201 results on '"Corre E"'
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2. FAKE NEWS COMO FERRAMENTA DE ATAQUE À DEMOCRACIA
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CORRE, E. L. B., primary
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- 2023
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3. Oyster hemolymph is a complex and dynamic ecosystem hosting bacteria, protists and viruses
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Dupont, S., Lokmer, A., Corre, E., Auguet, J.-C., Petton, B., Toulza, E., Montagnani, C., Tanguy, G., Pecqueur, D., Salmeron, C., Guillou, L., Desnues, C., La Scola, B., Bou Khalil, J., de Lorgeril, J., Mitta, G., Gueguen, Y., and Escoubas, J.-M.
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- 2020
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4. Differential global distribution of marine picocyanobacteria gene clusters reveals distinct niche-related adaptive strategies.
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Doré, H, Guyet, U, Leconte, J, Farrant, GK, Alric, B, Ratin, M, Ostrowski, M, Ferrieux, M, Brillet-Guéguen, L, Hoebeke, M, Siltanen, J, Le Corguillé, G, Corre, E, Wincker, P, Scanlan, DJ, Eveillard, D, Partensky, F, Garczarek, L, Doré, H, Guyet, U, Leconte, J, Farrant, GK, Alric, B, Ratin, M, Ostrowski, M, Ferrieux, M, Brillet-Guéguen, L, Hoebeke, M, Siltanen, J, Le Corguillé, G, Corre, E, Wincker, P, Scanlan, DJ, Eveillard, D, Partensky, F, and Garczarek, L
- Abstract
The ever-increasing number of available microbial genomes and metagenomes provides new opportunities to investigate the links between niche partitioning and genome evolution in the ocean, especially for the abundant and ubiquitous marine picocyanobacteria Prochlorococcus and Synechococcus. Here, by combining metagenome analyses of the Tara Oceans dataset with comparative genomics, including phyletic patterns and genomic context of individual genes from 256 reference genomes, we show that picocyanobacterial communities thriving in different niches possess distinct gene repertoires. We also identify clusters of adjacent genes that display specific distribution patterns in the field (eCAGs) and are thus potentially involved in the same metabolic pathway and may have a key role in niche adaptation. Several eCAGs are likely involved in the uptake or incorporation of complex organic forms of nutrients, such as guanidine, cyanate, cyanide, pyrimidine, or phosphonates, which might be either directly used by cells, for example for the biosynthesis of proteins or DNA, or degraded to inorganic nitrogen and/or phosphorus forms. We also highlight the enrichment of eCAGs involved in polysaccharide capsule biosynthesis in Synechococcus populations thriving in both nitrogen- and phosphorus-depleted areas vs. low-iron (Fe) regions, suggesting that the complexes they encode may be too energy-consuming for picocyanobacteria thriving in the latter areas. In contrast, Prochlorococcus populations thriving in Fe-depleted areas specifically possess an alternative respiratory terminal oxidase, potentially involved in the reduction of Fe(III) to Fe(II). Altogether, this study provides insights into how phytoplankton communities populate oceanic ecosystems, which is relevant to understanding their capacity to respond to ongoing climate change.
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- 2023
5. Global Phylogeography of Marine Synechococcus in Coastal Areas Reveals Strong Community Shifts.
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Blanchard, JL, Doré, H, Leconte, J, Guyet, U, Breton, S, Farrant, GK, Demory, D, Ratin, M, Hoebeke, M, Corre, E, Pitt, FD, Ostrowski, M, Scanlan, DJ, Partensky, F, Six, C, Garczarek, L, Blanchard, JL, Doré, H, Leconte, J, Guyet, U, Breton, S, Farrant, GK, Demory, D, Ratin, M, Hoebeke, M, Corre, E, Pitt, FD, Ostrowski, M, Scanlan, DJ, Partensky, F, Six, C, and Garczarek, L
- Abstract
Marine Synechococcus comprise a numerically and ecologically prominent phytoplankton group, playing a major role in both carbon cycling and trophic networks in all oceanic regions except in the polar oceans. Despite their high abundance in coastal areas, our knowledge of Synechococcus communities in these environments is based on only a few local studies. Here, we use the global metagenome data set of the Ocean Sampling Day (June 21st, 2014) to get a snapshot of the taxonomic composition of coastal Synechococcus communities worldwide, by recruitment on a reference database of 141 picocyanobacterial genomes, representative of the whole Prochlorococcus, Synechococcus, and Cyanobium diversity. This allowed us to unravel drastic community shifts over small to medium scale gradients of environmental factors, in particular along European coasts. The combined analysis of the phylogeography of natural populations and the thermophysiological characterization of eight strains, representative of the four major Synechococcus lineages (clades I to IV), also brought novel insights about the differential niche partitioning of clades I and IV, which most often co-dominate the Synechococcus community in cold and temperate coastal areas. Altogether, this study reveals several important characteristics and specificities of the coastal communities of Synechococcus worldwide. IMPORTANCE Synechococcus is the second most abundant phytoplanktonic organism on Earth, and its wide genetic diversity allowed it to colonize all the oceans except for polar waters, with different clades colonizing distinct oceanic niches. In recent years, the use of global metagenomics data sets has greatly improved our knowledge of "who is where" by describing the distribution of Synechococcus clades or ecotypes in the open ocean. However, little is known about the global distribution of Synechococcus ecotypes in coastal areas, where Synechococcus is often the dominant phytoplanktonic organism. Here, we leverage
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- 2022
6. Deciphering the infectious process of Colletotrichum lupini in lupin through transcriptomic and proteomic analysis
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Dubrulle G., Picot A., Madec S., Corre E., Pawtowski A., Baroncelli R., Zivy M., Balliau T., Le Floch G., Pensec F., Dubrulle G., Picot A., Madec S., Corre E., Pawtowski A., Baroncelli R., Zivy M., Balliau T., Le Floch G., and Pensec F.
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Anthracnose disease ,Pathogenicity factor ,Proteome ,Transcriptome - Abstract
The fungal phytopathogen Colletotrichum lupini is responsible for lupin anthracnose, resulting in significant yield losses worldwide. The molecular mechanisms underlying this infectious process are yet to be elucidated. This study proposes to evaluate C. lupini gene expression and protein synthesis during lupin infection, using, respectively, an RNAseq-based transcriptomic approach and a mass spectrometry-based proteomic approach. Patterns of differentially-expressed genes in planta were evaluated from 24 to 84 hours post-inoculation, and compared to in vitro cultures. A total of 897 differentially-expressed genes were identified from C. lupini during interaction with white lupin, of which 520 genes were predicted to have a putative function, including carbohydrate active enzyme, effector, protease or transporter-encoding genes, commonly described as pathogenicity factors for other Colletotrichum species during plant infection, and 377 hypothetical proteins. Simultaneously, a total of 304 proteins produced during the interaction were identified and quantified by mass spectrometry. Taken together, the results highlight that the dynamics of symptoms, gene expression and protein synthesis shared similarities to those of hemibiotrophic pathogens. In addition, a few genes with unknown or poorly-described functions were found to be specifically associated with the early or late stages of infection, suggesting that they may be of importance for pathogenicity. This study, conducted for the first time on a species belonging to the Colletotrichum acutatum species complex, presents an opportunity to deepen functional analyses of the genes involved in the pathogenicity of Colletotrichum spp. during the onset of plant infection.
