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19 results on '"Amin, Shilpa"'

3. Temporal Evaluation of a Minimally Invasive Method of Preimplantation Genetic Testing for Aneuploidy (mi-PGT-A) in Human Embryos.

Author

Phillips, Katharine R. B., Kuzma-Hunt, Alexander G., Neal, Michael S., Lisle, Connie, Sribalachandran, Hariharan, Carter, Ronald F., Amin, Shilpa, Karnis, Megan F., and Faghih, Mehrnoosh

Subjects
DNA analysis, EMBRYOS, SPOUSES, MINIMALLY invasive procedures, PREIMPLANTATION genetic diagnosis, ANEUPLOIDY, CULTURE media (Biology), DESCRIPTIVE statistics, NUCLEIC acids, EXTRACELLULAR space, BLASTULA, CONFIDENCE intervals, GENETIC testing, SEQUENCE analysis, BLASTOMERES
Abstract

Preimplantation genetic testing for aneuploidy (PGT-A) has become a useful approach for embryo selection following IVF and ICSI. However, the biopsy process associated with PGT-A is expensive, prone to errors in embryo ploidy determination, and potentially damaging, impacting competence and implantation potential. Therefore, a less invasive method of PGT-A would be desirable and more cost-effective. Noninvasive methods for PGT-A (ni-PGT-A) have been well-studied but present limitations in terms of cf-DNA origin and diagnostic accuracy. Minimally invasive pre-implantation genetic testing (mi-PGT-A) for frozen-thawed embryo transfer is a promising, less studied approach that utilizes a combination of spent culture media (SCM) and blastocoelic fluid (BF)-derived cell-free (CF)-DNA for genetic testing. This study aimed to optimize the effectiveness of mi-PGT-A for aneuploidy diagnosis by investigating the optimal temporal sequence for this protocol. SCM+BF was collected at either 48 or 72 h of culture after thawing day 3 preimplantation embryos. cf-DNA in the SCM+BF was amplified, analyzed by next-generation sequencing (NGS) and compared with results from the corresponding whole embryos (WEs) obtained from human embryos donated for research. Fifty-three (42 expanded blastocysts, 9 early blastocysts, and 2 morula) WE and SCM+BF samples were analyzed and compared. The overall concordance rate between SCM+BF and WE was 60%. Gender and ploidy concordance improved with extended culture time from 48 h (73% and 45%) to 72 h (100% and 64%), respectively. These results demonstrate that SCM+BF-derived cf-DNA can be successfully used for mi-PGT-A. Our findings indicate that longer embryo culture time prior to SCM+BF-derived cf-DNA analysis improves DNA detection rate and concordance with WEs and decreases the proportion of false positive results. [ABSTRACT FROM AUTHOR]

Published
2024
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10. Contributors

