544 results on '"Boldrini R"'
Search Results
2. Clinical and ultrastructural spectrum of diffuse lung disease associated with surfactant protein C mutations
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Peca, D, Boldrini, R, Johannson, J, Shieh, JT, Citti, A, Petrini, S, Salerno, T, Cazzato, S, Testa, R, Messina, F, Onofri, A, Cenacchi, G, Westermark, P, Ullman, N, Cogo, P, Cutrera, R, and Danhaive, O
- Subjects
Genetics ,Clinical Sciences ,Genetics & Heredity - Abstract
Genetic defects of surfactant metabolism are associated with a broad range of clinical manifestations, from neonatal respiratory distress syndrome to adult interstitial lung disease. Early therapies may improve symptoms but diagnosis is often delayed owing to phenotype and genotype variability. Our objective was to characterize the cellular/ultrastructural correlates of surfactant protein C (SP-C) mutations in children with idiopathic diffuse lung diseases. We sequenced SFTPC-the gene encoding SP-C-SFTPB and ABCA3, and analyzed morphology, ultrastructure and SP expression in lung tissue when available. We identified eight subjects who were heterozygous for SP-C mutations. Median age at onset and clinical course were variable. None of the mutations were located in the mature peptide-encoding region, but were either in the pro-protein BRICHOS or linker C-terminal domains. Although lung morphology was similar to other genetic surfactant metabolism disorders, electron microscopy studies showed specific anomalies, suggesting surfactant homeostasis disruption, plus trafficking defects in the four subjects with linker domain mutation and protein misfolding in the single BRICHOS mutation carrier in whom material was available. Immunolabeling studies showed increased proSP-C staining in all cases. In two cases, amyloid deposits could be identified. Immunochemistry and ultrastructural studies may be useful for diagnostic purposes and for genotype interpretation.
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- 2015
3. Il moral distress nella professione di assistente sociale
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Bensi, S, Barsanti, S, Boldrini, R, Colombini, G, Sommati, V, Pardini, E, Bensi, S, Barsanti, S, Boldrini, R, Colombini, G, Sommati, V, and Pardini, E
- Published
- 2023
4. The first SARS-CoV-2 wave among pregnant women in Italy: results from a prospective population-based study
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Donati, S, Corsi, E, Maraschini, A, Salvatore, M, Baltaro, F, Boldrini, R, Bonassisa, S, Brunelli, R, Cagnacci, A, Casucci, P, Cataneo, I, Cetin, I, de Ambrosi, E, Manso, M, Fabiani, M, Fieni, S, Franchi, M, Iurlaro, E, Leo, L, Liberati, M, Sacchi, L, Livio, S, Locci, M, Marozio, L, Martini, C, Maso, G, Mecacci, F, Meloni, A, Mignuoli, A, Moresi, S, Patane, L, Perotti, F, Perrone, E, Prefumo, F, Ramenghi, L, Rusciani, R, Savasi, V, Schettini, S, Simeone, D, Soligo, M, Steinkasserer, M, Tateo, S, Ternelli, G, Tironi, R, Trojano, V, Vergani, P, Donati S., Corsi E., Maraschini A., Salvatore M. A., Baltaro F., Boldrini R., Bonassisa S., Brunelli R., Cagnacci A., Casucci P., Cataneo I., Cetin I., de Ambrosi E., Manso M. D., Fabiani M., Fieni S., Franchi M. P., Iurlaro E., Leo L., Liberati M., Sacchi L. L., Livio S., Locci M., Marozio L., Martini C., Maso G., Mecacci F., Meloni A., Mignuoli A. D., Moresi S., Patane L., Perotti F., Perrone E., Prefumo F., Ramenghi L., Rusciani R., Savasi V., Schettini S. C. A., Simeone D., Soligo M., Steinkasserer M., Tateo S., Ternelli G., Tironi R., Trojano V., Vergani P., Donati, S, Corsi, E, Maraschini, A, Salvatore, M, Baltaro, F, Boldrini, R, Bonassisa, S, Brunelli, R, Cagnacci, A, Casucci, P, Cataneo, I, Cetin, I, de Ambrosi, E, Manso, M, Fabiani, M, Fieni, S, Franchi, M, Iurlaro, E, Leo, L, Liberati, M, Sacchi, L, Livio, S, Locci, M, Marozio, L, Martini, C, Maso, G, Mecacci, F, Meloni, A, Mignuoli, A, Moresi, S, Patane, L, Perotti, F, Perrone, E, Prefumo, F, Ramenghi, L, Rusciani, R, Savasi, V, Schettini, S, Simeone, D, Soligo, M, Steinkasserer, M, Tateo, S, Ternelli, G, Tironi, R, Trojano, V, Vergani, P, Donati S., Corsi E., Maraschini A., Salvatore M. A., Baltaro F., Boldrini R., Bonassisa S., Brunelli R., Cagnacci A., Casucci P., Cataneo I., Cetin I., de Ambrosi E., Manso M. D., Fabiani M., Fieni S., Franchi M. P., Iurlaro E., Leo L., Liberati M., Sacchi L. L., Livio S., Locci M., Marozio L., Martini C., Maso G., Mecacci F., Meloni A., Mignuoli A. D., Moresi S., Patane L., Perotti F., Perrone E., Prefumo F., Ramenghi L., Rusciani R., Savasi V., Schettini S. C. A., Simeone D., Soligo M., Steinkasserer M., Tateo S., Ternelli G., Tironi R., Trojano V., and Vergani P.
- Abstract
Introduction. This study aimed to estimate the incidence of SARS-CoV-2 infection among pregnant women during the first pandemic wave in Italy, and to describe CO-VID-19 disease characteristics and maternal and perinatal outcomes. Materials and methods. National population-based prospective cohort study collecting information on women with SARS-CoV-2 diagnosis, confirmed within 7 days from hospital admission. Results. The national SARS-CoV-2 rate was 6.04 per 1,000 births (95% CI 5.62-6.49) among pregnant women and 7.54 (95% CI 7.47-7.61) among women in reproductive age. 72.1% of the cohort developed mild COVID-19 disease without pneumonia nor need for ventilatory support. Severe disease was significantly associated with women’s previous comorbidities (OR 2.55; 95% CI 0.98-6.90), obesity (OR 4.76; 95% CI 1.79-12.66) and citizenship from High Migration Pressure Countries (OR 3.43; 95% CI 1.27-9.25). Conclusions. During the first pandemic wave in Italy, the SARS-CoV-2 rate among pregnant women was lower compared to that detected among women of reproductive age, and risks of severe COVID-19 disease and adverse maternal and perinatal outcomes were rare.
- Published
- 2021
5. Novel PNPLA1 mutations in two Italian siblings with autosomal recessive congenital ichthyosis
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Diociaiuti, A., Pisaneschi, E., Zambruno, G., Angioni, A., Novelli, A., Boldrini, R., and El Hachem, M.
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- 2018
- Full Text
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6. The first SARS-CoV-2 wave among pregnant women in Italy: results from a prospective population-based study
- Author
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Donati S., Corsi E., Maraschini A., Salvatore M. A., Baltaro F., Boldrini R., Bonassisa S., Brunelli R., Cagnacci A., Casucci P., Cataneo I., Cetin I., de Ambrosi E., Manso M. D., Fabiani M., Fieni S., Franchi M. P., Iurlaro E., Leo L., Liberati M., Sacchi L. L., Livio S., Locci M., Marozio L., Martini C., Maso G., Mecacci F., Meloni A., Mignuoli A. D., Moresi S., Patane L., Perotti F., Perrone E., Prefumo F., Ramenghi L., Rusciani R., Savasi V., Schettini S. C. A., Simeone D., Soligo M., Steinkasserer M., Tateo S., Ternelli G., Tironi R., Trojano V., Vergani P., Donati, S., Corsi, E., Maraschini, A., Salvatore, M. A., Baltaro, F., Boldrini, R., Bonassisa, S., Brunelli, R., Cagnacci, A., Casucci, P., Cataneo, I., Cetin, I., de Ambrosi, E., Manso, M. D., Fabiani, M., Fieni, S., Franchi, M. P., Iurlaro, E., Leo, L., Liberati, M., Sacchi, L. L., Livio, S., Locci, M., Marozio, L., Martini, C., Maso, G., Mecacci, F., Meloni, A., Mignuoli, A. D., Moresi, S., Patane, L., Perotti, F., Perrone, E., Prefumo, F., Ramenghi, L., Rusciani, R., Savasi, V., Schettini, S. C. A., Simeone, D., Soligo, M., Steinkasserer, M., Tateo, S., Ternelli, G., Tironi, R., Trojano, V., Vergani, P., Donati, S, Corsi, E, Maraschini, A, Salvatore, M, Baltaro, F, Boldrini, R, Bonassisa, S, Brunelli, R, Cagnacci, A, Casucci, P, Cataneo, I, Cetin, I, de Ambrosi, E, Manso, M, Fabiani, M, Fieni, S, Franchi, M, Iurlaro, E, Leo, L, Liberati, M, Sacchi, L, Livio, S, Locci, M, Marozio, L, Martini, C, Maso, G, Mecacci, F, Meloni, A, Mignuoli, A, Moresi, S, Patane, L, Perotti, F, Perrone, E, Prefumo, F, Ramenghi, L, Rusciani, R, Savasi, V, Schettini, S, Simeone, D, Soligo, M, Steinkasserer, M, Tateo, S, Ternelli, G, Tironi, R, Trojano, V, and Vergani, P
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SARS-CoV-2 ,Infectious ,Cohort studies ,Italy ,Pregnancy outcome ,COVID-19 Testing ,Citizenship ,Female ,Humans ,Pregnancy ,Pregnant Women ,Prospective Studies ,COVID-19 ,Pregnancy Complications, Infectious ,Pregnancy Complications ,Cohort studie - Abstract
Introduction. This study aimed to estimate the incidence of SARS-CoV-2 infection among pregnant women during the first pandemic wave in Italy, and to describe CO-VID-19 disease characteristics and maternal and perinatal outcomes. Materials and methods. National population-based prospective cohort study collecting information on women with SARS-CoV-2 diagnosis, confirmed within 7 days from hospital admission. Results. The national SARS-CoV-2 rate was 6.04 per 1,000 births (95% CI 5.62-6.49) among pregnant women and 7.54 (95% CI 7.47-7.61) among women in reproductive age. 72.1% of the cohort developed mild COVID-19 disease without pneumonia nor need for ventilatory support. Severe disease was significantly associated with women’s previous comorbidities (OR 2.55; 95% CI 0.98-6.90), obesity (OR 4.76; 95% CI 1.79-12.66) and citizenship from High Migration Pressure Countries (OR 3.43; 95% CI 1.27-9.25). Conclusions. During the first pandemic wave in Italy, the SARS-CoV-2 rate among pregnant women was lower compared to that detected among women of reproductive age, and risks of severe COVID-19 disease and adverse maternal and perinatal outcomes were rare.
