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Your search keyword '"Devi, Aribam Swarmistha"' showing total 5 results
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5. Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the collagen-binding region of RspB from Erysipelothrix rhusiopathiae.

Author

Devi, Aribam Swarmistha, Ogawa, Yohsuke, Shimoji, Yoshihiro, and Ponnuraj, Karthe

Subjects
*ERYSIPELOTHRIX rhusiopathiae, *COLLAGEN-binding proteins, *SELENOMETHIONINE, *GEL permeation chromatography, *CRYSTALS, *SPACE groups
Abstract

RspB is a surface adhesin of Erysipelothrix rhusiopathiae. A recombinant form of the collagen-binding region of this protein, RspB(31-348), has been overexpressed in Escherichia coli in native and selenomethionine-derivative forms and purified using affinity and gel-permeation chromatography. Thin plate-like crystals were obtained by the hanging-drop vapour-diffusion method using the same condition for both forms. The native crystals diffracted to a resolution of 2.5 Å using an in-house X-ray source, while the selenomethionine-derivative crystals diffracted to a resolution of 2.2 Å using synchrotron radiation. The crystals belonged to the monoclinic space group P21, with unit-cell parameters a = 46.19, b = 66.65, c = 101.72 Å, β = 94.11°. [ABSTRACT FROM AUTHOR]

Published
2010
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