Author

Abdou, Nabih I., primary, Abel, Kathryn M., additional, Aberg, Judith A., additional, Ackard, Diann M., additional, Adachi, Jonathan D., additional, Addesa, Jill Aimee, additional, Agate, Robert J., additional, Allen, Jerilyn, additional, Amin, Shilpa H., additional, Anastasiadis, Aristotelis G., additional, Anderson, David E., additional, Aranoff, Gaya, additional, Arnold, Arthur P., additional, Atwood, Craig S., additional, Balmaceda, Casilda, additional, Banerjee, Promila, additional, Barkin, Jamie S., additional, Bassuk, Shari S., additional, Bateman, David, additional, Becker, Carolyn, additional, Bell, Jennifer, additional, Bies, Robert R., additional, Bigos, Kristin L., additional, Bilello, Kathryn L., additional, Bilezikian, John P., additional, Bjornson, Candice, additional, Blaivas, Jerry G., additional, Blumenthal, Roger S., additional, Bouillon, Roger, additional, Bowen, Richard, additional, Brill, Kimberly T., additional, Burkman, Ronald T., additional, Byne, William, additional, Callahan, Leigh Ann, additional, Canto, Marcia Irene, additional, Carruth, Laura L., additional, Castell, Donald O., additional, Chang, Lin, additional, Chen, Min C., additional, Chesney, Margaret A., additional, Chiasson, Mary Ann, additional, Chue, Pierre, additional, Chung, Pak, additional, Chung, Wendy K., additional, Chutka, Darryl S., additional, Cocilovo, Costanza, additional, Collaer, Marcia L., additional, Conjeevaram, Hari S., additional, Coombs, Robert W., additional, Coulston, Ann M., additional, Cranney, Ann, additional, Cruz-Correa, Marcia, additional, D'Ambrosio, Carolyn, additional, Dardano, Kristin L., additional, Das, Sai Krupa, additional, Daugherty, L. Eugene, additional, Davis, Anne R., additional, Dawes, Lillian G., additional, Demark-Wahnefried, Wendy, additional, DeMeo, Dawn L., additional, Despommier, Dickson D., additional, Douglas, Pamela S., additional, Droggin, Dmitry, additional, DuBeau, Catherine E., additional, Duncan, Alison M., additional, Early, Dayna, additional, El-Sadr, Wafaa, additional, Erbella, Jose, additional, Evans, William S., additional, Fleming, Kevin C., additional, Flisser, Adam J., additional, Fogelman, David, additional, Ford, Gordon, additional, Fox, Susan C., additional, Foxx-Orenstein, Amy, additional, Frederiksen, Marilynn C., additional, Garvin, James H., additional, Gearhart, John P., additional, Ginsberg, Claudia L., additional, Goldstein, Marc, additional, Gur, Raquel E., additional, Gur, Ruben C., additional, Haller, Christine A., additional, Hammer, Scott M., additional, Hartmann, Lynn C., additional, Hay, Christine M., additional, Haymart, Megan Rist, additional, Heitkemper, Margaret M., additional, Hershman, Dawn, additional, Hogan, Daniel L., additional, Hopps, Carin V., additional, Huang, Shiew-Mei, additional, Jacobson, Stacy D., additional, Joseph, James, additional, Kammer, Gary M., additional, Karlstadt, Robyn G., additional, Karnam, Umaprasanna S., additional, Kashyap, Sonya, additional, Kaufman, David M., additional, Kayser, Steven R., additional, Khosla, Sundeep, additional, Kirmani, Nigar, additional, Knopman, David, additional, Kolevska, Tatjana, additional, Kolonel, Laurence N., additional, Kuhle, Carol L., additional, Kurzer, Mindy S., additional, Lahita, Robert G., additional, Lazarus, George M., additional, Lee, Susan J., additional, Legato, Marianne J., additional, Legha, Jaswinder K., additional, Lesko, Lawrence J., additional, Levine, Jon D., additional, Loh, Li-Ming, additional, Looker, Anne C., additional, Lowy, Franklin D., additional, Mallik, Susmita, additional, Manson, JoAnn E., additional, Marcus, Dawn A., additional, Martin, Antonio, additional, Matthay, Richard A., additional, McClelland, R. Scott, additional, Mercurio, Mary Gail, additional, Metzl, Jordan D., additional, Miaskowski, Christine, additional, Miller, Margaret, additional, Miller, Paul D., additional, Milsom, Jeffrey W., additional, Mitchell, Ian, additional, Moncher, Karen L., additional, Moores, Lisa, additional, Morrell, Martha J., additional, Murin, Susan, additional, Nass, Caitlin M., additional, Neugut, Alfred I., additional, Nichols, Gwen L., additional, O'Loughlin, Colm J., additional, Ong, Albert M., additional, Ordovas, Jose M., additional, O'Reilly, Katherine M.A., additional, Papadopoulos, Kyriakos, additional, Papaioannou, Alexandra, additional, Parke, Ann L., additional, Perry, George, additional, Pham, Thai, additional, Phipps, William R., additional, Pietropaoli, Anthony P., additional, Pollock, Bruce G., additional, Powderly, William G., additional, Pratha, Vijaya S., additional, Proctor, Deborah Denise, additional, Pruthi, Sandhya, additional, Ramsden, Timothy J., additional, Reddy, Sarathchandra I., additional, Rider, Virginia, additional, Ritchie, Ellen, additional, Roberts, Barbara H., additional, Roberts, Susan B., additional, Rock, Cheryl L., additional, Romanzi, Lauri, additional, Rosano, Giuseppe M.C., additional, Rose, Melissa, additional, Rosen, Michael R., additional, Rosen, Tove S., additional, Rosner, Zachary, additional, Rossi, Jennifer, additional, Rubin, Mishaela R., additional, Ruffin, Mack T., additional, Russo, Donna, additional, Sahajwalla, Chandra, additional, Salomon, Laurent, additional, Sanfey, Hilary, additional, Sarrel, Philip M., additional, Schlegel, Peter N., additional, Schwartz, Janice B., additional, Seeman, Mary V., additional, Segarra, Annabell C., additional, Sekaer, Christina, additional, Selleck, Meredith, additional, Shabsigh, Ridwan, additional, Sheares, Beverley J., additional, Shoupe, Donna, additional, Shulman, Lee P., additional, Silverman, Edwin K., additional, Sime, Patricia J., additional, Smith, Mark A., additional, Sobieszczyk, Magdalena E., additional, Sonoda, Toyooki, additional, Stanford, Edward J., additional, Stein, Donald G., additional, Sullivan, Richard C., additional, Supinski, Gerald, additional, Tanios, Maged, additional, Tarnopolsky, Mark A., additional, Temple, Robert, additional, Tiersten, Amy, additional, Toigo, Theresa, additional, Tory, Heather O., additional, Trawick, David R., additional, Tsiouris, Simon J., additional, Tungsiripat-Gerber, Marisa, additional, Vaccarino, Viola, additional, Valkenburgh, Mark C., additional, Vanderschueren, Dirk, additional, Veldhuis, Johannes D., additional, Venken, Katrien, additional, Walker, Sara E., additional, Weisfeldt, Myron L., additional, Weiss, Jeffrey P., additional, Wilkin, Timothy, additional, Wolf, Jacqueline L., additional, Woodhouse, C.R.J., additional, Yin, Michael, additional, Zeana, Cosmina, additional, and Zojwalla, Naseem, additional