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- 2021
7. The Polycomb group (PcG) protein EZH2 supports the survival of PAX3-FOXO1 alveolar rhabdomyosarcoma by repressing FBXO32 (Atrogin1/MAFbx)
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Ciarapica, R, De Salvo, M, Carcarino, E, Bracaglia, G, Adesso, L, Leoncini, P P, DallʼAgnese, A, Walters, Z S, Verginelli, F, De Sio, L, Boldrini, R, Inserra, A, Bisogno, G, Rosolen, A, Alaggio, R, Ferrari, A, Collini, P, Locatelli, M, Stifani, S, Screpanti, I, Rutella, S, Yu, Q, Marquez, V E, Shipley, J, Valente, S, Mai, A, Miele, L, Puri, P L, Locatelli, F, Palacios, D, and Rota, R
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- 2014
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8. A truncating mutation in the laminin-332α chain highlights the role of the LG45 proteolytic domain in regulating keratinocyte adhesion and migration
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Di Zenzo, G., El Hachem, M., Diociaiuti, A., Boldrini, R., Calabresi, V., Cianfarani, F., Fortugno, P., Piccinni, E., Zambruno, G., and Castiglia, D.
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- 2014
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9. In vivo bladder regeneration using small intestinal submucosa: experimental study
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Caione, P., Capozza, N., Zavaglia, D., Palombaro, G., and Boldrini, R.
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- 2006
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10. Cellular and gene signatures of tumor-infiltrating dendritic cells and natural-killer cells predict prognosis of neuroblastoma
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Melaiu, O., Chierici, M., Lucarini, V., Jurman, G., Conti, L. A., De Vito, R., Boldrini, R., Cifaldi, L., Castellano, A., Furlanello, C., Barnaba, V., Locatelli, Franco, Fruci, D., Locatelli F. (ORCID:0000-0002-7976-3654), Melaiu, O., Chierici, M., Lucarini, V., Jurman, G., Conti, L. A., De Vito, R., Boldrini, R., Cifaldi, L., Castellano, A., Furlanello, C., Barnaba, V., Locatelli, Franco, Fruci, D., and Locatelli F. (ORCID:0000-0002-7976-3654)
- Abstract
Tumor-infiltrating lymphocytes play an essential role in improving clinical outcome of neuroblastoma (NB) patients, but their relationship with other tumor-infiltrating immune cells in the T cell-inflamed tumors remains poorly investigated. Here we show that dendritic cells (DCs) and natural killer (NK) cells are positively correlated with T-cell infiltration in human NB, both at transcriptional and protein levels, and associate with a favorable prognosis. Multiplex imaging displays DC/NK/T cell conjugates in the tumor microenvironment of low-risk NB. Remarkably, this connection is further strengthened by the identification of gene signatures related to DCs and NK cells able to predict survival of NB patients and strongly correlate with the expression of PD-1 and PD-L1. In summary, our findings unveil a key prognostic role of DCs and NK cells and indicate their related gene signatures as promising tools for the identification of clinical biomarkers to better define risk stratification and survival of NB patients.
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- 2020
11. Immunofluorescence mapping, electron microscopy and genetics in the diagnosis and sub‐classification of inherited epidermolysis bullosa: a single‐centre retrospective comparative study of 87 cases with long‐term follow‐up
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Rossi, S., primary, Castiglia, D., additional, Pisaneschi, E., additional, Diociaiuti, A., additional, Stracuzzi, A., additional, Cesario, C., additional, Mariani, R., additional, Floriddia, G., additional, Zambruno, G., additional, Boldrini, R., additional, Abeni, D., additional, Novelli, A., additional, Alaggio, R., additional, and El Hachem, M., additional
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- 2021
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12. ADAR2-editing activity inhibits glioblastoma growth through the modulation of the CDC14B/Skp2/p21/p27 axis
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Galeano, F, Rossetti, C, Tomaselli, S, Cifaldi, L, Lezzerini, M, Pezzullo, M, Boldrini, R, Massimi, L, Di Rocco, C M, Locatelli, F, and Gallo, A
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- 2013
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13. Pediatric chronic intestinal failure in italy: Report from the 2016 survey on behalf of Italian society for gastroenterology, hepatology and nutrition (SIGENP)
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Diamanti, A, Capriati, T, Gandullia, P, Di Leo, G, Lezo, A, Lacitignola, L, Spagnuolo, M, Gatti, S, D'Antiga, L, Verlato, G, Roggero, P, Amarri, S, Baldassarre, M, Cirillo, F, Elia, D, Boldrini, R, Campanozzi, A, Catassi, C, Aloi, M, Romano, C, Candusso, M, Cecchi, N, Bellini, T, Tyndall, E, Fusaro, F, Caldaro, T, Alberti, D, Gamba, P, Lima, M, Bagolan, P, De Ville de Goyet, J, Dall'Oglio, L, Spada, M, Grandi, F, Diamanti A, Capriati T, Gandullia P, Di Leo G, Lezo A, Lacitignola L, Spagnuolo MI, Gatti S, D'Antiga L, Verlato G, Roggero P, Amarri S, Baldassarre ME, Cirillo F, Elia D, Boldrini R, Campanozzi A, Catassi C, Aloi M, Romano C, Candusso M, Cecchi N, Bellini T, Tyndall E, Fusaro F, Caldaro T, Alberti D, Gamba P, Lima M, Bagolan P, De Ville de Goyet J, Dall'Oglio L, Spada M, Grandi F., Diamanti, A, Capriati, T, Gandullia, P, Di Leo, G, Lezo, A, Lacitignola, L, Spagnuolo, M, Gatti, S, D'Antiga, L, Verlato, G, Roggero, P, Amarri, S, Baldassarre, M, Cirillo, F, Elia, D, Boldrini, R, Campanozzi, A, Catassi, C, Aloi, M, Romano, C, Candusso, M, Cecchi, N, Bellini, T, Tyndall, E, Fusaro, F, Caldaro, T, Alberti, D, Gamba, P, Lima, M, Bagolan, P, De Ville de Goyet, J, Dall'Oglio, L, Spada, M, Grandi, F, Diamanti A, Capriati T, Gandullia P, Di Leo G, Lezo A, Lacitignola L, Spagnuolo MI, Gatti S, D'Antiga L, Verlato G, Roggero P, Amarri S, Baldassarre ME, Cirillo F, Elia D, Boldrini R, Campanozzi A, Catassi C, Aloi M, Romano C, Candusso M, Cecchi N, Bellini T, Tyndall E, Fusaro F, Caldaro T, Alberti D, Gamba P, Lima M, Bagolan P, De Ville de Goyet J, Dall'Oglio L, Spada M, and Grandi F.
- Abstract
Background: Intestinal failure (IF) is the reduction in functioning gut mass below the minimal level necessary for adequate digestion and absorption of nutrients and fluids for weight maintenance in adults or for growth in children. There is a paucity of epidemiologic data on pediatric IF. The purpose of this study was to determine the prevalence, incidence, regional distribution and underlying diagnosis of pediatric chronic IF (CIF) requiring home parenteral nutrition (HPN) in Italy. Methods: Local investigators were selected in 19 Italian centers either of reference for pediatric HPN or having pediatric gastroenterologists or surgeons on staff and already collaborating with the Italian Society for Pediatric Gastroenterology, Hepatology and Nutrition with regard to IF. Data requested in this survey for children at home on Parenteral Nutrition (PN) on 1 December 2016 included patient initials, year of birth, gender, family’s place of residence and underlying diagnosis determining IF. Results: We recorded 145 CIF patients on HPN aged ≤19 years. The overall prevalence was 14.12/million inhabitants (95% CI: 9.20–18.93); the overall incidence was 1.41/million inhabitant years (95% CI: 0.53–2.20). Conclusion: Our survey provides new epidemiological data on pediatric CIF in Italy; these data may be quantitatively useful in developing IF care strategy plans in all developed countries.
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- 2017
14. Camelobaetidius metae Salinas-Jimenez & Boldrini & Osorio-Ramirez & Caro & Rojas-Pe��a 2019, sp. nov
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Salinas-Jimenez, Luis Gonzalo, Boldrini, R., Osorio-Ramirez, Diana Paola, Caro, Clara Ines, and Rojas-Pe��a, Jose Ismael
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Camelobaetidius ,Insecta ,Arthropoda ,Camelobaetidius metae ,Animalia ,Biodiversity ,Ephemeroptera ,Baetidae ,Taxonomy - Abstract
Camelobaetidius metae Salinas-Jimenez & Boldrini, sp. nov. (Figs. 1���11) Diagnosis. Nymphs: 1) labrum narrowly rounded anteriorly (Fig. 2); 2) incisors of right mandible with eleven denticles (Fig. 5a); 3) segment II of labial palp with a short rounded distomedial projection (Fig. 7); 4) gills absent at the bases of coxae; 5) outer margin of forefemur with a row of about 23 long, spine-like setae (Fig. 8); 6) tarsal claws with ten denticles (Fig. 8a); 7) posterior margin of tergum IV with truncate spines (Fig. 9); 8) terminal filament almost as long as cerci. Description. Nymph. Length of body: 3.5���4.0 mm; length of antennae: 1.0��� 1.2 mm; length of cerci: 1.0 mm; length of terminal filament: 0.8 mm. Head (Fig. 1). Light brown. Antennae light brown. Lateral branch of epicranial suture sinuous. Labrum (Fig. 2). Narrowly rounded anteriorly, broader than long. Length about 0.6�� maximum width; lateral and anterolateral margins with long, fine, bifid and pectinate setae; medially with fine, bifid and pectinate setae. Dorsally with three short, fine, simple setae scattered over basal area; dorsolateral arc of setae with two long, fine, apically pointed setae in lateral region. Hypopharynx (Fig. 3). Lingua shorter than superlingua, apex rounded, with tuft of short, fine, simple setae. Superlingua not expanded, with fine, simple setae scattered over lateral and distal margins. Left mandible (Fig. 4). Incisors with seven denticles (Fig. 4a). Prostheca robust, apically denticulate. Margin between prostheca and mola without setae; subtriangular process wide; setae absent from apex of mola. Right mandible (Fig. 5). Incisors with eleven denticles (Fig. 5a). Prostheca slender, apically denticulate; margin between prostheca and mola with two fine simple setae; setae present at apex of mola. Maxilla (Fig. 6). Inner dorsal row of setae with three denti-setae; two pectinate denti-setae and one apical dentiseta at same orientation of canines. Medial protuberance of galea with 2 long, fine and simple setae. Maxillary palp 1.1�� length of galea-lacinia. Palp segment II length subequal to length of segment I. Fine and simple setae scattered over segment II of maxillary palp. Labium (Fig. 7). Glossa shorter than paraglossa; inner margin with nine spine-like setae increasing in length distally; apex with two long, spine-like setae; outer margin with three long, spine-like setae; ventral surface with three short, fine, simple setae. Paraglossa sub-rectangular, curved inward; apex with two rows of long, fine, simple setae; ventral surface with row of four long, fine, spine-like setae; dorsal surface with two fine, spine-like setae near apex. Palp segment II with distomedial projection short and rounded; dorsally with row of three long, spine-like setae; ventrally with three simple spine-like setae. Palp segment III oblong, length 1.3�� width, with scattered simple setae and spine-like setae. Thorax ( Fig. 1). Light brown; metanotum with