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The propagation of sugarcane (Saccharum spp.) Requires seed with physiological and phytosanitary quality. The work was carried out with the objective of establishing a bank of sugarcane mother plants under semi-controlled conditions to guarantee the efficiency of in vitro propagation with plants free of Xanthomonas albilineans. A greenhouse was modified and the structure was remodeled, a Sarán shade mesh (50%) and a sprinkler irrigation system were placed. The plants were grown in plastic containers (20 m3 volume) with a mixture of cachaça compost and zeolite 3:1. Stems of cultivars C98-357, C97-445 and C86-156, without symptoms of leaf scald and with six months of culture, were cut into segments with a bud. Hydrothermal treatment was applied and subsequently the biostimulant VIUSID Agro® (0.8 ml l-1) before the buds planting. At three months of culture, a serological diagnosis was made to detect the presence of X. albilineans. The negative plants were treated with biostimulants and the response of the in vitro establishment was evaluated. Adequate plant growth was achieved under the conditions described. The establishment of a bank of sugarcane mother plants under semi-controlled conditions and its management guaranteed that microbial contamination and phenolic oxidation decreased in the in vitro establishment phase. The effectiveness of the hydrothermal treatment combined with the semi-controlled conditions and the management of the plants make it possible to reduce the incidence of X. albilineans. [ABSTRACT FROM AUTHOR]

FitoMas-E increases and accelerates the germination of the seeds, stimulates the development of roots, stems, leaves and improves the nutrition of plants. The objective of this work was to evaluate the effect of FitoMas-E on the in vitro rooting of sugarcane cultivars 'CP52-43', 'C87-51' and 'C1051-73'. A completely randomized experimental design was used with four treatments, three concentrations of FitoMas-E (0.5, 1.0 and 1.5 ml l-1) and indoleacetic acid (IAA) 1.3 mg l-1 as control in the liquid culture medium, with five repetition. At 15 days of culture the number of rooted shoots, height, leaves number and length of roots were evaluated. In the ex vitro acclimatization stage, its were quantified the number of plants with root, leaves and roots number per plant and it was measured the height after 45 days of transplanted. The results showed that the Fitomas-E in the cultivars 'CP52-43' and 'C87-51' achieved a number of shoots with a root similar to the control and in 'C1051-73' it exceeded it with the concentration of 0.5 ml l-1 of this biostimulant. Leaf height, leaves number and root length reached values significantly higher than control, with 1.0 ml l-1 phytostimulant, except for 'CP52-43' where similar root length was achieved among treatments. In the ex vitro acclimatization stage the in vitro plants rooted with the FitoMas-E achieved the required quality parameters, exceeding the controls for the variables height, number of roots and length of the longest root. Among genotypes there were significant differences for all cultivars. [ABSTRACT FROM AUTHOR]

The effect of three doses of Fitomas–E (0.5, 1.0 and 1.5 ml. L-1 of culture medium) in comparison with the Indole Acetic Acid (IAA) on the rooting and later acclimatization of sugarcane cultivar C95-414 was studied. The evaluated variables were Percentage of rooted plants, number of roots, longitude of the stalk, the root and the leaf +1, number of active leaves and fresh and dry mass of the plant. In the acclimatization stage, the formation of the root ball was also assessed. The results in the rooting stage showed that the treatments with Fitomas–E were always, from the statistical point of view, in the first place in the percentage of rooted plants, longitude of the plant and of the leaf +1, being the best dose 1.0 ml of Fitomas–E per liter of culture medium. The rest of the variables did not show statistical differences among treatments. In the acclimatization stage, the plants that were treated with Fitomas-E did not show statistical differences among the doses of the bioestimulating applied in none of the evaluated parameters, but they did showed statistically positive results when comparing with the standard. These results suggest the convenience of substituting the IAA by Fitomas– E in the rooting stage of sugarcane cultivars as an alternative in the substitution of imports, when added at the culture medium at a rate of 1.0 ml. L-1 of the bioestimulating. [ABSTRACT FROM AUTHOR]

Seed Banks and agricultural farms benefit greatly from plant in vitro Culture techniques, for their rapid propagation processes and the production of desease-free plantlets. However, the production and commercialization process can fail and it is not as successfull as it was supposed to. The objective of this study is to identify potential failure modes at the acclimatiztion stage of plantlets to ex vitro conditions at Estación Territorial de Investigaciones de la Caña de Azúcar de Villa Clara (ETICA). A methodology of Failure Mode and Effects Analysis (FMEA) was applied. A value of 80 or higher was determined to be the Risk Priority Number (RPN) and the more relevant failure modes were defined at the acclimatization and commercialization stages and ground rules and assumptions were established in order to prevent and control failure modes of the process. The study and implementation of the methodology to other processes at ETICA is strongly recommended. [ABSTRACT FROM AUTHOR]

The objective of the work was establishing the requirements of quality in in vitro sugarcane plantlets in the adaptation stage aimed to determine the nominal values of the characteristics of substitute quality for this crop. It was used the cultivar C86-51, after the rooting stage, being the one that was in existence in that period. The percentage of survival and loss was determined, coinciding with the transplant stage to the field where the greatest percentage of survival was reached by in vitro plantlets with size from 5 to 7 cm. To develop this work tools of quality like interviews, surveys, experts' method and brain storming applying statisticians that determined the defined characteristics for the realization of the study. It was recommended to make extensive the results to all the Tissue Culture Plants of the country and to make a diagnosis of quality that allows establishing a procedure for all the crops to market. [ABSTRACT FROM AUTHOR]