2,662 results on '"Yang, Yu"'
Search Results
2. Quantum blockchain based on asymmetric quantum encryption and a stake vote consensus algorithm
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Wusheng Wang, Yang Yu, and Lingjie Du
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Medicine ,Science - Abstract
Abstract As emerging next-generation information technologies, blockchains have unique advantages in information transparency and transaction security. They have attracted great attentions in social and financial fields. However, the rapid development of quantum computation and the impending realization of quantum supremacy have had significant impacts on the advantages of traditional blockchain based on traditional cryptography. Here, we propose a blockchain algorithm based on asymmetric quantum encryption and a stake vote consensus algorithm. The algorithm combines a consensus algorithm based on the delegated proof of stake with node behaviour and Borda count (DPoSB) and quantum digital signature technology based on quantum state computational distinguishability with a fully flipped permutation ( $${\text{QSC}}{\text{D}}_{\text{ff}}$$ QSCD ff ) problem. DPoSB is used to generate blocks by voting, while the quantum signature applies quantum one-way functions to guarantee the security of transactions. The analysis shows that this combination offers better protection than other existing quantum-resistant blockchains. The combination can effectively resist the threat of quantum computation on blockchain technology and provide a new platform to ensure the security of blockchain.
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- 2022
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3. Selinexor plus low-dose dexamethasone in Chinese patients with relapsed/refractory multiple myeloma previously treated with an immunomodulatory agent and a proteasome inhibitor (MARCH): a phase II, single-arm study
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Lugui Qiu, Zhongjun Xia, Chengcheng Fu, Wenming Chen, Chunkang Chang, Baijun Fang, Gang An, Yongqiang Wei, Zhen Cai, Sujun Gao, Jianyu Weng, Lijuan Chen, Hongmei Jing, Fei Li, Zhuogang Liu, Xiequn Chen, Jing Liu, Aihua Wang, Yang Yu, Wenxi Xiang, Kevin Lynch, Zhinuan Yu, and Weijun Fu
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Multiple myeloma ,Relapsed and refractory ,Selinexor ,Chinese patients ,Medicine - Abstract
Abstract Background Selinexor 80 mg combined with low-dose dexamethasone (Sd) demonstrated significant clinical benefit in patients with relapsed/refractory multiple myeloma (RRMM) who had disease refractory to a proteasome inhibitor (PI), an immunomodulator (IMiD), and an anti-CD38 monoclonal antibody based on a global phase II STORM study. The present study, MARCH, addresses China regulatory needs to further validate the data from STORM in Chinese patients with RRMM. Methods The MARCH study was conducted at 17 sites in China, where eligible Chinese RRMM patients who had disease refractory to PI and IMiD were enrolled. Selinexor 80 mg combined with dexamethasone 20 mg was administered orally on day 1 and day 3 of each week in 4-week cycles. The primary endpoint was the overall response rate (ORR) per an independent review committee, with the null hypothesis of ≤15%. Patients who received at least 1 dose of study treatment were included in the safety population. The pharmacokinetic (PK) profile was characterized by parameter and ethnicity sensitivity analyses. Results A total of 82 patients with RRMM were enrolled in the study, with a median age of 60 years. Of the 82 patients, 55 patients (67.1%) had high-risk cytogenetic abnormalities, defined as one or more of del 17p13, t(4;14), t(14;16), or 1q amplification identified by fluorescence in situ hybridization (FISH); 18 patients (22.0%) had abnormal renal function. Enrolled patients were heavily pre-treated with a median prior regimen number of 5. All 82 patients (100%) were refractory to both PI and IMiD, including 20 patients (24.4%) categorized as triple-class refractory population (refractory to PI, IMiD, and daratumumab). Ten patients (12.2%) had undergone CAR-T therapy. ORR was 29.3% (95% CI 19.7, 40.4) with a median DOR of 4.7 months. The median PFS and OS were 3.7 and 13.2 months, respectively. ORR was 25.0% (95% CI 8.7, 49.1) in the triple-class refractory population. Efficacy was consistent across various subgroups. The most frequent grade 3/4 adverse events (AEs) included anemia (57.3%), thrombocytopenia (51.2%), lymphopenia (42.7%), neutropenia (40.2%), hyponatremia (29.3%), and lung infection (26.8%). Serious AEs were reported in 54.9% of patients. No significant drug accumulation was shown following multiple administrations. No human PK ethnicity difference was identified between Chinese and western patients. Conclusions With an encouraging ORR, the MARCH study has demonstrated that selinexor combined with low-dose dexamethasone (Sd) delivers meaningful clinical benefit to Chinese patients with RRMM, including triple-class refractory patients. AEs were expected and manageable with supportive care and dose modification. Trial registration ClinicalTrials.gov, NCT03944057 (May 09, 2019); Chinadrugtrials.org.cn , CTR20190858 (June 05, 2019)
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- 2022
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4. Propensity score matching analysis to evaluate efficacy of polyethylene oxide adhesive on preventing delayed bleeding after gastric endoscopic submucosal dissection
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Yang Yu, Tong Hu, Xiaoyi Kuai, Xiaoyu Liu, Rui Li, and Chunli Zhou
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Medicine ,Science - Abstract
Abstract Regardless of technical advancements, delayed bleeding is still a common adverse event after gastric endoscopic submucosal dissection (ESD), often occurring in the early postoperative phase. This study aimed to evaluate the efficacy of a newly designed polyethylene oxide (PEO) adhesive for preventing delayed gastric bleeding. Patients who underwent gastric ESD between December 2017 and December 2020 at three Chinese institutions were retrospectively reviewed. Patients receiving PEO application on gastric post-ESD ulcers were included in the PEO group, and patients without this procedure were included in the control group. To minimize potential bias, propensity score matching was performed, and sex, age, lesion size, lesion morphology, ulceration, localization, procedure time, frequency of major intraoperative bleeding, resected specimen size, lesion histopathology, submucosal invasion and the taking of antithrombotic drugs were included as matching factors. The incidence of delayed bleeding and time to bleeding were compared between both groups. After propensity score matching, 270 patients (135 per group) were included in the analysis. The delayed bleeding rate in the PEO group was significantly lower than that in the control group (1.5%, 2/135 vs. 8.9%, 12/135, P = 0.006). The median time (range) to bleeding was 4.5 (4–5) days in the PEO group and 2 (1–15) days in the control group, with no significant difference (P = 0. 198). PEO demonstrated a significant effect in reducing the rate of delayed bleeding. Further study is warranted to confirm the efficacy of PEO for bleeding that occurs in the early phase after gastric ESD.
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- 2022
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5. The relationship between a fish-rich diet and poststroke cognitive impairment
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Jia-Rui Li, MD, Yang Yu, MD, Fan-Xia Meng, MD, Jie Yu, MD, Ben-Yan Luo, MD, and Jian Gao, MD
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Medicine - Abstract
Abstract. Whether a fish-rich diet is positively associated with cognitive function after stroke remains unclear; thus, the present study investigated the relationship between them. The present study was part of a prospective multicenter study, in which 920 individuals (609 males, mean age, 62.78 ± 11.79 years) were included from November 2013 to December 2015. The cognitive function of the patients was evaluated, and the diagnosis of poststroke cognitive impairment (PSCI) was made during their stay in the hospital. A subgroup of 439 patients from a single center was followed up for 4 to 6 years and was reassessed for cognitive function. According to the diagnostic criteria, the PSCI prevalence was lower in the fish-rich diet group (P
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- 2022
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6. Sintilimab combined with bevacizumab in relapsed/persistent ovarian clear cell carcinoma (INOVA): an investigator-initiated, multicentre clinical trial—a study protocol of clinical trial
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Yang Yu, Wei Zhang, Guiling Li, Ming Li, Xingyu Liu, Jie Jiang, Yingjun Zhao, Ruyuan Li, Chunyan Song, Jiahao Liu, Xiaofei Jiao, Shaoqing Zeng, Jianhua Chi, Guanchen Ma, Yabing Huo, Zikun Peng, and Qing-Lei Gao
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Medicine - Published
- 2022
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7. Analysis on carbon emissions efficiency differences and optimization evolution of China's industrial system: An input-output analysis.
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Shuang Wang, Yang Yu, Tangyang Jiang, and Jun Nie
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Medicine ,Science - Abstract
The excessive carbon emissions not only intensify the global climate change, but also seriously restrict the sustainable development of social economy. However, improving industrial carbon emissions efficiency is the most directly effective way to reduce emissions. Therefore, accurate measurement and analysis of carbon emissions efficiency and evolution characteristics of China's industrial system is the basis for China to improve carbon emissions efficiency. Based on this, we adopted energy consumption method and input-output method to calculate and analyze the industrial carbon emissions efficiency and evolution characteristics of China from 2002 to 2015. The results show that (1) If carbon emissions from cement production are ignored and only energy-related carbon emissions are considered, the calculation results of carbon emissions efficiency of heavy industry will be overestimated about 30%. (2) Compared with 2002, China's industrial carbon emissions efficiency increased by about twice in 2015. Specifically, the optimization of carbon emissions efficiency in agriculture, construction, light industry and service industry is very obvious, which has increased by 5.65, 5.00, 4.69 and 4.68 times respectively; The optimization of carbon emissions efficiency in power sector, heavy industry, transportation and chemical industry was obvious, which increased by 2.55, 2.01, 1.86 and 1.47 times respectively; The carbon emissions efficiency of the fossil energy sector has decreased by 0.36 times. (3) The carbon emissions efficiency of various industries has significant differences. Among them, agriculture, service industry, light industry and construction industry are generally industries with higher carbon emissions efficiency, while power sector, fossil energy sector, transportation industry, chemical industry and heavy industry are generally industries with lower carbon emissions efficiency, especially power and fossil energy sectors. Based on these, this paper provides policy implications and scientific evidence for accurately improving carbon emissions efficiency from the perspective of carbon emissions efficiency.
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- 2022
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8. SNP rs12982687 affects binding capacity of lncRNA UCA1 with miR-873-5p: involvement in smoking-triggered colorectal cancer progression
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Yang Fu, Yizheng Zhang, Jinyuan Cui, Ge Yang, Sanfei Peng, Wunan Mi, Xiangya Yin, Yang Yu, Jianwu Jiang, Qi Liu, Yiyu Qin, and Wen Xu
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CRC ,lncRNA UCA1 ,Single nucleotide polymorphism ,Gene-environment interaction ,miR-873-5p ,Nicotine ,Medicine ,Cytology ,QH573-671 - Abstract
Abstract Background This investigation was arranged to elucidate whether single nucleotide polymorphisms (SNPs) of lncRNA UCA1 was implicated in elevating colorectal cancer (CRC) risk by interacting with environmental exposures. Methods LncRNASNP database was firstly adopted to predict SNPs that possibly affected binding of UCA1 with miRNAs and then the interactive effect of SNPs and environmental exposure on CRC risk was evaluated by recurring to type 2 gene-environment interactions (GEI) model. Besides, MTT assay, colony formation assay, transwell assay and wound healing assay were performed to assess the activity of CRC cell lines which carried distinct genotypes of specific SNPs. The impact of nicotine on activity of CRC cells was also appraised. Results SNP rs12982687 of UCA1 intervened in the binding capacity of UCA1 with several miRNAs, especially miR-873-5p. MiRNAs regulated by UCA1, as predicted by mirPath software, shared genes that were enriched in HIF1 signaling pathway. Moreover, homozygote TT of rs12982687 reduced CRC risk among smokers, and CRC cells that carried rs12982687 (CC) displayed strong migration and invasion. By contrast, miR-873-5p mimic, which reduced UCA1 expression, delayed metastasis of CRC cells (all P
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- 2020
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9. Listeria monocytogenes Associated with Pasteurized Chocolate Milk, Ontario, Canada
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Heather Hanson, Yvonne Whitfield, Christina Lee, Tina Badiani, Carolyn Minielly, Jillian Fenik, Tony Makrostergios, Christine Kopko, Anna Majury, Elizabeth Hillyer, Lisa Fortuna, Anne Maki, Allana Murphy, Marina Lombos, Sandra Zittermann, Yang Yu, Kristin Hill, Adrienne Kong, Davendra Sharma, and Bryna Warshawsky
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Listeria monocytogenes ,bacteria ,listeriosis ,outbreak ,chocolate milk ,pasteurization ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Abstract
In an investigation of a listeriosis outbreak in Ontario, Canada, during November 2015–June 2016, pasteurized chocolate milk was identified as the source. Because listeriosis outbreaks associated with pasteurized milk are rare in North America, these findings highlight that dairy products can be contaminated after pasteurization.