anterior region brown. Hind wing pad present. Gills absent from bases of coxae. Femur, tibia and tarsi light brown. Tibia I 0.9�� length of femur I; tibia II 0.8�� length of femur II; tibia III 0.8�� length of femur III. Tarsi I and II each 0.5�� length of respective femur; tarsi III 0.4�� length of femur III. Forefemur (Fig. 8) length about 3.4�� maximum width; outer margin with row of about 23 long, spine-like setae; inner margin with three short, fine, spine-like setae. Foretibia outer margin with few fine, simple setae; inner margin with three short, spine-like setae; anterior apex with two spine-like setae; patella-tibial suture present. Tarsal claws with 10 denticles each (Fig. 8a). Mid femur length about 2.6�� maximum width; outer margin with row of about 9 long, spine-like setae. Hind femur length about 3�� maximum width; outer margin with row of about 14 long, spinelike setae. Abdomen (Fig. 1). Coloration light brown, but segment VIII lighter. Segment X with cross-like color pattern. Posterior margin of tergum IV with truncate spines (Fig. 9). Gills oval. Gills IV (Fig. 10) light brown; margins with narrow spines intercalating short, fine, simple setae; tracheae pigmented. Gill I 1.4�� length of segment II; gill IV 1.8�� length of segment V; gill VII 1.4�� length of segment VIII. Paraproct (Fig. 11) with two spines near inner margin; without shagreened area. Cerci light brown with brown band at mid length; outer and inner margins of terminal filament and inner margins of cerci with tufts of long, simple setae; outer margins of cerci with spines at apex of each segment. Etymology. The specific epithet is an arbitrary combination of letters referring to Meta, the Colombian state where this species was found. Distribution. Thusfar, the species has been found only in the R��o Orotoy, in the Orinoco River basin of Meta, Colombia (Fig. 12). Comments. The general shape of the labial palp of Camelobaetidius metae is similar to that found in C. carolinae Boldrini & Pes, 2014, C. cruzi Boldrini & Pes, 2014 and C. matilei Thomas & P��ru, 2003. Camelobaetidius metae, however, can be distinguished from C. carolinae by the size of the denticles on the tarsal claws, with the latter species having the first denticle distinctly longer than others, while the denticles in C. metae are all similar in length. Camelobaetidius metae is distinguished from C. cruzi by the shape of labial palp segment II, which is subquandrangular in C. cruzi versus oblong in C. metae. Camelobaetidius metae is distinguished from C. matilei by the hind wing, which is present in C. metae but absent in C. matilei. Furthermore, C. metae presents an outer margin of the forefemur with a row of 23 clavate setae and a posterior margin of tergum IV with truncate spines, while C. carolinae presents an outer margin of the forefemur with a row of 18 long, spine-like setae and a posterior margin of tergum IV with rounded spines; C. cruzi presents an outer margin of the forefemur with a row of nine long, spinelike setae and the posterior margin of tergum IV with rounded spines, and C. matilei presents an outer margin of the forefemur with a row of three long, spine-like setae and a posterior margin of tergum IV with truncate spines. Material examined. Holotype: Male mature nymph (mounted on slides, medium Euparal), Colombia, Meta, Castilla La Nueva, Vereda Cacayal, R��o Orotoy, 3��52���9.0��� N, 73��38���18.6��� W, 419m, 02/vi/2011, Osorio D, Caro C, cols (CEBUC). Paratypes: fourteen mature nymphs (four specimens mounted on slides, medium: Euparal), same data as holotype (seven specimens deposited in CEBUC and seven specimens deposited in MIUQ). Additional material: 35 nymphs, same data as holotype deposited in MIUQ. Habitat (Fig. 13). The nymphs were collected from the R��o Orotoy, which is in the Orinoco River basin. The stream is meandering and open, with clear water. It is 20 m wide, has an average depth of 0.68 m and discharge of 2.7 m 3 /s for the sampling season (high water). The stream bed is composed of boulders and sandy clay substrate. The riverbank presents secondary vegetation, typical of a foothill forest. The water temperature was 25��C; pH was 6.4 units; dissolved oxygen content was high (9.0 mg/l), conductivity was low (16 ��S/cm)., Published as part of Salinas-Jimenez, Luis Gonzalo, Boldrini, R., Osorio-Ramirez, Diana Paola, Caro, Clara Ines & Rojas-Pe��a, Jose Ismael, 2019, A new species of Camelobaetidius Demoulin, 1966 (Ephemeroptera: Baetidae), from the Colombian Orinoco River basin, pp. 367-374 in Zootaxa 4656 (2) on pages 368-373, DOI: 10.11646/zootaxa.4656.2.9, http://zenodo.org/record/3368740, {"references":["Boldrini, R & Pes, A. M. O. (2014) Five new species of Camelobaetidius Demoulin, 1966 (Ephemeroptera: Baetidae), and redescription of Camelobaetidius mexicanus (Traver & Edmunds, 1968). Zootaxa, 3796 (3), 545 - 567. https: // doi. org / 10.11646 / zootaxa. 3796.3. 8","Thomas, A., Peru, N. & Horeau, V. (2003) Les Ephemeres de la Guyane Francaise. 8. Description de Camelobaetidiusmatilei n. sp. et cle de determination de especesguyanensis de ce genre (Ephemeroptera: Baetidae). Ephemera, 3 (2), 123 - 133."]}
- Published
- 2019
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15. Myopathy and hypertrophic cardiomyopathy with selective lysis of thick filaments
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Bertini, E., Bosman, C., Salviati, G., Boldrini, R., Servidei, S., Ricci, E., Del Nonno, F., Gagliardi, M. G., and Bevilacqua, M.
- Published
- 1993
- Full Text
- View/download PDF
16. DETECTION AND CLINICAL SIGNIFICANCE OF DISSEMINATED TUMOUR CELLS AT DIAGNOSIS IN BONE MARROW OF CHILDREN WITH LOCALISED RHABDOMYOSARCOMA: O.089
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McDowell, H. P., Donfrancesco, A., Milano, G. M., Clerico, A., Mannarino, O., Altavista, P., Boldrini, R., Cozza, R., Inserra, A., and Dominici, C.
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- 2005
17. Long term follow up of children with myocarditis treated by immunosuppression and of children with dilated cardiomyopathy
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Gagliardi, M Giulia, Bevilacqua, M, Bassano, C, Leonardi, B, Boldrini, R, Camassei, F Diomedi, Fierabracci, A, Ugazio, A G, and Bottazzo, G F
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- 2004
18. In vivo bladder regeneration using small intestinal submucosa: an experimental study
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CAIONE, P., BOLDRINI, R., CAPOZZA, N., DOMINICIS, M. D.E., HEFFAWI, N., and PALOMBARO, G.
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- 2004
19. A single-institution Wilmsʼ tumor and localized neuroblastoma series
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Boglino, C, Inserra, A, Madafferi, S, Jenkner, A, Camassei, F Diomedi, Boldrini, R, and Donfrancesco, A
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- 2004
20. Neuromyopathy and restrictive cardiomyopathy with accumulation of intermediate filaments: a clinical, morphological and biochemical study
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Bertini, E., Bosman, C., Ricci, E., Servidei, S., Boldrini, R., Sabatelli, M., and Salviati, G.
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- 1991
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21. Long term follow up of children with myocarditis treated by immunosuppression and of children with dilated cardiomyopathy
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Giulia Gagliardi, M., Bevilacqua, M., Bassano, C., Leonardi, B., Boldrini, R., Diomedi Camassei, F., Fierabracci, A., Ugazio, A.G., and Bottazzo, G.F.
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Myocarditis -- Drug therapy ,Myocarditis -- Research ,Myocarditis -- Patient outcomes ,Mortality -- Italy ,Immunosuppression -- Drug therapy ,Immunosuppression -- Patient outcomes ,Immunosuppression -- Research ,Cardiomyopathy, Dilated -- Care and treatment ,Cardiomyopathy, Dilated -- Research ,Cardiomyopathy, Dilated -- Patient outcomes ,Children -- Health aspects ,Children -- Diseases ,Pediatric research ,Health - Published
- 2004
22. PEDIATRIC TESTICULAR GERM CELL TUMORS: the ASSOCIAZIONE ITALIANA ONCOLOGIA PEDIATRICA (AIEOP) study
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TERENZIANI,M, CONTE,M, BIASONI,D, BOLDRINI,R, COLLINI,P, PINTO,D, SPREAFICO,F, BISOGNO,G, DE PASQUALE,M, CECCHETTO,G, INSERRA,A, D'ANGELO,P, SIRACUSA, Fortunato, TERENZIANI,M, CONTE,M, BIASONI,D, BOLDRINI,R, COLLINI,P, PINTO,D, SPREAFICO,F, BISOGNO,G, DE PASQUALE,M, CECCHETTO,G, INSERRA,A, D'ANGELO,P, and SIRACUSA,F.
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Testicular Germ Cell Tumors in childood, chemotherapy, pediatric surgery - Abstract
We describe the results of a therapeutic approach with a pediatric BEP regimen (bleomycin, etoposide, cisplatin) from the Associazione Italiana Ematologia Oncologia Pediatrica (AIEOP) study on testicular germ cell tumors (TGCT).
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- 2017
23. Prevalence of liver complications in pediatric patients on home parenteral nutrition: indications for intestinal or combined liver-intestinal transplantation
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Diamanti, A, Gambarara, M, Knafelz, D, Marcellini, M, Boldrini, R, Ferretti, F, Papadatou, B, and Castro, M
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- 2003
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24. A new species of Camelobaetidius Demoulin, 1966 (Ephemeroptera: Baetidae), from the Colombian Orinoco River basin
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SALINAS-JIMENEZ, LUIS GONZALO, primary, BOLDRINI, R., additional, OSORIO-RAMIREZ, DIANA PAOLA, additional, CARO, CLARA INES, additional, and ROJAS-PEÑA, JOSE ISMAEL, additional
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- 2019
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25. Phenotypic Features of Epidermolysis Bullosa Simplex due to KLHL24 Mutations in 3 Italian Cases
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Hachem, M, primary, Barresi, S, additional, Diociaiuti, A, additional, Boldrini, R, additional, Condorelli, A, additional, Capoluongo, E, additional, Proto, V, additional, Scuvera, G, additional, Has, C, additional, Tartaglia, M, additional, and Castiglia, D, additional
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- 2019
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26. Next Generation Sequencing Uncovers a Rare Case of X-linked Ichthyosis in an Adopted Girl Homozygous for a Novel Nonsense Mutation in the STS Gene
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Diociaiuti, A, primary, Angioni, A, additional, Pisaneschi, E, additional, Margollicci, M, additional, Boldrini, R, additional, Alesi, V, additional, Novelli, A, additional, Zambruno, G, additional, and Hachem, M, additional
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- 2019
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27. Ulmeritoides passorum Gama-Neto & Hamada 2014
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Boldrini, R. and Lima, L. R. C.
