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The formation mechanism of acidic wastewater from abandoned mines was reported. The re- search status of the properties of acidic wastewater in different mine types, and analyzes the impact of acidic wastewater from abandoned mines on the local ecological environment, biological growth, and human health. Based on the main technical methods for the treatment of acid wastewater from abandoned mines, the main classification, technical content, scope of application and economy of plugging technology and acid wastewater treatment technology were introduced respectively. It was suggested that the application of acid wastewater treatment technology from abandoned mines should comprehensively consider various factors, and combine the treatment goals to reasonably select the treatment technology suitable for local acid wastewater from abandoned mines. [ABSTRACT FROM AUTHOR]

[Objective] To select oat varieties with high aphid resistance, strong production performance and excellent comprehensive performance, to provide theoretical references for the healthy development of the oat industry in Inner Mongolia. [Methods] In Shaerqin farming-pastoral test site of Institute of Grassland Research of Chinese Academy of Agricultural Sciences in Tumed Left Banner, Hohhot, taking 17 oat varieties as test materials, the aphid resistance was identified by aphid ratio method. The aphid resistance grade of each variety was determined. And the agronomic traits and yield were used as indicators to compare the differences in production performance of the varieties. Bivariate correlation analysis and systematic cluster analysis were performed using agronomic parameters, yield, and aphid ratio as indicators. [Results] The aphid resistance of the test oat varieties was categorized into 6 grades. Baiyan No.7, Mengyan No.3, Qinghai 444, and Qingyin No.2 had high resistance. Qinghaitian oats and Qingyan No.1 showed medium resistance. Mengsiyan No.2, Lingxiu, Beile, Taiyangshen, and Yellow Oats had low resistance. Bayan No.1 and Bayan No.7 were low susceptible. Bayan No.5 was medium susceptible. Aiwo126, Yanwang and Furuizhi were highly susceptible. The plant height of Baiyan No.7 and Taiyangshen reached over 1.18 m, which was significantly higher than that of Bayan No.7, Lingxiu, Yanwang, Qingyin No.2, Aiwo 126, Furuizhi (P<0.05). Beile had the highest fresh grass yield (4.34 kg/m2), which was significantly higher (P<0.05) than other varieties except Bayan No.5 and Aiwo 126. Taiyangshen had the highest hay yield (1.69 kg/m2), which was significantly higher (P<0.05)than that of Bayan No.7 and Qingyin No.2. There was no significant difference (P>0.05)in the fresh-to-dry ratio of the 17 oat varieties. Aiwo 126 had the highest (2.98) fresh-to-dry ratio, while Qinghai 444 had the lowest (1.86). The results of cluster analysis showed that the aphid ratio, plant height and yield performance of different oat varieties varied. The comprehensive performance of class I oat varieties (Qinghaitian oats, Mengyan No.3, Qinghai 444, Baiyan No.7, Lingxiu, Qingyan No.1, Yellow Oats, Taiyangshen, Bayan No.1, Bayan No.5, Bayan No.7, and Mengsiyan No.2) was stronger. [Conclusion] Qinghaitian oats, Mengyan No.3, Qinghai 444, Baiyan No.7, Lingxiu, Qingyan No.1, Yellow Oats, Taiyangshen, Bayan No.1, Bayan No.5, Bayan No.7 and Mengsiyan No.2 had strong aphid resistance, good production performance, and excellent comprehensive performance.They were suitable for planting in Tumed Left Banner in Inner Mongolia. [ABSTRACT FROM AUTHOR]

随着社会的发展和人口老龄化进程的加速, 慢性病已成为威胁人类健康的主要疾病之一。而糖尿病作为 慢性病中的一种, 对人类健康的危害不容忽视。糖尿病前期患者是指血糖水平高于正常值, 但尚未达到糖尿病诊断标 准的患者, 是糖尿病的高危人群。社区慢性病管理是指通过社区卫生服务机构对慢性病患者进行全面、连续、协调的 管理, 以达到控制病情、延缓病情进展、提高患者生活质量的目的。本文将探讨社区慢性病管理对糖尿病前期患者糖 代谢指标的改善作用。 [ABSTRACT FROM AUTHOR]

