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Continuous consumption combined with incomplete removal during wastewater treatment means residues of psychoactive substances (licit drugs, medications of abuse and illicit drugs) are constantly introduced into the aquatic environment, where they have the potential to affect non-target organisms. In this study, 17 drug residues of psychoactive substances were determined in wastewater influent, effluent and in receiving rivers of six Slovene municipal wastewater treatment plants employing different treatment technologies. Variations in removal efficiencies (REs) during spring, summer and winter were explored, and ecotoxic effects were evaluated using in silico (Ecological Structure-Activity Relationships software-ECOSAR) and in vivo (algal growth inhibition test) methods. Drug residues were detected in influent and effluent in the ng/L to μg/L range. In receiving rivers, biomarkers were in the ng/L range, and there was good agreement between measured and predicted concentrations. On average, REs were highest for nicotine, 11-nor-9-carboxy-∆9-tetrahydrocannabinol (THC-COOH), cocaine residues, and amphetamine (>90 %) and lowest for methadone residues (<30 %). REs were comparable between treatments involving activated sludge and membrane bioreactors, while the moving biofilm bed reactor (MBBR) removed cotinine, cocaine, and benzoylecgonine to a lesser extent. Accordingly, higher levels of nicotine and cocaine residues were detected in river water receiving MBBR discharge. Although there were seasonal variations in REs and levels of drug residues in receiving rivers, no general pattern could be observed. No significant inhibition of algal growth (Chlamydomonas reinhardtii) was observed for the tested compounds (1 mg/L) during 72 h and 240 h of exposure, although effects on aquatic plants were predicted in silico. In addition, environmental risk assessment revealed that levels of nicotine, methadone, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP), morphine, and 3,4-methylenedioxymethamphetamine (MDMA) pose a risk to aquatic organisms. Since nicotine and EDDP can have acute and chronic effects, the authors support regular monitoring of receiving surface waters, followed up by regulatory actions., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)

This study reports the occurrence of eight bisphenols (BPs): bisphenol AF (BPAF), bisphenol AP (BPAP), bisphenol B (BPB), bisphenol C (BPC), bisphenol E (BPE), bisphenol F (BPF), bisphenol S (BPS) and bisphenol Z (BPZ) in wastewaters (WWs). Sample preparation involved pre-concentration with SPE cartridges (Oasis HLB), followed by derivatization using N-(tert-butyldimethylsilyl)-N-methyltrifluoroacetamide with 1% tert-butyldimethylchlorosilane. Chemical analysis was based on gas chromatography-mass spectrometry. A validated method with limits of detection (LODs) at ngL -1 range was applied to WWs collected at five Slovene wastewater treatment plants (WWTPs) and WW inflows from industrial, commercial and residential sources entering the sewerage systems of two catchments (Domžale-Kamnik (DK) and Ljubljana (LJ)). The presence of all BPs was confirmed in three inflows in DK and two inflows in the LJ catchments. High cumulative concentrations of all BPs were determined in WW from food processing facilities (LJ: 3030ngL -1 and DK: 599ngL -1 ). A high detection frequency was observed in the WW from two textile cleaning companies (6 BPs for LJ and 8 BPs for DK). The analysis of WW from WWTPs revealed that only BPF (36.7ngL -1 ) and BPS (40.6ngL -1 ) were >LODs in the influents, whereas other BPs were detected also in the effluents. BPZ was found in the highest concentration (403ngL -1 at WWTP-DK). WW collected at this WWTP also contained the highest amount of BPE (238ngL -1 ). Although BPs removal could not be directly compared between the WWTPs, with the exception of BPAP and BPB in the case of two smaller WWTPs (6.39%-43.2%) bisphenols were in general highly removed (≥96.2%). Finally, levels of BPC>LOD are reported for first time (WWTP in the DK catchment: 1.01ngL -1 -11.8ngL -1 ; LJ inflow from food processing plant up to 2560ngL -1 )., (Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.)