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Ulmeritoides ,Insecta ,Arthropoda ,Ulmeritoides passorum ,Leptophlebiidae ,Animalia ,Biodiversity ,Ephemeroptera ,Taxonomy - Abstract
Ulmeritoides passorum Gama-Neto & Hamada, 2014 (Fig. 14���24) Diagnoses. Female imago:1) large body size (> 7.7 mm); 2) base of wings tinged with brown (Fig. 14). Nymph: 1) medial denticle of anteromedial emargination of labrum much larger than others; 2) tusk on inner margin of maxillae hardly developed; 3) posterolateral projections present on abdominal segments II���IX; 4) lines of pectinate setae on ventral surface of tibia III forming one main line. Female imago. Length: body: 7.7���8.8 mm; forewings: 9.3���10.1mm; hind wings: 1.4���1.5 mm. General color light brown (Figs. 14), darker in the living specimen (Fig. 24). Head (Fig. 14): Washed with black. Scape and pedicel brown; ocelli white, blackish basally. Thorax (Fig. 14): Nota with anteronotal protuberance, medioscutum, submedioscutum, posterior scutal protuberance and scutellum brown; sterna brown.. Wings (Figs. 15, 16, 16 a): membrane of forewing hyaline, wing base brownish; main longitudinal and intercalaries veins dark brown, cross-veins pale yellow; one cross-vein basal to bulla. Membrane of hind wings (Figs. 16, 16 a) hyaline; longitudinal veins brownish, cross-veins pale yellow. Legs: Fore femur brown, fore tibia and fore tarsus dark brown Abdomen (Fig. 14): when without eggs, light brown. Nymph. Length: Body: 6.9���7.3 mm. Nymphal exuviae: Tibia I: 1.2 mm; Tibia II: 1.2 mm; Tibia III: 1.4 mm. General coloration (Fig. 17): Brown. Labrum (Fig. 18): Subrectangular, broader than long; with one middle large and apically rounded denticle. Mandibles: Apical 1/2 of right mandible with inner and outer margin with fine long setae; inner and outer incisive, respectively, with two and three denticles. Left mandible (Fig. 19) with outer margin with fine long setae; inner and outer incisive with three denticles. Maxilla (Fig. 20, 20 a): Tusk on inner apical margin hardly developed; one robust and fifteen subapical pectinate setae. Labium: Segment I with fine, simple setae scattered on inner margin; and outer margin with a row of setae increasing in length toward apex, and pectinate, long setae near apex. Segment II with fine, simple setae scattered on inner and outer margin; dorsally with a diagonal row of nine simple setae. Segment III with fine, simple setae scattered on inner and outer margin. Thorax: Light brown. Legs: Light brown. Foretibia and foretarsi brown. Leg I (Fig. 21): Femur outer margin with long, pectinate setae; dorsal surface with long, pectinate setae near outer margin, and with spine-like setae and short, fine, pectinate setae near inner margin, and with spine like setae on midline. Tibia inner margin with a row of long, pectinate setae; dorsal and ventral surface with fine, long, simple setae near outer margin, and with short, fine pectinate setae near inner margin. Tarsi inner margin with a row of short, pectinate setae, and outer margin with fine, long simple setae. Claws with eight denticles increasing in length toward apex. Leg II (Fig. 22): Femur inner margin with a row of spine-like setae; outer margin with long pectinate setae and a row of spine-like setae; dorsal surface with spine-like setae on margins and at the midline. Tibia inner margin with a row of long, pectinate setae; dorsal and ventral surface with fine, long, simple setae near outer margin; dorsal surface with six spine-like setae near inner margin. Leg III (Fig. 23): Femur outer and inner margin with a row of spine-like setae; dorsal surface with spine-like setae on margins and at the midline. Tibia inner and outer margins with a row spine-like setae; dorsal surface with a row of spine-like setae near inner margin; ventral surface with one row of pectinate setae. Abdomen: Terga brown. Posterolateral projections lighter, present on abdominal segments II-IX. Sterna brown. Gills gray, tracheae and fimbriae gray-violet. Caudal filaments light brown. Distribution. Brazil: Roraima: Amajari and Pacaraima. Material examined. Four female imago, Brazil, Roraima, Amajari, Serra do Tepequ��m, Igarap�� Cocal, 3��73���N/ 61��72���W, 01.xi.2014, Boldrini, R. coll (UFRR). Two nymphs (one mature nymph), Brazil, Roraima, Amajari, Serra do Tepequ��m, Igarap�� Cocal, 3��73���N/ 61��72���W, 01.xi.2014, Boldrini, R. coll (UFRR). One immature nymph, Brazil, Roraima, Amajari, Serra do Tepequ��m, Igarap�� Cocal, 3��73���N/ 61��72���W, 18.xii.2014, Boldrini, R., Barroso, P.C.S. colls (UFRR). One male imago, Brazil, Roraima, Amajari, Serra do Tepequ��m, Igarap�� Cocal, 3��73���N/ 61��72���W, 18.xii.2014, Boldrini, R coll (UFRR). Three male imago, Brazil, Roraima, Pacaraima, Igarap�� Sam��, 4��28���30.8������N / 61��09���44.4������W, 12.vi.2014, Boldrini, R. coll (UFRR). Three male imago, Brazil, Roraima, Pacaraima, Igarap�� Sam��, 4��28���30.8������N / 61��09���44.4������W, 11.vi.2014, Boldrini, R. coll (UFRR). One male imago (reared), Brazil, Roraima, Amajari, Serra do Tepequ��m, Igarap�� Cocal, 3��73���N/ 61��72���W, 18.xii.2014, Boldrini, R. coll (UFRR). Comments. The adults of U. passorum have similar characters to U. flavopedes adults. The males share the apex of the penis lobe rounded, and males and females share the forewing with costal and subcostal areas hyaline. Both species can be found in savanna areas in the Roraima State, but the U. passorum specimens can be found at altitudes>300 meters above the sea level, while U. flavopedes can be found in areas near 100 meters above the sea. The adults of U. passorum are easily recognized by the presence of brownish wing bases., Published as part of Boldrini, R. & Lima, L. R. C., 2017, New species and stages description of Ulmeritoides Traver, 1959 (Ephemeroptera: Leptophlebiidae) from Roraima State, Northern Brazil, pp. 385-394 in Zootaxa 4282 (2) on pages 390-393, DOI: 10.11646/zootaxa.4282.2.11, http://zenodo.org/record/818787, {"references":["Gama Neto, J. L. & Hamada, N. (2014) Leptophlebiidae (Ephemeroptera) of the Serra do Tepequem, Roraima State, Brazil: new records and description of two new species. Zootaxa, 3900 (2), 279 - 286."]}
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- 2017
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28. New species and stages description of Ulmeritoides Traver, 1959 (Ephemeroptera: Leptophlebiidae) from Roraima State, Northern Brazil
- Author
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Boldrini, R. and Lima, L. R. C.
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Insecta ,Arthropoda ,Leptophlebiidae ,Animalia ,Biodiversity ,Ephemeroptera ,Taxonomy - Abstract
Boldrini, R., Lima, L. R. C. (2017): New species and stages description of Ulmeritoides Traver, 1959 (Ephemeroptera: Leptophlebiidae) from Roraima State, Northern Brazil. Zootaxa 4282 (2): 385-394, DOI: https://doi.org/10.11646/zootaxa.4282.2.11
- Published
- 2017
29. Ulmeritoides amajari Boldrini & Lima 2017, sp. nov
- Author
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Boldrini, R. and Lima, L. R. C.
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Ulmeritoides ,Insecta ,Arthropoda ,Ulmeritoides amajari ,Leptophlebiidae ,Animalia ,Biodiversity ,Ephemeroptera ,Taxonomy - Abstract
Ulmeritoides amajari sp. nov. (Figs. 1���13) Diagnoses. Ulmeritoides amajari sp. nov. can be separated from the other species of the genus by the following combination of characters. In the imago: 1) forewings hyaline, base brown and without dark spots in bullae in Sc and R1 (Fig. 4); 2) one cross-veins basal to bulla in forewings (Fig. 4); 3) abdominal color pattern as in Fig. 1; 4) penis lobe ending in an rounded apical projection (Fig. 7). In the female: forewings hyaline, base brown and without dark spots in bullae in Sc and R1; 2) one cross-veins basal to bulla in forewings; 3) abdominal color pattern as in Fig. 2. In the nymph: 1) medial denticle on anteromedian emargination of labrum much larger than others (Figs. 8, 8 a); 2) tusk on inner margin of maxillae well developed (Figs. 10, 10 a); 3) dorsum of fore femur with numerous, acute spines (Fig. 11); 4) lines of pectinate setae on ventral surface of tibia III forming one main line (Fig. 13). Male Imago. Length: body: 5.5���6.2 mm; forewings: 5.7���6.5 mm; hind wings: 1.0��� 1.1 mm. General coloration brown (Fig. 1). Head: flagellum light brown; ocelli white, reddish brown basally; upper portion of eyes reddish, lower portion blackish. Thorax: pro-, meso- and metanotum brown; pro-, meso-, and metasternum reddish brown. Wings (Figs. 4, 5, 5 a): membrane of forewings (Fig. 4) hyaline, wing base light brown; main longitudinal and intercalaries veins light brown, cross-veins hyaline; one cross-vein basal to bulla. Membrane of hind wings (Figs. 5, 5 a) hyaline; longitudinal and cross-veins hyaline. Legs (Fig. 1): Yellowish; middle region of all femora with short blackish mark. Leg I: femur washed with reddish brown; tibia washed with light brown, subapical portion of tibia with a black band; tarsi II and III washed with light brown. Tibia II and III with a reddish brown band apically. Abdomen (Fig. 1): Terga reddish brown. Terga I, II and III darker. Terga I���VIII with lateral light reddish brown spots. Sterna light reddish brown. Genitalia (Fig. 6): penis yellowish, washed with reddish brown basally, apex yellowish. Forceps yellowish, segment I and II washed with light brown. Apex of penis lobe ending in a inner rounded apical projection (Fig. 7), and with a lateral pointed projection (7a). [Caudal filaments broken off and lost]. Female imago. Length: body: 5.7���6.3 mm; forewings: 5.1���7.1 mm; hind wings: 1.1 mm. General color light brown (Fig. 2). Head: pedicel brown; ocelli white, blackish basally; area between lateral ocelli and eyes brown. Thorax: Lateral margins of pronotum and pleura blackish. Wings. Wings membrane hyaline. Similar to male imago. Legs (Fig. 2): light brown; middle region of all femora with short blackish marks; Leg I: femur washed with brown; tibia washed with light brown, subapical portion of tibia with a black band. Subbasal region of hind femur with a longitudinal blackish stripe. Tarsi II and III washed with black. Abdomen (Fig. 2): Terga I, II and III darker; terga I���VIII with lateral light brown spots, and terga IV���VIII with a medial light brown spots. Sterna I���III darker. [Caudal filaments broken off and lost]. Nymph. Length: body: 6.3���7.1 mm. Nymphal exuviae: Tibia I: 1.2 mm; Tibia II: 1.1 mm; Tibia III: 1.3 mm. General coloration: light brown to brown. Labrum (Figs. 8, 8 a): Subrectangular, broader than long; with one middle larger and apically rounded denticle and four inconspicuous denticles. Mandibles: Apical 1/2 of right mandible with inner and outer margin with fine long setae; inner and outer incisors with two and three denticles respectively. Apical 1/2 of left mandible (Fig. 9) with outer margin with fine long setae; inner and outer incisors with three denticles. Maxilla (Figs. 10, 10 a): Small tusk on inner apical margin present; one robust and twelve subapical pectinate setae. Labium: Segment I with fine, simple setae scattered on inner margin; outer margin with a row of setae increasing in length toward apex, long pectinate setae near apex. Segment II with fine, simple setae scattered on inner and outer margin; dorsally with a diagonal row of seven simple setae. Segment III with fine, simple setae scattered on inner and outer margin. Thorax (Fig. 3): nota light brown. Leg I (Fig. 11): Brown; subapical region of tibia dark brown. Femur outer margin with long, pectinate setae; dorsal surface with long, pectinate setae near outer margin, inner margin with spine-like pectinate setae and with spine-like setae on midline. Tibia inner margin with a row of long, pectinate setae; dorsal and ventral surface with fine, long, simple setae near outer margin, and with spine-like setae near inner margin. Tarsi inner margin with a row of spine-like setae, and outer margin with fine, long simple setae. Claws with six denticles increasing in length toward apex. Leg II (Fig. 12): Femur similar to leg I. Leg III (Fig. 13): Subapical region of tibia and tarsi dark brown. Femur outer margin with simple setae; inner margin with a row of spine-like setae. Tibia ventral surface with a row of long, pectinate setae; dorsal surface with a row of spine-like setae near inner margin; apex with five long, pectinate setae. Abdomen (Fig 3): terga brown. Posterolateral projections lighter, present on abdominal segments II-IX. Sterna light brown. Gills gray. [Caudal filaments broken off and lost]. Distribution. Brazil: Roraima: Amajari municipality. Material examined. Holotype. Male imago (reared), Brazil, Roraima, Amajari, Rio Amajari, below the bridge, 3��39���51.6������N / 61��21���32.46������W, 30.iii.2016, Boldrini, R. coll (INPA). Paratypes: Three female imago, Brazil, Roraima, Amajari, Rio Amajari, below the bridge, 3��39���51.6������N / 61��21���32.46������W, 16.ii.2016, Boldrini, R., Lima, L.R.C. coll (UFRR). Two female and one male imagos (all reared), Brazil, Roraima, Amajari, Rio Amajari, below the bridge, 3��39���51.6������N / 61��21���32.46������W, 16.ii.2016, Boldrini, R., Lima, L.R.C. colls (CZNC). Two female and three male imagos, Brazil, Roraima, Amajari, Rio Amajari, below the bridge, 3��39���51.6������N / 61��21���32.46������W, 30.iii.2016, Boldrini, R. coll (UFRR). Three imature nymphs, Brazil, Roraima, Amajari, Rio Amajari, below the bridge, 3��39���51.6������N / 61��21���32.46������W, 30.iii.2016, Boldrini, R. coll (UFRR). Etymology. Amajari, name of the river where the material was collected. Comments. The male imago of Ulmeritoides amajari sp. nov. have a forewing coloration and penis shape similar to that of the widespread species U. uruguayensis. Ulmeritoides amajari sp. nov. is distinguished from U. uruguayensis by apex of penis lobe; U. amajari sp. nov. has the apex of penis lobe ending in a inner rounded apical projection, while U. uruguayensis has the apex of penis lobe ending in a inner pointed apical projection. The nymphs of both species can be distinguished by lines of pectinate setae on ventral surface of tibia III (U. amajari has one main line, while U. uruguayensis, has two lines)., Published as part of Boldrini, R. & Lima, L. R. C., 2017, New species and stages description of Ulmeritoides Traver, 1959 (Ephemeroptera: Leptophlebiidae) from Roraima State, Northern Brazil, pp. 385-394 in Zootaxa 4282 (2) on pages 386-390, DOI: 10.11646/zootaxa.4282.2.11, http://zenodo.org/record/818787
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- 2017
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30. Nd:YAG laser tissue welding in urinary reconstruction: an experimental study: O
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Capozza, N., Creti, G., Boldrini, R., Matarazzo, E., Lais, A., and Caione, P.
