17 results on '"Cutter, Asher D."'
Search Results
2. Mainstreaming Caenorhabditis elegans in experimental evolution
- Author
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Gray, Jeremy C. and Cutter, Asher D.
- Published
- 2014
3. Molecular hyperdiversity defines populations of the nematode Caenorhabditis brenneri
- Author
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Dey, Alivia, Chan, Cecilia K. W., Thomas, Cristel G., and Cutter, Asher D.
- Published
- 2013
4. GENE DUPLICATION IN THE EVOLUTION OF SEXUAL DIMORPHISM
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Wyman, Minyoung J., Cutter, Asher D., and Rowe, Locke
- Published
- 2012
5. Males, Outcrossing, and Sexual Selection in Caenorhabditis Nematodes.
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Cutter, Asher D., Morran, Levi T., and Phillips, Patrick C.
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PHYSIOLOGICAL adaptation , *BIOLOGICAL evolution , *FEMALE reproductive organs , *MALE reproductive organs , *GENOMES , *NEMATODES , *PHENOTYPES - Abstract
Males of Caenorhabditis elegans provide a crucial practical tool in the laboratory, but, as the rarer and more finicky sex, have not enjoyed the same depth of research attention as hermaphrodites. Males, however, have attracted the attention of evolutionary biologists who are exploiting the C. elegans system to test longstanding hypotheses about sexual selection, sexual conflict, transitions in reproductive mode, and genome evolution, as well as to make new discoveries about Caenorhabditis organismal biology. Here, we review the evolutionary concepts and data informed by study of males of C. elegans and other Caenorhabditis. We give special attention to the important role of sperm cells as a mediator of inter-male competition and male-female conflict that has led to drastic trait divergence across species, despite exceptional phenotypic conservation in many other morphological features. We discuss the evolutionary forces important in the origins of reproductive mode transitions from males being common (gonochorism: females and males) to rare (androdioecy: hermaphrodites and males) and the factors that modulate male frequency in extant androdioecious populations, including the potential influence of selective interference, host-pathogen coevolution, and mutation accumulation. Further, we summarize the consequences of males being common vs rare for adaptation and for trait divergence, trait degradation, and trait dimorphism between the sexes, as well as for molecular evolution of the genome, at both micro-evolutionary and macro-evolutionary timescales.We conclude that C. elegans male biology remains underexploited and that future studies leveraging its extensive experimental resources are poised to discover novel biology and to inform profound questions about animal function and evolution. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
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6. Genome structure predicts modular transcriptome responses to genetic and environmental conditions.
- Author
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Mark, Stephanie, Weiss, Joerg, Sharma, Eesha, Liu, Ting, Wang, Wei, Claycomb, Julie M., and Cutter, Asher D.
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MODULAR construction ,GENE expression ,NATURAL selection ,CIS-regulatory elements (Genetics) ,GENOMES ,GENE regulatory networks ,PHYSIOLOGICAL effects of heat ,MOLECULAR evolution - Abstract
Understanding the plasticity, robustness and modularity of transcriptome expression to genetic and environmental conditions is crucial to deciphering how organisms adapt in nature. To test how genome architecture influences transcriptome profiles, we quantified expression responses for distinct temperature‐adapted genotypes of the nematode Caenorhabditis briggsae when exposed to chronic temperature stresses throughout development. We found that 56% of the 8,795 differentially expressed genes show genotype‐specific changes in expression in response to temperature (genotype‐by‐environment interactions, GxE). Most genotype‐specific responses occur under heat stress, indicating that cold vs. heat stress responses involve distinct genomic architectures. The 22 co‐expression modules that we identified differ in their enrichment of genes with genetic vs. environmental vs. interaction effects, as well as their genomic spatial distributions, functional attributes and rates of molecular evolution at the sequence level. Genes in modules enriched for simple effects of either genotype or temperature alone tend to evolve especially rapidly, consistent with disproportionate influence of adaptation or weaker constraint on these subsets of loci. Chromosome‐scale heterogeneity in nucleotide polymorphism, however, rather than the scale of individual genes predominates as the source of genetic differences among expression profiles, and natural selection regimes are largely decoupled between coding sequences and noncoding flanking sequences that contain cis‐regulatory elements. These results illustrate how the form of transcriptome modularity and genome structure contribute to predictable profiles of evolutionary change. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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7. When natural selection gives gene function the cold shoulder.