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- 2019
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10. Three-dimensional reconstruction of a whole insect reveals its phloem sap-sucking mechanism at nano-resolution
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Xin-Qiu Wang, Jian-sheng Guo, Dan-Ting Li, Yang Yu, Jaco Hagoort, Bernard Moussian, and Chuan-Xi Zhang
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nilaparvata lugens ,3D reconstruction ,whole insect body ,phloem sap-sucking mechanism ,serial block face scanning electron microscopy (sbf-sem) ,Medicine ,Science ,Biology (General) ,QH301-705.5 - Abstract
Using serial block-face scanning electron microscopy, we report on the internal 3D structures of the brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae) at nanometer resolution for the first time. Within the reconstructed organs and tissues, we found many novel and fascinating internal structures in the planthopper such as naturally occurring three four-way rings connecting adjacent spiracles to facilitate efficient gas exchange, and fungal endosymbionts in a single huge insect cell occupying 22% of the abdomen volume to enable the insect to live on plant sap. To understand the muscle and stylet movement during phloem sap-sucking, the cephalic skeleton and muscles were reconstructed in feeding nymphs. The results revealed an unexpected contraction of the protractors of the stylets and suggested a novel feeding model for the phloem sap-sucking.
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- 2021
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11. Inflection-Point Nutrition Support Determined by Oral Mucosal Apoptosis Rate Is a Novel Assessment Strategy for Personalized Nutrition: A Prospective Cohort Study
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Chun Gao, Zike Li, Sheng Zhang, Dengyi Cao, Yang Yu, Yujie Zhang, Hao Chen, Dehua Fu, and Jianping Gong
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inflection-point nutrition ,gastric cancer ,apoptosis ,nutritional support ,postoperative recovery ,Medicine - Abstract
Background: Energy intake and nutritional status influences a patient’s recovery from major abdominal surgery. The aim of this study is to explore and validate the clinical feasibility of an inflection-point nutrition strategy for personalized nutrition in gastric cancer patients after surgery. Methods: We conducted a prospective cohort study from a single tertiary referral hospital. Patients diagnosed with gastric cancer who met the inclusion criteria were included in this study. We collected the demographic and clinic pathological characteristics of included patients. Patients were divided into a formular nutrition (FN) and inflection-point nutrition (IPN) group. We monitored the perioperative dynamics of the oral mucosal epithelia cell apoptosis rate. Predictive factors for inflection phenomenon were investigated in univariate and multivariate analysis. Results: A total of 53 gastric cancer patients were included. A total of 30 (56.6%) patients showed the inflection phenomenon, with 9 (34.6%) patients in the FN group and 21 (77.8%) patients in the IPN group, respectively. We found that patients with the inflection phenomenon had a shorter duration of hospital stay compared to patients without the inflection phenomenon (p = 0.04). In multivariate analysis, independent predictive factors for inflection phenomenon were age (p = 0.015), operation time ≤ 300 min (p = 0.012), and average energy intake ≥ 25 Kcal/kg/day (p = 0.038). Conclusions: Our findings for the first time revealed that the oral epithelial cell apoptosis rate can promptly reflect the patients’ perioperative nutrition needs. Meanwhile, we developing a novel and feasible nutrition therapy guided by the oral epithelial cell apoptosis rate is novel in gastric cancer patients that have undergone laparoscopic gastrectomy.
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- 2022
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12. Three paraneoplastic signs in the same patient with gastric adenocarcinoma
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Yang, Yu-hua, Zhang, Ru-zhi, Kang, Ding-hua, and Zhu, Wen-yuan
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Medicine - Abstract
This is the report of a 76-year-old male with typical lesions of acanthosis nigricans maligna (ANM), florid cutaneous papillomatosis (FCP), and tripe palms (TP) for 2 years. He did not have any gastrointestinal complaints. Pathologic findings of skin supported the diagnosis of ANM. Because gastric adenocarcinoma is the most common neoplasm associated with these paraneoplastic dermatoses, further tests were carried out. Endoscopic examination was performed and an adenocarcinoma of the esophagogastric junction was confirmed. Meanwhile, multiple small polyps in the middle and the lower thirds of the esophagus were observed. The patient was referred for further evaluation and subsequent surgical resection of the tumor.Acanthosis nigricans (AN) is a hyperkeratotic mucocutaneous eruption of heterogenous etiology, which is characterized by hyperpigmentation, velvety cutaneous thickening, intensified skin markings, and development of verrucous excrescences typically involving the intertriginous areas. AN is classified into benign and malignant forms on the basis of clinical associations. Malignant acanthosis nigricans (MAN) tends to be extensive and involves mucosal surfaces, mostly in elderly people. Florid cutaneous papillomatosis (FCP), also known as the Schwartz-Burgess syndrome, is characterized by the rapid appearance of multiple verrucous lesions that are clinically indistinguishable from common warts [1]. Tripe palms (TP) is characterized by diffuse, yellowish palmar hyperkeratosis, with enhancement of the epidermal ridges on the hands (dermatoglyphics), resembling intestinal villosities [1]. The association of these three paraneoplastic dermatoses (FCP, ANM and TP) in the same patient has been reported. Herein, we report an elderly male with three paraneoplastic dermatoses for two years. On the initial presentation, he did not report any systemic complaints; diagnostic tests confirmed the presence of a gastric adenocarcinoma.
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- 2013
13. Transcriptomic and proteomic profiles of II YOU 838 (Oryza sativa) provide insights into heat stress tolerance in hybrid rice
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Yan Wang, Yang Yu, Min Huang, Peng Gao, Hao Chen, Mianxue Liu, Qian Chen, Zhirong Yang, and Qun Sun
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Hybrid rice ,Flag leaf ,Heat stress tolerance ,Transcriptome ,Proteome ,Co-regulatory network ,Medicine ,Biology (General) ,QH301-705.5 - Abstract
Heat stress is an increasing threat to rice production worldwide. To investigate the mechanisms of heat tolerance in hybrid rice and their contributions to rice heterosis, we compared the transcriptome of the hybrid rice II YOU 838 (II8) with the transcriptomes of its parents Fu Hui 838 (F8) and II-32A (II3) after heat stress at 42 °C for 0 h, 24 h, 72 h and 120 h. We also performed a proteomic analysis in II8 after heat stress at 42 °C for 24 h. The transcriptome data revealed time-dependent gene expression patterns under the heat stress conditions, and the heat stress response of II8 was greatly different from those of its parents. Gene ontology analysis of the differentially expressed genes that were clustered using k-means clustering showed that most of the up-regulated genes were involved in responses to stimuli, cell communication, and metabolic and transcription factor activities, whereas the down-regulated genes were enriched in photosynthesis and signal transduction. Moreover, 35 unique differentially abundant proteins, including a basic helix-loop-helix transcription factor (bHLH96), calmodulin-binding transcription activator, heat shock protein (Hsp70), and chaperonin 60 (CPN60), were detected in the proteomic analysis of II8 under heat stress. The co-regulatory analysis revealed novel genes and pathways involved in heat tolerance, namely, ferredoxin-NADP reductase, peroxidases, mitogen-activated protein kinase kinase kinase, and heat shock factor (HSF)–Hsp network. Members of the Hsp and HSF families had over-dominant expression patterns in the hybrid compared with its parents, to help maintain the higher photosynthesis and antioxidant defense systems in the hybrid. Our study suggests that the complex HSF–Hsp regulatory network contribute to the heat tolerance of the hybrid rice.
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- 2020
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14. Estrogen improves the development of yak (Bos grunniens) oocytes by targeting cumulus expansion and levels of oocyte-secreted factors during in vitro maturation
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Yangyang Pan, Meng Wang, Libin Wang, Qian Zhang, Abdul Rasheed Baloch, Honghong He, Gengquan Xu, Jamila Soomro, Yan Cui, Sijiu Yu, and Yang Yu
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Medicine ,Science - Abstract
The estrogen-signalling pathway is critical for normal follicular development; however, little is known about its importance during in vitro maturation (IVM) in large animals, particularly yaks (Bos grunniens). Through the present study, we aimed to determine the mechanisms underlying estrogen involvement in cumulus expansion and the subsequent development of cumulus-oocyte complexes (COCs). COCs were cultured in the maturation medium supplemented with different concentrations (10−6–10−3 mM) of 17β-estradiol (E2) or its receptor antagonist, fulvestrant, and quantitative reverse-transcription polymerase chain reaction (qRT-PCR) and western blot were performed to determine the expression of cumulus-expansion related factors and oocyte-secreted factors (OSFs). The cumulus expansion of COCs was observed using an inverted microscope, and COCs developmental ability were judged by the evaluation of cleavage and blastulation rates per inseminated oocytes by IVF, and the number of cells in the blastocyst. Cumulus expansion increased with 10−6–10−3 mM E2, but decreased with fulvestrant. HAS2, PTGS2, PTX3 and OSFs expression increased in the 10−6–10−3 mM E2 groups. Significantly higher cleavage and blastocyst rates were observed in the 10−4 mM E2 group than in the fulvestrant and 0 mM E2 groups. Moreover, in the 10−4 mM group, blastocysts at 7 days had higher cell counts than the other groups. In conclusion, the increase in cumulus expansion and subsequent oocyte development after the addition of E2 to IVM medium may have resulted from increased cumulus-expansion-related factor expression and OSF levels.