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- 2020
7. Oyster hemolymph is a complex and dynamic ecosystem hosting bacteria, protists and viruses
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Dupont, Samuel, Lokmer, A., Corre, E., Auguet, Jean-christophe, Petton, Bruno, Toulza, E., Montagnani, Caroline, Tanguy, G., Pecqueur, D., Salmeron, C., Guillou, L., Desnues, C., La Scola, B., Bou Khalil, J., De Lorgeril, Julien, Mitta, Guillaume, Gueguen, Yannick, Escoubas, Jean Michel, Dupont, Samuel, Lokmer, A., Corre, E., Auguet, Jean-christophe, Petton, Bruno, Toulza, E., Montagnani, Caroline, Tanguy, G., Pecqueur, D., Salmeron, C., Guillou, L., Desnues, C., La Scola, B., Bou Khalil, J., De Lorgeril, Julien, Mitta, Guillaume, Gueguen, Yannick, and Escoubas, Jean Michel
- Abstract
Background The impact of the microbiota on host fitness has so far mainly been demonstrated for the bacterial microbiome. We know much less about host-associated protist and viral communities, largely due to technical issues. However, all microorganisms within a microbiome potentially interact with each other as well as with the host and the environment, therefore likely affecting the host health. Results We set out to explore how environmental and host factors shape the composition and diversity of bacterial, protist and viral microbial communities in the Pacific oyster hemolymph, both in health and disease. To do so, five oyster families differing in susceptibility to the Pacific oyster mortality syndrome were reared in hatchery and transplanted into a natural environment either before or during a disease outbreak. Using metabarcoding and shotgun metagenomics, we demonstrate that hemolymph can be considered as an ecological niche hosting bacterial, protist and viral communities, each of them shaped by different factors and distinct from the corresponding communities in the surrounding seawater. Overall, we found that hemolymph microbiota is more strongly shaped by the environment than by host genetic background. Co-occurrence network analyses suggest a disruption of the microbial network after transplantation into natural environment during both non-infectious and infectious periods. Whereas we could not identify a common microbial community signature for healthy animals, OsHV-1 μVar virus dominated the hemolymph virome during the disease outbreak, without significant modifications of other microbiota components. Conclusion Our study shows that oyster hemolymph is a complex ecosystem containing diverse bacteria, protists and viruses, whose composition and dynamics are primarily determined by the environment. However, all of these are also shaped by oyster genetic backgrounds, indicating they indeed interact with the oyster host and are therefore not only of transi
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- 2020
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8. Cyanorak v2.1: a scalable information system dedicated to the visualization and expert curation of marine and brackish picocyanobacteria genomes.
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Garczarek L, Guyet U, Doré H, Farrant GK, Hoebeke M, Brillet-Guéguen L, Bisch A, Ferrieux M, Siltanen J, Corre E, Le Corguillé G, Ratin M, Pitt FD, Ostrowski M, Conan M, Siegel A, Labadie K, Aury J-M, Wincker P, Scanlan DJ, Partensky F, Garczarek L, Guyet U, Doré H, Farrant GK, Hoebeke M, Brillet-Guéguen L, Bisch A, Ferrieux M, Siltanen J, Corre E, Le Corguillé G, Ratin M, Pitt FD, Ostrowski M, Conan M, Siegel A, Labadie K, Aury J-M, Wincker P, Scanlan DJ, and Partensky F
- Abstract
Cyanorak v2.1 (http://www.sb-roscoff.fr/cyanorak) is an information system dedicated to visualizing, comparing and curating the genomes of Prochlorococcus, Synechococcus and Cyanobium, the most abundant photosynthetic microorganisms on Earth. The database encompasses sequences from 97 genomes, covering most of the wide genetic diversity known so far within these groups, and which were split into 25,834 clusters of likely orthologous groups (CLOGs). The user interface gives access to genomic characteristics, accession numbers as well as an interactive map showing strain isolation sites. The main entry to the database is through search for a term (gene name, product, etc.), resulting in a list of CLOGs and individual genes. Each CLOG benefits from a rich functional annotation including EggNOG, EC/K numbers, GO terms, TIGR Roles, custom-designed Cyanorak Roles as well as several protein motif predictions. Cyanorak also displays a phyletic profile, indicating the genotype and pigment type for each CLOG, and a genome viewer (Jbrowse) to visualize additional data on each genome such as predicted operons, genomic islands or transcriptomic data, when available. This information system also includes a BLAST search tool, comparative genomic context as well as various data export options. Altogether, Cyanorak v2.1 constitutes an invaluable, scalable tool for comparative genomics of ecologically relevant marine microorganisms.
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- 2020
9. Evolutionary Mechanisms of Long-Term Genome Diversification Associated With Niche Partitioning in Marine Picocyanobacteria.
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Doré H, Farrant GK, Guyet U, Haguait J, Humily F, Ratin M, Pitt FD, Ostrowski M, Six C, Brillet-Guéguen L, Hoebeke M, Bisch A, Le Corguillé G, Corre E, Labadie K, Aury J-M, Wincker P, Choi DH, Noh JH, Eveillard D, Scanlan DJ, Partensky F, Garczarek L, Doré H, Farrant GK, Guyet U, Haguait J, Humily F, Ratin M, Pitt FD, Ostrowski M, Six C, Brillet-Guéguen L, Hoebeke M, Bisch A, Le Corguillé G, Corre E, Labadie K, Aury J-M, Wincker P, Choi DH, Noh JH, Eveillard D, Scanlan DJ, Partensky F, and Garczarek L
- Abstract
Marine picocyanobacteria of the genera Prochlorococcus and Synechococcus are the most abundant photosynthetic organisms on Earth, an ecological success thought to be linked to the differential partitioning of distinct ecotypes into specific ecological niches. However, the underlying processes that governed the diversification of these microorganisms and the appearance of niche-related phenotypic traits are just starting to be elucidated. Here, by comparing 81 genomes, including 34 new Synechococcus, we explored the evolutionary processes that shaped the genomic diversity of picocyanobacteria. Time-calibration of a core-protein tree showed that gene gain/loss occurred at an unexpectedly low rate between the different lineages, with for instance 5.6 genes gained per million years (My) for the major Synechococcus lineage (sub-cluster 5.1), among which only 0.71/My have been fixed in the long term. Gene content comparisons revealed a number of candidates involved in nutrient adaptation, a large proportion of which are located in genomic islands shared between either closely or more distantly related strains, as identified using an original network construction approach. Interestingly, strains representative of the different ecotypes co-occurring in phosphorus-depleted waters (Synechococcus clades III, WPC1, and sub-cluster 5.3) were shown to display different adaptation strategies to this limitation. In contrast, we found few genes potentially involved in adaptation to temperature when comparing cold and warm thermotypes. Indeed, comparison of core protein sequences highlighted variants specific to cold thermotypes, notably involved in carotenoid biosynthesis and the oxidative stress response, revealing that long-term adaptation to thermal niches relies on amino acid substitutions rather than on gene content variation. Altogether, this study not only deciphers the respective roles of gene gains/losses and sequence variation but also uncovers numerous gene candidates lik
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- 2020
10. Traitement des leucémies aiguës
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Corre, E., primary, Marie, J. P., additional, Rio, B., additional, and Legrand, O., additional
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- 2011
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11. Oyster hemolymph is a complex and dynamic ecosystem hosting bacteria, protists and viruses.
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Dupont, S., primary, Lokmer, A., additional, Corre, E., additional, Auguet, J.-C., additional, Petton, B., additional, Toulza, E., additional, Montagnani, C., additional, Tanguy, G., additional, Pecqueur, D., additional, Salmeron, C., additional, Guillou, L., additional, Desnues, C., additional, Scola, B. La, additional, Khalil, J. Bou, additional, de Lorgeril, J., additional, Mitta, G., additional, Gueguen, Y., additional, and Escoubas, Jean-Michel, additional
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- 2019
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12. Long-term immune deficiency after allogeneic stem cell transplantation following a myeloablative conditioning regimen: O397
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Corre, E., Carmagnat, M., Busson, M., de Latour, Peffault R., Robin, M., Ribaud, P., Toubert, A., Rabian, C., and Socié, G.
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- 2009
13. Multiple patterns of mtDNA reorganization in plants regenerated from different in vitro cultured explants of a single wheat variety
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Morère-Le Paven, M. C., De Buyser, J., Henry, Y., Corre, E., Hartmann, C., and Rode, A.
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- 1992
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14. Proposition of Splenic Dose-Volume Constraints to Prevent Severe Lymphopenia after Chemoradiation for Pancreatic Cancer
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Blais, E., primary, Bourdais, R., additional, Simon, J.M., additional, Sargos, P., additional, Troussier, I., additional, Jenny, C., additional, Riet, F.G., additional, Canova, C.H., additional, Culot, F., additional, Le Corre, E., additional, Ozsahin, M., additional, and Maingon, P., additional
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- 2018
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15. A de novo approach to disentangle partner identity and function in holobiont systems
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Meng, A., Marchet, C., Corre, E., Peterlongo, P., Alberti, A., Da Silva, C., Wincker, P., Pelletier, E., Probert, I., Decelle, Johan, Le Crom, S., Not, F., Bittner, L., Meng, A., Marchet, C., Corre, E., Peterlongo, P., Alberti, A., Da Silva, C., Wincker, P., Pelletier, E., Probert, I., Decelle, Johan, Le Crom, S., Not, F., and Bittner, L.