- Author
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Cutter, Asher D. and Jovelin, Richard
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NATURAL selection , *GENES , *EVOLUTION research , *BIOLOGICAL evolution , *GENETICS , *GENOMES - Abstract
It is tempting to invoke organismal selection as perpetually optimizing the function of any given gene. However, natural selection can drive genic functional change without improvement of biochemical activity, even to the extinction of gene activity. Detrimental mutations can creep in owing to linkage with other selectively favored loci. Selection can promote functional degradation, irrespective of genetic drift, when adaptation occurs by loss of gene function. Even stabilizing selection on a trait can lead to divergence of the underlying molecular constituents. Selfish genetic elements can also proliferate independent of any functional benefits to the host genome. Here we review the logic and evidence for these diverse processes acting in genome evolution. This collection of distinct evolutionary phenomena - while operating through easily understandable mechanisms - all contribute to the seemingly counterintuitive notion that maintenance or improvement of a gene's biochemical function sometimes do not determine its evolutionary fate. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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8. MicroRNA Sequence Variation Potentially Contributes to Within-Species Functional Divergence in the Nematode Caenorhabditis briggsae.
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Jovelin, Richard and Cutter, Asher D.
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NUCLEOTIDE sequence , *CAENORHABDITIS , *ARABIDOPSIS thaliana , *BIOLOGICAL variation , *GENOMES , *NEMATODES - Abstract
Mounting evidence points to differences in gene regulation as a major source of phenotypic variation. MicroRNA-mediated post-transcriptional regulation has emerged recently as a key factor controlling gene activity during development. MicroRNA genes are abundant in genomes, acting as managers of gene expression by directing translational repression. Thus, understanding the role of microRNA sequence variation within populations is essential for fully dissecting the origin and maintenance of phenotypic diversity in nature. In this study, we investigate allelic variation at microRNA loci in the nematode Caenorhabditis briggsae, a close relative of C. elegans. Phylogeographic structure in C. briggsae partitions most strains from around the globe into a "temperate" or a "tropical" clade, with a few strains having divergent, geographically restricted genotypes. Remarkably, strains that follow this latitudinal dichotomy also differ in temperature-associated fitness. With this phylogeographic pattern in mind, we examined polymorphisms in 18 miRNAs in a global sample of C. briggsae isolates and tested whether newly isolated strains conform to this phylogeography. Surprisingly, nucleotide diversity is relatively high in this class of gene that generally experiences strong purifying selection. In particular, we find that miRNAs in C. briggsae are substantially more polymorphic than in Arabidopsis thaliana, despite similar background levels of neutral site diversity between the two species. We find that some mutations suggest functional divergence on the basis of requirements for target site recognition and computational prediction of the effects of the polymorphisms on RNA folding. These findings demonstrate the potential for miRNA polymorphisms to contribute to phenotypic variation within a species. Sequences were deposited in GenBank under accession nos. JN251323--JN251744. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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9. The Evolutionary Dynamics of Operon Distributions in Eukaryote Genomes.
- Author
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Cutter, Asher D. and Agrawal, Aneil F.