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- 2020
15. Effect of Subthalamic Nucleus Deep Brain Stimulation (STN-DBS) on balance performance in Parkinson's disease
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Haitao Li, Siquan Liang, Yang Yu, Yue Wang, Yuanyuan Cheng, Hechao Yang, Xiaoguang Tong, and Karsten Witt
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Medicine ,Science - Abstract
Purpose To study the effect of STN-DBS on balance performance of Parkinson's disease. Method 16 idiopathic PD patients treated with bilateral STN-DBS (DBS Group) and 20 PD patients treated with Levodopa (Medicine group) were included in the study. Clinical material including Levodopa Equivalent Daily Dose (LEDD, mg/day), life quality (PDQ-39) were collected. For DBS group and Medicine group, The motor disability (Movement Disorder Society-Sponsored Revision of the Unified Parkinson’s Disease Rating Scale Ⅲ, MDS-UPDRSIII) and balance performance (MDS-UPDRS 3.12, Berg Balance Scale BBS) and the Limits of Stability (LoS) (target acquisition percentage, trunk swing angle standard deviation, time) in state of Med-Off/Med-On at preoperation, postoperation, 6 months postoperation and 12 months postoperation were evaluated. Repeated ANOVA was used to analyze the effect of STN-DBS on balance performance. Result The Clinical material (age, gender, duration, LEDD preoperation, PDQ39), motor disability (Med-on/Med-Off), balance performance (Med-on/Med-Off) and LoS preoperation had no differences in DBS-group and Medical-group (P>0.05). During the follow up, LEDD, PDQ39, Motor disability (MDS-UPDRSIII), balance performance (MDS-UPDRS 3.12, BBS) in Medicine-group had no significant changes in both Med-Off and Med-On. For DBS-group, immediately improvement of motor disability (MDS-UPDRSIII), LoS (target acquisition percentage, trunk swing angle standard deviation, time) and LEDD were observed postoperation (P
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- 2020
16. Overall Blastocyst Quality, Trophectoderm Grade, and Inner Cell Mass Grade Predict Pregnancy Outcome in Euploid Blastocyst Transfer Cycles
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Yan-Yu Zhao, Yang Yu, and Xiao-Wei Zhang
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Blastocyst Inner Cell Mass ,Embryo Transfer ,Fertilization In vitro Genetic Testing ,Trophoblasts ,Medicine - Abstract
Background: Despite recent advances that have improved the pregnancy success rates that can be achieved via in vitro fertilization (IVF) therapy, it is not yet clear which blastocyst morphological parameters best predict the outcomes of single blastocyst transfer. In addition, most of the previous studies did not exclude the effect of embryo aneuploidy on blastocysts transfer. Thus, the present study investigated the predictive value of various parameters on the pregnancy outcomes achieved via the transfer of frozen euploid blastocysts. Methods: The study retrospectively analyzed 914 single euploid blastocyst transfer cycles that were performed at the Peking University Third Hospital Reproductive Medical Center between June 2011 and May 2016. The expansion, trophectoderm (TE), and inner cell mass (ICM) quality of the blastocysts were assessed based on blastocyst parameters, and used to differentiate between “excellent”, “good”, “average”, and “poor”-quality embryos. The relationship between these embryo grades and the achieved pregnancy outcomes was then analyzed via the Chi-square and logistic regression tests. Results: For embryo grades of excellent, good, average and poor, the clinical pregnancy rates were 65.0%, 59.3%, 50.3% and 33.3%, respectively; and the live-birth rates were 50.0%, 49.7%, 42.3% and 25.0%, respectively. Both the clinical pregnancy rate (χ2 = 21.28, P = 0.001) and live-birth rate (χ2 = 13.50, P < 0.001) increased with the overall blastocyst grade. Both rates were significantly higher after the transfer of a blastocyst that exhibited either an A-grade or B-grade TE, and similarly, an A-grade ICM, than after the transfer of a blastocyst that exhibited a C-grade TE and/or ICM. The degree of blastocyst expansion had no apparent effect on the clinical pregnancy or live-birth rate. All odds ratio were adjusted for patient age, body mass index, length (years) of infertility history, and infertility type. Conclusions: A higher overall euploid blastocyst quality is shown to correlate most strongly with optimal pregnancy outcomes. The study thus supports the use of the described TE and ICM morphological grades to augment current embryo selection criteria.
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- 2018
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17. Perioperative Single-Donor Platelet Apheresis and Red Blood Cell Transfusion Impact on 90-Day and Overall Survival in Living Donor Liver Transplantation
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Wei Zheng, Kang-Mei Zhao, Li-Hui Luo, Yang Yu, and Sheng-Mei Zhu
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Apheresis Platelets ,Blood Transfusion ,Living Donor Liver Transplantation ,Outcome ,Red Blood Cell ,Medicine - Abstract
Background: Although many previous studies have confirmed that perioperative blood transfusion is associated with poor outcomes after liver transplantation (LT), few studies described the influence of single-donor platelet apheresis transfusion in living donor LT (LDLT). This study aimed to assess the effect of blood products on outcomes for LDLT recipients, focusing on apheresis platelets. Methods: This retrospective study included 126 recipients who underwent their first adult-to-adult LDLT. Twenty-four variables including consumption of blood products of 126 LDLT recipients were assessed for their link to short-term outcomes and overall survival. Kaplan-Meier survival curve and the log-rank test were used for recipient survival analysis. A multivariate Cox proportional-hazard model and a propensity score analysis were applied to adjust confounders after potential risk factors were identified by a univariate Cox analysis. Results: Patients who received apheresis platelet transfusion had a lower 90-day cumulative survival (78.9% vs. 94.2%, P = 0.009), but had no significant difference in overall survival in the Cox model, compared with those without apheresis platelet transfusion. Units of apheresis platelet transfusion (hazard ratio [HR] = 3.103, 95% confidence interval [CI]: 1.720–5.600, P < 0.001) and preoperative platelet count (HR = 0.170, 95% CI: 0.040–0.730, P = 0.017) impacted 90-day survival independently. Multivariate Cox regression analysis also found that units of red blood cell (RBC) transfusion (HR = 1.036, 95% CI: 1.006–1.067, P = 0.018), recipient's age (HR = 1.045, 95% CI: 1.005–1.086, P = 0.025), and ABO blood group comparison (HR = 2.990, 95% CI: 1.341–6.669, P = 0.007) were independent risk factors for overall survival after LDLT. Conclusions: This study suggested that apheresis platelets were only associated with early mortality but had no impact on overall survival in LDLT. Units of RBC, recipient's age, and ABO group comparison were independent predictors of long-term outcomes.
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- 2018
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18. Analysis of the Characteristics of Pregnancy and Delivery before and after Implementation of the Two-child Policy
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Hong-Xia Zhang, Yang-Yu Zhao, and Yong-Qing Wang
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Cesarean Section Rate ,Pregnancy Complications ,Two-child Policy ,Medicine - Abstract
Background: After the two-child policy is fully implemented, new challenges regarding pregnancy management and the treatment of pregnancy complications will arise. The aim of this study was to analyze the characteristics of pregnancy and delivery before and after the implementation of the two-child policy to make suggestions on the quality assurance of the new era of obstetrics. Methods: In total, 5895 cases of pregnant women who delivered from April 2016 to March 2017 in Peking University Third Hospital served as the study group and 5103 cases of pregnant women who delivered from January to December 2015 served as the control group. The characteristics of pregnancy and delivery were retrospectively analyzed. Results: In the study group, the percentage of pregnant women who were older (over 40 years) (3.6% vs. 2.2%), were multipara (30.3% vs. 17.0%), received irregular prenatal care (1.5% vs. 0.9%), were transferred for treatment from a subordinate hospital (4.4% vs. 2.8%), and were not residents of Beijing (3.8% vs. 2.2%), were significantly increased compared with the control group (P < 0.05). In the study group, the rate of a hypertensive disorder complicating pregnancy (6.4% vs. 5.0%), gestational diabetes mellitus (25.3% vs. 23.1%), dangerous placenta previa (3.0% vs. 2.3%), placental implantation (2.4% vs. 1.8%), and severe postpartum hemorrhage (2.8% vs. 1.9%) was significantly increased compared with the control group (P < 0.05). In the study group, the cesarean section rate during primipara was significantly reduced compared with the control group (42.0% vs. 44.2%). However, the rate during multipara was significantly increased compared with the control group (P < 0.05). Indications for cesarean section in the study group as well as the percentages of scared uterus and placenta previa were significantly increased compared with the control group (P < 0.05). Conclusions: According to the current situation, better methods are needed to strengthen pregnancy and delivery management, reduce the rate of cesarean section, and ensure a positive outcome for mothers and babies.
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- 2018
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19. Association of the gut microbiome and metabolome with wheeze frequency in childhood asthma
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Scott T. Weiss, Nancy Laranjo, Megan Sandel, Babak Momeni, Kathleen Lee-Sarwar, Sandra Dedrick, Diane R. Gold, Robert S. Zeiger, Jessica Lasky-Su, Yang-Yu Liu, Leonard B. Bacharier, George T. O'Connor, Rachel S. Kelly, Augusto A. Litonjua, and Avraham Beigelman
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Childhood asthma ,business.industry ,Immunology ,Gut microbiome ,Asthma ,Gastrointestinal Microbiome ,Feces ,Wheeze ,Child, Preschool ,RNA, Ribosomal, 16S ,medicine ,Metabolome ,Humans ,Metabolomics ,Immunology and Allergy ,medicine.symptom ,business ,Respiratory Sounds - Abstract
While the microbiome has an established role in asthma development, less is known about its contribution to morbidity in children with asthma.In this ancillary study of the Vitamin D Antenatal Asthma Reduction Trial (VDAART), we analyzed the gut microbiome and metabolome of wheeze frequency in children with asthma.Bacterial 16S ribosomal RNA microbiome and untargeted metabolomic profiling were performed on fecal samples collected from 3-year-old children with parent-reported physician-diagnosed asthma. We analyzed wheeze frequency by calculating the proportion of quarterly questionnaires administered between ages 3 and 5 years in which parents reported the child had wheezed (wheeze proportion). Taxa and metabolites associated with wheeze were analyzed by identifying log fold changes with respect to wheeze frequency and correlation/linear regression analyses, respectively. Microbe-metabolite and microbe-microbe correlation networks were compared between subjects with high and low wheeze proportion.Specific taxa, including the genus Veillonella and histidine pathway metabolites, were enriched in subjects with high wheeze proportion. Among wheeze-associated taxa, Veillonella and Oscillospiraceae UCG-005, which was inversely associated with wheeze, were correlated with the greatest number of fecal metabolites. Microbial networks were similar between subjects with low versus high wheeze frequency.Gut microbiome features are associated with wheeze frequency in children with asthma, suggesting an impact of the gut microbiome on morbidity in childhood asthma.