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- 1998
31. Lymphangioma of the scrotum in a pediatric patient: Ultrasound and magnetic-resonance findings
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Orazi, C., Fariello, G., Malena, S., Ferro, F., and Boldrini, R.
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- 1994
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32. “Brown tumor” of the maxilla
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Fasanelli, S., Graziani, M., Boldrini, R., and Bosman, C.
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- 1992
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33. Miroculis (Miroculis) cohnhafti Boldrini 2017, sp. nov
- Author
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Boldrini, R.
- Subjects
Insecta ,Arthropoda ,Leptophlebiidae ,Animalia ,Biodiversity ,Miroculis ,Ephemeroptera ,Miroculis cohnhafti ,Taxonomy - Abstract
Miroculis (Miroculis) cohnhafti sp. nov. Raimundi & Nascimento (Figs. 16���21) Diagnosis. Miroculis (Miroculis) cohnhafti sp. nov. differs from the other species of Miroculis by the following characteristics in the male imago: 1) compound eye with upper portion on a stalk of intermediate length (approximately as long as wide); 2) distomedial projection of compound eyes present (little developed in some specimens) (Fig. 16); 3) forewing with apical membrane between C and R pale (Fig. 18); 4) veins A1 and A2 free (Fig. 18); 5) forceps segment II with inner margin of apical portion broadened (Fig. 20). Description. Measurements (mm). Male imago. Body: 5.8; forewing: 4.6; angularity of cubital region 100��; hind wing: 1.2; fore leg: 4.0. Caudal filament: broken off and missing. Ratios. Wings: Forewing width 0.4 x forewing length; hind wing length 0.3 forewing wing length; hind wing width 0.46 hind wing length; fork on MA of forewing 0.5 x total length of MA. Legs: fore leg segments: 1.1: 1.00: (1.70 mm) 0.08: 0.62: 0.47: 0.26: 0.14. Genitalia: medial length of styliger plate 0.4 x maximum width of styliger plate; lateral length of styliger plate 1.0 x medial length of styliger plate; lateral length of styliger plate 0.4 x lateral length of segment IX. Forceps segment III 0.8 x segment II; segment III 0.3 x segment I; segment II 0.4 x segment I; penis length 0.9 x forceps segment I. Coloration (Figs. 17���20). Body: brownish. Head. Orangish brown, with dark brown marks ventrally. Compound eye with stalk orange, lighter toward apex; facets of upper portion orange, separated by dark brown grooves. Antenna: scape yellowish, pedicel brown and flagellum whitish translucent. Thorax: brown, sutures darker; membranous areas yellowish. Wings (Figs. 18���19): forewings with hyaline membrane; longitudinal veins brown, with C, SC and R darker; cross-veins brown, weakly developed. Hind wing with membrane hyaline; veins brown. Legs: Fore leg brownish; femur with medial blackish mark and subapical blackish band; tibia with a subapical blackish band. Mid and hind legs: coxae brown, remainder whitish brown; femora with a medial gray mark and subapical blackish band; tibiae with a subapical blackish mark. Abdomen: segments I ��� VI translucent brown, remainder orangish brown; terga with dark brown marks on medial and sublateral regions. Genitalia (Figs. 20���21). Styliger plate brown, washed with orange���yellow. Forceps brown slightly washed with orange, except basal 2/3 of segment I and apical region of segment III light brown. Penis brown washed with yellow. Caudal filament: whitish brown, darker basally on each segment. Morphology: Head: posterior margin V-shaped. Compound eye with upper portion on a stalk of intermediary length (approximately as long as wide); distomedial projection of compound eyes present; dorsal surface rounded, with 11 facets on the longest row. Lower portion of compound eye elliptic. Wings: forewing with three cross veins between C and Sc basal to bulla; 11 cross veins between C and Sc; IMP connected to MP1 and MP2 by a cross vein; MP2 connected to MP1 and CuA by cross veins; CuA connected to CuP by cross vein subbasally; veins A1 and A2 free. Hind wing with apex rounded, fork of R+MA symmetric, CuP present. Legs: fore tarsal claw different, one spine-like and other rounded. Genitalia: Styliger plate with posteromedial margin concave. Forceps segment I narrowing gradually toward apex without broad basal portion; inner and outer margins undulated; forceps segment II with inner margin broadened apically. Penis broad basally, narrowing toward apex; apex bifurcated (Fig. 21): inner bifurcation short and acute; outer bifurcation longer and rounded; without row of spines on inner margin. Etymology. This species is named in honor of Mario Cohn Haft, one of the organizers of the expedition to the Serra da Mocidade. Distribution: Brazil: Roraima State. Material examined: Holotype: male imago, BRAZIL, Roraima State, Caracara�� municipality, Serra da Mocidade National Park, 01��42���21.3���N / 061��47���04.7���W, 03���04.ii. 2016, Nascimento, J.M.C. col. (INPA). Paratypes: ten male imagos (same data as holotype) (five at INPA; five at CZNC). Five male imago, BRAZIL, Roraima State, Caracara�� Caracara�� municipality, Serra da Mocidade National Park, 01��36.240"N / 061��54.370'W, 15���25.i. 2016, Boldrini, R., Barroso, P.C.S. and Xavier, F.F. col. (UFRR). Comments: Miroculis (Miroculis) cohnhafti sp. nov. shares some characteristics with Miroculis (M.) stenopterus Lima, Raimundi, Pinheiro & Salles, 2014, Miroculis (M.) marauiae Savage & Peters, 1983 and Miroculis (M.) eldorado Gama-Neto & Hamada, 2014. These four species have the upper portion of the compound eyes on a stalk, forewings are hyaline without any pigmentation, distomedial portion of styliger plate is concave, or concave to slightly straight in M. (M.) marauiae, and hind wings are hyaline, but washed with brown on the base in M. (M.) stenopterus, absent in M. (M.) marauiae and in subcostal area in M. (M.) eldorado. Otherwise, Miroculis (M.) cohnhafti sp. nov. differs from these mentioned species above by distomedial projection of compound eyes present, while it is absent in the others species., Published as part of Boldrini, R., 2017, Three new species of Miroculis from the Serra da Mocidade National Park, Roraima State, Brazil, with new records and checklist of the Leptophlebiidae (Ephemeroptera), pp. 573-583 in Zootaxa 4317 (3) on pages 579-581, DOI: 10.11646/zootaxa.4317.3.8, http://zenodo.org/record/884837, {"references":["Lima, L. R. C., Raimundi, E. A., Pinheiro, U. & SalleS, F. F. (2014) A new SpecieS of Miroculis EdmundS, 1963 (Ephemeroptera: Leptophlebiidae) from NortheaStern Brazil. Zootaxa, 3795 (4), 441 - 448.","Gama-Neto, J. L. & Hamada, N. (2014) Leptophlebiidae (Ephemeroptera) of the Serra do Tepequem, Roraima State, Brazil: New recordS and deScription of two new SpecieS. Zootaxa, 3900 (2), 279 - 286."]}
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- 2017
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34. Simothraulopsis demerara
- Author
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Boldrini, R.
- Subjects
Insecta ,Simothraulopsis ,Arthropoda ,Leptophlebiidae ,Animalia ,Simothraulopsis demerara ,Biodiversity ,Ephemeroptera ,Taxonomy - Abstract
Simothraulopsis demerara (Traver, 1947) Distribution: Venezuela, Colombia, French Guyana and Suriname. Brazil: States of Roraima, Pará, Amazonas, Bahia, Espírito Santo, Pernambuco. Material examined: Two male imago, BRAZIL, Roraima State, Caracaraí municipality, Serra da Mocidade National Park, 01°36.240"N / 061°54.370'W, 15–25.i. 2016, Boldrini, R., Barroso, P.C.S. and Xavier, F.F. col. (UFRR). Ten male subimago, BRAZIL, Roraima State, Caracaraí municipality, Serra da Mocidade, 01°42’21.3”N / 061°47’04.7”W, 03–04.ii. 2016, Nascimento, J.M.C. col. Pennsylvania trap., Published as part of Boldrini, R., 2017, Three new species of Miroculis from the Serra da Mocidade National Park, Roraima State, Brazil, with new records and checklist of the Leptophlebiidae (Ephemeroptera), pp. 573-583 in Zootaxa 4317 (3) on page 581, DOI: 10.11646/zootaxa.4317.3.8, http://zenodo.org/record/884837
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- 2017
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35. Paramaka convexa
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Boldrini, R.