Published
2004
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16. Tennessee Medicare Plans in 2020.

Author

Amin, Shilpa and Jerusalimiec, Vanessa

Subjects
MEDICARE, PLANNING
Abstract

The article informs that Medicare in Tennessee can provide comprehensive health insurance coverage, and can enroll in Medicare Tennessee plans. Topics include enroll in original Medicare in Tennessee, add drug coverage, or even switch to an Advantage plan; and covers inpatient hospital care, short-term nursing facility care, and home health care.

Published
2020

17. Controlled Ovarian Hyperstimulation with Intrauterine Insemination Is More Successful After r-hCG Administration Than Spontaneous LH Surge.

Author

Taerk, Evan, Hughes, Edward, Greenberg, Cassandra, Neal, Michael, Amin, Shilpa, Faghih, Mehrnoosh, and Karnis, Megan

Subjects
*HUMAN artificial insemination, *CHI-squared test, *CHORIONIC gonadotropins, *FISHER exact test, *FOLLICLE-stimulating hormone, *INFERTILITY, *LUTEINIZING hormone, *T-test (Statistics), *LOGISTIC regression analysis, *OVARIAN hyperstimulation syndrome
Abstract

Background: The purpose of this study was to evaluate whether clinical pregnancy rate is affected by timing intrauterine insemination (IUI) according to serum LH surge, r-hCG trigger, or a combination of LH surge and r-hCG trigger in controlled ovarian hyperstimulation (COH) cycles for patients with a variety of infertility etiologies. Methods: The last 365 consecutive COH-IUI cycles performed at ONE Fertility Burlington in 2014 were reviewed and categorized according to method of IUI timing. Associations between categorical variables were analyzed using a combination of Chi-square and Fisher's Exact tests, and between continuous variables using independent sample t-tests and logistic regression to a level of significance of p<0.05. Results: The overall clinical pregnancy rate in this sample was 18.1% (66/365). Administration of r-hCG prior to IUI resulted in a higher clinical pregnancy rate compared with spontaneous serum LH surge: 18.2% vs. 5.8%, p=0.012. Patients in whom r-hCG was administered concomitantly with a serum LH surge had a higher clinical pregnancy than the r-hCG trigger group (30.8% vs. 18.2%, p=0.004) and LH surge group (30.8% vs. 5.8%, p<0.001). A sub-group analysis revealed that patients receiving r-FSH, rather than clomiphene or letrozole, had a significantly higher clinical pregnancy rate after r-hCG trigger as compared to the LH surge group (21.7% vs. 2.1%, p=0.01). Conclusion: In subfertile couples undergoing COH-IUI, r-hCG administration was associated with an increased clinical pregnancy rate compared with spontaneous serum LH surge. When r-hCG was administered concomitantly with a serum LH surge, this benefit was amplified. The effect appears to be of particular importance in r-FSH-medicated cycles. [ABSTRACT FROM AUTHOR]

Published
2017

18. Importance of Assessing Musculoskeletal Disease in Older Women.

Author

Rothschild, Bruce M., Fitzpatrick, Lorraine A., Kuhle, Carol L., and Amin, Shilpa H.

Subjects
*MUSCULOSKELETAL diseases in old age, *OSTEOPOROSIS, *OSTEOARTHRITIS, *OLDER women
Abstract

Discusses the importance of assessing musculoskeletal disease in older women. Contributing factors; Osteoporosis; Osteoarthritis; Exercise and functional impairment.

Published
2003
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19. Lower Gastrointestinal Bleeding

Author

Amin SK and Antunes C

Abstract

Gastrointestinal (GI) bleeding is a term used for any bleeding that occurs within the GI tract from mouth to anus. GI bleeding can be categorized into upper and lower in origin. The ligament of Treitz is commonly used as the point to differentiate the two. Bleeds proximal to the ligament are upper GI bleeds, and distal bleeds are lower GI bleeds. Categorization into one of the two groups is important as it directs the evaluation and management of the patient., (Copyright © 2022, StatPearls Publishing LLC.)

Published
2022

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