- Abstract
BackgroundStudy of meta-transcriptomic datasets involving non-model organisms represents bioinformatic challenges. The production of chimeric sequences and our inability to distinguish the taxonomic origins of the sequences produced are inherent and recurrent difficulties in de novo assembly analyses. As the study of holobiont meta-transcriptomes is affected by challenges invoked above, we propose an innovative bioinformatic approach to tackle such difficulties and tested it on marine models as a proof of concept.ResultsWe considered three holobiont models, of which two transcriptomes were previously published and a yet unpublished transcriptome, to analyze and sort their raw reads using Short Read Connector, a k-mer based similarity method. Before assembly, we thus defined four distinct categories for each holobiont meta-transcriptome: host reads, symbiont reads, shared reads, and unassigned reads. Afterwards, we observed that independent de novo assemblies for each category led to a diminution of the number of chimeras compared to classical assembly methods. Moreover, the separation of each partner’s transcriptome offered the independent and comparative exploration of their functional diversity in the holobiont. Finally, our strategy allowed to propose new functional annotations for two well-studied holobionts (a Cnidaria-Dinophyta, a Porifera-Bacteria) and a first meta-transcriptome from a planktonic Radiolaria-Dinophyta system forming widespread symbiotic association for which our knowledge is considerably limited.ConclusionsIn contrast to classical assembly approaches, our bioinformatic strategy generates less de novo assembled chimera and allows biologists to study separately host and symbiont data from a holobiont mixture. The pre-assembly separation of reads using an efficient tool as Short Read Connector is an effective way to tackle meta-transcriptomic challenges and offers bright perpectives to study holobiont systems composed of either well-studied or po
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- 2018
16. Chimioradiothérapie des cancers du pancréas : pourquoi et comment protéger la rate ?
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Bourdais, R., primary, Troussier, I., additional, Epaillard, N., additional, Chauffert-Yvart, L., additional, Gérard, M., additional, Sargos, P., additional, Riet, F.-G., additional, Canova, C.-H., additional, Jenny, C., additional, Culot, F., additional, Le Corre, E., additional, Ozsahin, M., additional, Huguet, F., additional, Bachet, J.-B., additional, Simon, J.-M., additional, Maingon, P., additional, and Blais, E., additional
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- 2018
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17. Chloropicophyceae, a new class of picophytoplanktonic prasinophytes.
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Lopes dos-Santos, Antonina, Pollina, T., Gourvil, P., Corre, E., Marie, D., Garrido, José Luis, Rodríguez Hernández, Francisco José, Noël, M.H., Vaulot, Daniel, Eikrem, Wenche, Lopes dos-Santos, Antonina, Pollina, T., Gourvil, P., Corre, E., Marie, D., Garrido, José Luis, Rodríguez Hernández, Francisco José, Noël, M.H., Vaulot, Daniel, and Eikrem, Wenche
- Abstract
Prasinophytes are a paraphyletic group of nine lineages of green microalgae that are currently classified either at the class or order level or as clades without formal taxonomic description. Prasinophyte clade VII comprises picoplanktonic algae that are important components of marine phytoplankton communities, particularly in moderately oligotrophic waters. Despite first being cultured in the 1960s, this clade has yet to be formally described. Previous phylogenetic analyses using the 18S rRNA gene divided prasinophyte clade VII into three lineages, termed A, B and C, the latter formed by a single species, Picocystis salinarum, that to date has only been found in saline lakes. Strains from lineages A and B cannot be distinguished by light microscopy and have very similar photosynthetic pigment profiles corresponding to the prasino-2A pigment group. We obtained phenotypic and genetic data on a large set of prasinophyte clade VII culture strains that allowed us to clarify the taxonomy of this important marine group. We describe two novel classes, the Picocystophyceae and the Chloropicophyceae, the latter containing two novel genera, Chloropicon and Chloroparvula, and eight new species of marine picoplanktonic green algae
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- 2017
18. ROBOTS DURABLES D'ASSISTANCE ET DE SURVEILLANCE À FAIBLE COÛT
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Espes, David, Autret, Yvon, Vareille, J., Le Corre, E, Le Parc, Philippe, Lab-STICC_UBO_CID_IRIS, Institut Brestois du Numérique et des Mathématiques (IBNM), Université de Brest (UBO)-Université de Brest (UBO)-Laboratoire des sciences et techniques de l'information, de la communication et de la connaissance (Lab-STICC), École Nationale d'Ingénieurs de Brest (ENIB)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-École Nationale Supérieure de Techniques Avancées Bretagne (ENSTA Bretagne)-Institut Mines-Télécom [Paris] (IMT)-Centre National de la Recherche Scientifique (CNRS)-Université Bretagne Loire (UBL)-IMT Atlantique Bretagne-Pays de la Loire (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT)-École Nationale d'Ingénieurs de Brest (ENIB)-Université de Bretagne Sud (UBS)-École Nationale Supérieure de Techniques Avancées Bretagne (ENSTA Bretagne)-Institut Mines-Télécom [Paris] (IMT)-Centre National de la Recherche Scientifique (CNRS)-Université Bretagne Loire (UBL)-IMT Atlantique Bretagne-Pays de la Loire (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT), Institut Universitaire de Technologie - Brest/Morlaix (IUT Brest Morlaix), Université de Brest (UBO), and ADIUT
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méthodologie de conception ,[INFO.INFO-RB]Computer Science [cs]/Robotics [cs.RO] ,robot éco-conçu ,robot compagnon ,[SPI.MECA.GEME]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Mechanical engineering [physics.class-ph] ,[SPI.AUTO]Engineering Sciences [physics]/Automatic - Abstract
Le fichier de 2014 était la communication acceptée, mais le congrès de 2014 n'a pas eu lieu l'ADIUT l'ayant annulé au cours du mois de mai.En 2015 le format proposé aux auteurs était beaucoup plus concis qu'en 2014. Nous avons décidé de proposer un article sur le même sujet qu'en 2014 en réduisant le document à ce qui était demandé en 2015.Le congrès de 2015 a bien eu lieu.; National audience; En Europe le nombre de personnes âgées et de personnes handicapées dépendantes va augmenter dans les prochaines années. Pour éviter une dégradation de leur qualité de vie, celles-ci peuvent être assistées à domicile. Cette contribution a pour sujet les robots compagnons durables pour les personnes dépendantes. Nos robots sont contrôlés à distance afin d’assurer un monitoring et maintenir des relations sociales. Soignants ou parents peuvent contrôler chaque robot afin d'interagir avec la personne dépendante. Au cours de la phase de conception, nous visons un coût de robot aussi bas que possible, car c’est un facteur clé dans la généralisation de ceux-ci. Dans la perspective d’une production en grand nombre, afin de respecter les contraintes environnementales et de réduire la quantité de déchets, les robots doivent être conçus de façon appropriée. Nous proposons une méthodologie d’ingénierie, la méthode de développement en X, pour guider la conception de nouveaux produits de bout en bout. Notre but est de proposer une démarche de développement selon un ordonnancement des tâches qui permette d’orienter les choix vers la réduction des, impacts environnementaux en vue d’un marché de masse.
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- 2015
19. Responses of the arcto-boreal krill species Thysanoessa inermis to variations in water temperature: coupling Hsp70 isoform expressions with metabolism
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Huenerlage, Kim, Cascella, Kévin, Corre, E., Toomey, L., Lee, C.Y., Buchholz, Friedrich, Toullec, Jean-Yves, Huenerlage, Kim, Cascella, Kévin, Corre, E., Toomey, L., Lee, C.Y., Buchholz, Friedrich, and Toullec, Jean-Yves
- Abstract
Recent studies have indicated a metabolic temperature sensitivity in both the arcto-boreal krill species Thysanoessa inermis and Thysanoessa raschii that may determine these species' abundance and population persistence at lower latitudes (up to 40° N). T. inermis currently dominates the krill community in the Barents Sea and in the high Arctic Kongsfjord. We aimed to increase the knowledge on the upper thermal limit found in the latter species by estimating the CT50 value (19.7 °C) (critical temperature at which 50 % of animals are reactive) and by linking metabolic rate measurements with molecular approaches. Optical oxygen sensors were used to measure respiration rates in steps of 2 °C (from 0 to 16 °C). To follow the temperature-mediated mechanisms of passive response, i.e., as a proxy for molecular stress, molecular chaperone heat shock protein 70 (Hsp70) sequences were extracted from a transcriptome assembly, and the gene expression kinetics were monitored during an acute temperature exposure to 6 or 10 °C with subsequent recovery at 4 °C. Our results showed upregulation of hsp70 genes, especially the structurally constitutive and mitochondrial isoforms. These findings confirmed the temperature sensitivity of T. inermis and showed that the thermal stress took place before reaching the upper temperature limit estimated by respirometry at 12 °C. This study provides a baseline for further investigations into the thermal tolerances of arcto-boreal Thysanoessa spp. and comparisons with other krill species under different climatic regimes, especially Antarctica.
