1. Alternative metabolic routes in channeling xylose to cordycepin production of Cordyceps militaris identified by comparative transcriptome analysis
- Author
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Boontariga Wongsa, Kobkul Laoteng, Nachon Raethong, Wanwipa Vongsangnak, Jirasak Wong-ekkabut, and Pramote Chumnanpuen
- Subjects
0106 biological sciences ,Glucuronate ,Pentose ,Biology ,Xylose ,Real-Time Polymerase Chain Reaction ,01 natural sciences ,Transcriptome ,03 medical and health sciences ,chemistry.chemical_compound ,Biosynthesis ,Gene Expression Regulation, Fungal ,Cordyceps militaris ,Genetics ,030304 developmental biology ,chemistry.chemical_classification ,0303 health sciences ,Deoxyadenosines ,Cordycepin ,Gene Expression Profiling ,biology.organism_classification ,chemistry ,Biochemistry ,Cordyceps ,Glycine ,010606 plant biology & botany - Abstract
The responsive mechanism of C. militaris TBRC7358 on xylose utilization was investigated by comparative analysis of transcriptomes, growth kinetics and cordycepin productions. The result showed that the culture grown on xylose exhibited high production yield of cordycepin on dry biomass. Comparing xylose to other carbon sources, a set of significantly up-regulated genes in xylose were enriched in pentose and glucuronate interconversion, and cordycepin biosynthesis. After validating up-regulated genes using quantitative real-time PCR, interestingly, putative alternative 3'-AMP-associated metabolic route on cordycepin biosynthesis was identified. Through reporter metabolites analysis of C. militaris, significant metabolites (e.g., AMP, glycine and L-glutamate) were identified guiding involvement of growth and cordycepin production. These findings suggested that there was a cooperative mechanism in transcriptional control of the supplying precursors pool directed towards the cordycepin biosynthesis through main and putative alternative metabolic routes for leverage of cell growth and cordycepin production on xylose of C. militaris strain TBRC7358.
- Published
- 2020
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