Search

Your search keyword '"Chiang, Chien-Min"' showing total 27 results
Start Over Author "Chiang, Chien-Min" Publication Year Range Last 50 years
27 results on '"Chiang, Chien-Min"'

9. Crystal structure of cardiotoxin V from Taiwan cobra venom: pH-dependent conformational change and a novel membrane-binding motif identified in the three-finger loops of P-type cardiotoxin

Author

Sun, Yuh-Ju, Wu, Wen-guey, Chiang, Chien-Min, Hsin, A-Yen, and Hsiao, Chwan-Deng

Subjects
Toxins -- Research, Cobras -- Research, X-ray crystallography -- Usage, Nuclear magnetic resonance spectroscopy -- Usage, Conformational analysis -- Usage, Protein binding -- Analysis, Biological sciences, Chemistry
Abstract

X-ray diffraction and nuclear magnetic resonance (NMR) spectroscopy were done to characterize the structure of the cardiotoxin V from Taiwan cobra (CTX A5), a P-type CTX. The results showed that CTX A5 exhibits a dimeric assembly at pH 8.5. There were, however, differences in the data obtained from the two analytical methods which are localized at two functionally important regions in loops I and II. Such disparities were attributed to the difference in the protonation state of His4 at different pH which results to a reorientation of the His4 imidazole ring and to the hydrophobic contact between CTX dimers in the crystal packing as well as to the interaction of water molecules with amino acid residues in the loop II region.

Published
1997

10. The role of acidic amino acid residues in the structural stability of snake cardiotoxins

Author

Chiang, Chien-Min, Chang, Shou-Lin, Lin, Hai-jui, and Wu, Wen-guey

Subjects
Amino acids -- Analysis, Hydrogen-ion concentration -- Physiological aspects, Toxins -- Analysis, Chemical structure -- Analysis, Biological sciences, Chemistry
Abstract

Acidic pH disturbs the structural stability of the cardiotoxin V from the venom of the cobra Naja naja atra (CTX A5), leading to suppression of membrane-associated activity of the molecule. However, it fails to alter the overall beta-sheet of the molecule. The structural stability changes in CTX A5 occur at pH2 and pH4. The amino acid residue in the molecule, Glu-17, is involved in its structural stability. This residue disturbs the interaction between two other residues, Lys-2 and Asp-59, thus disturbing the overall stability of the beta-sheet in the presence of a denaturing reagent.

Published
1996

11. Conformational change and inactivation of membrane phospholipid-related activity of cardiotoxin V from Taiwan cobra venom at acidic pH

Author

Chiang, Chien-Min, Chien, Kun-Yi, Lin, Hai-jui, Lin, Ji-Fu, Yeh, Hsien-Chi, Ho, Pei-li, and Wu, Wen-guey

Subjects
Venom -- Physiological aspects, Toxins -- Physiological aspects, Phospholipids -- Analysis, Cooperative binding (Biochemistry) -- Research, Biological sciences, Chemistry
Abstract

Cardiotoxin V from Naja naja atra (CTX A5) shows pH-dependence while binding to phosphatidylcholine membrane with pK(sub a) value around 6.0. Proton NMR analysis indicates less CTX A5 relation to membranes at low pH, due to protonation of His-4 near the CTX loop I region's membrane binding site. Due to the influence of pH on pK(sub a), the protonation of His-4 residue also leads to local conformational change in the loop I region, and other amino acid residues in the protein's structural core receive this effect.

Published
1996

24. Crystal Structures of Aspergillus japonicus Fructosyltransferase Complex with Donor/Acceptor Substrates Reveal Complete Subsites in the Active Site for Catalysis

Author

Chuankhayan, Phimonphan, primary, Hsieh, Chih-Yu, additional, Huang, Yen-Chieh, additional, Hsieh, Yi-You, additional, Guan, Hong-Hsiang, additional, Hsieh, Yin-Cheng, additional, Tien, Yueh-Chu, additional, Chen, Chung-De, additional, Chiang, Chien-Min, additional, and Chen, Chun-Jung, additional

Published
2010
Full Text
View/download PDF

27. Inhibitory effect of a water extract from Pemphis acidula on melanogenesis in mouse B16 melanoma cells.

Author

Ding HY, Chang TS, Chiang CM, and Shou-Ku Tai S

Subjects
Animals, Cell Line, Tumor, Cell Survival drug effects, Gene Expression Regulation, Enzymologic, Melanins metabolism, Mice, Monophenol Monooxygenase antagonists & inhibitors, Monophenol Monooxygenase metabolism, Plant Bark chemistry, RNA, Messenger genetics, RNA, Messenger metabolism, Lythraceae chemistry, Plant Extracts chemistry, Plant Extracts pharmacology, Water chemistry
Abstract

The inhibitory effect of a water extract from Pemphis acidula on melanogenesis in mouse B16 melanoma cells was investigated. The results showed that the P. acidula extract (PAE) inhibited melanogenesis in 3-isobutyl-1-methylxanthin (IBMX)-stimulated B16 cells in a dose-dependent manner, with an IC50 value of 33.5 μg/ml. In addition, PAE also inhibited cellular tyrosinase activity. Moreover, western blot and real-time reverse transcriptase polymerase chain reaction (qRT-PCR) analyses respectively confirmed that PAE down-regulated levels of tyrosinase protein and its mRNA in IBMX-stimulated B16 cells. These results demonstrated that PAE inhibits melanogenesis of B16 cells by reducing tyrosinase gene expression. From the present study, PAE is proven to be a good candidate as a skin-whitening agent for treatment of skin hyperpigmentation.

Published
2011

Catalog

Books, media, physical & digital resources
See catalog results