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GENOMES , *GENETIC regulation , *BIOLOGICAL evolution , *GENETIC transcription , *GENE expression - Abstract
Genes in nematode and ascidian genomes frequently occur in operons-multiple genes sharing a common promoter to generate a polycistronic primary transcript-and such genes comprise 15-20% of the coding genome for Caenorhabditis elegans and Ciona intestinalis. Recent work in nematodes has demonstrated that the identity of genes within operons is highly conserved among species and that the unifying feature of genes within operons is that they are expressed in germline tissue. However, it is generally unknown what processes are responsible for generating the distribution of operon sizes across the genome, which are composed of up to eight genes per operon. Here we investigate several models for operon evolution to better understand their abundance, distribution of sizes, and evolutionary dynamics over time.We find that birth-death models of operon evolution reasonably describe the relative abundance of operons of different sizes in the C. elegans and Ciona genomes and generate predictions about the number of monocistronic, nonoperon genes that likely participate in the birth-death process. This theory, and applications to C. elegans and Ciona, motivates several new and testable hypotheses about eukaryote operon evolution. [ABSTRACT FROM AUTHOR]
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- 2010
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10. Germline Expression Influences Operon Organization in the Gaenorhabditis elegans Genome.
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Reinke, Valerie and Cutter, Asher D.
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GERM cells , *HEREDITY , *GENE expression , *GENETICS , *IN situ hybridization , *GENES , *GENOMES - Abstract
Operons are found across multiple kingdoms and phyla, from prokaryotes to chordates. In the nematode Caenor/iabditis elegans, the genome contains >1000 operons that compose ∼15% of the protein-coding genes. However, determination of the force(s) promoting the origin and maintenance of operons in C. elegans has proved elusive. Compared to bacterial operons, genes within a C. elegans operon often show poor coexpression and only sometimes encode proteins with related functions. Using analysis of microarray and large-scale in situ hybridization data, we demonstrate that almost all operon-encoded genes are expressed in germline tissue. However, genes expressed during spermatogenesis are excluded from operons. Operons group together along chromosomes in local clusters that also contain monocistronic germline-expressed genes. Additionally, germline expression of genes in operons is largely independent of the molecular function of the encoded proteins. These analyses demonstrate that mechanisms governing germline gene expression influence operon origination and/or maintenance. Thus, gene expression in a specific tissue can have profound effects on the evolution of genome organization. [ABSTRACT FROM AUTHOR]
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- 2009
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11. Patterns of Molecular Evolution in Caenorhabditis Preclude Ancient Origins of Selfing.
- Author
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Cutter, Asher D., Wasmuth, James D., and Washingtont, Nicole L.
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GENOMES , *GENOMICS , *NUCLEOTIDES , *CAENORHABDITIS elegans , *MOLECULAR biology , *GENETICS - Abstract
The evolution of self-fertilization can mediate pronounced changes in genomes as a by-product of a drastic reduction in effective population size and the concomitant accumulation of slightly deleterious mutations by genetic drift. In the nematode genus Caenorhabditis, a highly selfing lifestyle has evolved twice independently, thus permitting an opportunity to test for the effects of mode of reproduction on patterns of molecular evolution on a genomic scale. Here we contrast rates of nucleotide substitution and codon usage bias among thousands of orthologous groups of genes in six species of Caenorhabditis, including the classic model organism Caenorhabditis elegans. Despite evidence that weak selection on synonymous codon usage is pervasive in the history of all species in this genus, we find little difference among species in the patterns of codon usage bias and in replacement-site substitution. Applying a model of relaxed selection on codon usage to the C. elegans and C. bcriggsae lineages suggests that self-fertilization is unlikely to have evolved more than `.. 4 million years ago, which is less than a quarter of the time since they shared a common ancestor with outcrossing species. We conclude that the profound changes in mating behavior, physiology, and developmental mechanisms that accompanied the transition from an obligately outcrossing to a primarily selfing mode of reproduction evolved in the not-too-distant past. [ABSTRACT FROM AUTHOR]
- Published
- 2008
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12. Patterns of Nucleotide Polymorphism Distinguish Temperate and Tropical Wild Isolates of Caenorhabditis briggsae.