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- 2022
20. Can Dexmedetomidine Improve Arterial Oxygenation and Intrapulmonary Shunt during One-lung Ventilation in Adults Undergoing Thoracic Surgery? A Meta-analysis of Randomized, Placebo-controlled Trials
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Su-Qin Huang, Jian Zhang, Xiong-Xin Zhang, Lu Liu, Yang Yu, Xian-Hui Kang, Xiao-Min Wu, and Sheng-Mei Zhu
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Dexmedetomidine ,Intrapulmonary Shunt ,Meta-analysis ,One-lung Ventilation ,Oxygenation Index ,Medicine - Abstract
Background: One-lung ventilation (OLV) is a common ventilation technology during thoracic surgery that can cause serious clinical problems. We aimed to conduct a meta-analysis to compare oxygenation and intrapulmonary shunt during OLV in adults undergoing thoracic surgery with dexmedetomidine (Dex) versus placebo to assess the influence and safety of using Dex. Methods: Randomized controlled trials comparing lung protection in patients who underwent thoracic surgery with Dex or a placebo were retrieved from PubMed, EMBASE, MEDLINE, Cochrane Library, and China CNKI database. The following information was extracted from the paper: arterial oxygen partial pressure (PaO2), PaO2/inspired oxygen concentration (PaO2/FiO2, oxygenation index [OI]), intrapulmonary shunt (calculated as Qs/Qt), mean arterial pressure (MAP), heart rate (HR), tumor necrosis factor-α (TNF-α), interleukin (IL)-6, superoxide dismutase (SOD), and malondialdehyde (MDA). Results: Fourteen randomized controlled trials were included containing a total of 625 patients. Compared with placebo group, Dex significantly increased PaO2/FiO2(standard mean difference [SMD] = 0.98, 95% confidence interval [CI] [0.72, 1.23], P < 0.00001). Besides, Qs/Qt (SMD= −1.22, 95% CI [−2.20, −0.23], P = 0.020), HR (SMD= −0.69, 95% CI [−1.20, 0.17], P = 0.009), MAP (SMD= −0.44, 95% CI [−0.84, 0.04], P = 0.030), the concentrations of TNF-α (SMD = −1.55, 95% CI [−2.16, −0.95], P
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- 2017
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21. A Novel Imidazo[1,2-a]pyridine Compound Reduces Cell Viability and Induces Apoptosis of HeLa Cells by p53/Bax-Mediated Activation of Mitochondrial Pathway
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Bin Xu, Qiuyi Deng, Yang Yu, Xinjie Yang, Yanwen Li, Hua Cao, and Jianwen Mao
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Cancer Research ,Cell Survival ,Pyridines ,Antineoplastic Agents ,Apoptosis ,Flow cytometry ,HeLa ,Western blot ,medicine ,Humans ,MTT assay ,Viability assay ,Cell Proliferation ,bcl-2-Associated X Protein ,Membrane Potential, Mitochondrial ,Pharmacology ,biology ,medicine.diagnostic_test ,Cell growth ,Chemistry ,Cytochrome c ,biology.organism_classification ,Molecular biology ,biology.protein ,Molecular Medicine ,Tumor Suppressor Protein p53 ,HeLa Cells - Abstract
Background: Despite emerging research on new treatment strategies, chemotherapy remains one of the most important therapeutic modalities for cancers. Imidazopyridines are important targets in organic chemistry and, given their numerous applications, they are worthy of attention. Objective: The objective of this study was to design and synthesize a novel series of imidazo[1,2-a]pyridine-derived compounds and investigate their antitumor effects and the underlying mechanisms. Methods: Imidazo[1,2-a]pyridine-derived compounds were synthesized with new strategies and conventional methods. The antitumor activities of the new compounds were evaluated by MTT assay. Flow cytometry and immunofluorescence were performed to examine the effects of the most effective antiproliferative compound on cell apoptosis. Western blot analysis was used to assess the expression of apoptotic proteins. Results: Fifty-two new imidazo[1,2-a]pyridine compounds were designed and successfully synthesized. The compound, 1-(imidazo[1,2-a]pyridin-3-yl)-2-(naphthalen-2-yl)ethane-1,2-dione, named La23, showed high potential for suppressing the viability of HeLa cells (IC50 15.32 μM). La23 inhibited cell proliferation by inducing cell apoptosis, and it reduced the mitochondrial membrane potential of HeLa cells. Moreover, treatment with La23 appeared to increase the expression of apoptotic-related protein P53, Bax, cleaved caspase-3, and cytochrome c at a low concentration range. Conclusion: The novel imidazo[1,2-a]pyridine compound, La23, was synthesized and it suppressed cell growth by inducing cell apoptosis via the p53/Bax mitochondrial apoptotic pathway.
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- 2022
22. Functional properties of bacterial communities in water and sediment of the eutrophic river-lake system of Poyang Lake, China
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Ze Ren, Xiaodong Qu, Wenqi Peng, Yang Yu, and Min Zhang
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Carbon metabolism ,Nitrogen cycle ,Tributaries ,Bacterial community ,Biogeochemistry ,Eutrophication ,Medicine ,Biology (General) ,QH301-705.5 - Abstract
In river-lake systems, sediment and water column are two distinct habitats harboring different bacterial communities which play a crucial role in biogeochemical processes. In this study, we employed Phylogenetic Investigation of Communities by Reconstruction of Unobserved States to assess the potential functions and functional redundancy of the bacterial communities in sediment and water in a eutrophic river-lake ecosystem, Poyang Lake in China. Bacterial communities in sediment and water had distinct potential functions of carbon, nitrogen, and sulfur metabolisms as well as phosphorus cycle, while the differences between rivers and the lake were inconspicuous. Bacterial communities in sediment had a higher relative abundance of genes associated with carbohydrate metabolism, carbon fixation pathways in prokaryotes, methane metabolism, anammox, nitrogen fixation, and dissimilatory sulfate reduction than that of water column. Bacterial communities in water column were higher in lipid metabolism, assimilatory nitrate reduction, dissimilatory nitrate reduction, phosphonate degradation, and assimilatory sulfate reduction than that of sediment bacterial communities. Furthermore, the variations in functional composition were closely associated to the variations in taxonomic composition in both habitats. In general, the bacterial communities in water column had a lower functional redundancy than in sediment. Moreover, comparing to the overall functions, bacterial communities had a lower functional redundancy of nitrogen metabolism and phosphorus cycle in water column and lower functional redundancy of nitrogen metabolism in sediment. Distance-based redundancy analysis and mantel test revealed close correlations between nutrient factors and functional compositions. The results suggested that bacterial communities in this eutrophic river-lake system of Poyang Lake were vulnerable to nutrient perturbations, especially the bacterial communities in water column. The results enriched our understanding of the bacterial communities and major biogeochemical processes in the eutrophic river-lake ecosystems.
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- 2019
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23. Optical properties and radiation stability of SiO2/ZnO composite pigment prepared by co-sintering method
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Yun Yu, Junge Chen, Aihu Feng, Mi Le, Huixin Xiu, and Yang Yu
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Materials science ,Proton ,Process Chemistry and Technology ,Composite number ,Relaxation (NMR) ,Sintering ,engineering.material ,Radiation ,medicine.disease_cause ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Coating ,Chemical engineering ,Materials Chemistry ,Ceramics and Composites ,engineering ,medicine ,Thermal emittance ,Ultraviolet - Abstract
Thermal control coatings (TCCs) are an essential part of the thermal control systems in the spacecraft. Solar absorptance and emittance are the key performance parameters of TCCs. To develop an ultra-low solar absorption and stable inorganic TCCs for surface radiator, different TCCs were prepared by co-sintering ZnO and SiO2 nanoparticles to form Zn2SiO4/SiO2 pigment in this work, and the optical properties and radiation stability were systematically studied. It is found that the coating based on composite pigment has high reflectivity in the ultraviolet band and excellent optical performance possessing the low solar absorption of 0.06. In addition, the Zn2SiO4/SiO2 coating demonstrates the highest proton and electron radiation stability because that SiO2 between Zn2SiO4 particles acts as the relaxation center of the defects caused by radiation.
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- 2022
24. An improved grain-based numerical manifold method to simulate deformation, damage and fracturing of rocks at the grain size level
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Guang-lei Zhou, Yong Zhao, Xian-yang Yu, Heinz Konietzky, Tao Xu, Zhen Heng, and Wancheng Zhu
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Materials science ,Applied Mathematics ,General Engineering ,Stiffness ,Boundary (topology) ,Mechanics ,Deformation (meteorology) ,Grain size ,law.invention ,Computational Mathematics ,law ,medicine ,Cohesion (geology) ,Grain boundary ,medicine.symptom ,Voronoi diagram ,Manifold (fluid mechanics) ,Analysis - Abstract
An improved grain-based numerical manifold method (NMM) is developed to investigate deformation and damage of intact rocks at the meso‑scale. The grain boundaries are embedded into the numerical manifold method using a random Voronoi tessellation technique to approximate the microstructure of rocks at the meso‑scale. To enhance efficiency, an improved contact loop updating algorithm is proposed, which only preserves the corners of polygonal blocks and deletes the rest of the loop boundary nodes, thus greatly reducing the number of loop nodes involved in contact retrieval. An interface contact model considering cohesion and tensile strength between rock grains is incorporated into the numerical manifold method to simulate fracturing. With the newly developed grain-based numerical manifold method, Brazilian tests and uniaxial compression tests are numerically simulated to validate failure pattern and macroscopic response against laboratory tests. Sensitivity analysis is conducted using the proposed model to further investigate the influence of different number of grains and different stiffness ratio on the macroscopic response of rocks. The results indicate that the improved grain-based numerical manifold method can be effectively used to study deformation, damage and fracturing of rocks at the meso‑scale.
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- 2022
25. Secondary Infections After Diagnosis of Severe Radiation Pneumonitis (SRP) Among Patients With Non-Small Cell Lung Cancer: Pathogen Distributions, Choice of Empirical Antibiotics, and the Value of Empirical Antifungal Treatment
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Lin Zhou, Bingwen Zou, T. Mei, Xiaojuan Zhou, Xiaoman Tian, Yang Yu, You Lu, Min Quan, Libo Yan, Xuexi Yang, Yong Xu, Yongmei Liu, Jianxin Xue, Youling Gong, and Qianyue Deng
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Cancer Research ,medicine.medical_specialty ,Antifungal Agents ,Lung Neoplasms ,medicine.drug_class ,Secondary infection ,Antibiotics ,Antifungal drug ,Microbial Sensitivity Tests ,Antibiotic resistance ,Carcinoma, Non-Small-Cell Lung ,Internal medicine ,medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,Lung cancer ,Retrospective Studies ,Radiation ,Coinfection ,business.industry ,medicine.disease ,Anti-Bacterial Agents ,Radiation Pneumonitis ,Pneumonia ,Oncology ,Klebsiella pneumonia ,business - Abstract
Purpose This study aimed to assess pathogen distributions and antimicrobial sensitivity characteristics in patients with non-small cell lung cancer (NSCLC) with severe radiation pneumonitis (SRP) and secondary infections. Methods and Materials Data from 1746 patients with NSCLC and SRP after thoracic radiation therapy from January 2009 to December 2020 were retrospectively analyzed. Pneumonia incidence, causative pathogens, and antibiotic resistance characteristics in patients with secondary lung infections were analyzed. Risk factors associated with mortality were identified through univariate and multivariate analyses. Antifungal drug efficacy and duration-related effects were assessed with Forest plots and receiver operating characteristic curves. Results Overall, 44.5% of patients with NSCLC and SRP (777 of 1746 patients) were diagnosed with secondary lung infections. In total, 899 bacterial strains were isolated from these patients, with Acinetobacter baumannii (n = 206; 27%), Klebsiella pneumonia (n = 200; 26.2%), and Pseudomonas aeruginosa (n = 104; 13.6%) being the most common. Carbapenems and cefoperazone-sulbactam resistance rates of 52.7% and 32.2%, 28.8% and 26.4%, and 23.7% and 20.2% were observed for these isolates, respectively. Infection-related deaths occurred in 22.4% of patients with SRP. Independent risk factors for infection-related death included poor performance status scores, inappropriate empirical antimicrobial treatment, bacteria/fungal coinfection, and lack of empirical antifungal treatment. Receiver operating characteristic curves showed that the cutoff value of empirical antifungal treatment duration was 9 (area under the curve: 0.819). Conclusions For patients with SRP and secondary lung infections, appropriate empirical antimicrobial treatment could decrease infection-related mortality, and cefoperazone-sulbactam may be an appropriate antibacterial drug. Empirical antifungal treatment for a minimum of 9 days might contribute to better outcomes. Although this represents a promising treatment approach for patients with SRP and secondary lung infections before antibacterial susceptibility testing, further prospective validation is essential.
