h. Although full sampling was not successful in all participants, the Fluispotter technology proved safe and highly acceptable to the participants producing the expected cortisol profile without the requirement of staff during sample collection.

Abstract Background Current recommendations for physical exercise include information about the frequency, intensity, type, and duration of exercise. However, to date, there are no recommendations on what time of day one should exercise. The aim was to perform a systematic review with meta-analysis to investigate if the time of day of exercise training in intervention studies influences the degree of improvements in physical performance or health-related outcomes. Methods The databases EMBASE, PubMed, Cochrane Library, and SPORTDiscus were searched from inception to January 2023. Eligibility criteria were that the studies conducted structured endurance and/or strength training with a minimum of two exercise sessions per week for at least 2 weeks and compared exercise training between at least two different times of the day using a randomized crossover or parallel group design. Results From 14,125 screened articles, 26 articles were included in the systematic review of which seven were also included in the meta-analyses. Both the qualitative synthesis and the quantitative synthesis (i.e., meta-analysis) provide little evidence for or against the hypothesis that training at a specific time of day leads to more improvements in performance-related or health-related outcomes compared to other times. There was some evidence that there is a benefit when training and testing occur at the same time of day, mainly for performance-related outcomes. Overall, the risk of bias in most studies was high. Conclusions The current state of research provides evidence neither for nor against a specific time of the day being more beneficial, but provides evidence for larger effects when there is congruency between training and testing times. This review provides recommendations to improve the design and execution of future studies on this topic. Registration: PROSPERO (CRD42021246468).

Accurate and current rainfall analysis is crucial for planning and development in the new capital city of Indonesia (IKN). This study examines the effect of the Madden-Julian Oscillation (MJO) on rainfall variability in IKN using a 20-year dataset of Integrated Multi-Satellite Retrievals for GPM (IMERG) version 6 and automatic weather station (AWS) data. The study analyzes flood events in IKN by examining flood information provided by the National Agency for Disaster Countermeasures (BNPB) from 2008 to 2022 and the contribution of the MJO to these events. The findings indicate that the MJO significantly influences rainfall variability in IKN, with a more pronounced effect during the dry season (JJASO) than in the wet season (NDJFMAM). This is demonstrated by the higher occurrence of wet days and increased daily rainfall intensity, which is linked to extreme rainfall and longer-duration events, mainly between midnight and morning (0400–0600 LST). Notably, although floods are more common during the rainy season, the MJO can intensify flood events in IKN during the dry season. Therefore, when developing an effective flood disaster mitigation system for IKN, it is crucial to consider the amplitude and phase of the MJO.

Prader-Willi Syndrome (PWS) is a rare neurodevelopmental disorder that affects approximately 1 in 20,000 individuals worldwide. Symptom progression in PWS is classically characterized by two nutritional stages. Stage 1 is hypotonia characterized by poor muscle tone that leads to poor feeding behavior causing failure to thrive in early neonatal life. Stage 2 is followed by the development of extreme hyperphagia, also known as insatiable eating and fixation on food that often leads to obesity in early childhood. Other major features of PWS include obsessive-compulsive and hoarding behaviors, intellectual disability, and sleep abnormalities. PWS is genetic disorder mapping to imprinted 15q11.2-q13.3 locus, specifically at the paternally expressed SNORD116 locus of small nucleolar RNAs and noncoding host gene transcripts. SNORD116 is processed into several noncoding components and is hypothesized to orchestrate diurnal changes in metabolism through epigenetics, according to functional studies. Here, we review the current status of epigenetic mechanisms in PWS, with an emphasis on an emerging role for SNORD116 in circadian and sleep phenotypes. We also summarize current ongoing therapeutic strategies, as well as potential implications for more common human metabolic and psychiatric disorders.

Octopus insularis is a benthic octopod from the tropical and subtropical Western Atlantic Ocean that inhabits semi‐permanent "dens" (small crevices) in hard substrate. We used visual surveys to assess den occupancy and remote cameras at den entrances to assess activity patterns of O. insularis of South Caicos (21.5112° N, 71.5190° W) in the Turks and Caicos Islands (May–August, 2020 and July–December, 2021). Dens were occupied for a median of 4 days, but occupancy ranged widely (1 day–2 months), indicating migration between dens at erratic intervals. The two most prevalent behaviors were Away from the Den, time presumably spent mostly foraging, and Quiescence, when the octopus was sleeping or otherwise unreactive. Our data indicate that Octopus insularis is diurnal, with time Away from the Den peaking between mid‐morning (0700–1000) and late afternoon (1600–2000), and with Quiescence peaking at night (2000–0600) and also in the middle of the day (1100–1600), although adherence to this pattern was not strict. High interindividual variation and high within‐individual stability in the proportions of time Away from the Den and Quiescent suggest that individuals of this species vary greatly from each other in their hunting and resting patterns, while also showing high levels of internal consistency for at least a week. These observations will guide future research with this commercially important species, further differentiates O. insularis from congeners, and demonstrate the efficacy of minimally disruptive field techniques in studying behavior. [ABSTRACT FROM AUTHOR]

The rise of urbanization has led to many critical issues like increased air pollution, emission of more greenhouse gases, sudden climate change, and a rise in temperature of an urban area compared to surrounding rural regions known as urban heat islands (UHI). It becomes crucial to understand the effect of climatic conditions in those areas. In this present research, we have chosen two different cities: Bhopal and Guwahati, situated over different climatic conditions. The study examines the diurnal, seasonal and inter annual variability of surface UHI (SUHI), using MODIS LST (MOD11A2 and MYD11A2) product of 1 km spatial resolution from 2003 to 2018. The temporal trend of LST is estimated using Mann–Kendall and Sen's slope estimator test. This study also evaluates the land use land cover (LULC) change over the two cities using the LULC data. The present research also incorporates the determination of six different driving factors of SUHI starting from built-up area, urban–rural difference in evapotranspiration, black sky albedo, enhanced vegetation index, thermal inertia, and population. Pearson's correlation criteria are used to determine the correlation of SUHI with each of the determinant parameters. The results reveal the presence of a cool urban island during the summer daytime in Bhopal, while Guwahati shows a positive SUHI. The nighttime shows clear evidence and higher SUHI magnitude than the daytime for Bhopal city. Contrary, a reverse phenomenon is evident in Guwahati with higher SUHI magnitude during the daytime compared to nighttime. The negative SUHI variation can be ascribed to rural area dynamics with the majority of cropland cover. The evapotranspiration shows reasonable control of SUHI's daytime variation. Thermal inertia was found to be an influential parameter in explaining the diurnal variation of SUHI. [ABSTRACT FROM AUTHOR]