- Author
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Cutter, Asher D., Félix, Marie-Anne, Barriëre, Antoine, and Charlesworth, Deborah
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CAENORHABDITIS , *NEMATODES , *GENOMES , *GENETIC polymorphisms , *GENETICS - Abstract
Caenorhabditis briggsae provides a natural comparison species for the model nematode C. elegans, given their similar morphology, life history, and hermaphroditic mode of reproduction. Despite C. briggsae boasting a published genome sequence and establishing Caenorhabditis as a model genus for genetics and development, little is known about genetic variation across the geographic range of this species. In this study, we greatly expand the collection of natural isolates and characterize patterns of nucleotide variation for six loci in 63 strains from three continents. The pattern of polymorphisms reveals differentiation between C. briggsae strains found in temperate localities in the northern hemisphere from those sampled near the Tropic of Cancer, with diversity within the tropical region comparable to what is found for C. elegans in Europe. As in C. elegans, linkage disequilibrium is pervasive, although recombination is evident among some variant sites, indicating that outcrossing has occurred at a low rate in the history of the sample. In contrast to C. elegans, temperate regions harbor extremely little variation, perhaps reflecting colonization and recent expansion of C. briggsae into northern latitudes. We discuss these findings in relation to their implications for selection, demographic history, and the persistence of self-fertilization. [ABSTRACT FROM AUTHOR]
- Published
- 2006
- Full Text
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13. Nucleotide Polymorphism and Linkage Disequilibrium in Wild Populations of the Partial Selfer Caenorhabditis elegans.
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Cutter, Asher D.
- Subjects
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HUMAN genetic variation , *NUCLEOTIDES , *GENETIC polymorphisms , *CAENORHABDITIS elegans , *GENOMES , *CHROMOSOMES , *GENETICS - Abstract
An understanding of the relative contributions of different evolutionary forces on an organism's genome requires an accurate description of the patterns of genetic variation within and between natural populations. To this end, I report a survey of nucleotide polymorphism in six loci from 118 strains of the nematode Caenorhabditis elegans. These strains derive from wild populations of several regions within France, Germany, and new localities in Scotland, in addition to stock center isolates. Overall levels of silent-site diversity are low within and between populations of this self-fertile species, averaging 0.2% in European samples and 0.3% worldwide. Population structure is present despite a lack of association of sequences with geography, and migration appears to occur at all geographic scales. Linkage disequilibrium is extensive in the C. elegans genome, extending even between chromosomes. Nevertheless, recombination is clearly present in the pattern of polymorphisms, indicating that outcrossing is an infrequent, but important, feature in this species ancestry. The range of outcrossing rates consistent with the data is inferred from linkage disequilibrium, using ‘scattered’ samples representing the collecting phase of the coalescent process in a subdivided population. I propose that genetic variation in this species is shaped largely by population subdivision due to serf-fertilization coupled with long- and short-range migration between subpopulations. [ABSTRACT FROM AUTHOR]
- Published
- 2006
- Full Text
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14. Transposable Element Orientation Bias in the Drosophila melanogaster Genome.
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Cutter, Asher D., Good, Jeffrey M., Pappas, Christopher T., Saunders, Matthew A., Starrett, Dean M., and Wheeler, Travis J.