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- 2016
20. L’activité de télémédecine dans les microangiopathies thrombotiques : un progrès dans les maladies rares nécessitant une prise en charge en urgence
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Coppo, P., primary, Corre, E., additional, Rondeau, E., additional, Benhamou, Y., additional, Bachet, A., additional, Stépanian, A., additional, and Veyradier, A., additional
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- 2016
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21. The Ectocarpus genome and brown algal genomics
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Cock, J.M., Sterck, L., Ahmed, S., Allen, A. E., Amoutzias, G., Anthouard, V., Artiguenave, F., Arun, A., Aury, J. M., Badger, J. H., Beszteri, B., Billiau, K., Bonnet, E., Bothwell, J. H., Bowler, C., Boyen, C., Brownlee, C., Carrano, C. J., Charrier, B., Cho, G. Y., Coelho, S. M., Collen, J., Le Corguille, G., Corre, E., Dartevelle, L., Da Silva, C., Delage, L., Delaroque, N., Dittami, S. M., Doulbeau, Sylvie, Elias, M., Farnham, G., Gachon, C. M. M., Godfroy, O., Gschloessl, B., Heesch, S., Jabbari, K., Jubin, C., Kawai, H., Kimura, K., Kloareg, B., Kuepper, F. C., Lang, D., Le Bail, A., Luthringer, R., Leblanc, C., Lerouge, P., Lohr, M., Lopez, P. J., Macaisne, N., Martens, C., Maumus, F., Michel, G., Miranda-Saavedra, D., Morales, J., Moreau, H., Motomura, T., Nagasato, C., Napoli, C. A., Nelson, D. R., Nyvall-Collen, P., Peters, A. F., Pommier, C., Potin, P., Poulain, J., Quesneville, H., Read, B., Rensing, S.A., Ritter, A., Rousvoal, S., Samanta, M., Samson, G., Schroeder, D.C., Scornet, D., Segurens, B., Strittmatter, M., Tonon, T., Tregear, James, Valentin, K., Von Dassow, P., Yamagishi, T., Rouze, P., Van de Peer, Y., Wincker, P., and Piganeau, G. (ed.)
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IRON-DEFICIENCY ,EVOLUTIONARY HISTORY ,PLANT MICRORNAS ,SILICULOSUS ,TOPOISOMERASE-VI ,fungi ,food and beverages ,DIATOM THALASSIOSIRA-PSEUDONANA ,PROTEIN FAMILY ,LAMINARIA-DIGITATA ,CHLAMYDOMONAS-REINHARDTII ,PHAEOPHYCEAE ,NORTH-ATLANTIC - Abstract
Brown algae are important organisms both because of their key ecological roles in coastal ecosystems and because of the remarkable biological features that they have acquired during their unusual evolutionary history. The recent sequencing of the complete genome of the filamentous brown alga Ectocarpus has provided unprecedented access to the molecular processes that underlie brown algal biology. Analysis of the genome sequence, which exhibits several unusual structural features, identified genes that are predicted to play key roles in several aspects of brown algal metabolism, in the construction of the multicellular bodyplan and in resistance to biotic and abiotic stresses. Information from the genome sequence is currently being used in combination with other genomic, genetic and biochemical tools to further investigate these and other aspects of brown algal biology at the molecular level. Here, we review some of the major discoveries that emerged from the analysis of the Ectocarpus genome sequence, with a particular focus on the unusual genome structure, inferences about brown algal evolution and novel aspects of brown algal metabolism.
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- 2012
22. Efficacité et tolérance de la tomothérapie hélicoïdale des méningiomes intracrâniens de grade II-III : Expérience de l’hôpital de la Pitié-Salpêtrière
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Boulle, G., primary, Bracci, S., additional, Le Corre, E., additional, Clausse, E., additional, Belghith, B., additional, Kamsu Kom, L., additional, Chevalier, A., additional, Mazeron, J. -J., additional, and Feuvret, L., additional
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- 2015
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23. The Ectocarpus genome and the independent evolution of multicellularity in brown algae
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Cock, J.M., Sterck, L., Rouzé, P., Scornet, D., Allen, A.E., Amoutzias, G., Anthouard, V., Artiguenave, F., Aury, J.-M., Badger, J.H., Beszteri, B., Billiau, K., Bonnet, E., Bothwell, J.H., Bowler, C., Boyen, C., Brownlee, C., Carrano, C.J., Charrier, B., Cho, G.Y., Coelho, S.M., Collén, J., Corre, E., Da Silva, C., Delage, L., Delaroque, N., Dittami, S.M., Doulbeau, S., Elias, M., Farnham, G., Gachon, C.M.M., Gschloessl, B., Heesch, S., Jabbari, K., Jubin, C., Kawai, H., Kimura, K., Kloareg, B., Küpper, F.C., Lang, D., Le Bail, A., Leblanc, C., Lerouge, P., Lohr, M., Lopez, P.J., Martens, C., Maumus, F., Michel, G., Miranda-Saavedra, D., Morales, J., Moreau, H., Motomura, T., Nagasato, C., Napoli, C.A., Nelson, D.R., Nyvall-Collén, P., Peters, A.F., Pommier, C., Potin, Ph., Poulain, J., Quesneville, H., Read, B., Rensing, S.A., Ritter, A., Rousvoal, S., Samanta, M., Samson, G., Schroeder, D.C., Segurens, B., Strittmatter, M., Tonon, T., Tregear, J.W., Valentin, K., von Dassow, P., Yamagishi, T., Van de Peer, Y., and Wincker, P.
- Abstract
Brown algae (Phaeophyceae) are complex photosynthetic organisms with a very different evolutionary history to green plants, to which they are only distantly related1. These seaweeds are the dominant species in rocky coastal ecosystems and they exhibit many interesting adaptations to these, often harsh, environments. Brown algae are also one of only a small number of eukaryotic lineages that have evolved complex multicellularity (Fig. 1).We report the 214 million base pair (Mbp) genome sequence of the filamentous seaweed Ectocarpus siliculosus (Dillwyn) Lyngbye, a model organism for brown algae, closely related to the kelps (Fig. 1). Genome features such as the presence of an extended set of light-harvesting and pigment biosynthesis genes and new metabolic processes such as halide metabolism help explain the ability of this organism to cope with the highly variable tidal environment. The evolution of multicellularity in this lineage is correlated with the presence of a rich array of signal transduction genes. Of particular interest is the presence of a family of receptor kinases, as the independent evolution of related molecules has been linked with the emergence of multicellularity in both the animal and green plant lineages. The Ectocarpus genome sequence represents an important step towards developing this organism as a model species, providing the possibility to combine genomic and genetic2 approaches to explore these and other aspects of brown algal biology further.
- Published
- 2010
24. Comparative Transcriptome of Wild Type and Selected Strains of the Microalgae Tisochrysis lutea Provides Insights into the Genetic Basis, Lipid Metabolism and the Life Cycle
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Carrier, Gregory, Garnier, Matthieu, Le Cunff, Loic, Bougaran, Gael, Probert, Ian, De Vargas, Colomban, Corre, E, Cadoret, Jean-paul, Saint-jean, Bruno, Carrier, Gregory, Garnier, Matthieu, Le Cunff, Loic, Bougaran, Gael, Probert, Ian, De Vargas, Colomban, Corre, E, Cadoret, Jean-paul, and Saint-jean, Bruno
- Abstract
The applied exploitation of microalgae cultures has to date almost exclusively involved the use of wild type strains, deposited over decades in dedicated culture collections. Concomitantly, the concept of improving algae with selection programs for particular specific purposes is slowly emerging. Studying since a decade an economically and ecologically important haptophyte Tisochrysis lutea (Tiso), we took advantage of the availability of wild type (Tiso-Wt) and selected (Tiso-S2M2) strains to conduct a molecular variations study. This endeavour presented substantial challenges: the genome assembly was not yet available, the life cycle unknown and genetic diversity of Tiso-Wt poorly documented. This study brings the first molecular data in order to set up a selection strategy for that microalgae. Following high-throughput Illumina sequencing, transcriptomes of Tiso-Wt and Tiso-S2M2 were de novo assembled and annotated. Genetic diversity between both strains was analyzed and revealed a clear conservation, while a comparison of transcriptomes allowed identification of polymorphisms resulting from the selection program. Of 34,374 transcripts, 291 were differentially expressed and 165 contained positional polymorphisms (SNP, Indel). We focused on lipid over-accumulation of the Tiso-S2M2 strain and 8 candidate genes were identified by combining analysis of positional polymorphism, differential expression levels, selection signature and by study of putative gene function. Moreover, genetic analysis also suggests the existence of a sexual cycle and genetic recombination in Tisochrysis lutea.