Aspergillus flavus (A. flavus) is a well-known diffused fungus that contaminates a great number of crops used for human and animal consumption. How to effectively prevent the contamination becomes a significant problem. Camellia (Camellia oleifera Abel.) is one of the four dominant, woody, oil species in the world. Oil from camellia seeds is rich in essential fatty acids and vitamins. This oil can alleviate malnutrition by balancing human nutrition to benefit human health, and it conforms to the consumption trends of modern edible oils, along with the utilization of its by-products is getting more and more attention. In recent years, the prevention and treatment of A. flavus and AFTs by plant sources and active microbial substances have attracted more attention. Our previous study shows that camellia seed cake is not susceptible to A. flavus. Based on the result, we propose that there may be a certain natural antagonistic substances that can inhibit the growth of A. flavus and the production of AFTs in camellia seeds. This study was designed to evaluate the antifungal effect of the active substance in camellia seed cake on the growth of A. flavus and toxigenicity through in-vitro and in-vivo antifungal tests, to make a determination of antimicrobial substances contained in the camellia seed cake. Camellia seed cake was extracted by eighty percent methanol (v/v), the filtrate was extracted with ethyl acetate and saturated n-butanol. Among the extracts, the n-butanol phase exhibited apparent inhibition activity on the growth and aflatoxin production of A. flavus. The concentration of 100 mg/mL worked best, because the inhibitory zone diameter was 22.00 mm, the mycelial dry weight was 42.88% less than the control, both of the two indicators were significantly better than other treatments (P<0.01), and no AFTs were detected. Whereas the aqueous phase and ethyl acetate phase exhibited weak antifungal activity and no activity, respectively. In addition, the n-butanol phase inhibited the production of aflatoxin B1 (AFB1) of A. flavus effectively, AFB1 was not detected in all concentration treatments. The main chemical component of n-butanol extract phase of eighty percent methanol extract in camellia seed cake was tea saponin, the research showed that tea saponin had no inhibitory effect on A. flavus. Therefore, it was speculated that the other active component (s) in the n-butanol extract phase had inhibitory effect on A. flavus. n-Butanol extract phase was separated and purified by means of AB-8 macroporous absorbent column chromatography, among the macroporous resin fraction of 0%, 25%, 50%, 75%, and 100% ethanol (v/v), the 75% ethanol fraction showed the highest antifungal activity, the concentration of 100 mg/mL worked best, because the inhibitory zone diameter was 163.7 mm, the mycelial dry weight was 81.28% less than the control, both of the two indices were significantly superior to other treatments (P<0.01), and AFTs were undetected. The 75% ethanol fraction was assayed by ultra-performance liquid chromatographyquadrupole time-of-fight mass spectrometry (UPLC-Triple-TOF/MS). The three compounds were identified which were 1) kaempferol-3-O-[β-D-glucopyranosyl-(1→3)-O-α-L-rhamnopyranosyl-(1→6)-O-β-D-galactopyranoside]; 2) kaemferol-3-O- [2-O-β-D-xylopyranosyl-6-O-α-L-rhamnopyranosyl]-β-D-glucopyranoside; and 3) kaempferol-3-O-[6-trans-p-coumaroyl)- β-D-glucopyranosyl-(1→3)-O-α-L-rhamnopyranosyl-(1→6)-O-β-D-galactopyranoside]. This study provides a reference and a guide for research and development of an antifungal agent for A. flavus, and meanwhile broadens utilization of the by-products of camellia seed. [ABSTRACT FROM AUTHOR]

BACKGROUND: Recent studies have confirmed that the success of stem cell transplantation and the function of transplanted cells are closely related to the in vivo microenvironment. Under normal physiological conditions, the liver is not susceptible to exogenous cells. Therefore, a transplant animal model is usually established to damage the host liver or reduce the immune response of the host liver, creating a good condition for the transplantation and proliferation of exogenous cells. OBJECTIVE: To observe the colonization and distribution of embryonic hepatic stem cells from GFP transgenic mice in a rat model of liposome-coated dichloromethylene diphosphate/retrorsine and 1/3 liver resection (L-CL2MDP/RS/PH). METHODS: GFP transgenic mice at gestational 15 days were taken as donors to separate and cultivate embryonic hepatic stem cells. Fluorescence microscope was used to observe the GFP expression followed by immunocytochemical identification. Thirty Sprague-Dawley rats were randomized into experimental group and control group. Rats in the experimental group were given intraperitoneal injection of retrorsine (30 mg/kg, twice, with an interval of 2 weeks). At 1 preoperative day, these rats were subjected to intravenous infusion of L-CL2MDP (1 mL) and 1/3 liver resection followed by intra-spleen transplantation of embryonic hepatic stem cells from GFP transgenic mice. Rats in the control group were only given injection of normal saline instead of L-CL2MDP and retrorsine, but no liver resection. Frozen liver sections were made in the two groups to observe the distribution of GFP-positive hepatic stem cells under the fluorescence microscope at 3, 15, 30 days after transplantation. Moreover, immunohistochemical staining was used to detect the expression of anti-mouse C-Kit antibody in the rat liver. RESULTS AND CONCLUSION: (1) The hepatic stem cells under microscope were uniform with a cobblestone shape. After 5 days of culture, the cells with increased size presented with the shape of fusiform or polygon, and the green fluorescence was observed in cells under the fluorescence microscope. Passage 3 hepatic stem cells expressed CD34 and AFP. (2) At 3 days after spleen transplantation of embryonic hepatic stem cells, GFP positive cells were scattered in the experimental group under the fluorescence microscope, and the GFP positive expression was still visible in the liver at 30 days after transplantation. (3) At 3, 15, and 30 days after transplantation, C-Kit positive cells were visible in the liver of rats in the experimental group. All these findings reveal that embryonic hepatic stem cells from GFP transgenic mice have been successfully transplanted into the spleen of the L-CL2MDP/RS/PH rat model, and the transplanted cells can survive and proliferate in the rat liver. Subject headings: Liver; Stem Cells; Mice, Transgenic; Tissue Engineering Funding: the National Basic Research Program of China (973 Program), No. 2010CB945502; the Scientific Research Plan Project of Guangdong Province, No. 2015B020226004; the Scientific Research Plan Project of Guangzhou City, No. 201508020262 [ABSTRACT FROM AUTHOR]