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- 2022
26. The practice of comprehensive intervention of children′s oral diseases
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LIU Hu, ZHAI Yuan-yuan, YANG Yu, and LIANG Yuan-yuan
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Comprehensive intervention ,Pit and fissure sealant ,Rate of sealant retention ,Oral health education ,Organizational form ,Medicine - Abstract
Objective To evaluate the effect of the comprehensive intervention of the oral health education for children aged 6 to 12 years.Methods The oral health knowledge questionnaire was used to evaluatethe children′s awareness about oral health and the correct rate of tooth-brushing before and after 12-month comprehensive intervention. Two organizational forms about pit and fissure sealant were adopted. In city school, students were brought by their parents to the hospital to do pit and fissure sealant, while in rural school, hospital medical team went to schools to do pit and fissure sealant for the students. Compare the sealants retention rate in 12-month follow-up between two forms. The total sealants retention rate among 6-month, 12-month and 24-month follow-up were compared.Results Before and after oral health education guidance, the rate of children′s oral health awareness was 68% and 96%, and the correct rate of tooth-brushing was 64% and 92% respectively (P
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- 2016
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27. Breviscapine alleviates NASH by inhibiting TGF‐β‐activated kinase 1‐dependent signaling
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Xin Ding, Shenghua Piao, Yang Yu, Tian Lan, Shuo Jiang, Jiao Guo, Qing Zhu, Xue Xiao, Yiqi Yang, Qiqing Weng, Xianglu Rong, Weixuan Wang, Lexun Wang, Sha Hu, Song Tian, Jing Zhang, Haonan Li, and Duosheng Luo
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Liver Cirrhosis ,Pharmacology ,Diet, High-Fat ,Mice ,Non-alcoholic Fatty Liver Disease ,Fibrosis ,Nonalcoholic fatty liver disease ,Animals ,Medicine ,Flavonoids ,Inflammation ,Liver injury ,Hepatology ,business.industry ,Fatty liver ,Lipid metabolism ,Lipid Metabolism ,MAP Kinase Kinase Kinases ,medicine.disease ,Mice, Inbred C57BL ,Molecular Docking Simulation ,Liver ,Hepatoprotection ,Lipotoxicity ,Steatosis ,business - Abstract
Background & aims Nonalcoholic fatty liver disease (NAFLD) is a key component of metabolic syndrome ranging from nonalcoholic fatty liver (NAFL) to nonalcoholic steatohepatitis (NASH) and is now becoming the leading cause of cirrhosis and hepatocellular carcinoma worldwide. However, due to the complex and unclear pathophysiological mechanism, there are no specific approved agents for treating NASH. Breviscapine, a natural flavonoid prescription drug isolated from the traditional Chinese herb Erigeron breviscapus, exhibits a wide range of pharmacological properties, including effects on metabolism. However, the anti-NASH efficacy and mechanisms of breviscapine have not yet been characterized. Approach & results We evaluated the effects of breviscapine on the development of hepatic steatosis, inflammation and fibrosis in vivo and in vitro under metabolic stress. Breviscapine treatment significantly reduced lipid accumulation, inflammatory cell infiltration, liver injury and fibrosis in mice fed a high-fat diet (HFD), a high-fat/high-cholesterol (HFHC) diet, or a methionine- and choline-deficient (MCD) diet. In addition, breviscapine attenuated lipid accumulation, inflammation and lipotoxicity in hepatocytes undergoing metabolic stress. RNA sequencing and multiomics analyses further indicated that the key mechanism linking the anti-NASH effects of breviscapine was inhibition of TAK1 phosphorylation and the subsequent MAPK signaling cascade. Treatment with the TAK1 inhibitor 5Z-7-oxozeaenol abrogated breviscapine-mediated hepatoprotection under metabolic stress. Molecular docking illustrated that breviscapine directly bound to TAK1. Conclusion Breviscapine prevents metabolic stress-induced NASH progression through direct inhibition of TAK1 signaling. Breviscapine might be a novel therapeutic candidate for the treatment of NASH.
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- 2021
28. Detection and significance of main anchoring villus in early pregnancy
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Qing Xie, Yi Zhang, Qi Zhang, Chengji Wang, Zhikun Zhang, and Yang Yu
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Pregnancy ,Fetus ,business.industry ,digestive, oral, and skin physiology ,Ultrasound ,Obstetrics and Gynecology ,Anchoring ,Gestational age ,Gestational Age ,Embryo ,General Medicine ,medicine.disease ,Chorionic Gonadotropin ,digestive system ,Crown-Rump Length ,Ultrasonography, Prenatal ,Human chorionic gonadotropin ,Andrology ,Pregnancy Trimester, First ,medicine ,Humans ,Gestation ,Female ,business - Abstract
OBJECTIVE To find and explore the relationship among the length of the embryo's main anchoring villus and the week of gestation, embryonic crown-rump length (CRL), and maternal blood human chorionic gonadotropin (hCG) during early pregnancy using ultrasound. METHODS A total of 108 women with singleton pregnancies underwent ultrasound examination of the fetus during the early months of their pregnancy (5+5 -13+6 weeks of pregnancy). The main anchoring villus was detected using HD-Flow and SlowflowHD mode ultrasound. The detection rate and length of the main anchoring villus were recorded. The correlations among main anchoring villus with gestational weeks, CRL, and blood hCG were obtained. RESULTS The main anchoring villus was detected in all women (108/108). It could be found throughout the early gestational weeks from as early as 4+6 weeks. The length of the main anchoring villus increased with gestational age, and was positively correlated with CRL and the logarithm of hCG (P
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- 2021
29. Identification of a novel antifungal backbone of naphthalimide thiazoles with synergistic potential for chemical and dynamic treatment
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Yang Yu, Bo Fang, Peng-Li Zhang, Cheng-He Zhou, and Gopala Lavanya
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Pharmacology ,Antifungal ,Antifungal Agents ,Molecular Structure ,medicine.drug_class ,Chemistry ,Antifungal drugs ,Microbial Sensitivity Tests ,Combinatorial chemistry ,Naphthalimides ,Thiazoles ,chemistry.chemical_compound ,Drug Discovery ,medicine ,Molecular Medicine ,Identification (biology) ,Candida tropicalis ,High incidence ,Thiazole - Abstract
Aim: The high incidence and prevalence of fungal infections call for new antifungal drugs. This work was to develop naphthalimide thiazoles as potential antifungal agents. Results & methodology: These compounds showed significant antifungal potency toward some tested fungi. Especially, naphthalimide thiazole 4h with excellent anti- Candida tropicalis efficacy possessed good hemolysis level, low toxicity and no obvious resistance. Deciphering the mechanism showed that 4h interacted with DNA and disrupted the antioxidant defense system of C. tropicalis. Compound 4h also triggered membrane depolarization, leakage of cytoplasmic contents and LDH inhibition. Simultaneously, 4h rendered metabolic inactivation and eradicated the formed biofilms of C. tropicalis. Conclusion: The multifaceted synergistic effect initiated by naphthalimide thiazoles is a reasonable treatment window for prospective development.
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- 2021
30. Triple-Level-Cell/Single-Level-Cell Mix-Mode Operation Induced Data Retention Degradation in 3-D NAND Flash Memories
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Yu-Cheng Hsu, Wei Lin, Chih-Yuan Tseng, An-Chang Liu, Yang Yu-Siang, and Yu-Heng Liu
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Materials science ,business.industry ,Cell ,Triple level cell ,Mode (statistics) ,NAND gate ,Single level ,Electronic, Optical and Magnetic Materials ,Flash (photography) ,medicine.anatomical_structure ,medicine ,Degradation (geology) ,Optoelectronics ,Electrical and Electronic Engineering ,Data retention ,business - Published
- 2021
31. Bacteroides fragilis restricts colitis-associated cancer via negative regulation of the NLRP3 axis
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Yuqing Zhou, Shishuo Sun, Yang Yu, Tong Hu, Xinyu Shao, Huiyu Wang, Yizhou Yao, and Chunli Zhou
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Male ,Cancer Research ,Colorectal cancer ,Butyrate ,Gut flora ,Proinflammatory cytokine ,Bacteroides fragilis ,Mice ,NLR Family, Pyrin Domain-Containing 3 Protein ,Animals ,Humans ,Medicine ,biology ,business.industry ,Macrophages ,Cancer ,Fatty Acids, Volatile ,biology.organism_classification ,medicine.disease ,Ulcerative colitis ,Gastrointestinal Microbiome ,Mice, Inbred C57BL ,Transplantation ,Butyrates ,Oncology ,Immunology ,Dysbiosis ,Colitis, Ulcerative ,Colitis-Associated Neoplasms ,business - Abstract
Patients with persistent ulcerative colitis (UC) are at a higher risk of developing colitis-associated cancer (CAC). Previous studies have reported that intestinal microbiota disturbance plays an important role in the process of CAC development in patients with UC, indicating that targeted intervention of intestinal microbiota and its metabolites may be a potential therapeutic strategy. Gut microbiota in the process of colorectal cancer development in UC patients was analyzed using the gutMEGA database and verified in fecal samples. The abundance of Bacteroides fragilis reduced significantly in the process of colitis associated cancer development. Broad-spectrum antibiotics (BSAB) intervene with the intestinal microbiota of mice and accelerate the process of colon cancer development. However, gavage transplantation with B. fragilis can effectively reverse the effects of BSAB. In the intestinal tract, B. fragilis promotes the secretion of short-chain fatty acids (SCFAs). Subsequently, SCFAs, especially butyrate, negatively regulate the inflammatory signaling pathway mediated by NLRP3 to inhibit the activation of macrophages and the secretion of proinflammatory mediators such as IL-18 and IL-1β, reducing the level of intestinal inflammation and restricting CAC development. In conclusion, colonization with B. fragilis has been shown to be effective in ameliorating intestinal epithelial damage caused by chronic inflammation and preventing the development of colonic tumors. Thus, it can be a therapeutic intervention strategy with good clinical application prospects.