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- 2014
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25. Identification of stress gene transcripts in Laminaria digitata (Phaeophyceae) protoplast cultures by expressed sequence tag analysis
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V. Collen J. Rousvoal S. Corre E. Leblanc C. And Boyen C., Roeder, Signaux oligosaccharidiques et lipidiques (SOL), Station biologique de Roscoff [Roscoff] (SBR), and Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-GOEMAR-Centre National de la Recherche Scientifique (CNRS)
- Published
- 2005
26. The Ectocarpus genome and the independent evolution of multicellularity in the brown algae
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Cock, J. M., Sterck, L., Rouze, P., Scorne, D., Allen, A. E., Amoutzias, G., Anthouard, V., Artiguenave, F., Aury, J. M., Badger, J. H., Beszteri, Bank, Billiau, K., Bonnet, E., Bothwell, J. H. F., Bowler, C., Boyen, C., Brownlee, C., Carrano, C. J., Charrier, B., Youn Cho, G., Coelho, S. M., Collén, J., Corre, E., Da Silva, C., Delage, L., Delaroque, N., Dittami, S. M., Doulbeau, S., Elias, M., Farnham, C., Gachon, C. M. M., Gschloessl, B., Heesch, S., Jabbari, K., Jubin, C., Kawai, H., Kimura, K., Kloareg, B., Küpper, F. C., Lang, D., Le Bail, A., Leblan, C., Lerouge, P., Lohr, M., López-González, P. J., Martens, C., Maumus, F., Michel, G., Miranda-Saavedra, D., Morales, J., Moreau, H., Motomura, T., Nagasato, C., Napoli, C. A., Nelson, D. R., Nyvall-Collén, Pi, Peters, A. F., Pommier, C., Potin, P., Poulain, J., Quesneville, H., Read, B., Rensing, S. A., Ritter, A., Rousvoal, S., Samanta, M., Samson, G., Schroeder, D. C., Séguren, B., Strittmatter, M., Tonon, T., Tregear, J., Valentin, Klaus-Ulrich, von Dassow, P., Yamagishi, T., Van de Peer, Y., Wincker, P., Cock, J. M., Sterck, L., Rouze, P., Scorne, D., Allen, A. E., Amoutzias, G., Anthouard, V., Artiguenave, F., Aury, J. M., Badger, J. H., Beszteri, Bank, Billiau, K., Bonnet, E., Bothwell, J. H. F., Bowler, C., Boyen, C., Brownlee, C., Carrano, C. J., Charrier, B., Youn Cho, G., Coelho, S. M., Collén, J., Corre, E., Da Silva, C., Delage, L., Delaroque, N., Dittami, S. M., Doulbeau, S., Elias, M., Farnham, C., Gachon, C. M. M., Gschloessl, B., Heesch, S., Jabbari, K., Jubin, C., Kawai, H., Kimura, K., Kloareg, B., Küpper, F. C., Lang, D., Le Bail, A., Leblan, C., Lerouge, P., Lohr, M., López-González, P. J., Martens, C., Maumus, F., Michel, G., Miranda-Saavedra, D., Morales, J., Moreau, H., Motomura, T., Nagasato, C., Napoli, C. A., Nelson, D. R., Nyvall-Collén, Pi, Peters, A. F., Pommier, C., Potin, P., Poulain, J., Quesneville, H., Read, B., Rensing, S. A., Ritter, A., Rousvoal, S., Samanta, M., Samson, G., Schroeder, D. C., Séguren, B., Strittmatter, M., Tonon, T., Tregear, J., Valentin, Klaus-Ulrich, von Dassow, P., Yamagishi, T., Van de Peer, Y., and Wincker, P.
- Published
- 2010
27. Élévation chronique de l’urée sans insuffisance rénale, ni acidose métabolique après entérocystoplastie
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Estève, E., primary, Lepeytre, F., additional, Haymann, J.P., additional, Corre, E., additional, Ronco, P., additional, and Boffa, J.J., additional
- Published
- 2013
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28. Vibrations, un risque mal connu des entreprises et des conducteurs d’engins de manutention et de chantier
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Vilaine, C., primary, Petitfour, R., additional, Dupéry, M., additional, Fabin, C., additional, Le Corre, E., additional, Montchamp, E., additional, Montéléon, P.Y., additional, Nicolazzo, P., additional, and Wargon, C., additional
- Published
- 2012
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29. Hernie intraspongieuse hypermétabolique en TEP/TDM au 18-FDG : à propos d’un cas
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Robin, P., primary, Le Roux, P.Y., additional, Bouttier, R., additional, Corre, E. Billet-Le, additional, Abgral, R., additional, Querellou, S., additional, Cavarec, M., additional, and Salaun, P.Y., additional
- Published
- 2012
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30. ATP Binding Cassette transporters associated with chemoresistance: transcriptional profiling in extreme cohorts and their prognostic impact in a cohort of 281 acute myeloid leukemia patients
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Marzac, C., primary, Garrido, E., additional, Tang, R., additional, Fava, F., additional, Hirsch, P., additional, De Benedictis, C., additional, Corre, E., additional, Lapusan, S., additional, Lallemand, J.-Y., additional, Marie, J.-P., additional, Jacquet, E., additional, and Legrand, O., additional
- Published
- 2011
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31. Long-term immune deficiency after allogeneic stem cell transplantation: B-cell deficiency is associated with late infections
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Corre, E., primary, Carmagnat, M., additional, Busson, M., additional, de Latour, R. P., additional, Robin, M., additional, Ribaud, P., additional, Toubert, A., additional, Rabian, C., additional, and Socie, G., additional
- Published
- 2010
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32. Étude de la pénibilité de la manutention manuelle des conteneurs d’ordures sur les sites d’un gestionnaire de logements en Île-de-France
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Levert, M., primary, Bruneteau, P., additional, Le Corre, E., additional, Alcouffe, J., additional, and Montéléon, P.-Y., additional
- Published
- 2008
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33. Measurement of Carbon Dioxide Solubility in a Solution of Diethanolamine Mixed with Methanol
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Tounsi, K. N. Habchi, primary, Barreau, A., additional, Le Corre, E., additional, Mougin, P., additional, and Neau, E., additional
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- 2005
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34. Petroleum Reservoirs, Microbial Diversity in
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Kendall, M. M., primary, Reysenbach, Anna-Louise, additional, Jeanthon, C., additional, and Corre, E., additional
- Published
- 2003
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35. ε-Proteobacterial diversity from a deep-sea hydrothermal vent on the Mid-Atlantic Ridge
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Corre, E, primary
- Published
- 2001
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36. Zobellia galactanovorans gen. nov., sp. nov., a marine species of Flavobacteriaceae isolated from a red alga, and classification of [Cytophaga] uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Zobellia uliginosa gen. nov., comb. nov.
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Barbeyron, T, primary, L'Haridon, S, additional, Corre, E, additional, Kloareg, B, additional, and Potin, P, additional
- Published
- 2001
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37. Methanococcus vulcanius sp. nov., a novel hyperthermophilic methanogen isolated from East Pacific Rise, and identification of Methanococcus sp. DSM 4213Tas Methanococcus fervens sp. nov.
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Jeanthon, C., primary, L'Haridon, S., additional, Reysenbach, A.-L., additional, Corre, E., additional, Vernet, M., additional, Messner, P., additional, Sleytr, U. B., additional, and Prieur, D., additional
- Published
- 1999
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38. Multiple patterns of mtDNA reorganization in plants regenerated from different in vitro cultured explants of a single wheat variety.
