1. Measurement of 5-hydroxytryptamine release in the rat medial vestibular nucleus using in vivo microdialysis.
- Author
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Inoue S, Kita T, Yamanaka T, Ogawa Y, Nakashima T, and Hosoi H
- Subjects
- 8-Hydroxy-2-(di-n-propylamino)tetralin pharmacology, Animals, Calcium Signaling drug effects, Calcium Signaling physiology, Clomipramine pharmacology, Extracellular Space drug effects, Male, Microdialysis, Potassium Chloride pharmacology, Presynaptic Terminals drug effects, Rats, Rats, Wistar, Serotonin Receptor Agonists pharmacology, Selective Serotonin Reuptake Inhibitors pharmacology, Synaptic Transmission drug effects, Up-Regulation drug effects, Up-Regulation physiology, Vestibular Nuclei drug effects, Extracellular Space metabolism, Presynaptic Terminals metabolism, Serotonin metabolism, Synaptic Transmission physiology, Vestibular Nuclei metabolism
- Abstract
The function of the 5-hydroxytryptamine (5-HT) system in the medial vestibular nucleus (MVN) was evaluated using KCl-evoked 5-HT release assessed by in vivo microdialysis. The release of 5-HT in the MVN was increased by the local application of KCl (100 mM) in a calcium-dependent manner. Likewise, perfusion of a 5-HT reuptake blocking agent, clomipramine (1 microM), increased extracellular levels of 5-HT in the MVN. Finally, pretreatment with the selective 5-HT(1A) agonist, 8-hydroxy-2-(di-N-propylamino) tetralin (1 mg/kg, intraperitoneally), attenuated the KCl-evoked 5-HT release. These findings suggest that 5-HT stored in vesicles is released from nerve terminals in the MVN. In addition, its regulation may be mediated by control of somatodendritic 5-HT(1A) receptor activation.
- Published
- 2002
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