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Your search keyword '"Bautor, Jaqueline"' showing total 38 results
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38 results on '"Bautor, Jaqueline"'

5. Arabidopsis PHYTOALEXIN DEFICIENT 4 promotes the maturation and nuclear accumulation of immune-related cysteine protease RD19.

Author

Zeng, Yanhong, Zheng, Zichao, Hessler, Giuliana, Zou, Ke, Leng, Junchen, Bautor, Jaqueline, Stuttmann, Johannes, Xue, Li, Parker, Jane E, and Cui, Haitao

Subjects
PHYTOALEXINS, CYSTEINE, ARABIDOPSIS, PSEUDOMONAS syringae, DISEASE resistance of plants
Abstract

Arabidopsis PHYTOALEXIN DEFICIENT 4 (PAD4) has an essential role in pathogen resistance as a heterodimer with ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1). Here we investigated an additional PAD4 role in which it associates with and promotes the maturation of the immune-related cysteine protease RESPONSIVE TO DEHYDRATION 19 (RD19). We found that RD19 and its paralog RD19c promoted EDS1- and PAD4-mediated effector-triggered immunity to an avirulent Pseudomonas syringae strain, DC3000, expressing the effector AvrRps4 and basal immunity against the fungal pathogen Golovinomyces cichoracearum. Overexpression of RD19, but not RD19 protease-inactive catalytic mutants, in Arabidopsis transgenic lines caused EDS1- and PAD4-dependent autoimmunity and enhanced pathogen resistance. In these lines, RD19 maturation to a pro-form required its catalytic residues, suggesting that RD19 undergoes auto-processing. In transient assays, PAD4 interacted preferentially with the RD19 pro-protease and promoted its nuclear accumulation in leaf cells. Our results lead us to propose a model for PAD4-stimulated defense potentiation. PAD4 promotes maturation and nuclear accumulation of processed RD19, and RD19 then stimulates EDS1–PAD4 dimer activity to confer pathogen resistance. This study highlights potentially important additional PAD4 functions that eventually converge on canonical EDS1–PAD4 dimer signaling in plant immunity. [ABSTRACT FROM AUTHOR]

Published
2024
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6. Arabidopsis Topless-related 1 mitigates physiological damage and growth penalties of induced immunity

Author

Griebel, Thomas, Lapin, Dmitry, Locci, Federica, Kracher, Barbara, Bautor, Jaqueline, Concia, Lorenzo, Benhamed, Moussa, Parker, Jane E., Sub Translational Plant Biology, and Translational Plant Biology

Subjects
ChIP-Seq, 500 Naturwissenschaften und Mathematik::580 Pflanzen (Botanik)::580 Pflanzen (Botanik), Physiology, corepressor, Plant Science, Topless-related 1, immunity, resilience
Abstract

Transcriptional corepressors of the Topless (TPL) family regulate plant hormone and immunity signaling. The lack of a genome-wide profile of their chromatin associations limits understanding of the TPL family roles in transcriptional regulation. Chromatin immunoprecipitation with sequencing (ChIP-Seq) was performed on Arabidopsis thaliana lines expressing GFP-tagged Topless-related 1 (TPR1-GFP) with and without constitutive immunity via Enhanced Disease Susceptibility 1 (EDS1). RNA-Seq profiling of the TPR1-GFP lines and pathogen-infected tpl/tpr mutants, combined with measuring immunity, growth, and physiological parameters was employed to investigate TPL/TPR roles in immunity and defense homeostasis. TPR1 was enriched at promoter regions of c. 1400 genes and c. 10% of the detected binding required EDS1 immunity signaling. In a tpr1 tpl tpr4 (t3) mutant, resistance to bacteria was slightly compromised, and defense-related transcriptional reprogramming was weakly reduced or enhanced, respectively, at early (< 1 h) and late 24 h stages of bacterial infection. The t3 plants challenged with bacteria or pathogen-associated molecular pattern nlp24 displayed photosystem II dysfunctions. Also, t3 plants were hypersensitive to phytocytokine pep1 at the level of root growth inhibition. Transgenic expression of TPR1 rescued these t3 physiological defects. We propose that TPR1 and TPL family proteins function in Arabidopsis to reduce detrimental effects associated with activated transcriptional immunity.

