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Your search keyword '"CHROMOBOX HOMOLOG 8"' showing total 3 results
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3 results on '"CHROMOBOX HOMOLOG 8"'

1. RETRACTED ARTICLE: Correlations between chromobox homolog 8 and key factors of epithelial–mesenchymal transition in hepatocellular carcinoma

Author

Wei Luo, Di Li, Shidong Zhang, Juan Kong, Xiaonian Zhu, Yuanyuan Fu, Chunhua Bei, and Shengkui Tan

Subjects
Cancer Research, Hepatocellular carcinoma, Biology, lcsh:RC254-282, Metastasis, 03 medical and health sciences, 0302 clinical medicine, CHROMOBOX HOMOLOG 8, Genetics, medicine, Epithelial–mesenchymal transition, lcsh:QH573-671, neoplasms, 030304 developmental biology, 0303 health sciences, Tissue microarray, Oncogene, lcsh:Cytology, medicine.disease, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, digestive system diseases, Key factors, Oncology, 030220 oncology & carcinogenesis, Cancer research, PRC1, Chromobox protein homolog 8, Epithelial mesenchymal transition
Abstract

Background Hepatocellular carcinoma (HCC) is one of the most common malignancies worldwide, especially in China, with high metastasis and poor prognosis. Recently, as the core component of the polycomb repressive complexes 1 (PRC1), chromobox protein homolog 8 (CBX8) is considered as an oncogene and prognostic marker in HCC. Methods A tissue microarray of 166 paired HCC and adjacent non-tumor samples were collected to identify the relationship between CBX8 and epithelial mesenchymal transition (EMT) associated proteins by Spearman correlation analysis. Knock-down of CBX8 in HCC cells was conducted to detect the biologic functions of CBX8 in HCC metastasis. Results We found out that CBX8 was over-expressed in HCC and its expression was closely related to the metastasis of HCC patients. In addition, knock-down of CBX8 was found to inhibit the invasion and migration ability of HCC cells. Moreover, there was a significant relationship between expression of CBX8 and EMT associated proteins both in HCC cells and tumor tissues. Conclusions Our results indicate that CBX8 promotes metastasis of HCC by inducing EMT process.

Published
2019

2. Retraction Note to: Correlations between chromobox homolog 8 and key factors of epithelial–mesenchymal transition in hepatocellular carcinoma

Author

Shengkui Tan, Juan Kong, Chunhua Bei, Yuanyuan Fu, Shidong Zhang, Wei Luo, Di Li, and Xiaonian Zhu

Subjects
Cancer Research, Tissue microarray, Oncogene, lcsh:Cytology, Biology, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, medicine.disease, lcsh:RC254-282, digestive system diseases, Metastasis, 03 medical and health sciences, Retraction Note, 0302 clinical medicine, Key factors, Oncology, 030220 oncology & carcinogenesis, Hepatocellular carcinoma, CHROMOBOX HOMOLOG 8, Genetics, medicine, Cancer research, Epithelial–mesenchymal transition, lcsh:QH573-671, PRC1, neoplasms
Abstract

Hepatocellular carcinoma (HCC) is one of the most common malignancies worldwide, especially in China, with high metastasis and poor prognosis. Recently, as the core component of the polycomb repressive complexes 1 (PRC1), chromobox protein homolog 8 (CBX8) is considered as an oncogene and prognostic marker in HCC. A tissue microarray of 166 paired HCC and adjacent non-tumor samples were collected to identify the relationship between CBX8 and epithelial mesenchymal transition (EMT) associated proteins by Spearman correlation analysis. Knock-down of CBX8 in HCC cells was conducted to detect the biologic functions of CBX8 in HCC metastasis. We found out that CBX8 was over-expressed in HCC and its expression was closely related to the metastasis of HCC patients. In addition, knock-down of CBX8 was found to inhibit the invasion and migration ability of HCC cells. Moreover, there was a significant relationship between expression of CBX8 and EMT associated proteins both in HCC cells and tumor tissues. Our results indicate that CBX8 promotes metastasis of HCC by inducing EMT process.

Published
2020

3. Investigation of the relationship between chromobox homolog 8 and nucleus pulposus cells degeneration in rat intervertebral disc.

Author

Zhou, Xu, Zhang, Hai-Long, Gu, Guang-Fei, Ding, Yue, Jia, Jian-Bo, Fu, Qing-Song, and He, Shi-Sheng

Abstract

Here, we aimed to investigate the expression of chromobox homolog 8 (CBX8) in nucleus pulposus (NP) cells from rat intervertebral disc (IVD) and its function in DNA damage and repair. NP cells were isolated from healthy rat IVD for immunohistochemistry staining. Small interfering RNA (siRNA) of CBX8 was applied for gene silencing, and reverse transcriptase-polymerase chain reaction (RT-PCR) was performed to determine mRNA levels of CBX8, type II collagen, and proteoglycans. Cell proliferation and cell cycle were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, colony-forming assay, and flow cytometry. Hydrogen peroxide (HO) was added to simulate DNA oxidative damage, and expression of CBX8 was examined using RT-PCR and Western blot. After five passages, mRNA levels of type II collagen and proteoglycans decreased but that of CBX8 increased. When CBX8 was silenced by siRNA, the expressions of CBX8, type II collagen and proteoglycans declined, and the cell growth was inhibited. Besides, cell cycle was slowed down as most cells were arrest in G0/G1 phase. Furthermore, CBX8 expression went up responding to DNA oxidative damage caused by HO. The data indicated that CBX8 plays important roles in cell proliferation and DNA damage. Cell proliferation and cell cycle were stimulated by CBX8, which may be associated with INK4A-ARF pathway. Moreover, CBX8 plays a role in DNA damage which made it a potential gene therapy target for treatment of disc degeneration. [ABSTRACT FROM AUTHOR]

Published
2013
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