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In this study, we determined the complete mitochondrial genome of the invasive insect species Melanoplus differentialis captured in Korea. The complete mitochondrial genome of M. differentialis is 15,625 bp long and comprises 13 protein‐coding genes, two ribosomal RNA genes, and 22 transfer RNAs, with a GC ratio of 25.2%. In total, 353 SNPs and 11 INDEL regions (total length 67 bp) were found against the previously sequenced M. differentialis mitochondrial genome recorded as public genome data. The number of interspecific variations was greater than the number of intraspecific variations in this insect. Phylogenetic tree analysis showed that the mitochondrial genome clustered the Melanoplus clade with two previously reported Melanoplus sequences. However, the sequences were not divided at the species‐level clade possibly as a consequence of misidentification caused by an error in the public database. Our results extend the molecular database status of Melanoplus by providing a novel complete mitochondrial genome sequence for M. differentialis that could serve as reference for further molecular studies. [ABSTRACT FROM AUTHOR]

Decapod hepandensovirus 1 (DHPV) is a viral pathogen that causes growth retardation and decreased feed conversion efficiency in penaeid shrimp. The virus exhibits high genetic variation between different hosts and geographical locations. It has been primarily reported in cultured Penaeus monodon , P. chinensis , and P. merguiensis , and a new type of DHPV has recently been reported in P. vannamei cultured in Korea and Taiwan. In this study, the genome of a recently reported new type of DHPV was fully sequenced by conventional Sanger sequencing combined with a next-generation sequencing approach. Similar to the other types of DHPVs infecting several shrimp species other than P. vannamei , a new type of DHPV contained a linear, single-stranded DNA genome of 6173 bp comprising three major open reading frames. However, by detailed comparative genome and phylogenetic analysis, the virus was not clustered with the pre-existing genotypes, suggesting the emergence of a possible novel genotype of DHPV in cultured shrimps. This study is the first to report the complete genomic sequence of DHPV identified in P. vannamei. • A new genotype of Decapod hepandensovirus 1 (DHPV) is found in Penaeus vannamei. • Complete genome sequence of DHPV infecting Penaeus vannamei presented in the study. • The DNA genome is 6173 bp in size, comprising 3 major open reading frames (ORFs). • ORF 3 region of the new DHPV shows notably low similarity to 9 historic viruses. • The phylogenetic tree shows the new DHPV doesn't cluster with existing genotypes. [ABSTRACT FROM AUTHOR]

Arion ater are a group of mollusks, almost all of which live in a dark and humid environment during their life cycle. In this study, the complete mitogenome of Arion ater was sequenced using next generation sequencing, analyzed and compared with other Stylommatophorans. The total length of the mitochondrial genome is 14,314 bp and consists of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and 1 control region. The genome has a typical mitochondrial gene sequence of Arion. Phylogenetic analysis of the mitochondrial genomes of 27 species of Stylommatophora showed that the Arion ater, Arion rufus and Arion vulgaris forms a monophyletic group with other species. [ABSTRACT FROM AUTHOR]

Kosta goat is one of the Indonesian local goats which had the potential to utilize, while their genetics was not yet characterized. Since, a high mutation of Mitochondria DNA (mtDNA), it is widely applied to understand species evolutionary, phylogenetic, and characterized population genetics. Whole genome sequencing of Mitochondria DNA has new insight to elucidate genetic characteristic of goat completely. Therefore, this study purposes to explore the whole mitochondrial DNA of the Kosta goat and its relationship with other breeds based on Whole Genome Sequensing (WGS). The Kosta goat mitochondria genome was collected from whole blood, which was enriched using REPLI-g Mitochondrial DNA Kit (Qiagen). Oxford Nanopore Technology was employed in the mtDNA sequencing. The assembled sequence was polished with Racon and Medaka , and was further determined using Quast. Furthermore, Annotation and Visualization were conducted using MitoZ and Mitos Webserver. The sequence mitochondria genome of the Kosta goat has 16,641 bp, consisting of 13 PCGs (Protein Coding Genes), 22 tRNA, 2 rRNA, D_loop region, as well as OL (origin loop) sequences. Also, it has a base composition of 33.57 % for Adenin (A), 27.31 % for Timin (T), 26.04 % for Cytosin (C), and 13.08 % for Guanin (G), with sequential percentages of A>T>C>G. The start codons of PGCs are primarily initiated by ATG, whereas for ND2, ND3, and ND5 by ATA. Specific genetic characteristic of mitogenome Kosta goat has 309 bp length of ND3. Kosta goat belongs to Lineage B, and this follows the study of other Indonesian goats. Complete mitochondrial DNA of Kosta goats can be used to characterize their genetic potential, genetic diversity, phylogenetic analysis to support their utilization and conservation. • Kosta goat is one of Indonesian local goats. • Complete Mitochondrial DNA sequencing is a new insight to elucidate genetic characteristic of Kosta goats. • Complete mitochondrial DNA of Kosta goat may help provide data for further analysis on the mtDNA sequence to support goat genetic research. [ABSTRACT FROM AUTHOR]

The Melanoides tuberculata is an invasive species, which is natively distributed in Africa and Southeast Asia. This study describes the first mitochondrial genome of the M. tuberculata based on the whole genome sequencing data. The complete sequence length of the mitogenome is 15,821 bp, including 37 genes (2 rRNA genes, 22 tRNA genes and 13 protein-coding genes). Phylogenetic analysis using the 13 species of Cerithioidea species showed that the M. tuberculata is closely related to P. dartevellei, forming the sister group to C. sinensis and C. obtuse. [ABSTRACT FROM AUTHOR]