Published
2023

9. Arabidopsis Topless-related 1 mitigates physiological damage and growth penalties of induced immunity

Author

Sub Translational Plant Biology, Translational Plant Biology, Griebel, Thomas, Lapin, Dmitry, Locci, Federica, Kracher, Barbara, Bautor, Jaqueline, Concia, Lorenzo, Benhamed, Moussa, Parker, Jane E, Sub Translational Plant Biology, Translational Plant Biology, Griebel, Thomas, Lapin, Dmitry, Locci, Federica, Kracher, Barbara, Bautor, Jaqueline, Concia, Lorenzo, Benhamed, Moussa, and Parker, Jane E

Published
2023

13. Corrigendum to: A coevolved EDS1-SAG101-NRG1 module mediates cell death signaling by TIR-domain immune receptors

Author

Lapin, Dmitry, Kovacova, Viera, Sun, Xinhua, Dongus, Joram A., Bhandari, Deepak, von Born, Patrick, Bautor, Jaqueline, Guarneri, Nina, Rzemieniewski, Jakub, Stuttmann, Johannes, Beyer, Andreas, and Parker, Jane E.

Subjects
Plant Science, Cell Biology
Abstract

This is a correction to: The Plant Cell, Volume 31, Issue 10, October 2019, Pages 2430–2455, https://doi-org.proxy.library.uu.nl/10.1105/tpc.19.00118

Published
2021

15. Arabidopsis Topless-related 1 mitigates physiological damage and growth penalties of induced immunity

Author

Griebel, Thomas, Lapin, Dmitry, Locci, Federica, Kracher, Barbara, Bautor, Jaqueline, Qiu, Jingde, Concia, Lorenzo, Benhamed, Moussa, Parker, Jane E., Griebel, Thomas, Lapin, Dmitry, Locci, Federica, Kracher, Barbara, Bautor, Jaqueline, Qiu, Jingde, Concia, Lorenzo, Benhamed, Moussa, and Parker, Jane E.

Abstract

Transcriptional corepressors of the Topless family are important regulators of plant hormone and immunity signaling. The lack of a genome-wide profile of their chromatin associations limits understanding of transcriptional regulation in plant immune responses. Chromatin immunoprecipitation with sequencing (ChIP-seq) was performed on GFP-tagged Topless-related 1 (TPR1) expressed in Arabidopsis thaliana lines with and without constitutive immunity dependent on Enhanced Disease Susceptibility 1 (EDS1). RNA-seq profiling of pathogen-infected tpl/tpr mutants and assessments of growth and physiological parameters were employed to determine TPL/TPR roles in transcriptional immunity and defense homeostasis. TPR1 bound to promoter regions of ~1,400 genes and ~10% of the detected binding required EDS1 immunity signaling. A tpr1 tpl tpr4 (t3) mutant displayed mildly enhanced defense-related transcriptional reprogramming upon bacterial infection but not increased bacterial resistance. Bacteria or pep1 phytocytokine-challenged t3 plants exhibited, respectively, photosystem II dysfunction and exacerbated root growth inhibition. Transgenic expression of TPR1 restored the t3 physiological defects. We propose that TPR1 and TPL-family proteins function in Arabidopsis to reduce detrimental effects associated with activated transcriptional immunity.

Published
2021

16. Arabidopsis Topless-related 1 mitigates physiological damage and growth penalties of induced immunity

Author

Sub Plant-Microbe Interactions, Plant Microbe Interactions, Griebel, Thomas, Lapin, Dmitry, Locci, Federica, Kracher, Barbara, Bautor, Jaqueline, Qiu, Jingde, Concia, Lorenzo, Benhamed, Moussa, Parker, Jane E., Sub Plant-Microbe Interactions, Plant Microbe Interactions, Griebel, Thomas, Lapin, Dmitry, Locci, Federica, Kracher, Barbara, Bautor, Jaqueline, Qiu, Jingde, Concia, Lorenzo, Benhamed, Moussa, and Parker, Jane E.

Published
2021

17. Pathogen effector recognition-dependent association of NRG1 with EDS1 and SAG101 in TNL receptor immunity

Author

Sub Plant-Microbe Interactions, Plant Microbe Interactions, Sun, Xinhua, Lapin, Dmitry, Feehan, Joanna M., Stolze, Sara C., Kramer, Katharina, Dongus, Joram A., Rzemieniewski, Jakub, Blanvillain-Baufumé, Servane, Harzen, Anne, Bautor, Jaqueline, Derbyshire, Paul, Menke, Frank L.H., Finkemeier, Iris, Nakagami, Hirofumi, Jones, Jonathan D.G., Parker, Jane E., Sub Plant-Microbe Interactions, Plant Microbe Interactions, Sun, Xinhua, Lapin, Dmitry, Feehan, Joanna M., Stolze, Sara C., Kramer, Katharina, Dongus, Joram A., Rzemieniewski, Jakub, Blanvillain-Baufumé, Servane, Harzen, Anne, Bautor, Jaqueline, Derbyshire, Paul, Menke, Frank L.H., Finkemeier, Iris, Nakagami, Hirofumi, Jones, Jonathan D.G., and Parker, Jane E.