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- 2021
32. Regulation of Growth and Salt Resistance in Cucumber Seedlings by Hydrogen-Rich Water
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Haina Zhang, Yang Yu, Hongyun Xing, Wang Xiaoyan, Lei Fan, Haiyan Fan, Xiaoyan Liu, Xiangnan Meng, and Na Cui
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Antioxidant ,biology ,Osmotic shock ,medicine.medical_treatment ,Plant physiology ,Plant Science ,biology.organism_classification ,Malondialdehyde ,APX ,Lipid peroxidation ,Horticulture ,chemistry.chemical_compound ,chemistry ,Shoot ,medicine ,Agronomy and Crop Science ,Cucumis - Abstract
The secondary salinization of soil in facility agriculture is becoming increasingly serious. Cucumber is a moderately salt-sensitive crop, but because of the weak root system and poor resistance to salt stress in cucumber seedlings, it is sensitive to the accumulation of salt in facility soils. Hydrogen, a selective antioxidant and signal molecule, is nontoxic and harmless, and can repair damage in plants under stress. Therefore, the aim of the present paper was to understand the specific mechanism by which hydrogen-rich water (HRW) alleviated salt stress in cucumber seedlings (Cucumis sativus L.). Our results showed that the addition of 50% saturation HRW significantly promoted seedling growth, development and photosynthetic efficiency. Pretreatment with HRW significantly alleviated salt stress symptoms, including the inhibition of fresh and dry weight, root length, lateral roots, and the root/shoot ratio of cucumber seedlings. Pretreatment with HRW increased the chlorophyll content, chlorophyll a/b ratio, and photochemical reaction efficiency, and reduced energy dissipation. These responses to HRW pretreatment were consistent with significant decreases in the superoxide anion, hydrogen peroxide, and malondialdehyde contents and the degree of lipid peroxidation, and increases in the activities of SOD, POD, CAT, APX and GR, and the contents of ASA and GSH in cucumber seedlings under salt stress. In addition, HRW pretreatment under salt stress inhibited the expression of the protein kinase ROP1, which promoted the production of reactive oxygen, but upregulated the protein kinase LecRLK. The transcription factor TGA5, which was involved in osmotic stress, ion stress and ROS clearance, and the expression of NHX1 and SOS2, which were parts of the SOS signaling pathway. HRW enhanced the expression of genes that encoded antioxidant enzymes, including SOD, CAT and POD, and the expressions of GR and APX2, which were key genes in the ASA-GSH cycle under salt stress. Taken together, these results suggested that HRW enhanced the active oxygen scavenging ability in cucumber seedlings, promoted the redox balance in cells, and reduced the degree of oxidative damage in plants under salt stress by reducing the content of active oxygen. Therefore, the application of HRW might be a promising strategy for improving salt stress tolerance in cucumber seedlings.
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- 2021
33. Fine-needle aspiration cytology of an intrathyroidal nodule diagnosed as squamous cell carcinoma: A case report
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Jin-Yang Yu, Ying Zhang, and Zhe Wang
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Pathology ,medicine.medical_specialty ,business.industry ,Nodule (medicine) ,Diagnosis by fine-needle aspiration ,General Medicine ,Aspiration cytology ,Cytology of squamous cell carcinoma in thyroid ,stomatognathic diseases ,Fine needle aspiration cytology ,Intrathyroidal squamous cell carcinoma ,Case report ,Fine-needle aspiration cytology ,medicine ,Cytology of papillary thyroid carcinoma ,Basal cell ,medicine.symptom ,business - Abstract
BACKGROUND Both squamous cell carcinoma (SCC) and papillary thyroid carcinoma (PTC) are common malignant tumors in the neck. However, seldom has SCC of the thyroid been diagnosed. Further, cytological features of SCC and PTC have rarely been reported. The significance of fine-needle aspiration cytology (FNAC) in the diagnosis of neck masses has been established. Herein, we present an exceedingly rare case of an intrathyroidal SCC diagnosed using FNAC, along with its cytological features. CASE SUMMARY A 66-year-old man presented with a left-sided neck mass. Ultrasound examination showed an ill-defined nodule. The appearance was hypoechoic with a few hyperechoic spots. FNAC of the left thyroid nodule was performed. A cellular smear was obtained, and it showed a large number of neoplastic cells with rich cytoplasm and poor cell adhesion. Tumor cell nuclei showed coarse nuclear chromatin and a few enlarged prominent nucleoli. An increased nuclear/cytoplasm ratio was observed. Thus, malignancy was diagnosed without a confirmed tumor type. Percutaneous tumor biopsy was performed to make a definite diagnosis. The tumor cells showed typical squamous cell characteristics. CONCLUSION Head and neck SCC and PTC have different cytologies. Measures are needed to ensure accurate diagnosis using FNAC.
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- 2021
34. Posthepatectomy jaundice induced by paroxysmal nocturnal hemoglobinuria: A case report
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Meng Wang, Jian-Feng Cui, Xiao-Dong Xie, Guang-Xu Jing, Hong-Yin Liang, and Yang Yu
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medicine.medical_specialty ,business.industry ,Jaundice ,General Medicine ,medicine.disease ,Gastroenterology ,hemic and lymphatic diseases ,Internal medicine ,Case report ,Paroxysmal nocturnal hemoglobinuria ,medicine ,Hepatectomy ,medicine.symptom ,business - Abstract
BACKGROUND Jaundice is a major manifestation of posthepatectomy liver failure, a feared complication after hepatic resection. Herein, we report a case of posthepatectomy jaundice that was not caused by liver failure but by paroxysmal nocturnal hemoglobinuria (PNH)-induced hemolysis. CASE SUMMARY A 56-year-old woman underwent right hepatectomy and biliary tract exploration surgery due to hepatic duct stones. Prior to surgery, the patient was mildly anemic. The direct antiglobulin test was negative. A bone marrow biopsy showed mild histiocyte hyperplasia. After surgery, the patient suffered a progressive increase in serum bilirubin. Meanwhile, the patient developed hemolytic symptoms after blood transfusion. She was ultimately diagnosed with PNH. PNH is a rare bone marrow failure disorder that manifests as complement-dependent intravascular hemolysis with varying severity. After steroid treatment, the patient’s jaundice gradually decreased, and the patient was discharged on the 35th postoperative day. CONCLUSION PNH-induced hemolysis is a rare cause of posthepatectomy jaundice. It should be suspected in patients having posthepatectomy hyperbilirubinemia without other signs of liver failure. Steroid therapy can be considered for the treatment of PNH in such cases.
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- 2021
35. Increased Risk of Postpartum Depression in Women With Allergic Rhinitis During Pregnancy: A Population-Based Case-Control Study
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Li-Xin Wang, Yang Yu, Huan-Yu Lin, Zhi-Chao Yang, and Liang-Chun Shih
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Postpartum depression ,medicine.medical_specialty ,Multivariate analysis ,Population ,Depression, Postpartum ,Pregnancy ,Risk Factors ,Humans ,Medicine ,education ,Depression (differential diagnoses) ,Asthma ,education.field_of_study ,business.industry ,Obstetrics ,Medical record ,Case-control study ,General Medicine ,medicine.disease ,Rhinitis, Allergic ,Otorhinolaryngology ,Case-Control Studies ,Multivariate Analysis ,Female ,business - Abstract
Objectives: Allergic rhinitis (AR) is associated with increased risk of major depression in the general population, however, no previous study has evaluated its role among pregnant women. We aimed to investigate the potential impact of AR during pregnancy on the development of postpartum depression (PPD). Methods: This is a population-based case-control study. Data were retrieved from the National Health Insurance Research Database (NHIRD). Medical records of a total of 199 470 deliveries during 2000 and 2010 were identified. Among which, 1416 women with PPD within 12 months after delivery were classified as the case group, while 198 054 women without PPD after delivery formed the control group. Univariate and multivariate regression analyses were conducted to determine the associations between AR during pregnancies and other study variables with PPD. Results: AR during pregnancy was found in 9.53% women who developed PPD and 5.44% in women without PPD. After adjusting for age at delivery, income level, various pregnancy and delivery-related conditions, asthma, atopic dermatitis and other medical comorbidities in the multivariate analysis, AR was significantly associated with increased odds of PPD (aOR: 1.498, 95% CI: 1.222-1.836). Conclusion: AR during pregnancy was independently and significantly associated with an approximately 50% increased risk of PPD among women giving birth. Closely monitoring of AR is warranted in the future in order to optimize mother and child outcomes after delivery.
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- 2021
36. Construction of Anatomical Structure-specific Developmental Dynamic Networks for Human Brain on Multiple Omics Levels
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Li Keshen, Wang Yingying, Yang Yu, and Liu Jianfeng
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Structure (mathematical logic) ,Computational Mathematics ,medicine.anatomical_structure ,Genetics ,medicine ,Computational biology ,Human brain ,Biology ,Omics ,Molecular Biology ,Biochemistry - Abstract
Background: Human brain development is a series of complex processes exhibiting profound changes from gestation to adulthood. Objective: We aimed to construct dynamic developmental networks for each anatomical structure of the human brain based on omics’ levels in order to gain a new systematical brain map on the molecular level. Methods: We performed the brain development analyses by constructing dynamical networks between adjacent time points on different grouping levels of anatomical structures. The gene-time networks were first built to obtain the developing brain dynamical maps on transcriptome level. Then miRNA-mRNA networks and protein-protein networks were constructed by integrating the information from miRNomics and proteomics. The time and structure-specific biomarkers were filtered based on analyses of topological characters. Results: The most dramatic developmental time and structure were fetal-infancy and telencephalon, respectively. Cortex was the key developmental region in ‘late fetal and neonatal’ and ‘early infancy’. The development of the temporal lobe was different from other lobes since the significant changes of molecules were found only in the comparison pair ‘early fetal-early mid-fetal’ and ‘adolescence-young adulthood’. Interestingly, the changes among different brain structures inside adolescence and adulthood were bigger than other time points. hsa-miR-548c-3p and H3C2 may be new brain development indicators considering their key roles in networks. Conclusion: To our knowledge, this study is the first report of dynamical brain development maps for different anatomical structures on multiple omics. The results provide a new sight of brain development in a systematical way which may provide a more accurate understanding of the human brain.
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- 2021
37. Polarization of rheumatoid macrophages is regulated by the CDKN2B-AS1/ MIR497/TXNIP axis
- Author
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Guanlei Liu, Jianzhong Xu, Gu Chenxi, Yu Li, and Yang Yu
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Adult ,Male ,Primary Cell Culture ,Immunology ,Macrophage polarization ,Cell Separation ,CD16 ,Flow cytometry ,Arthritis, Rheumatoid ,medicine ,Humans ,Immunology and Allergy ,Cells, Cultured ,Aged ,Gene knockdown ,CD40 ,medicine.diagnostic_test ,biology ,Chemistry ,Macrophages ,Macrophage Activation ,Middle Aged ,Flow Cytometry ,Healthy Volunteers ,MicroRNAs ,Case-Control Studies ,Gene Knockdown Techniques ,Cancer research ,biology.protein ,Female ,RNA, Long Noncoding ,Carrier Proteins ,CD163 ,TXNIP ,CD80 - Abstract
The polarization of macrophages plays a critical role in the pathophysiology of rheumatoid arthritis. The macrophages can have pro-inflammatory M1 polarization and various types of alternative anti-inflammatory M2 polarization. Our preliminary results showed that the CDKN2B-AS1/MIR497/TXNIP axis might regulate macrophages of rheumatoid arthritis patients. Therefore, we hypothesized that this axis regulated the polarization of rheumatoid macrophages. Flow cytometry was used to determine the surface polarization markers in M1 or M2 macrophages from healthy donors and rheumatoid arthritis patients. The QPCR and Western Blotting were used to compare the expression of the CDKN2B-AS1/MIR497/TXNIP axis in these macrophages. We Knocked down and overexpressed the axis in the macrophage cell line MD to test its roles in macrophage polarization. Compared to cells from healthy donors, cells from rheumatoid arthritis patients expressed higher levels of CD40 and CD80 and lower levels of CD16, CD163, CD206, and CD200R after polarization, they also expressed higher CDKN2B-AS1, lower MIR497, and higher TXNIP. In macrophages from healthy donors, there was no correlation among CDKN2B-AS1, MIR497, and TXNIP. But in macrophages from patients, there were significant correlations. The CDKN2B-AS1 knockdown, MIR497 mimics suppressed the M1 polarization but promoted the M2 polarization in MD cells, while the MIR497 knockdown and the TXNIP overexpression did the opposite. This study demonstrated that elevated CDKN2B-AS1 in macrophages promotes the M1 polarization and inhibited the M2 polarization of macrophages by the CDKN2B-AS1/ MIR497/TXNIP axis.
