Search

Your search keyword '"Charlène Clement"' showing total 2 results
Start Over Author "Charlène Clement" Publication Year Range Last 50 years
2 results on '"Charlène Clement"'

1. The fecal microbiota of piglets during weaning transition and its association with piglet growth across various farm environments.

Author

Diana Luise, Mathilde Le Sciellour, Arnaud Buchet, Rémi Resmond, Charlène Clement, Marie-Noelle Rossignol, Deborah Jardet, Olivier Zemb, Catherine Belloc, and Elodie Merlot

Subjects
Medicine, Science
Abstract

This study describes the fecal microbiota from piglets reared in different living environments during the weaning transition, and presents the characteristics of microbiota associated with good growth of piglets after weaning. Fecal samples were collected pre- (d26) and post-weaning (d35) from 288 male piglets in 16 conventional indoor commercial farms located in the West of France. The changes one week after weaning on the most abundant microbial families was roughly the same in all farms: alpha diversity increased, the relative abundance of Bacteroidaceae (-61%), Christensenellaceae (-35%), Enterobacteriaceae (-42%), and Clostridiaceae (-32%) decreased, while the relative abundance of Prevotellaceae (+143%) and Lachnospiraceae (+21%) increased. Among all the collected samples, four enterotypes that were ubiquitous in all farms were identified. They could be discriminated by their respective relative abundances of Prevotella, Faecalibacterium, Roseburia, and Lachnospira, and likely corresponded to a gradual maturational shift from pre- to post-weaning microbiota. The rearing environment influenced the frequency of enterotypes, as well as the relative abundance of 6 families at d26 (including Christensenellaceae and Lactobacillaceae), and of 21 families at d35. In all farms, piglets showing the highest relative growth rate during the first three weeks after weaning, which were characterized as more robust, had a higher relative abundance of Bacteroidetes, a lower relative abundance of Proteobacteria, and showed a greater increase in Prevotella, Coprococcus, and Lachnospira in the post-weaning period. This study revealed the presence of ubiquitous enterotypes among the farms of this study, reflecting maturational stages of microbiota from a young suckling to an older cereal-eating profile. Despite significant variation in the microbial profile between farms, piglets whose growth after weaning was less disrupted were, those who had reached the more mature phenotype characterized by Prevotella the fastest.

Published
2021
Full Text
View/download PDF

2. Le microbiote du porcelet au sevrage : influence de l’environnement d’élevage, et corrélations avec la robustesse

Author

Elodie Merlot, Mathilde Le Sciellour, Diana Luise, Arnaud Buchet, Rémi Resmond, Charlène Clement, Olivier Zemb, Catherine Belloc, Physiologie, Environnement et Génétique pour l'Animal et les Systèmes d'Elevage [Rennes] (PEGASE), AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Distal, Alma Mater Studiorum Università di Bologna [Bologna] (UNIBO), Cooperl Innovation, Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Biologie, Epidémiologie et analyse de risque en Santé Animale (BIOEPAR), and Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
abundance, sevrage, weaning, [SDV]Life Sciences [q-bio], abondance, statut sanitaire, sucking pig, robustness, robustesse, antibiotique, taxonomie bactérienne, antibiotic, flore digestive, porcelet
Abstract

International audience; This study describes variability among farms of the response of microbiota to weaning and evaluates its relation to the robustness of piglets. Faecal samples and growth data were collected just before and seven days after weaning in 288 piglets from 16 commercial farms characterised by their pathogenic status and antimicrobial use. We observed in all farms the presence of four bacterial enterotypes (E1 to E4), differing notably in their abundance of Prevotella, Faecalibacterium, Roseburia, and Lachnospira, and corresponding to a gradual maturational shift from pre- to post-weaning microbiota. After weaning, of the 1 177 operational taxonomic units (OTU) contained in the dataset, 462 and 340 had their abundance affected (P < 0.05) by the use of antimicrobials and by the health status of the herd, respectively. Animals were then classified according to their relative average daily gain (rADG) from 26 to 48 days of age. The high rADG piglets had less Oscillospira (P < 0.05) and Campylobacter (P < 0.01), and more Prevotella (P < 0.01), Coprococcus (P < 0.01) and Lachnospira (P < 0.05) after weaning, which resembled the E4 enterotype. Thus, using animals from 16 farms, we identified four ubiquitous maturation enterotypes and found that piglets that reached enterotype E4 just after weaning might be more robust. Besides antimicrobial use, the general health status of the farm has a strong influence on the early post-weaning evolution of piglet microbiota.; Cette étude décrit la variabilité entre élevages de l’évolution du microbiote au moment du sevrage, et évalue la relation entre cette dernière et la robustesse des porcelets. Des échantillons fécaux et des enregistrements de croissance ont été collectés juste avant et sept jours après le sevrage pour 288 porcelets issus de 16 élevages commerciaux, caractérisés pour leur statut sanitaire et leur usage d’antibiotiques. Nous avons observé dans toutes les fermes la présence de quatre entérotypes bactériens (E1 à E4), différant notamment par leur abondance de Prevotella, Faecalibacterium, Roseburia, et Lachnospira, et correspondant à des stades de maturation graduelle pré- vers post-sevrage de la flore. Après le sevrage, des 1 177 unitéstaxonomiques (OTU) contenues dans le jeu de données, 462 et 340 voyaient leur abondance respectivement influencée par l’utilisation d’antibiotiques et par le statut sanitaire de l’élevage. Les animaux ont ensuite été classés selon leur gain de poids relatif (GMQr) calculé entre 26 et 48 jours d’âge. Les porcelets à haut GMQr avaient moins d’Oscillospira (P < 0,05) et Campylobacter (P < 0,01), et plus de Prevotella (P < 0,01), Coprococcus (P < 0,01) et Lachnospira (P < 0,05) après le sevrage, ce qui s’apparentait à l’entérotype E4. Ainsi, en utilisant des animaux provenant de 16 fermes, nous avons pu identifier quatreentérotypes maturationnels ubiquitaires, et supposé que les porcelets ayant atteint l’entérotype E4 juste après le sevrage pourraient être plus robustes. Hormis l’usage d’antibiotiques, le statut sanitaire global de l’élevage a une influence importante sur l’évolution précoce du microbiote après le sevrage.

Published
2020

Catalog

Books, media, physical & digital resources
See catalog results