Published
2021

20. Pathogen effector recognition-dependent association of NRG1 with EDS1 and SAG101 in TNL receptor immunity

Author

Sun, Xinhua, primary, Lapin, Dmitry, additional, Feehan, Joanna M., additional, Stolze, Sara C., additional, Kramer, Katharina, additional, Dongus, Joram A., additional, Rzemieniewski, Jakub, additional, Blanvillain-Baufumé, Servane, additional, Harzen, Anne, additional, Bautor, Jaqueline, additional, Derbyshire, Paul, additional, Menke, Frank L. H., additional, Finkemeier, Iris, additional, Nakagami, Hirofumi, additional, Jones, Jonathan D.G., additional, and Parker, Jane E., additional

Published
2020
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30. Functional Analysis of Hyaloperonospora arabidopsidis RXLR Effectors

Author

Pel, Michiel J C, Wintermans, Paul C A, Cabral, Adriana, Robroek, Bjorn J M, Seidl, Michael F, Bautor, Jaqueline, Parker, Jane E, Van den Ackerveken, Guido, Pieterse, Corné M J, Sub Plant-Microbe Interactions, Sub Ecology and Biodiversity, Sub Theoretical Biology & Bioinformatics, Ecology and Biodiversity, Plant Microbe Interactions, Theoretical Biology and Bioinformatics, Sub Plant-Microbe Interactions, Sub Ecology and Biodiversity, Sub Theoretical Biology & Bioinformatics, Ecology and Biodiversity, Plant Microbe Interactions, and Theoretical Biology and Bioinformatics

Subjects
0106 biological sciences, Science, Plant Pathogens, Arabidopsis, Virulence, Plant Science, Biology, Plant Genetics, Bioinformatics, Microbiology, 01 natural sciences, 03 medical and health sciences, Plant Microbiology, Genetics, Pseudomonas syringae, Plant defense against herbivory, Plant Defenses, Plant Immunity, Gene, Plant Diseases, Plant Proteins, 030304 developmental biology, 0303 health sciences, Hyaloperonospora arabidopsidis, Multidisciplinary, Effector, Biology and Life Sciences, Plant Disease Resistance, Plant Pathology, biology.organism_classification, Plant disease, Oomycetes, Plant Physiology, Host-Pathogen Interactions, Medicine, Research Article, 010606 plant biology & botany
Abstract

The biotrophic plant pathogen Hyaloperonospora arabidopsidis produces a set of putative effector proteins that contain the conserved RXLR motif. For most of these RXLR proteins the role during infection is unknown. Thirteen RXLR proteins from H. arabidopsidis strain Waco9 were analyzed for sequence similarities and tested for a role in virulence. The thirteen RXLR proteins displayed conserved N-termini and this N-terminal conservation was also found in the 134 predicted RXLR genes from the genome of H. arabidopsidis strain Emoy2. To investigate the effects of single RXLR effector proteins on plant defense responses, thirteen H. arabidopsidis Waco9 RXLR genes were expressed in Arabidopsis thaliana. Subsequently, these plants were screened for altered susceptibility to the oomycetes H. arabidopsidis and Phytophthora capsici, and the bacterial pathogen Pseudomonas syringae. Additionally, the effect of the RXLR proteins on flg22-triggered basal immune responses was assessed. Multifactorial analysis of results collated from all experiments revealed that, except for RXLR20, all RXLR effector proteins tested affected plant immunity. For RXLR9 this was confirmed using a P. syringae ΔCEL-mediated effector delivery system. Together, the results show that many H. arabidopsidis RXLR effectors have small effects on the plant immune response, suggesting that suppression of host immunity by this biotrophic pathogen is likely to be caused by the combined actions of effectors.

Published
2014
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31. A core function of EDS1 with PAD4 is to protect the salicylic acid defense sector in Arabidopsis immunity.

Author

Cui, Haitao, Gobbato, Enrico, Kracher, Barbara, Qiu, Jingde, Bautor, Jaqueline, and Parker, Jane E.

