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Endogenous opioids serve as modulators of neuroendocrine and immune system processes, the investigation of which calls for high-specificity radioimmunoassays (RIAs). This study focuses on the development and use of a specific radioimmunoassay for the opioid peptide Met5-Enkephalin-Arg6-Phe7 (MEAP), the C-terminus part of proenkephalin A. Antibodies were raised in four rabbits and investigated in terms of titre, avidity and specificity, followed by finding ideal conditions for these antibodies in RIA. MEAP concentrations were determined in crude extracts of rat hypothalamus, dorsal root ganglia, adrenals and ankle using this standardized assay after an oxidizing process. At reverse-phase high-pressure liquid chromatography (HPLC), the position of immunoreactive material from rat hypothalamus eluted as two peaks out of which one was compatible with that of synthetic MEAP. All rabbits exhibited individual differences in relative immune response and time of its onset. The avidity constant was 10 times higher on a molar basis for ab 4108 compared with ab 4182. There was no cross-reactivity for ab 4182 to related peptides, such as enkephalins and dynorphin B, and negligible background values for ab 4108. The relative levels ofimmunoreactive MEAP from the CNS versus peripheral tissues contrasted in accordance with current knowledge. It is suggested that reports with RIA results should include characterization of antibodies, extraction procedures, standard curves and compositions of buffers. Furthermore, the results should preferably be expressed in relation to total protein content. [ABSTRACT FROM AUTHOR]

Investigates the long-term effects of weight change on medication for diabetes and cardiovascular disease in severely obese subjects. Changes in the use and costs of medication in relation to treatment and weight change; Results of the Swedish Obese Subjects (SOS) intervention study.

To examine how soil organisms affect soil organic nitrogen (N) storage and mineralisation, we theoretically derive the soil substrate nitrogen to carbon ratio and total soil N storage for both donor-controlled (DC) and Lotka-Volterra (LV) soil systems with three trophic levels: substrate, microbes and grazers. We show that substrate N:C ratioand soil N storage are functions of the structure of the soil food web, of the properties of the organisms in the soil community, and of the properties of litter input from above ground. The carbon production-to-assimilation efficiency of soil organisms is the most importantproperty determining how the substrate N: C ratio and soil N storageare affected by changes in the soil community. The qualitative effects of grazers depend on whether they have an efficiency larger or smaller than some critical efficiencies. In this paper, we derive these critical efficiencies mathematically. Within the normal range of efficiencies, substrate N:C ratio and soil N storage will usually decrease, and the nitrogen mineralization will increase, with addition of grazers, or as a result of a succession of species from a lower to a higher level of metabolic activity. Numerical analyses show that an increase in soil temperature has a pronounced negative effect on soil N storage through the effects on the metabolic activity of organisms atall trophic levels. N dilution in litter, because of increased CO2 levels in the atmosphere, can lead to a moderate increase in soil N storage. [ABSTRACT FROM AUTHOR]

Presents a correction to the article "Repeated Massage-Like Stimulation Induces Long-Term Effects on Nociception — Contribution of Oxytocinergic Mechanisms," by I. Lund, Y. Ge, L. C. Yu, K. Uvnas-Moberg, J. Wang, C. Yu, M. Kurosawa, G. Agren, A. Rosen, M. Lekman and T. Lundeberg, published in the "European Journal of Neuroscience."

Background: The main aim of this experimental study was to investigate the early effects of intra-abdominal hypertension (IAH) on intra-abdominal metabolism and intestinal mucosal blood flow to evaluate whether metabolites can serve as markers for organ dysfunction during IAH., Methods: A swine model was used, and the animals were anesthetized and ventilated. Fifteen animals were subjected to IAH of 30 mm Hg for 4 hr by carbon dioxide insufflation. Seven animals served as controls. Hemodynamic data, arterial blood samples, and urine output were analyzed. Intraluminal laser Doppler flowmetry measured intestinal mucosal blood flow. Glucose, glycerol, lactate, and pyruvate concentrations and lactate-to-pyruvate (l/p) ratio were measured intraperitoneally and intramurally in the small intestine and rectum using microdialysis., Results: IAH lowered the abdominal perfusion pressure by 12-18 mm Hg, reduced the intestinal mucosal blood flow by 45-63%, and decreased urine output by 50-80%. In the intervention group, glycerol concentrations increased at all locations, pyruvate concentrations decreased, and the l/p ratio increased intraperitoneally and intramurally in the small intestine. Control animals remained metabolically stable. Glucose and lactate concentrations were only slightly affected or unchanged in both the groups., Conclusions: IAH reduces intestinal blood flow and urinary output and causes early metabolic changes, indicating a discrete shift toward anaerobic metabolism. Intraperitoneal microdialysis may be useful in the early detection of impaired organ dysfunction with metabolic consequences in IAH and abdominal compartment syndrome., (Copyright © 2015 Elsevier Inc. All rights reserved.)

Objective: To identify risk factors for serious and specific early complications of laparoscopic gastric bypass surgery using a large national cohort of patients., Background: Bariatric procedures are among the most common surgical procedures today. There is, however, still a need to identify preoperative and intraoperative risk factors for serious complications., Methods: From the Scandinavian Obesity Surgery Registry database, we identified 26,173 patients undergoing primary laparoscopic gastric bypass operation for morbid obesity between May 1, 2007, and September 30, 2012. Follow-up on day 30 was 95.7%. Preoperative data and data from the operation were analyzed against serious postoperative complications and specific complications., Results: The overall risk of serious postoperative complications was 3.4%. Age (adjusted P = 0.028), other additional operation [odds ratio (OR) = 1.50; confidence interval (CI): 1.04-2.18], intraoperative adverse event (OR = 2.63; 1.89-3.66), and conversion to open surgery (OR = 4.12; CI: 2.47-6.89) were all risk factors for serious postoperative complications. Annual hospital volume affected the rate of serious postoperative complications. If the hospital was in a learning curve at the time of the operation, the risk for serious postoperative complications was higher (OR = 1.45; CI: 1.22-1.71). The 90-day mortality rate was 0.04%., Conclusions: Intraoperative adverse events and conversion to open surgery are the strongest risk factors for serious complications after laparoscopic gastric bypass surgery. Annual operative volume and total institutional experience are important for the outcome. Patient related factors, in particular age, also increased the risk but to a lesser extent.