- Subjects
Insecta ,Arthropoda ,Leptophlebiidae ,Animalia ,Biodiversity ,Paramaka convexa ,Paramaka ,Ephemeroptera ,Taxonomy - Abstract
Paramaka convexa (Spieth, 1943) (Fig. 23) Distribution: Surinam, French Guyana, Venezuela and Colombia. Brazil: States of Roraima (new record), Pará, Mato Grosso and Bahia. Material examined: One male adult, BRAZIL, Roraima State, Caracaraí municipality, Serra da Mocidade National Park, waterfall of the Pacu river, 01°36.060'N / 061°54548'W, 15–25.i. 2016, Boldrini, R., Barroso, P.C.S. and Xavier, F.F. col. (UFRR)., Published as part of Boldrini, R., 2017, Three new species of Miroculis from the Serra da Mocidade National Park, Roraima State, Brazil, with new records and checklist of the Leptophlebiidae (Ephemeroptera), pp. 573-583 in Zootaxa 4317 (3) on page 581, DOI: 10.11646/zootaxa.4317.3.8, http://zenodo.org/record/884837
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- 2017
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36. Miroculis (Miroculis) xavieri Boldrini 2017, sp. nov
- Author
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Boldrini, R.
- Subjects
Insecta ,Arthropoda ,Miroculis xavieri ,Leptophlebiidae ,Animalia ,Biodiversity ,Miroculis ,Ephemeroptera ,Taxonomy - Abstract
Miroculis (Miroculis) xavieri sp. nov. Raimundi & Boldrini (Figs. 11���15) Diagnosis. Miroculis (Miroculis) xavieri sp. nov. differs from the other species of Miroculis by the following characteristics in the male imago: 1) distomedial projection of compound eyes absent; 2) forewing with brown areas along cross veins at base from Sc to MA and at MP; hind wing brown at apex and on cross veins basally (Figs. 12���13); 3) forceps washed with dark grey on apical third of segment I, and on segment II; 4) forceps segment I with basal half broad and tapering gradually toward apex; 5) penis broad basally, narrowing gradually toward apex. Description. Measurements (mm). Male imago. Body: 5.0; forewing: 5.0; angularity of cubital region 100��; hind wing: 1.5; legs: broken off and missing except for the right foreleg. Caudal filament: 11.0. Ratios. Wings: Forewing width 0.4 x forewing length; hind wing length 0.3 forewing length; hind wing width 0.5 x hind wing length; fork on MA of forewing 0.5 x total length of MA. Genitalia: medial length of styliger plate 0.5 x maximum width of styliger plate; lateral length of styliger plate 1.2 x medial length of styliger plate; lateral length of styliger plate 1.2 x lateral length of segment IX. Forceps segment III 0.9 x segment II; segment III 0.4 x segment I; segment II 0.4 x segment I; penis length 1.0 x forceps segment I. Coloration (Figs. 11���14). Body: brownish. Head. Orange, ventrally washed with dark brown. Compound eye with stalk orange, lighter toward apex; facets of upper portion orange, separated by dark brown grooves. Antenna pale washed with brown. Thorax: brown, darker on margins. Wings (Figs. 12���13): forewings with hyaline membrane and brown clouds along cross veins at base from Sc to MA and at MP. Hind wing with hyaline membrane; brown on apex and on cross veins basally. Legs: fore leg pale with dark brown marks on the middle and apex of femur. Abdomen: terga and sterna light brown; terga I���IX with a transversal dark brown band on posterolateral portion, and brown mark on posteromedian portion of terga VII���VIII. Genitalia (Fig. 14): brown. Styliger plate washed with dark brown. Forceps washed with dark grey on apical third of segments I and II. Penis brown. Caudal filament: brown, darker basally on each segment. Morphology: Head: posterior margin V-shaped. Compound eye with upper portion on a mid-stalk (as long as wide); distomedial projection of compound eyes absent; dorsal surface rounded, with ten facets in the longest row. Lower portion of compound eye elliptic. Wings: forewing with four to five cross veins between C and Sc basal to bulla; 11���14 cross veins between C and Sc; IMP free basally; MP2 connected to base of MP1 by cross vein; CuA connected to CuP by cross vein subbasally; veins A1 and A2 connected by a cross vein subbasally. Hind wing with apex slightly acute; fork of R+MA symmetrical; CuP present. Genitalia: Styliger plate with posteromedial margin convex. Forceps segment I with basal portion broad narrowing gradually toward apex, apical third of inner margin broad; inner margin undulated. Penis broad basally, narrowing gradually toward apex (Fig. 14); apex rounded (Fig. 15); a row of spines on base of inner margin absent. Etymology: We are honoured in naming this species to our friend Francisco F. Xavier Filho, Instituto Nacional de Pesquisas da Amaz��nia, in recognition of his extensive support in field trips to collect insects in the Amazonian region of Brazil. Distribution: Brazil: Roraima State. Material examined: Holotype: male imago, BRAZIL, Roraima State, Caracara�� municipality, Serra da Mocidade National Park, 01��42"N / 61��27'W, 25.i���06.ii 2016, Mahlmann, T. col. (CZNC). Paratype: three male imago, same data as holotype (CZNC). Four male imago, BRAZIL, Roraima State, Caracara�� municipality, Serra da Mocidade National Park, 01��42"N / 061��47'W, 25.i���06.ii. 2016, Oliveira, M., Mahlmann, T. and Xavier, F.F. col. (UFRR). Comments: Miroculis (Miroculis) xavieri sp. nov. shares some characteristics with Miroculis (Miroculis) nebulosus Savage, 1987 such as, compound eye with a short stalk (length 0.5 x width), forewing with MP2 connected to MP1 by a cross vein and IMP free basally; and hind wing brown apically (Fig. 13). However, M. (M.) xavieri sp. nov. differs from M. (M.) nebulosus by having a hyaline forewing with crossveins tinged brown, base of forceps segment I with basal third portion narrowing gradually toward apex and washed with dark gray on apical third, and penis as long as forceps segment I. Otherwise, M. (M.) nebulosus has the forewing completely washed with brown and with few hyaline areas, forceps segment I with basal third narrowing abruptly toward apex and equally pigmented as in the other segments, and penis longer than segment forceps I (1.5 x)., Published as part of Boldrini, R., 2017, Three new species of Miroculis from the Serra da Mocidade National Park, Roraima State, Brazil, with new records and checklist of the Leptophlebiidae (Ephemeroptera), pp. 573-583 in Zootaxa 4317 (3) on pages 577-579, DOI: 10.11646/zootaxa.4317.3.8, http://zenodo.org/record/884837, {"references":["Savage, H. M. (1987) Two new SpecieS of Miroculis from Cerro de la Neblina, Venezuela with new diStribution recordS for Miroculis fittkaui and Microphlebia surinamensis (Ephemeroptera: Leptophlebiidae). Aquatic Insects, 9, 97 - 108. httpS: // doi. org / 10.1080 / 01650428709361279"]}
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- 2017
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37. Miroculis (Miroculis) mocidade Boldrini 2017, sp. nov
- Author
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Boldrini, R.
- Subjects
Insecta ,Arthropoda ,Leptophlebiidae ,Animalia ,Biodiversity ,Miroculis ,Ephemeroptera ,Taxonomy ,Miroculis mocidade - Abstract
Miroculis (Miroculis) mocidade sp. nov. Raimundi, Boldrini & Nascimento (Figs. 6���10) Diagnosis. Miroculis (Miroculis) mocidade sp. nov. differs from the other species of Miroculis by the following characteristics in the male imago: 1) distomedial projection of compound eyes absent; 2) forewing with distal region washed with brown, hind wing with a tiny brownish spot on the base (Fig. 8); 3) forewing with vein A1 connected to A2; 4) forceps washed with brown; 5) forceps segment I with inner basal third narrowing abruptly, and inner subapical portion well broadened (Fig. 9); 6) penis broad basally, narrowing gradually toward apex; apex rounded (Fig. 10). Description. Measurements (mm). Male imago. Body: 6.0; forewing: 5.0, angularity of cubital region 98��; hind wing: 1.2. Fore leg: broken off and missing, except for coxa, trochanter, femora and tibiae; mid leg: 2.7; hind leg: broken off and missing. Caudal filament: 11.7. Ratios. Wings: Forewing width 0.5 x forewing length; hind wing length 0.3 x forewing length; hind wing width 0.5 hind wing length; fork on MA of forewing 0.5 x total length of MA. Genitalia: medial length of styliger plate 0.4 x maximum width of styliger plate; lateral length of styliger plate 1.2 x medial length of styliger plate; lateral length of styliger plate 0.7 x lateral length of segment IX. Forceps segment III 0.9 x segment II; segment III 0.4 x segment I; segment II 0.5 x segment I; penis length 1.2 x forceps segment I. Coloration (Figs. 6���9). Body: brownish. Head. Brown. Compound eye with stalk orange, lighter toward apex; facets of upper portion orange, separated by brown grooves. Antenna: scape and pedicel brown, flagellum light brown. Thorax: brown, darker on margins. Wings (Figs. 7���8): forewings with hyaline membrane, distal region washed with brown, cross veins surrounded by small brown areas. Hind wing with hyaline membrane with a tiny brownish cloud on the base. Legs: pale; fore leg with femur and tibia washed with light brown, apex of femur with a brown band; mid leg with a brown band on apex; hind leg with a brown stripe on mid region and a brown band on apex. Abdomen: whitish washed with light brown, segments VIII���X washed with brown. Genitalia (Fig. 9): whitish washed with brown. Penis washed with dark brown. Caudal filament: whitish washed with light brown, lighter toward apex. Morphology: Head: posterior margin V-shaped. Compound eye with upper portion on a short stalk (0.5 x longer than wide); distomedial projection of compound eyes absent; dorsal surface elliptic, with ten facets in the longest row. Lower portion of compound eye elliptic. Wings: forewing with five cross veins between C and Sc basal to bulla; 14���17 cross veins between C and Sc; IMP free basally; MP2 not or weakly connected to base of MP1 by cross vein; CuA connected to CuP by a cross vein subbasally; CuP and A1 connected by a cross vein subbasally; A1 connected to A2. Hind wing with apex slightly acute; fork of R+MA symmetrical; CuP present. Genitalia: Styliger plate with posteromedial margin concave. Forceps segment I with basal third broad tapering abruptly toward apex, and inner margin with subdistomedial portion broadened; inner and outer margins undulated. Penis broad narrowing gradually toward apex; apex rounded (Fig. 10); a row of spines on base of inner margin absent. Etymology: The specific epithet of the new species derives from the locality where the imagos were collected: Serra da Mocidade National Park. Distribution: Brazil: Roraima State. Material examined: Holotype: male imago, BRAZIL, Roraima State, Caracara�� municipality, Serra da Mocidade National Park, 01��42"N / 61��27'W, 25.i���06.ii.2016, Mahlmann, T. col. (CZNC). Paratypes: one male imago, BRAZIL, Roraima State, Caracara�� municipality, Serra da Mocidade National Park, 01��42"N / 061��47'W, 25.i���06.ii.2016, Oliveira, M., Mahlmann, T. and Xavier, F.F. col. (UFRR); two male imagos, same data as holotype, except by 01��42���17.3���N / 061��47���14.1���W, 01���02.ii.2016, Nascimento, J.M.C. (INPA). Comments. Miroculis (Miroculis) mocidade sp. nov. and Miroculis (Miroculis) fittkaui Savage & Peters, 1983 share a hyaline forewing which is pigmented in some parts of membrane especially around cross veins; and forceps segment I with subdistomedial broadened (Fig. 9). However, M. (M.) mocidade sp. nov. differs from M. (M.) fittkaui by displaying the hind wing with a tiny dark mark only in the base (Fig. 8) while in M. (M.) fittkaui the dark mark is in the base and the apex; by the forceps pigmented and pale in the inner margin of apical 2/3 of forceps segment I (Fig. 9), while M. (M.) fittkaui has only apex of forceps segment I; and by the shape of forceps segment I, M. (M.) mocidade has a short subdistomedial projection, with 1/ 4x length of forceps segment I, while M. (M.) fittkaui has a large subdistomedial projection, with 2/ 3x length of forceps segment I., Published as part of Boldrini, R., 2017, Three new species of Miroculis from the Serra da Mocidade National Park, Roraima State, Brazil, with new records and checklist of the Leptophlebiidae (Ephemeroptera), pp. 573-583 in Zootaxa 4317 (3) on pages 575-577, DOI: 10.11646/zootaxa.4317.3.8, http://zenodo.org/record/884837
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- 2017
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38. Hermanellopsis arsia Savage and Peters 1983
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Boldrini, R.