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- 2021
38. Not using a tourniquet is superior to tourniquet use for high tibial osteotomy: a prospective, randomised controlled trial
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Zhuang Miao, Kaifei Han, Desu Luo, Mingxing Liu, Lei Li, Peilai Liu, Qunshan Lu, Xiangshuai Zhang, Songlin Li, Wenwei Qian, and Yang Yu
- Subjects
medicine.medical_specialty ,Tourniquet ,business.industry ,Visual analogue scale ,equipment and supplies ,law.invention ,body regions ,surgical procedures, operative ,High tibial osteotomy ,Randomized controlled trial ,Blood loss ,law ,Anesthesia ,Orthopedic surgery ,Medicine ,Orthopedics and Sports Medicine ,Surgery ,business ,Prospective cohort study ,Tranexamic acid ,medicine.drug - Abstract
Purpose Tourniquets are routinely used in high tibial osteotomy (HTO). However, research on the necessity of tourniquets during HTO is lacking. This study was designed to investigate the necessity of tourniquets in HTO. Methods This was a prospective study that included patients who underwent HTO at the same hospital. The patients were randomised into Group A (non-tourniquet, n = 45) and Group B (tourniquet, n = 45). Same surgical techniques and haemostatic methods were used in the two groups. Results All patients were followed up for more than three months. There was no difference in operation time, and no intra-operative vascular or nerve damage occurred in either group. The hospital stay was shorter in group A than in group B (p 0.05). The post-operative visual analogue scale (VAS) pain scores and calf swelling were lower in group A (p 0.05). Conclusion Tourniquet use during HTO does not reduce post-operative blood loss, operation time or intra-operative complications, but not using a tourniquet shortens the hospital stay and reduces the post-operative usage of morphine and tourniquet-related complications, which promotes early recovery of knee function.
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- 2021
39. (±)-Stagonosporopsin A, stagonosporopsin B and stagonosporopsin C, antibacterial metabolites produced by endophytic fungus Stagonosporopsis oculihominis
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Hui-Yuan Li, Jing-yi Yue, Guo-Kai Wang, Ju-Tao Wang, Rao Rao, and Yang Yu
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Circular dichroism ,Natural product ,biology ,Stereochemistry ,Chemistry ,Dendrobium huoshanense ,Plant Science ,Endophytic fungus ,medicine.disease_cause ,biology.organism_classification ,Biochemistry ,chemistry.chemical_compound ,Staphylococcus aureus ,Stagonosporopsis ,medicine ,Enantiomer ,Agronomy and Crop Science ,Biotechnology - Abstract
A successive investigation of the endophytic fungus Stagonosporopsis oculihominis isolated from Dendrobium huoshanense led to the discovery of two undescribed secondary metabolites, (±)-stagonosporopsin A and stagonosporopsin B, and a new natural product, stagonosporopsin C. (±)-stagonosporopsin A is a pair of enantiomer. The structures of the isolated compounds, including their absolute configurations, were elucidated based on extensive spectroscopic analyses, electronic circular dichroism (ECD) calculations and X-ray diffraction. Stagonosporopsin C exhibited moderate inhibitory activity against Staphylococcus aureus subsp. aureus ATCC29213 with an MIC50 value of 41.3 μM.
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- 2021
40. Peripheral blood microbial signatures in current and former smokers
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Jarrett D. Morrow, Craig P. Hersh, Robert P. Chase, Sool Lee, Jeong H. Yun, Yang-Yu Liu, and Peter J. Castaldi
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Male ,Firmicutes ,Science ,Disease ,Genome ,Article ,Pulmonary Disease, Chronic Obstructive ,Meta-Analysis as Topic ,Sepsis ,White blood cell ,medicine ,Sequencing ,Humans ,Genetic Predisposition to Disease ,Microbiome ,Lung ,Aged ,Aged, 80 and over ,Genetics ,COPD ,Smokers ,Multidisciplinary ,Host Microbial Interactions ,biology ,Microbiota ,Smoking ,Human microbiome ,Bacteroidetes ,Genomics ,Middle Aged ,biology.organism_classification ,medicine.disease ,Respiratory Function Tests ,medicine.anatomical_structure ,Host-Pathogen Interactions ,Medicine ,Female ,Gene expression ,Disease Susceptibility ,Microbial genetics ,Follow-Up Studies - Abstract
The human microbiome has a role in the development of multiple diseases. Individual microbiome profiles are highly personalized, though many species are shared. Understanding the relationship between the human microbiome and disease may inform future individualized treatments. We hypothesize the blood microbiome signature may be a surrogate for some lung microbial characteristics. We sought associations between the blood microbiome signature and lung-relevant host factors. Based on reads not mapped to the human genome, we detected microbial nucleic acids through secondary use of peripheral blood RNA-sequencing from 2,590 current and former smokers with and without chronic obstructive pulmonary disease (COPD) from the COPDGene study. We used the Genome Analysis Toolkit (GATK) microbial pipeline PathSeq to infer microbial profiles. We tested associations between the inferred profiles and lung disease relevant phenotypes and examined links to host gene expression pathways. We replicated our analyses using a second independent set of blood RNA-seq data from 1,065 COPDGene study subjects and performed a meta-analysis across the two studies. The four phyla with highest abundance across all subjects were Proteobacteria, Actinobacteria, Firmicutes and Bacteroidetes. In our meta-analysis, we observed associations (q-value Acinetobacter, Serratia, Streptococcus and Bacillus inferred abundances and Modified Medical Research Council (mMRC) dyspnea score. Current smoking status was associated (q Acinetobacter, Serratia and Cutibacterium abundance. All 12 taxa investigated were associated with at least one white blood cell distribution variable. Abundance for nine of the 12 taxa was associated with sex, and seven of the 12 taxa were associated with race. Host-microbiome interaction analysis revealed clustering of genera associated with mMRC dyspnea score and smoking status, through shared links to several host pathways. This study is the first to identify a bacterial microbiome signature in the peripheral blood of current and former smokers. Understanding the relationships between systemic microbial signatures and lung-related phenotypes may inform novel interventions and aid understanding of the systemic effects of smoking.
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- 2021
41. Development of improved SRC-3 inhibitors as breast cancer therapeutic agents
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Jin Wang, Dong Lu, Xiaobin Yu, David M. Lonard, David L. Cardenas, Xiaohui Peng, Prashi Jain, Li Qin, Yang Yu, Bert W. O'Malley, Jianwei Chen, and Jianming Xu
- Subjects
Cancer Research ,Epithelial-Mesenchymal Transition ,Endocrinology, Diabetes and Metabolism ,Breast Neoplasms ,Article ,Metastasis ,Nuclear Receptor Coactivator 3 ,Mice ,Endocrinology ,Breast cancer ,Cell Movement ,Cell Line, Tumor ,medicine ,Animals ,Humans ,Receptor ,Transcription factor ,Cell Proliferation ,business.industry ,Cancer ,Oncogenes ,medicine.disease ,Xenograft Model Antitumor Assays ,Oncology ,Nuclear receptor ,Tumor progression ,Cancer research ,Female ,business ,Proto-oncogene tyrosine-protein kinase Src - Abstract
Steroid receptor coactivators (SRCs) possess specific and distinct oncogenic roles in the initiation of cancer and in its progression to a more aggressive disease. These coactivators interact with nuclear receptors and other transcription factors to boost transcription of multiple genes, which potentiate cancer cell proliferation, migration, invasion, tumor angiogenesis and epithelial–mesenchymal transition (EMT). Targeting SRCs using small molecule inhibitors (SMIs) is a promising approach to control cancer progression and metastasis. By high-throughput screening analysis, we recently identified SI-2 as a potent SRC SMI. To develop therapeutic agents, SI-10 and SI-12, the SI-2 analogs are synthesized that incorporate the addition of F atoms to the SI-2 chemical structure. As a result, these analogs exhibit a significantly prolonged plasma half-life, minimal toxicity and improved hERG activity. Biological functional analysis showed that SI-10 and SI-12 treatment (5–50 nM) can significantly inhibit viability, migration and invasion of breast cancer cells in vitro and repress the growth of breast cancer PDX organoids. Treatment of mice with 10 mg/kg/day of either SI-10 or SI-12 was sufficient to repress the growth of xenograft tumors derived from MDA-MB-231 and LM2 cells. Furthermore, in spontaneous and experimental metastasis mouse models developed from MDA-MB-231 and LM2 cells, respectively, SI-10 and SI-12 effectively inhibited the progression of breast cancer lung metastasis. These results demonstrate that SI-10 and SI-12 are promising therapeutic agents and are specifically effective in blocking tumor metastasis, a key point in tumor progression to a more lethal state that results in patient mortality in the majority of cases.
- Published
- 2021
42. C Deletion at the re74650330 Locus of the SLC39A8 Gene (rs74650330) Increases the Risk of Coronary Artery Disease in Individuals with Low-Density Lipoprotein Cholesterol Levels
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Tianhong Yu, Lili Pan, Yang Yu, Jun Zhang, and Guanghua Luo
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medicine.medical_specialty ,business.industry ,Low density lipoprotein cholesterol ,Single-nucleotide polymorphism ,Locus (genetics) ,General Medicine ,medicine.disease ,Coronary artery disease ,Blood pressure ,Endocrinology ,Internal medicine ,Disease risk ,Medicine ,business ,Gene ,Body mass index ,Genetics (clinical) - Abstract
Background: Genetic variants of the SLC39A8 gene are associated with several cardiovascular disease risk factors, including body mass index, systolic blood pressure (SBP), diastolic blood pressure ...
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- 2021
43. Autonomic dysfunction, diabetes and metabolic syndrome
- Author
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Tae Yang Yu and Moon-Kyu Lee
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Metabolic Syndrome ,medicine.medical_specialty ,business.industry ,Endocrinology, Diabetes and Metabolism ,General Medicine ,RC648-665 ,medicine.disease ,Autonomic Nervous System ,Diseases of the endocrine glands. Clinical endocrinology ,Editorial ,Autonomic Nervous System Diseases ,Internal medicine ,Diabetes mellitus ,Internal Medicine ,medicine ,Diabetes Mellitus ,Humans ,Diabetic Nephropathies ,Metabolic syndrome ,business - Abstract
Relationships among autonomic nervous system, diabetes and metabolic syndrome.