Subjects
ARABIDOPSIS thaliana, PLANT defenses, PLANT ecology, PLANT RNA, RNA sequencing
Abstract

Plant defenses induced by salicylic acid (SA) are vital for resistance against biotrophic pathogens. In basal and receptor-triggered immunity, SA accumulation is promoted by Enhanced Disease Susceptibility1 with its co-regulator Phytoalexin Deficient4 (EDS1/PAD4). Current models position EDS1/PAD4 upstream of SA but their functional relationship remains unclear., In a genetic and transcriptomic analysis of Arabidopsis autoimmunity caused by constitutive or conditional EDS1/PAD4 overexpression, intrinsic EDS1/PAD4 signaling properties and their relation to SA were uncovered., A core EDS1/PAD4 pathway works in parallel with SA in basal and effector-triggered bacterial immunity. It protects against disabled SA-regulated gene expression and pathogen resistance, and is distinct from a known SA-compensatory route involving MAPK signaling. Results help to explain previously identified EDS1/PAD4 regulated SA-dependent and SA-independent gene expression sectors., Plants have evolved an alternative route for preserving SA-regulated defenses against pathogen or genetic perturbations. In a proposed signaling framework, EDS1 with PAD4, besides promoting SA biosynthesis, maintains important SA-related resistance programs, thereby increasing robustness of the innate immune system. [ABSTRACT FROM AUTHOR]

Published
2017
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32. Functional Analysis of Hyaloperonospora arabidopsidis RXLR Effectors

Author

Sub Plant-Microbe Interactions, Sub Ecology and Biodiversity, Sub Theoretical Biology & Bioinformatics, Ecology and Biodiversity, Plant Microbe Interactions, Theoretical Biology and Bioinformatics, Pel, Michiel J C, Wintermans, Paul C A, Cabral, Adriana, Robroek, Bjorn J M, Seidl, Michael F, Bautor, Jaqueline, Parker, Jane E, Van den Ackerveken, Guido, Pieterse, Corné M J, Sub Plant-Microbe Interactions, Sub Ecology and Biodiversity, Sub Theoretical Biology & Bioinformatics, Ecology and Biodiversity, Plant Microbe Interactions, Theoretical Biology and Bioinformatics, Pel, Michiel J C, Wintermans, Paul C A, Cabral, Adriana, Robroek, Bjorn J M, Seidl, Michael F, Bautor, Jaqueline, Parker, Jane E, Van den Ackerveken, Guido, and Pieterse, Corné M J

Published
2014

33. Analysis of a Plant Complex Resistance Gene Locus Underlying Immune-Related Hybrid Incompatibility and Its Occurrence in Nature

Author

Alcazar, Ruben, von Reth, Marcel, Bautor, Jaqueline, Chae, Eunyoung, Weigel, Detlef, Koornneef, Maarten, Parker, Jane E., Alcazar, Ruben, von Reth, Marcel, Bautor, Jaqueline, Chae, Eunyoung, Weigel, Detlef, Koornneef, Maarten, and Parker, Jane E.

Abstract

Mechanisms underlying speciation in plants include detrimental (incompatible) genetic interactions between parental alleles that incur a fitness cost in hybrids. We reported on recessive hybrid incompatibility between an Arabidopsis thaliana strain from Poland, Landsberg erecta (Ler), and many Central Asian A. thaliana strains. The incompatible interaction is determined by a polymorphic cluster of Toll/interleukin-1 receptor-nucleotide binding-leucine rich repeat (TNL) RPP1 (Recognition of Peronospora parasitica1)-like genes in Ler and alleles of the receptor-like kinase Strubbelig Receptor Family 3 (SRF3) in Central Asian strains Kas-2 or Kond, causing temperature-dependent autoimmunity and loss of growth and reproductive fitness. Here, we genetically dissected the RPP1-like Ler locus to determine contributions of individual RPP1-like Ler (R1-R8) genes to the incompatibility. In a neutral background, expression of most RPP1-like Ler genes, except R3, has no effect on growth or pathogen resistance. Incompatibility involves increased R3 expression and engineered R3 overexpression in a neutral background induces dwarfism and sterility. However, no individual RPP1-like Ler gene is sufficient for incompatibility between Ler and Kas-2 or Kond, suggesting that co-action of at least two RPP1-like members underlies this epistatic interaction. We find that the RPP1-like Ler haplotype is frequent and occurs with other Ler RPP1-like alleles in a local population in Gorzow Wielkopolski (Poland). Only Gorzow individuals carrying the RPP1-like Ler haplotype are incompatible with Kas-2 and Kond, whereas other RPP1-like alleles in the population are compatible. Therefore, the RPP1-like Ler haplotype has been maintained in genetically different individuals at a single site, allowing exploration of forces shaping the evolution of RPP1-like genes at local and regional population scales.

Published
2014

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