This paper combed the technical principle, pretreatment, sample transfer and detection process of quantum cascade laser imaging for analyzing microplastics in soil samples, and summarized and compared the technical application and limitation analysis of physical analysis and chemical analysis (microscope analysis, spectral analysis, mass spectrometry analysis), in eluded the technical process of the main test method, the applicable field, and the advantages and disadvantages. On the basis of the above analysis, the applicability and advancement of the quantum cascade laser imaging in the detection of microplastics in soil samples were summarized・ An important role in the research of microplastics in soil environment and the prevention and control of pollution were played in this method. [ABSTRACT FROM AUTHOR]

BACKGROUND: Retrorsine (RS) is a chemical agent for the long-term inhibition of mature liver cell division and proliferation. OBJECTIVE: To establish a rat model of liver injury by combined use of RS and 1/3 partial hepatectomy, to observe the proliferation of liver cells and oval cells in rats after liver injury, and to discuss the relationship between liver regeneration and mature liver cells and oval cells after liver injury. METHODS: Thirty male Sprague-Dawely rats were randomized into two groups (n=15 per group): RS group and control group. Rats in the RS group were subjected to intraperitoneal injection of RS, 30 mg/kg, twice in total, with 2 weeks in between; and rats in the control group were injected physiological saline instead of RS. Four weeks after the last injection, the 1/3 partial hepatectomy was performed in two groups of rats. Liver pathological and morphological changes as well as cell proliferation were observed, and CK19 and C-kit immunohistochemical detections of the rat liver in the two groups were conducted at different time points after operation. RESULTS AND CONCLUSION: At 20 days after operation, the body mass of the RS group rats was still lower than the baseline, and the liver increase was obviously less than that in the control group; there was cell body swelling shown by hematoxylin-eosin staining, loose cytoplasm, extensive vacuoles degeneration of liver cells, and clustered or scattered oval cells around the portal area of small bile duct and in the hepatic lobule. However, in the control group, the body mass was close to the baseline, liver damage was lighter than that in the RS group, a large number of mature liver cells proliferated under BrdU Immunofluorescence at 20 days after operation; liver oval cells proliferated and distributed in the liver cell line at 14 days after operation, with morphology and immunohistochemical markers consistent with oval cells in the RS group. These findings indicate that the rat model of acute liver injury is successfully established by combining retrorsine with 1/3 partial hepatectomy, liver poisoning and the proliferation of liver stem cells and mature liver cells after poisoning can be seen in the experiment, which firmly confirm that liver cell renewal and regeneration after injury is accredited to the combined action of liver stem cells in liver basin and mature liver cells. [ABSTRACT FROM AUTHOR]

Objective：To investigate the clinicopathological features, diagnosis, treatment and prognosis of female genital angiomyofibroblastoma (AMF). Methods：Retrospective analysis of the clinical data of 10 female genital AMF patients treated during January 2012 to October 2017, combined with related literatures, summarized their clinical and pathological features, diagnosis, treatment and prognosis. Results：The average onset age was (49.90±7.62) years old in 10 patients with AMF. The patients had no obvious clinical symptoms, and no specificity in imaging examination. Under the microscope, the cell-rich area and cell-sparse area were alternately distributed, the tumor tissue was composed of significantly small lumen, thin tube wall and dilated blood vessels which were surrounded by eosinophilic circular or fusiform tumor cells, and the tumor matrix was loose, edema and mucus like, and nuclear fission was rare. All the 10 patients underwent partial complete resection. 8 cases were followed up and the median follow-up time was 37 months. No recurrence occurred. Conclusions：There is no specificity in the clinical manifestation and imaging of AMF which occurs in female genital organs. The diagnosis and differential diagnosis mainly depends on the pathological features and immunohistochemistry. The surgical treatment is the main treatment and the prognosis is good. [ABSTRACT FROM AUTHOR]