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- 2021
44. Mechanical properties of four types of PVC-coated woven fabrics at high-temperature and after exposure to high-temperature
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Zhenggang Cao, Ying Sun, and Yang Yu
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Materials science ,Impact toughness ,0211 other engineering and technologies ,Uniaxial tension ,Stiffness ,020101 civil engineering ,02 engineering and technology ,Building and Construction ,Atmospheric temperature range ,0201 civil engineering ,Stress (mechanics) ,Polyvinyl chloride ,chemistry.chemical_compound ,chemistry ,Woven fabric ,021105 building & construction ,Architecture ,medicine ,Polymer physics ,medicine.symptom ,Composite material ,Safety, Risk, Reliability and Quality ,Civil and Structural Engineering - Abstract
In this paper, the mechanical properties of polyvinyl chloride (PVC)-coated woven fabric at high-temperature and after exposure to high-temperature were studied using a uniaxial tensile test. The test temperature range is 20 °C to 170 °C. The high-temperature test shows that the maximum stress at 170 °C reduced to 57%−59% of that at 20 °C. The after exposure to high-temperature test of prestressing shows that the combined effect of high-temperature and constant external force enhanced the stiffness and reduced the material’s impact toughness. The experimental phenomena can be explained by polymer physics.
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- 2021
45. Alterations in Effective Connectivity of the Hippocampus in Migraine without Aura
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Chunhong Hu, Hui Dai, Yang Yu, Jun Ke, Hongru Zhao, Ximing Wang, Yadi Zhu, Lingling Dai, and Boan Ji
- Subjects
medicine.diagnostic_test ,Resting state fMRI ,business.industry ,hippocampus ,effective connectivity ,Hippocampus ,medicine.disease ,functional magnetic resonance imaging ,Anesthesiology and Pain Medicine ,Chronic Migraine ,Migraine ,Neuroimaging ,nervous system ,resting-state ,medicine ,migraine ,Journal of Pain Research ,business ,Functional magnetic resonance imaging ,Prefrontal cortex ,Insula ,Neuroscience ,Original Research - Abstract
Yadi Zhu,1,2,* Lingling Dai,1,2,* Hongru Zhao,3,* Boan Ji,4 Yang Yu,1,2 Hui Dai,1,2 Chunhong Hu,1,2 Ximing Wang,1,2 Jun Ke1,2 1Department of Radiology, The First Affiliated Hospital of Soochow University, Soochow, Jiangsu Province, Peopleâs Republic of China; 2Institute of Medical Imaging, Soochow University, Soochow, Jiangsu Province, Peopleâs Republic of China; 3Department of Neurology, The First Affiliated Hospital of Soochow University, Soochow, Jiangsu Province, Peopleâs Republic of China; 4Medical School of Soochow University, Soochow, Jiangsu Province, Peopleâs Republic of China*These authors contributed equally to this workCorrespondence: Ximing Wang; Jun KeDepartment of Radiology, The First Affiliated Hospital of Soochow University, No. 188 Shizi Road, Soochow, 215000, Jiangsu Province, Peopleâs Republic of ChinaTel +86-512-67973062Fax +86-512-67973061Email wangximing1998@163.com; fmrikej@126.comPurpose: Neuroimaging studies on migraine have revealed structural and functional alterations in the hippocampus, a region involved in pain processing and stress response. This study was designed to investigate whether effective connectivity of this region is disrupted in migraine and relates to chronicity of this disease.Patients and Methods: In 39 episodic migraine (EM) patients, 17 chronic migraine (CM) patients, and 35 healthy controls, we investigated differences in the directional influences between the hippocampus and the rest of the brain by combining resting-state functional magnetic resonance imaging and Granger causality analysis (GCA), with bilateral hippocampus as seed regions. The associations between directional influences and the clinical variables were also examined.Results: Comparing each patient group to the control group, we found increased and decreased negative influence on the hippocampus exerted by the bilateral visual areas and right dorsolateral prefrontal cortex (dlPFC), respectively. The hippocampus showed increased positive influence on the right posterior insula and medial prefrontal cortex (mPFC), as well as increased negative influence on the left cerebellum in CM patients relative to EM patients and healthy controls. Furthermore, across all patients, the migraine frequency exhibited a positive and negative association with causal influence from the hippocampus to mPFC and left cerebellum, respectively.Conclusion: Migraine patients have abnormal effective connectivity between the hippocampus and multiple brain regions involved in the sensory and cognitive processing of pain. Disrupted directional influences to the hippocampus exerted by dlPFC and bilateral visual areas were common features of EM and CM patients. Directional influences from the hippocampus to mPFC and left cerebellum may be useful imaging biomarkers for assessing migraine frequency.Keywords: migraine, hippocampus, effective connectivity, resting-state, functional magnetic resonance imaging
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- 2021
46. Inflammatory markers in postoperative delirium (POD) and cognitive dysfunction (POCD): A meta-analysis of observational studies.
- Author
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Xuling Liu, Yang Yu, and Shengmei Zhu
- Subjects
Medicine ,Science - Abstract
BACKGROUND:The aim of this study was to summarize and discuss the similarities and differences in inflammatory biomarkers in postoperative delirium (POD) and cognitive dysfunction (POCD). METHODS:A systematic retrieval of literature up to June 2017 in PubMed, Embase, the Cochrane Library, the China National Knowledge Infrastructure database, and the Wanfang database was conducted. Extracted data were analyzed with STATA (version 14). The standardized mean difference (SMD) and the 95% confidence interval (95% CI) of each indicator were calculated using a random effect model. We also performed tests of heterogeneity, sensitivity analysis, assessments of bias, and meta-regression in this meta-analysis. RESULTS:A total of 54 observational studies were included. By meta-analysis we found significantly increased C-reactive protein (CRP) (9 studies, SMD 0.883, 95% CI 0.130 to 1.637, P = 0.022 in POD; 10 studies, SMD -0.133, 95% CI -0.512 to 0.246, P = 0.429 in POCD) and interleukin (IL)-6 (7 studies, SMD 0.386, 95% CI 0.054 to 0.717, P = 0.022 in POD; 16 studies, SMD 0.089, 95% CI -0.133 to 0.311, P = 0.433 in POCD) concentrations in both POD and POCD patients. We also found that the SMDs of CRP and IL-6 from POCD patients were positively correlated with surgery type in the meta-regression (CRP: Coefficient = 1.555365, P = 0.001, 10 studies; IL-6: Coefficient = -0.6455521, P = 0.086, 16 studies). CONCLUSION:Available evidence from medium-to-high quality observational studies suggests that POD and POCD are indeed correlated with the concentration of peripheral inflammatory markers. Some of these markers, such as CRP and IL-6, play roles in both POD and POCD, while others are specific to either one of them.
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- 2018
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47. Asymmetric polar localization dynamics of the serine chemoreceptor protein Tsr in Escherichia coli.
- Author
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Dongmyung Oh, Yang Yu, Hochan Lee, Jae-Hyung Jeon, Barry L Wanner, and Ken Ritchie
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Medicine ,Science - Abstract
The spatial location of proteins in living cells can be critical for their function. For example, the E. coli chemotaxis machinery is localized to the cell poles. Here we describe the polar localization of the serine chemoreceptor Tsr using a strain synthesizing a fluorescent Tsr-Venus fusion at a low level from a single-copy chromosomal construct. Using photobleaching and imaging during recovery by new synthesis, we observed distinct asymmetry between a bright (old) pole and a dim (new) pole. The old pole was shown to be a more stable cluster and to recover after photobleaching faster, which is consistent with the hypothesis that newly synthesized Tsr proteins are inserted directly at or near the old pole. The new pole was shown to be a less stable cluster and to exchange proteins freely with highly mobile Tsr-Venus proteins diffusing in the membrane. We propose that the new pole arises from molecules escaping from the old pole and diffusing to the new pole where a more stable cluster forms over time. Our localization imaging data support a model in which a nascent new pole forms prior to stable cluster formation.
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- 2018
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48. Synthesis and characterization of a magnetic adsorbent from negatively-valued iron mud for methylene blue adsorption.
- Author
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Jiancong Liu, Yang Yu, Suiyi Zhu, Jiakuan Yang, Jian Song, Wei Fan, Hongbin Yu, Dejun Bian, and Mingxin Huo
- Subjects
Medicine ,Science - Abstract
With increasing awareness of reduction of energy and CO2 footprint, more waste is considered recyclable for generating value-added products. Here we reported the negatively-valued iron mud, a waste from groundwater treatment plant, was successfully converted into magnetic adsorbent. Comparing with the conventional calcination method under the high temperature and pressure, the synthesis of the magnetic particles (MPs) by Fe2+/Fe3+ coprecipitation was conducted at environment-friendly condition using ascorbic acid (H2A) as reduction reagent and nitric acid (or acid wastewater) as leaching solution. The MPs with major component of Fe3O4 were synthesized at the molar ratio (called ratio subsequently) of H2A to Fe3+ of iron mud ≥ 0.1; while amorphous ferrihydrite phase was formed at the ratio ≤ 0.05, which were confirmed by vibrating sample magnetometer (VSM), X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS). With the ratio increased, the crystalline size and the crystallization degree of MPs increased, and thus the Brunauer-Emmett-Teller (BET) surface and the cation-exchange capacity (CEC) decreased. MPs-3 prepared with H2A to Fe3+ ratio of 0.1 demonstrated the highest methylene blue (MB) adsorption of 87.3 mg/g and good magnetic response. The adsorption of MB onto MPs agreed well with the non-linear Langmuir isotherm model and the pseudo-second-order model. Pilot-scale experiment showed that 99% of MB was removed by adding 10 g/L of MPs-3. After five adsorption-desorption cycles, MPs-3 still showed 62% removal efficiency for MB adsorption. When nitric acid was replaced by acid wastewater from a propylene plant, the synthesized MPs-3w showed 3.7 emu/g of saturation magnetization (Ms) and 56.7 mg/g of MB adsorption capacity, 2.8 times of the widely used commercial adsorbent of granular active carbon (GAC). The major mechanism of MPs adsorption for MB was electrostatic attraction and cation exchange. This study synthesized a magnetic adsorbent from the negatively-valued iron mud waste by using an environment-friendly coprecipitation method, which had a potential for treatment of dye wastewater.
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- 2018
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49. Correction: +mRNA expression of LRRC55 protein (leucine-rich repeat-containing protein 55) in the adult mouse brain.
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Ying-Ying Zhang, Xue Han, Ye Liu, Jian Chen, Lei Hua, Qian Ma, Yang-Yu-Xin Huang, Qiong-Yao Tang, and Zhe Zhang
- Subjects
Medicine ,Science - Abstract
[This corrects the article DOI: 10.1371/journal.pone.0191749.].
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- 2018
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50. Oxygenation in cell culture: Critical parameters for reproducibility are routinely not reported.
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Abdullah Al-Ani, Derek Toms, Douglas Kondro, Jarin Thundathil, Yang Yu, and Mark Ungrin
- Subjects
Medicine ,Science - Abstract
Mammalian cell culture is foundational to biomedical research, and the reproducibility of research findings across the sciences is drawing increasing attention. While many components contribute to reproducibility, the reporting of factors that impact oxygen delivery in the general biomedical literature has the potential for both significant impact, and immediate improvement. The relationship between the oxygen consumption rate of cells and the diffusive delivery of oxygen through the overlying medium layer means parameters such as medium depth and cell type can cause significant differences in oxygenation for cultures nominally maintained under the same conditions. While oxygenation levels are widely understood to significantly impact the phenotype of cultured cells in the abstract, in practise the importance of the above parameters does not appear to be well recognized in the non-specialist research community. On analyzing two hundred articles from high-impact journals we find a large majority missing at least one key piece of information necessary to ensure consistency in replication. We propose that explicitly reporting these values should be a requirement for publication.
- Published
- 2018
- Full Text
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