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Morère-Le Paven, M., Buyser, J., Henry, Y., Corre, E., Hartmann, C., and Rode, A.
- Abstract
We have previously shown that tissue cultures derived from various explants of the wheat variety Chinese Spring exhibit organ/tissue-specific changes in the organization of their mitochondrial genome. The aim of this work was to study the influence of passage out of in-vitro culture, and subsequent plant regeneration, on the in vitro 'induced' reorganization of this genome. In all cases but one, subgenomic configurations present in both the donor parent and the tissue culture were evident, in corresponding regenerated plants. The presence, in regenerated plants, of subgenomic configurations found in tissue culture but undetectable in the donor parent appeared to be both timeand organ/tissue-dependent. Moreover, when present, these novel organizations were not systematically found in all regenerated plants. Finally, novel subgenomic configurations were specifically detected after passage out of in-vitro culture. As these results were obtained from a single plant variety, they clearly confirm the extreme plasticity of mitochondrial genome structure in response to in-vitro culture. [ABSTRACT FROM AUTHOR]
- Published
- 1993
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39. Conjugating effects of symbionts and environmental factors on gene expression in deep-sea hydrothermal vent mussels
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Corre Erwan, Dossat Carole, Lecompte Odile, Ripp Raymond, Boutet Isabelle, Tanguy Arnaud, and Lallier François H
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Bathymodiolus azoricus ,symbiosis ,gene expression ,environment ,Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background The deep-sea hydrothermal vent mussel Bathymodiolus azoricus harbors thiotrophic and methanotrophic symbiotic bacteria in its gills. While the symbiotic relationship between this hydrothermal mussel and these chemoautotrophic bacteria has been described, the molecular processes involved in the cross-talking between symbionts and host, in the maintenance of the symbiois, in the influence of environmental parameters on gene expression, and in transcriptome variation across individuals remain poorly understood. In an attempt to understand how, and to what extent, this double symbiosis affects host gene expression, we used a transcriptomic approach to identify genes potentially regulated by symbiont characteristics, environmental conditions or both. This study was done on mussels from two contrasting populations. Results Subtractive libraries allowed the identification of about 1000 genes putatively regulated by symbiosis and/or environmental factors. Microarray analysis showed that 120 genes (3.5% of all genes) were differentially expressed between the Menez Gwen (MG) and Rainbow (Rb) vent fields. The total number of regulated genes in mussels harboring a high versus a low symbiont content did not differ significantly. With regard to the impact of symbiont content, only 1% of all genes were regulated by thiotrophic (SOX) and methanotrophic (MOX) bacteria content in MG mussels whereas 5.6% were regulated in mussels collected at Rb. MOX symbionts also impacted a higher proportion of genes than SOX in both vent fields. When host transcriptome expression was analyzed with respect to symbiont gene expression, it was related to symbiont quantity in each field. Conclusions Our study has produced a preliminary description of a transcriptomic response in a hydrothermal vent mussel host of both thiotrophic and methanotrophic symbiotic bacteria. This model can help to identify genes involved in the maintenance of symbiosis or regulated by environmental parameters. Our results provide evidence of symbiont effect on transcriptome regulation, with differences related to type of symbiont, even though the relative percentage of genes involved remains limited. Differences observed between the vent site indicate that environment strongly influences transcriptome regulation and impacts both activity and relative abundance of each symbiont. Among all these genes, those participating in recognition, the immune system, oxidative stress, and energy metabolism constitute new promising targets for extended studies on symbiosis and the effect of environmental parameters on the symbiotic relationships in B. azoricus.
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- 2011
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40. Plastid genomes of two brown algae, Ectocarpus siliculosus and Fucus vesiculosus: further insights on the evolution of red-algal derived plastids
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Corre Erwan, Gschloessl Bernhard, Viegas Carla, Valente Marta, Pearson Gareth, Le Corguillé Gildas, Bailly Xavier, Peters Akira F, Jubin Claire, Vacherie Benoit, Cock J Mark, and Leblanc Catherine
- Subjects
Evolution ,QH359-425 - Abstract
Abstract Background Heterokont algae, together with cryptophytes, haptophytes and some alveolates, possess red-algal derived plastids. The chromalveolate hypothesis proposes that the red-algal derived plastids of all four groups have a monophyletic origin resulting from a single secondary endosymbiotic event. However, due to incongruence between nuclear and plastid phylogenies, this controversial hypothesis remains under debate. Large-scale genomic analyses have shown to be a powerful tool for phylogenetic reconstruction but insufficient sequence data have been available for red-algal derived plastid genomes. Results The chloroplast genomes of two brown algae, Ectocarpus siliculosus and Fucus vesiculosus, have been fully sequenced. These species represent two distinct orders of the Phaeophyceae, which is a major group within the heterokont lineage. The sizes of the circular plastid genomes are 139,954 and 124,986 base pairs, respectively, the size difference being due principally to the presence of longer inverted repeat and intergenic regions in E. siliculosus. Gene contents of the two plastids are similar with 139-148 protein-coding genes, 28-31 tRNA genes, and 3 ribosomal RNA genes. The two genomes also exhibit very similar rearrangements compared to other sequenced plastid genomes. The tRNA-Leu gene of E. siliculosus lacks an intron, in contrast to the F. vesiculosus and other heterokont plastid homologues, suggesting its recent loss in the Ectocarpales. Most of the brown algal plastid genes are shared with other red-algal derived plastid genomes, but a few are absent from raphidophyte or diatom plastid genomes. One of these regions is most similar to an apicomplexan nuclear sequence. The phylogenetic relationship between heterokonts, cryptophytes and haptophytes (collectively referred to as chromists) plastids was investigated using several datasets of concatenated proteins from two cyanobacterial genomes and 18 plastid genomes, including most of the available red algal and chromist plastid genomes. Conclusion The phylogenetic studies using concatenated plastid proteins still do not resolve the question of the monophyly of all chromist plastids. However, these results support both the monophyly of heterokont plastids and that of cryptophyte and haptophyte plastids, in agreement with nuclear phylogenies.
- Published
- 2009
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41. P-gp activity is a critical resistance factor against AVE9633 and DM4 cytotoxicity in leukaemia cell lines, but not a major mechanism of chemoresistance in cells from acute myeloid leukaemia patients
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Fava Fanny, Morjani Hamid, Marjanovic Zora, Zuany-Amorim Claudia, Faussat Anne-Marie, Perrot Jean-Yves, Cohen Simy, Tang Ruoping, Corre Elise, Legrand Ollivier, and Marie Jean-Pierre
- Subjects
Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background AVE9633 is a new immunoconjugate comprising a humanized monoclonal antibody, anti-CD33 antigen, linked through a disulfide bond to the maytansine derivative DM4, a cytotoxic agent and potent tubulin inhibitor. It is undergoing a phase I clinical trial. Chemoresistance to anti-mitotic agents has been shown to be related, in part, to overexpression of ABC proteins. The aim of the present study was to investigate the potential roles of P-gp, MRP1 and BCRP in cytotoxicity in AVE9633-induced acute myeloid leukaemia (AML). Methods This study used AML cell lines expressing different levels of P-gp, MRP1 or BCRP proteins and twenty-five samples from AML patients. Expression and functionality of the transporter protein were analyzed by flow cytometry. The cytotoxicity of the drug was evaluated by MTT and apoptosis assays. Results P-gp activity, but not MRP1 and BCRP, attenuated AVE9633 and DM4 cytotoxicity in myeloid cell lines. Zosuquidar, a potent specific P-gp inhibitor, restored the sensitivity of cells expressing P-gp to both AVE9633 and DM4. However, the data from AML patients show that 10/25 samples of AML cells (40%) were resistant to AVE9633 or DM4 (IC50 > 500 nM), and this was not related to P-gp activity (p-Value: 0.7). Zosuquidar also failed to re-establish drug sensitivity. Furthermore, this resistance was not correlated with CD33 expression (p-Value: 0.6) in those cells. Conclusion P-gp activity is not a crucial mechanism of chemoresistance to AVE9633. For patients whose resistance to conventional anthracycline AML regimens is related to ABC protein expression, a combination with AVE9633 could be beneficial. Other mechanisms such as microtubule alteration could play an important role in chemoresistance to AVE9633.