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DROSOPHILA melanogaster , *GENETIC transcription , *GENOMES , *GENETICS , *GENES , *RNA , *DNA , *DROSOPHILA - Abstract
Nonrandom distributions of transposable elements can be generated by a variety of genomic features. Using the full D. melanogaster genome as a model, we characterize the orientations of different classes of transposable elements in relation to the directionality of genes. DNA-mediated transposable elements are more likely to be in the same orientation as neighboring genes when they occur in the nontranscribed region’s that flank genes. However, RNA-mediated transposable elements located in an intron are more often oriented in the direction opposite to that of the host gene. These orientation biases are strongest for genes with highly biased codon usage, probably reflecting the ability of such loci to respond to weak positive or negative selection. The leading hypothesis for selection against transposable elements in the coding orientation proposes that transcription termination poly(A) signal motifs within retroelements interfere with normal gene transcription. However, after accounting for differences in base composition between the strands, we find no evidence for global selection against spurious transcription termination signals in introns. We therefore conclude that premature termination of host gene transcription due to the presence of poly(A) signal motifs in retroelements might only partially explain strand-specific detrimental effects in the D. melanogaster genome. [ABSTRACT FROM AUTHOR]
- Published
- 2005
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15. Clustered Organization of Reproductive Genes in the C. elegans Genome
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Miller, Michael A., Cutter, Asher D., Yamamoto, Ikuko, Ward, Samuel, and Greenstein, David
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CAENORHABDITIS elegans , *GENOMES , *CAENORHABDITIS , *GENETICS - Abstract
Defining the forces that sculpt genome organization is fundamental for understanding the origin, persistence, and diversification of species . The genomic sequences of the nematodes Caenorhabditis elegans and Caenorhabditis briggsae provide an excellent opportunity to explore the dynamics of chromosome evolution . Extensive chromosomal rearrangement has accompanied divergence from their common ancestor, an event occurring roughly 100 million years ago (Mya) ; yet, morphologically, these species are nearly indistinguishable and both reproduce primarily by self-fertilization. Here, we show that genes expressed during spermatogenesis (sperm genes) are nonrandomly distributed across the C. elegans genome into three large clusters located on two autosomes. In addition to sperm genes, these chromosomal regions are enriched for genes involved in the hermaphrodite sperm/oocyte switch and in the reception of sperm signals that control fertilization. Most loci are present in single copy, suggesting that cluster formation is largely due to gene aggregation and not to tandem duplication. Comparative mapping indicates that the C. briggsae genome differs dramatically from the C. elegans genome in clustering. Because clustered genes have a direct role in reproduction and thus fitness, their aggregated pattern might have been shaped by natural selection, perhaps as hermaphroditism evolved. [Copyright &y& Elsevier]
- Published
- 2004
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16. Correction: Reproductive Mode and the Evolution of Genome Size and Structure in Caenorhabditis Nematodes.
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Fierst, Janna L., Willis, John H., Thomas, Cristel G., Wang, Wei, Reynolds, Rose M., Ahearne, Timothy E., Cutter, Asher D., and Phillips, Patrick C.
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GENOMES ,NEMATODES - Abstract
A correction to the article "Reproductive Mode and the Evolution of Genome Size and Structure in Caenorhabditis Nematodes" is presented.
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- 2015
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17. The distribution of mutational effects on fitness in Caenorhabditis elegans inferred from standing genetic variation.
- Author
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Gilbert, Kimberly J., Zdraljevic, Stefan, Cook, Daniel E., Cutter, Asher D., Andersen, Erik C., and Baer, Charles F.
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GENETIC mutation , *GENETICS , *CAENORHABDITIS elegans , *ANIMAL experimentation , *STANDING position , *GENETIC variation , *GENOMES - Abstract
The distribution of fitness effects (DFE) for new mutations is one of the most theoretically important but difficult to estimate properties in population genetics. A crucial challenge to inferring the DFE from natural genetic variation is the sensitivity of the site frequency spectrum to factors like population size change, population substructure, genome structure, and nonrandom mating. Although inference methods aim to control for population size changes, the influence of nonrandom mating remains incompletely understood, despite being a common feature of many species. We report the DFE estimated from 326 genomes of Caenorhabditis elegans, a nematode roundworm with a high rate of self-fertilization. We evaluate the robustness of DFE inferences using simulated data that mimics the genomic structure and reproductive life history of C. elegans. Our observations demonstrate how the combined influence of self-fertilization, genome structure, and natural selection on linked sites can conspire to compromise estimates of the DFE from extant polymorphisms with existing methods. These factors together tend to bias inferences toward weakly deleterious mutations, making it challenging to have full confidence in the inferred DFE of new mutations as deduced from standing genetic variation in species like C. elegans. Improved methods for inferring the DFE are needed to appropriately handle strong linked selection and selfing. These results highlight the importance of understanding the combined effects of processes that can bias our interpretations of evolution in natural populations. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
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