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Though artificial intelligence (AI) has been used in nuclear medicine for more than 50 years, more progress has been made in deep learning (DL) and machine learning (ML), which have driven the development of new AI abilities in the field. ANNs are used in both deep learning and machine learning in nuclear medicine. Alternatively, if 3D convolutional neural network (CNN) is used, the inputs may be the actual images that are being analyzed, rather than a set of inputs. In nuclear medicine, artificial intelligence reimagines and reengineers the field's therapeutic and scientific capabilities. Understanding the concepts of 3D CNN and U-Net in the context of nuclear medicine provides for a deeper engagement with clinical and research applications, as well as the ability to troubleshoot problems when they emerge. Business analytics, risk assessment, quality assurance, and basic classifications are all examples of simple ML applications. General nuclear medicine, SPECT, PET, MRI, and CT may benefit from more advanced DL applications for classification, detection, localization, segmentation, quantification, and radiomic feature extraction utilizing 3D CNNs. An ANN may be used to analyze a small dataset at the same time as traditional statistical methods, as well as bigger datasets. Nuclear medicine's clinical and research practices have been largely unaffected by the introduction of artificial intelligence (AI). Clinical and research landscapes have been fundamentally altered by the advent of 3D CNN and U-Net applications. Nuclear medicine professionals must now have at least an elementary understanding of AI principles such as neural networks (ANNs) and convolutional neural networks (CNNs)., Competing Interests: The authors declared that they have no conflicts of interest regarding this work., (Copyright © 2022 P. Manimegalai et al.)