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- 2009
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42. Les constructions à verbes multiples en khmer
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Paillard, Denis, Laboratoire de Linguistique Formelle (LLF UMR7110), Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7), Corre, E, and Paillard, Denis
- Subjects
C-ACTI - Published
- 2013
43. Candidate genes involved in biosynthesis and degradation of the main extracellular matrix polysaccharides of brown algae and their probable evolutionary history.
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Mazéas L, Bouguerba-Collin A, Cock JM, Denoeud F, Godfroy O, Brillet-Guéguen L, Barbeyron T, Lipinska AP, Delage L, Corre E, Drula E, Henrissat B, Czjzek M, Terrapon N, and Hervé C
- Subjects
- Alginates metabolism, Genomics methods, Phaeophyceae genetics, Phaeophyceae metabolism, Phylogeny, Polysaccharides biosynthesis, Polysaccharides metabolism, Extracellular Matrix metabolism, Evolution, Molecular
- Abstract
Background: Brown algae belong to the Stramenopiles phylum and are phylogenetically distant from plants and other multicellular organisms. This independent evolutionary history has shaped brown algae with numerous metabolic characteristics specific to this group, including the synthesis of peculiar polysaccharides contained in their extracellular matrix (ECM). Alginates and fucose-containing sulphated polysaccharides (FCSPs), the latter including fucans, are the main components of ECMs. However, the metabolic pathways of these polysaccharides remain poorly described due to a lack of genomic data., Results: An extensive genomic dataset has been recently released for brown algae and their close sister species, for which we previously performed an expert annotation of key genes involved in ECM-carbohydrate metabolisms. Here we provide a deeper analysis of this set of genes using comparative genomics, phylogenetics analyses, and protein modelling. Two key gene families involved in both the synthesis and degradation of alginate were suggested to have been acquired by the common ancestor of brown algae and their closest sister species Schizocladia ischiensis. Our analysis indicates that this assumption can be extended to additional metabolic steps, and thus to the whole alginate metabolic pathway. The pathway for the biosynthesis of fucans still remains biochemically unresolved and we also investigate putative fucosyltransferase genes that may harbour a fucan synthase activity in brown algae., Conclusions: Our analysis is the first extensive survey of carbohydrate-related enzymes in brown algae, and provides a valuable resource for future research into the glycome and ECM of brown algae. The expansion of specific families related to alginate metabolism may have represented an important prerequisite for the evolution of developmental complexity in brown algae. Our analysis questions the possible occurrence of FCSPs outside brown algae, notably within their closest sister taxon and in other Stramenopiles such as diatoms. Filling this knowledge gap in the future will help determine the origin and evolutionary history of fucan synthesis in eukaryotes., (© 2024. The Author(s).)
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- 2024
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44. Efficacy and safety of daratumumab in multiresistant immune-mediated thrombotic thrombocytopenic purpura.
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Weisinger J, Bouzid R, Ranta D, Woaye-Hune P, Cohen-Aubart F, Gaible C, Marjanovic Z, Corre E, Joly AC, Baylatry MT, Joly BS, Veyradier A, and Coppo P
- Abstract
The immunosuppressive treatment of immune-mediated thrombotic thrombocytopenic purpura (iTTP) in patients with intolerance or refractoriness to the B-cell depleting monoclonal antibody rituximab remains debated. Daratumumab, a plasma cell-directed monoclonal antibody targeting CD38, represents a therapeutic option, but data are scarce. The French Thrombotic Microangiopathies Reference Center conducted a nationwide survey on iTTP patients treated with daratumumab. Nine episodes from seven patients were identified. Treatment was administered for A Disintegrin And Metalloproteinase with ThromboSpondin-1 motifs, 13th member (ADAMTS13) relapses while patients were otherwise in clinical response (N = 8), or during the acute phase of the disease following rituximab intolerance (N = 1). Patients have received a median of three previous therapeutic lines. ADAMTS13 activity improved in eight cases following daratumumab administration, including three cases where ADAMTS13 normalized. ADAMTS13 relapses occurred in three patients; in two cases, retreatment with daratumumab was successful. Median ADAMTS13 relapse-free survival was not reached; 12-month ADAMTS13 relapse-free survival was 56%. Daratumumab-related adverse events occurred in five cases and were non-severe infusion-related reactions in all cases. These results suggest that daratumumab may be an effective treatment option for iTTP patients with intolerance or refractoriness to rituximab., (© 2024 The Author(s). British Journal of Haematology published by British Society for Haematology and John Wiley & Sons Ltd.)
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- 2024
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45. Transcriptomic responses of Antarctic clam Laternula elliptica to nanoparticles, at single and combined exposures reveal ecologically relevant biomarkers.
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Rondon R, Valdés C, Cosseau C, Bergami E, Cárdenas CA, Balbi T, Pérez-Toledo C, Garrido I, Perrois G, Chaparro C, Corre E, Corsi I, and González-Aravena M
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- Animals, Antarctic Regions, Biomarkers metabolism, Polystyrenes toxicity, Environmental Monitoring methods, Bivalvia drug effects, Bivalvia genetics, Titanium toxicity, Nanoparticles toxicity, Transcriptome drug effects, Water Pollutants, Chemical toxicity
- Abstract
In recent years micro- and nanoplastics and metal-oxide nanomaterials have been found in several environmental compartments. The Antarctic soft clam Laternula elliptica is an endemic Antarctic species having a wide distribution in the Southern Ocean. Being a filter-feeder, it could act as suitable bioindicator of pollution from nanoparticles also considering its sensitivity to various sources of stress. The present study aims to assess the impact of polystyrene nanoparticles (PS-NP) and the nanometal titanium-dioxide (n-TiO
2 ) on genome-wide transcript expression of L. elliptica either alone and in combination and at two toxicological relevant concentrations (5 and 50 µg/L) during 96 h exposure. Transcript-target qRT-PCR was performed with the aim to identify suitable biomarkers of exposure and effects. As expected, at the highest concentration tested, the clustering was clearer between control and exposed clams. A total of 221 genes resulted differentially expressed in exposed clams and control ones, and 21 of them had functional annotation such as ribosomal proteins, antioxidant, ion transport (osmoregulation), acid-base balance, immunity, lipid metabolism, cell adhesion, cytoskeleton, apoptosis, chromatin condensation and cell signaling. At functional level, relevant transcripts were shared among some treatments and could be considered as general stress due to nanoparticle exposure. After applying transcript-target approach duplicating the number of clam samples, four ecologically relevant transcripts were revealed as biomarkers for PS-NP, n-TiO2 and their combination at 50 µg/L, that could be used for monitoring clams' health status in different Antarctic localities., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)- Published
- 2024
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46. A combination of 5-azacytidine and nivolumab is a potentially effective rescue therapy in relapsed/refractory AITL.
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Ricard L, Cervera P, Stocker N, Corre E, Van de Wyngaert Z, Banet A, Marjanovic Z, Dulery R, Bravetti C, Joly AC, Baylatry MT, and Coppo P
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- Humans, Female, Male, Aged, Middle Aged, Neoplasm Recurrence, Local drug therapy, Lymphoma, T-Cell, Peripheral drug therapy, Lymphoma, T-Cell, Peripheral mortality, Treatment Outcome, Aged, 80 and over, Drug Resistance, Neoplasm, Immune Checkpoint Inhibitors therapeutic use, Immune Checkpoint Inhibitors adverse effects, Nivolumab therapeutic use, Azacitidine therapeutic use, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Antineoplastic Combined Chemotherapy Protocols adverse effects
- Abstract
Introduction: Angioimmunoblastic T-cell lymphoma (AITL) is a peripheral T-cell lymphoma characterized by a T follicular helper cell phenotype expressing PD-1 (programmed cell death-1). AITL exhibits a poor response to conventional chemotherapy, with a median 5-year overall survival of 44% and a progression-free survival of 32%. Relapse is common, resulting in a median overall survival of 6 months. Recurrent mutations are detected in genes regulating DNA methylation, including TET2, DNMT3A, and IDH2 variants, along with the prevalent RHOA G17V mutation. In this context, patients treated with the hypomethylating agent 5-azacytidine achieved overall response and complete response rates of 75% and 41%, respectively. We hypothesized that targeted therapies combining anti-PD-1 checkpoint blockers with hypomethylating agents could be efficient in AITL patients and less toxic than standard chemotherapy., Methods: Here, we report the efficacy of a regimen combining 5-azacytidine and nivolumab in nine relapsed or refractory AITL patients., Results: This regimen was well-tolerated, especially in elderly patients. The overall response rate was 78%, including four partial responses (44%) and three complete responses (33%). Allogeneic hematopoietic stem cell transplantation was performed in two patients who reached complete response., Discussion: These preliminary favorable results may serve as a basis for further investigation in prospective studies., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Ricard, Cervera, Stocker, Corre, Van de Wyngaert, Banet, Marjanovic, Dulery, Bravetti, Joly, Baylatry and Coppo.)