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Insecta ,Arthropoda ,Leptophlebiidae ,Animalia ,Biodiversity ,Hermanellopsis ,Ephemeroptera ,Hermanellopsis arsia ,Taxonomy - Abstract
Hermanellopsis arsia Savage and Peters, 1983 (Fig. 22) Distribution: Brazil: States of Roraima (new record) and Amazonas. Material: One male subimago, BRAZIL, Roraima State, Caracara�� municipality, Serra da Mocidade, 01��42���21.3���N / 061��47���04.7���W, 03���04.ii. 2016, Nascimento, J.M.C. col. Pennsylvania trap., Published as part of Boldrini, R., 2017, Three new species of Miroculis from the Serra da Mocidade National Park, Roraima State, Brazil, with new records and checklist of the Leptophlebiidae (Ephemeroptera), pp. 573-583 in Zootaxa 4317 (3) on page 581, DOI: 10.11646/zootaxa.4317.3.8, http://zenodo.org/record/884837
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- 2017
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39. Terpides guyanensis Demoulin 1966
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Boldrini, R.
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Insecta ,Arthropoda ,Terpides guyanensis ,Leptophlebiidae ,Animalia ,Biodiversity ,Terpides ,Ephemeroptera ,Taxonomy - Abstract
Terpides guyanensis Demoulin, 1966 (Fig. 24) Distribution: Surinam and French Guyana. Brazil: State of Roraima. Material examined: Three nymphs, BRAZIL, Roraima State, Caracara�� municipality, Serra da Mocidade National Park, waterfall of the Pacu river, 01��36.060'N / 061��54548'W, 15���25.i. 2016, Boldrini, R., Barroso, P.C.S. and Xavier, F.F. col. (UFRR). Two nymphs, BRAZIL, Roraima State, Caracara��, Serra da Mocidade National Park, 01��42���19���N / 061��47���10���W, 0 1. ii. 2016, Nascimento, J.M.C. col. (INPA)., Published as part of Boldrini, R., 2017, Three new species of Miroculis from the Serra da Mocidade National Park, Roraima State, Brazil, with new records and checklist of the Leptophlebiidae (Ephemeroptera), pp. 573-583 in Zootaxa 4317 (3) on page 582, DOI: 10.11646/zootaxa.4317.3.8, http://zenodo.org/record/884837
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- 2017
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40. Mature and immature teratoma: A report from the second Italian pediatric study
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Terenziani, M., D'Angelo, P., Inserra, A., Boldrini, R., Bisogno, G., Babbo, G., Conte, M., Dall' Igna, P., De Pasquale, M., Indolfi, P., Piva, L., Riccipetitoni, G., Siracusa, F., Spreafico, L., Tamaro, P., Cecchetto, G., Terenziani, M, D'Angelo, P, Inserra, A, Boldrini, R, Bisogno, G, Babbo, GL, Conte, M, Dall' Igna, P, De Pasquale, MD, Indolfi, P, Piva, L, Riccipetitoni,G, Siracusa, F, Spreafico, L, Tamaro, P, and Cecchetto, G
- Subjects
immature teratoma ,Settore MED/20 - Chirurgia Pediatrica E Infantile ,Settore MED/20 ,germ cell tumor ,mature teratoma ,germ cell tumors ,childhood - Abstract
Background. Teratomas demonstrate a benign clinical behavior, however they may recur with malignant components or as teratoma, and in a small group of patients prognosis could be fatal. After the ®rst Italian study, we collected cases of teratoma, alongside the protocol for malignant germ cell tumors. Procedure. Patients with teratoma were collected from 2004 to 2014. Teratomas were classi®ed according to the WHO classi®cations, as mature and immature. Patients with pathological aFP and/or bHCG, and those with a malignant germ cell component were not included. Results. The study enrolled 219 patients (150 mature, 69 immature teratomas) with a median age at diagnosis of 42 months. The primary sites involved were: 118 gonadal and 101 extragonadal teratomas. Two females with ovarian teratoma had a positive family history. Complete and incomplete surgeries were performed in 85% and 9% of cases. Seventeen events occurred: six females had a second metachronous tumor (5 contralateral ovarian teratoma, 1 adrenal neuroblastoma) and 11 teratomas relapsed/progressed (3 mature, 8 immature teratomas). Two patients died, one of progressive immature teratoma and one of surgical complications. At a median follow up of 68 months, the event-free, relapse-free, and overall survival rates were 90.6%, 94.3%, 98.6%, respectively. Conclusions. Teratomas show a good prognosis, especially the mature ones: surgery and follow-up remain the standard approach. Incomplete surgery in immature teratoma is the group at greatest risk of relapse. Bilateral ovarian tumors are a possibility, and the rare family predisposition to ovarian mature teratoma warrants further analyses.
- Published
- 2015
41. Ichthyosis Prematurity Syndrome due to a Novel SLC27A4 Homozygous Mutation in an Italian Patient
- Author
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Diociaiuti, A, primary, Rosati, E, additional, Paglietti, M, additional, Vacca, P, additional, Boldrini, R, additional, Pisaneschi, E, additional, Castiglia, D, additional, Novelli, A, additional, and Hachem, M, additional
- Published
- 2018
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42. Paratesticular pilomatricoma: a new location
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Diomedi Camassei, F., Francalanci, P., Boldrini, R., Spagnoli, A., Lucchetti, M. C., and Ferro, F.
- Published
- 2001
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43. PD-L1 is a therapeutic target of the bromodomain inhibitor JQ1 and, combined with HLA class I, a promising prognostic biomarker in neuroblastoma
- Author
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Melaiu, O, Mina, M, Chierici, M, Boldrini, R, Jurman, G, Romania, P, D'Alicandro, V, Benedetti, MC, Castellano, A, Liu, T, Furlanello, C, Locatelli, F, Fruci, D, Melaiu, O, Mina, M, Chierici, M, Boldrini, R, Jurman, G, Romania, P, D'Alicandro, V, Benedetti, MC, Castellano, A, Liu, T, Furlanello, C, Locatelli, F, and Fruci, D
- Abstract
Purpose: This study sought to evaluate the expression of programmed cell death-ligand-1 (PD-L1) and HLA class I on neuroblastoma cells and programmed cell death-1 (PD-1) and lymphocyte activation gene 3 (LAG3) on tumor-infiltrating lymphocytes to better define patient risk stratification and understand whether this tumor may benefit from therapies targeting immune checkpoint molecules. Experimental Design: In situ IHC staining for PD-L1, HLA class I, PD-1, and LAG3 was assessed in 77 neuroblastoma specimens, previously characterized for tumor-infiltrating T-cell density and correlated with clinical outcome. Surface expression of PD-L1 was evaluated by flow cytometry and IHC in neuroblastoma cell lines and tumors genetically and/or pharmacologically inhibited for MYC and MYCN. A dataset of 477 human primary neuroblastomas from GEO and ArrayExpress databases was explored for PD-L1, MYC, and MYCN correlation. Results: Multivariate Cox regression analysis demonstrated that the combination of PD-L1 and HLA class I tumor cell density is a prognostic biomarker for predicting overall survival in neuroblastoma patients (P = 0.0448). MYC and MYCN control the expression of PD-L1 in neuroblastoma cells both in vitro and in vivo. Consistently, abundance of PD-L1 transcript correlates with MYC expression in primary neuroblastoma. Conclusions: The combination of PD-L1 and HLA class I represents a novel prognostic biomarker for neuroblastoma. Phar-macologic inhibition of MYCN and MYC may be exploited to target PD-L1 and restore an efficient antitumor immunity in high-risk neuroblastoma.
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- 2017
44. MYCN is an immunosuppressive oncogene dampening the expression of ligands for NK-cell-activating receptors in human high-risk neuroblastoma
- Author
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Brandetti, E., Veneziani, I., Melaiu, O., Pezzolo, A., Castellano, A., Boldrini, R., Ferretti, E., Fruci, D., Moretta, L., Pistoia, V., Locatelli, Franco, Cifaldi, L., Locatelli F. (ORCID:0000-0002-7976-3654), Brandetti, E., Veneziani, I., Melaiu, O., Pezzolo, A., Castellano, A., Boldrini, R., Ferretti, E., Fruci, D., Moretta, L., Pistoia, V., Locatelli, Franco, Cifaldi, L., and Locatelli F. (ORCID:0000-0002-7976-3654)
- Abstract
Neuroblastoma (NB) is the most common extracranial solid tumor occurring in childhood. Amplification of the MYCN oncogene is associated with poor prognosis. Downregulation on NB cells of ligands recognized by Natural Killer (NK) cell-activating receptors, involved in tumor cell recognition and lysis, may contribute to tumor progression and relapse. Here, we demonstrate that in human NB cell lines MYCN expression inversely correlates with that of ligands recognized by NKG2D and DNAM1 activating receptors in human NB cell lines. In the MYCN-inducible Tet-21/N cell line, downregulation of MYCN resulted in enhanced expression of the activating ligands MICA, ULBPs and PVR, which rendered tumor cells more susceptible to recognition and lysis mediated by NK cells. Conversely, a MYCN non-amplified NB cell line transfected with MYCN showed an opposite behavior compared with control cells. Consistent with these findings, an inverse correlation was detected between the expression of MYCN and that of ligands for NK-cell-activating receptors in 12 NB patient specimens both at mRNA and protein levels. Taken together, these results provide the first demonstration that MYCN acts as an immunosuppressive oncogene in NB cells that negatively regulates the expression of ligands for NKG2D and DNAM-1 NK-cell-activating receptors. Our study provides a clue to exploit MYCN expression levels as a biomarker to predict the efficacy of NK-cell-based immunotherapy in NB patients.