Objective: To investigate the risk factors of malignant ventricular arrhythmias in elderly patients with ST segment elevation myocardial infarction (STEMI). Methods: 150 elderly patients with acute STEMI were analyzed retrospectively. Based on the presence or absence of malignant ventricular arrhythmia (MVA), the patients were divided into the control group (non-MVA) and observation group (MVA), the clinical data and indicators were compared between two groups. Results: Gender, age, smoking history, history of hypertension, diabetes mellitus, history of angina, blood glucose, glycosylated hemoglobin (HbA1c), serum potassium, creatinine (Cr), BUN, triglyceride (TG), total cholesterol (TC), high-density lipoprotein (HDL), low density lipoprotein (LDL) and lesion type of MVA had no relationship with the occurrence of MVA in elderly patients with STEMI, while Killip classification, Cr, and LDL were correlated with the occurrence of MVA, and Killip class, creatinine levels were independent risk factors for the occurrence of MVA. Conclusions: Killip classification and Cr levels were independent risk factors of the occurrence of MVA in elderly patients with STEMI. [ABSTRACT FROM AUTHOR]

目前,卵巢透明细胞癌(OCCC)仍按照卵巢上皮癌(EOC)治疗方法,一线治疗采用经典的TC 方案,通过此案例治疗效果体会到多线的含铂化疗方案并未使患者受益,体现了卵巢透明细胞癌对常规铂类化 疗敏感度底的特点,化疗效果有限,而免疫治疗联合靶向药物治疗有效,使患者生存受益。传统的EOC治疗方 案受到了挑战。而近10年来开展的治疗项目研究也主要聚焦于靶向药、免疫制剂等生物治疗,提示了免疫及 靶向治疗可能为今后治疗趋势。 [ABSTRACT FROM AUTHOR]

Hongjiannao Lake plays an important role in water conservation, sand control, biodiversity maintaining, etc, and has been the biggest breeding habitat of Larus relictus in the world. Hence the research about the changing of its water area is significant. Based on 6 phases of remote sensing data from1989 of Hongjiannao Lake and the existing data, the article studied the evolution of its water area, discussed the reasons, and explored its destiny. The analyses showed that the evolution of Hongjiannao lake surface area can be divided into three stages, which are expand stage from the 1920s to 1950s, stable stage from 1960s to 1990s, and shrinking stage since the late 1990s. Climatic change and human activities play an important role in this process. Enlargement of the lake resulted from rapid concentration of water resources in a short time due to large precipitation and the development of drainage channels in agriculture. The supply of lake is approximate same with its consumption in the stable stage. In shrinking stage, the clean water runoff generation mechanism of Hongjiannao basin had been damaged by human activities, such as dam construction, groundwater withdrawal by digging and mineral resource exploration, which can greatly decrease the input amout of water. Based on the current evolution trend, it is inevitable to dry up of Hongjiannao Lake without any protective measures. [ABSTRACT FROM AUTHOR]

Objective To explore cardiac function and myocardial collagen type I in diet-induced insulin-resistant rats and the effect of telmisartan on cardiac diastolic function in diet-induced insulin-resistant rats. Methods We randomized 27 Wistar rats into control group (n=9), high-fat group (n=9), and telmisartan treatment group (n=9). At the end of the study, left ventricular end diastolic pressure (LVEDP) and left ventricular systolic pressure (LVSP) of the rats and ±dp/dt were detected by carotid artery intubation. Masson cardiac staining was used to observe cardiac fibrosis, and collagen volume fraction (CVF) was measured. ELISA method was used to detect the concentration of plasma PICP and ICTP. Results Compared with the control group, in high-fat group LVEDP was significantly higher and -dP/dtmax decreased significantly (P<0.01); the plasma PICP level and the ratio of PICP/ICTP were significantly increased (P<0.01), cardiac collagen volume fraction was significantly higher (P<0.01). After 22 weeks' telmisartan intervention, compared with the high-fat group, LVEDP and LVSP were significantly decreased (P<0.01), but -dP/dtmax significantly increased (P<0.05). The level of the plasma PICP and PICP/ICTP were significantly decreased (P<0.05); left ventricular myocardial tissue collagen volume fraction content was decreased (P<0.01). The correlation analysis showed that cardiac collagen volume fraction in insulin-resistant group was positively correlated with insulin resistance index but negatively correlated with -dp/dtmax (P<0.01). Conclusion Insulin resistance promoted the synthesis of myocardial type I collagen, leading to increased myocardial collagen deposition and decreased cardiac diastolic function. Telmisartan may improve diastolic function partly by improving insulin resistance and reducing the deposition of myocardial collagen type I. [ABSTRACT FROM AUTHOR]