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- 2024
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47. Differential prognostic values of the three AKT isoforms in acute myeloid leukemia.
- Author
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Corre E, Soum C, Pfeifer R, Bessière C, Dailhau S, Marbœuf C, Meggetto F, Touriol C, Récher C, Bousquet M, and Pyronnet S
- Subjects
- Humans, Core Binding Factor Alpha 2 Subunit genetics, Mutation, Phosphatidylinositol 3-Kinases genetics, Prognosis, Protein Isoforms genetics, Protein Isoforms metabolism, Leukemia, Myeloid, Acute genetics, Proto-Oncogene Proteins c-akt genetics, Proto-Oncogene Proteins c-akt metabolism
- Abstract
The PI3K-AKT-mTOR pathway lies at the confluence of signaling pathways in which various components are subjected to activating genetic alterations in acute myeloid leukemia (AML), thus contributing to oncogenesis. Three AKT isoforms exist in humans. However, whether one isoform predominates in AML remains unknown. This study reveals that AKT3 behaves very distinctly than AKT1 or AKT2 in both normal myeloid differentiation and AML. During normal differentiation, AKT3 is preferentially expressed in hematopoietic stem cells whilst AKT1 becomes preferentially expressed as cells differentiate into granulocytes or monocytes. AKT2 expression remains unchanged. In AML, AKT3 expression varies widely among patient samples and is counterintuitively high in mature/monocytic leukemia. Furthermore, a low level of AKT3 expression is strongly correlated to genetic alterations associated with a better outcome (NPM1 mutations and RUNX1-RUNX1T1 translocation), while a high level is correlated to alterations associated to a bad outcome (RUNX1 mutations; and SRSF2, U2AF1, SF3B1, ASXL1 and BCOR mutations occurring frequently in MDS and MPN). Consistently, a high AKT3 expression level appears as a very strong predictor of poor survival. Curiously, although modestly varying among AML samples, a high AKT1 expression shows in contrast as a strong predictor of a better patient outcome. These data suggest that AKT3 and AKT1 expressions have strong, yet opposite, prognostic values., (© 2024. The Author(s).)
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- 2024
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48. Genome-resolved metagenomics of Venice Lagoon surface sediment bacteria reveals high biosynthetic potential and metabolic plasticity as successful strategies in an impacted environment.
- Author
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Banchi E, Corre E, Del Negro P, Celussi M, and Malfatti F
- Abstract
Bacteria living in sediments play essential roles in marine ecosystems and deeper insights into the ecology and biogeochemistry of these largely unexplored organisms can be obtained from 'omics' approaches. Here, we characterized metagenome-assembled-genomes (MAGs) from the surface sediment microbes of the Venice Lagoon (northern Adriatic Sea) in distinct sub-basins exposed to various natural and anthropogenic pressures. MAGs were explored for biodiversity, major marine metabolic processes, anthropogenic activity-related functions, adaptations at the microscale, and biosynthetic gene clusters. Starting from 126 MAGs, a non-redundant dataset of 58 was compiled, the majority of which (35) belonged to (Alpha- and Gamma-) Proteobacteria. Within the broad microbial metabolic repertoire (including C, N, and S metabolisms) the potential to live without oxygen emerged as one of the most important features. Mixotrophy was also found as a successful lifestyle. Cluster analysis showed that different MAGs encoded the same metabolic patterns (e.g . , C fixation, sulfate oxidation) thus suggesting metabolic redundancy. Antibiotic and toxic compounds resistance genes were coupled, a condition that could promote the spreading of these genetic traits. MAGs showed a high biosynthetic potential related to antimicrobial and biotechnological classes and to organism defense and interactions as well as adaptive strategies for micronutrient uptake and cellular detoxification. Our results highlighted that bacteria living in an impacted environment, such as the surface sediments of the Venice Lagoon, may benefit from metabolic plasticity as well as from the synthesis of a wide array of secondary metabolites, promoting ecosystem resilience and stability toward environmental pressures., Supplementary Information: The online version contains supplementary material available at 10.1007/s42995-023-00192-z., Competing Interests: Conflict of interestThe authors declare no conflict of interest., (© The Author(s) 2023.)
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- 2023
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49. Comparative Population Transcriptomics Provide New Insight into the Evolutionary History and Adaptive Potential of World Ocean Krill.
- Author
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Choquet M, Lenner F, Cocco A, Toullec G, Corre E, Toullec JY, and Wallberg A
- Subjects
- Animals, Phylogeny, Transcriptome, Gene Expression Profiling, Genomics, Antarctic Regions, Euphausiacea genetics
- Abstract
Genetic variation is instrumental for adaptation to changing environments but it is unclear how it is structured and contributes to adaptation in pelagic species lacking clear barriers to gene flow. Here, we applied comparative genomics to extensive transcriptome datasets from 20 krill species collected across the Atlantic, Indian, Pacific, and Southern Oceans. We compared genetic variation both within and between species to elucidate their evolutionary history and genomic bases of adaptation. We resolved phylogenetic interrelationships and uncovered genomic evidence to elevate the cryptic Euphausia similis var. armata into species. Levels of genetic variation and rates of adaptive protein evolution vary widely. Species endemic to the cold Southern Ocean, such as the Antarctic krill Euphausia superba, showed less genetic variation and lower evolutionary rates than other species. This could suggest a low adaptive potential to rapid climate change. We uncovered hundreds of candidate genes with signatures of adaptive evolution among Antarctic Euphausia but did not observe strong evidence of adaptive convergence with the predominantly Arctic Thysanoessa. We instead identified candidates for cold-adaptation that have also been detected in Antarctic fish, including genes that govern thermal reception such as TrpA1. Our results suggest parallel genetic responses to similar selection pressures across Antarctic taxa and provide new insights into the adaptive potential of important zooplankton already affected by climate change., Competing Interests: Conflict of interest statement. None declared., (© The Author(s) 2023. Published by Oxford University Press on behalf of Society for Molecular Biology and Evolution.)
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- 2023
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50. The Rhodoexplorer Platform for Red Algal Genomics and Whole-Genome Assemblies for Several Gracilaria Species.
- Author
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Lipinska AP, Krueger-Hadfield SA, Godfroy O, Dittami SM, Ayres-Ostrock L, Bonthond G, Brillet-Guéguen L, Coelho S, Corre E, Cossard G, Destombe C, Epperlein P, Faugeron S, Ficko-Blean E, Beltrán J, Lavaut E, Le Bars A, Marchi F, Mauger S, Michel G, Potin P, Scornet D, Sotka EE, Weinberger F, Cabral de Oliveira M, Guillemin ML, Plastino EM, and Valero M
- Subjects
- Ecosystem, Genomics, Genome, Gracilaria genetics, Rhodophyta genetics
- Abstract
Macroalgal (seaweed) genomic resources are generally lacking as compared with other eukaryotic taxa, and this is particularly true in the red algae (Rhodophyta). Understanding red algal genomes is critical to understanding eukaryotic evolution given that red algal genes are spread across eukaryotic lineages from secondary endosymbiosis and red algae diverged early in the Archaeplastids. The Gracilariales is a highly diverse and widely distributed order including species that can serve as ecosystem engineers in intertidal habitats and several notorious introduced species. The genus Gracilaria is cultivated worldwide, in part for its production of agar and other bioactive compounds with downstream pharmaceutical and industrial applications. This genus is also emerging as a model for algal evolutionary ecology. Here, we report new whole-genome assemblies for two species (Gracilaria chilensis and Gracilaria gracilis), a draft genome assembly of Gracilaria caudata, and genome annotation of the previously published Gracilaria vermiculophylla genome. To facilitate accessibility and comparative analysis, we integrated these data in a newly created web-based portal dedicated to red algal genomics (https://rhodoexplorer.sb-roscoff.fr). These genomes will provide a resource for understanding algal biology and, more broadly, eukaryotic evolution., (© The Author(s) 2023. Published by Oxford University Press on behalf of Society for Molecular Biology and Evolution.)
- Published
- 2023
- Full Text
- View/download PDF
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