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- 2017
45. PD-L1 is a therapeutic target of the bromodomain inhibitor JQ1 and, combined with HLA class I, a promising prognostic biomarker in neuroblastoma
- Author
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Melaiu, O., Mina, M., Chierici, M., Boldrini, R., Jurman, G., Romania, P., D'Alicandro, V., Benedetti, M. C., Castellano, A., Liu, T., Furlanello, C., Locatelli, Franco, Fruci, D., Locatelli F. (ORCID:0000-0002-7976-3654), Melaiu, O., Mina, M., Chierici, M., Boldrini, R., Jurman, G., Romania, P., D'Alicandro, V., Benedetti, M. C., Castellano, A., Liu, T., Furlanello, C., Locatelli, Franco, Fruci, D., and Locatelli F. (ORCID:0000-0002-7976-3654)
- Abstract
Purpose: This study sought to evaluate the expression of programmed cell death-ligand-1 (PD-L1) and HLA class I on neuroblastoma cells and programmed cell death-1 (PD-1) and lymphocyte activation gene 3 (LAG3) on tumor-infiltrating lymphocytes to better define patient risk stratification and understand whether this tumor may benefit from therapies targeting immune checkpoint molecules. Experimental Design: In situ IHC staining for PD-L1, HLA class I, PD-1, and LAG3 was assessed in 77 neuroblastoma specimens, previously characterized for tumor-infiltrating T-cell density and correlated with clinical outcome. Surface expression of PD-L1 was evaluated by flow cytometry and IHC in neuroblastoma cell lines and tumors genetically and/or pharmacologically inhibited for MYC and MYCN. A dataset of 477 human primary neuroblastomas from GEO and ArrayExpress databases was explored for PD-L1, MYC, and MYCN correlation. Results: Multivariate Cox regression analysis demonstrated that the combination of PD-L1 and HLA class I tumor cell density is a prognostic biomarker for predicting overall survival in neuroblastoma patients (P = 0.0448). MYC and MYCN control the expression of PD-L1 in neuroblastoma cells both in vitro and in vivo. Consistently, abundance of PD-L1 transcript correlates with MYC expression in primary neuroblastoma. Conclusions: The combination of PD-L1 and HLA class I represents a novel prognostic biomarker for neuroblastoma. Phar-macologic inhibition of MYCN and MYC may be exploited to target PD-L1 and restore an efficient antitumor immunity in high-risk neuroblastoma.
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- 2017
46. Variations in multiple birth rates and impact on perinatal outcomes in Europe
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Heino, A. Gissler, M. Hindori-Mohangoo, A.D. Blondel, B. Klungsøyr, K. Verdenik, I. Mierzejewska, E. Velebil, P. Ólafsdóttir, H.S. Macfarlane, A. Zeitlin, J. Haidinger, G. Alexander, S. Pavlou, P. Mortensen, L. Sakkeus, L. Lack, N. Antsaklis, A. Berbik, I. Bonham, S. Cuttini, M. Misins, J. Jaselioniene, J. Wagener, Y. Gatt, M. Nijhuis, J. Van Der Pal, K. Klungsoyr, K. Szamotulska, K. Barros, H. Horga, M. Cap, J. Mandić, N.T. Bolúmar, F. Gottvall, K. Berrut, S. Klimont, J. Zhang, W.-H. Dramaix-Wilmet, M. Van Humbeeck, M. Leroy, C. Minsart, A.-F. Van Leeuw, V. Martens, E. De Spiegelaere, M. Verkruyssen, F. Willems, M. Aelvoet, W. Tafforeau, J. Renard, F. Walckiers, D. Cuignet, D. Demoulin, P. Cloots, H. Hendrickx, E. Kongs, A. Stylianou, D. Kyprianou, T. Skordes, N. Roos, J.L. Anderson, A.-M.N. Mortensen, L.H. Ritvanen, A. Colle, M.-H.B. Ego, A. Rey, G. Heller, G. Scharl, A. Drakakis, P. Bjarnadottir, R.I. Hardardóttir, H. Ragnarsdóttir, B. Stefánsdóttir, V. Haraldsdóttir, S. Mulligan, A. Tamburini, C. Boldrini, R. Prati, S. Loghi, M. Castagnaro, C. Marchetti, S. Burgio, A. Da Frè, M. Zile, I. Isakova, J. Gaidelyte, R. Jaselione, J. Billy, A. Touvrey-Lecomte, A. Van Der, K. De Bruin, P. Achterberg, P. Hukkelhoven, C. De Winter, G. Ravelli, A. Rijninks-Van Driel, G. Tamminga, P. Groesz, M. Elferink-Stinkens, P. Osen, A. Ebbing, M. Correia, S. Cucu, A. Novak-Antolič, Ž. Jane, M. Vidal, M.J. Barona, C. Mas, R. Alcaide, A.R. Lundqvist, E. König, C. Schmid, M. Dattani, N. Chalmers, J. Monteath, K. Climson, M. Marr, L. Gibson, R. Thomas, G. Osborne, R. Brown, R. Sweet, D. Evans, J. Magill, S. Graham, A. Reid, H. Falconer, T. McConnell, K. McComb, N. Euro-Peristat Scientific Committee
- Abstract
Objective: Infants from multiple pregnancies have higher rates of preterm birth, stillbirth and neonatal death and differences in multiple birth rates (MBR) exist between countries. We aimed to describe differences in MBR in Europe and to investigate the impact of these differences on adverse perinatal outcomes at a population level. Methods: We used national aggregate birth data on multiple pregnancies, maternal age, gestational age (GA), stillbirth and neonatal death collected in the Euro-Peristat project (29 countries in 2010, N = 5 074 643 births). We also used European Society of Human Reproduction and Embryology (ESHRE) data on assisted conception and single embryo transfer (SET). The impact of MBR on outcomes was studied using meta-analysis techniques with randomeffects models to derive pooled risk ratios (pRR) overall and for four groups of country defined by their MBR. We computed population attributable risks (PAR) for these groups. Results: In 2010, the average MBR was 16.8 per 1000 women giving birth, ranging from 9.1 (Romania) to 26.5 (Cyprus). Compared to singletons, multiples had a nine-fold increased risk (pRR 9.4, 95% Cl 9.1-9.8) of preterm birth (
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- 2016
47. Mediastinal Germ Cell Tumors in Pediatric Patients: A Report From the Italian Association of Pediatric Hematology and Oncology
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De Pasquale, Md, Crocoli, A, Conte, M, Indolfi, P, D'Angelo, P, Boldrini, R, Terenziani, M, and Inserra, A
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Settore MED/20 - Published
- 2016
48. Lumahyphes cocal Boldrini, Santos & Oliveira, 2015, sp. nov
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Boldrini, R., Santos, G. C., and Oliveira, D. R.
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Insecta ,Arthropoda ,Leptohyphidae ,Animalia ,Lumahyphes cocal ,Biodiversity ,Ephemeroptera ,Taxonomy ,Lumahyphes - Abstract
Lumahyphes cocal sp. nov. (Figs. 1–7) Diagnosis. Male imago: 1) vein CuP of fore wing with basal portion attached to vein A (Fig. 3 a,b); 2) terga base color whitish and terga I–X washed with gray, in whole or part (Figs. 1–2); 3) dorsal structure of penes apically rounded (Fig. 7); 4) spines of penes long, S-shaped in lateral view (Fig. 7); 5) spines of penes with apex directed anteriorly (Fig. 7). Description. Male imago. Antenna: 0.4 mm; body: 2.4–2.9 mm; fore wing: 2.8–2.9 mm; hind wing: 0.4–0.5 mm; tibia I: 0.8 mm; tibia II: 0.6 mm; tibia III: 0.7 mm; cerci broken and missing. Head (Figs. 1, 2). Dark grey dorsally, grey ventrally; compound eyes blackish; ocelli whitish, surrounded with blackish; antenna whitish and washed with gray. Thorax (Figs. 1, 2). Pronotum whitish washed with gray. Meso- and metanotum brown; prelateroscutum and scutellum washed with gray; medioscutum, submedioscutum, sublateroscutum and posterior scutal protuberance darker. Prosternum whitish and washed with light gray. Meso- and metasternum brown; basisternum darker. Legs yellowish light-brown, foreleg darker; coxa and trochanter brown; apex of femur with black mark on dorsal region (Fig. 1); base of tibia brown. Fore wing membrane greyish (Fig. 3); basal portion of vein CuP attached to vein A (Fig. 3 b); costal and subcostal regions gray. Hind wing membrane greyish (Fig. 4). Abdomen (Figs. 1, 2). Basal color whitish. Terga I–III & VIII–X washed with gray completely; terga IV–VII with only median region washed with gray. Sterna I–VI washed with gray; sternum IX washed with light brown. Genitalia (Figs. 5, 6, 7). Median projections of styliger plate short and pointed distally (Fig. 5); accessory dorsal structure of penes rounded (Fig. 7). Forceps whitish; segment I 0.75 × length of segment II; segment I washed with light brown. Penes whitish; spines washed with light brown; spines of penes S-curved in lateral view; apex of spines anteriorly directed (Fig. 7). Bases of cerci whitish. Etymology. The specific epithet of the new species is an allusion to the locality where the holotype was collected: Igarapé Cocal, Amajarí, Roraima. Distribution. BRAZIL: Roraima. Comments. Lumahyphes cocal sp. nov. shares most of the characteristics found in the diagnosis for the genus made by Domínguez et al. (2006). The only generic characterisitic that is not found in this new species is that vein CuP of the fore wing does not retains its basal portion paralleling CuA. In this new species, the basal portion of vein CuP on fore wing is attached to A (Fig. 3), as found in the genera Traverhyphes Molineri, 2001 and Vacupernius Wiersema & McCafferty, 2000. In the key by Dominguez et al. (2006), L. cocal sp. nov. would key as L. guacra or L. yagua. However, adults of these three species can be distinguished easily by the genitalia. Lumahyphes cocal sp. nov. has the accessory dorsal structure of penes and median projections of styliger plate not curved (Fig. 7); the spines of the penes are relatively long, S-curved in lateral view, and with anteriorly directed apices (Fig. 7). Lumahyphes guacra and L. yagua, on the other hand, have the dorsal structure and the inner posterolateral projection of styliger plate curved (as in Dominguez et al. 2006: Figs. 100 F, L), and the spines of the penes have a different shape in lateral view. Material examined. Holotype: Male imago, Brazil, Roraima, Amajari, Serra do Tepequém, Igarapé Cocal, 3 ° 73 'N, 61 ° 72 'W, 18.xii. 2014, Boldrini, R., Barroso, P.C.S., Oliveira, D.R. and Borges, R.L. leg. (INPA). Paratypes: Two male imagos, same data as holotype (INPA); four male imagos, same data as holotype, except, 01.xi. 2014, Boldrini, R., Boldrini, B.M.P.O., Barroso, P.C.S. and Santos, G.C. leg (CZNC); two male imagos (legs, wings and genitalia mounted on slides), same data as holotype, except, 01.xi. 2014, Boldrini, R., Boldrini, B.M.P.O., Barroso, P.C.S. and Santos, G.C. leg (UFRR); two male imagos, Brazil, Roraima, Pacaraima, Igarapé Samã, 4 ° 28 ' 30.8 ''N, 61 °09' 44.4 ''W, 06.i. 2015, Boldrini, R. leg. (UFRR).
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- 2015
- Full Text
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49. First record of the genus Lumahyphes Molineri, 2004 (Ephemeroptera: Leptohyphidae) from Brazil with description of a new species
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Boldrini, R., Santos, G. C., and Oliveira, D. R.
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Insecta ,Arthropoda ,Leptohyphidae ,Animalia ,Biodiversity ,Ephemeroptera ,Taxonomy - Abstract
Boldrini, R., Santos, G. C., Oliveira, D. R. (2015): First record of the genus Lumahyphes Molineri, 2004 (Ephemeroptera: Leptohyphidae) from Brazil with description of a new species. Zootaxa 4013 (1): 143-146, DOI: http://dx.doi.org/10.11646/zootaxa.4013.1.11
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- 2015
50. Novel PNPLA1 mutations in two Italian siblings with autosomal recessive congenital ichthyosis
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Diociaiuti, A., primary, Pisaneschi, E., additional, Zambruno, G., additional, Angioni, A., additional, Novelli, A., additional, Boldrini, R., additional, and El Hachem, M., additional
- Published
- 2017
- Full Text
- View/download PDF
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