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Your search keyword '"Xu, Jieting"' showing total 2 results
Start Over Author "Xu, Jieting" Topic crispr-associated protein 9 Publication Year Range Last 50 years
2 results on '"Xu, Jieting"'

1. High-Throughput CRISPR/Cas9 Mutagenesis Streamlines Trait Gene Identification in Maize.

Author

Liu HJ, Jian L, Xu J, Zhang Q, Zhang M, Jin M, Peng Y, Yan J, Han B, Liu J, Gao F, Liu X, Huang L, Wei W, Ding Y, Yang X, Li Z, Zhang M, Sun J, Bai M, Song W, Chen H, Sun X, Li W, Lu Y, Liu Y, Zhao J, Qian Y, Jackson D, Fernie AR, and Yan J

Subjects
Base Sequence, DNA Repair genetics, Gene Editing, Mutation genetics, Plants, Genetically Modified, Plasmids genetics, RNA, Guide, CRISPR-Cas Systems genetics, Reproducibility of Results, Templates, Genetic, Transformation, Genetic, CRISPR-Associated Protein 9 metabolism, CRISPR-Cas Systems genetics, Genes, Plant, Mutagenesis genetics, Quantitative Trait, Heritable, Zea mays genetics
Abstract

Maize ( Zea mays ) is one of the most important crops in the world. However, few agronomically important maize genes have been cloned and used for trait improvement, due to its complex genome and genetic architecture. Here, we integrated multiplexed CRISPR/Cas9-based high-throughput targeted mutagenesis with genetic mapping and genomic approaches to successfully target 743 candidate genes corresponding to traits relevant for agronomy and nutrition. After low-cost barcode-based deep sequencing, 412 edited sequences covering 118 genes were precisely identified from individuals showing clear phenotypic changes. The profiles of the associated gene-editing events were similar to those identified in human cell lines and consequently are predictable using an existing algorithm originally designed for human studies. We observed unexpected but frequent homology-directed repair through endogenous templates that was likely caused by spatial contact between distinct chromosomes. Based on the characterization and interpretation of gene function from several examples, we demonstrate that the integration of forward and reverse genetics via a targeted mutagenesis library promises rapid validation of important agronomic genes for crops with complex genomes. Beyond specific findings, this study also guides further optimization of high-throughput CRISPR experiments in plants., (© 2020 American Society of Plant Biologists. All rights reserved.)

Published
2020
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2. High-Throughput CRISPR/Cas9 Mutagenesis Streamlines Trait Gene Identification in Maize

Author

Xi’ang Sun, Jianbing Yan, Hanmo Chen, Jiuran Zhao, Qinghua Zhang, Wenhao Song, David A. Jackson, Minliang Jin, Yangwen Qian, Yong Peng, Yuming Lu, Liu Xiangguo, Liumei Jian, Jiamin Sun, Han Baozhu, Jiali Yan, Alisdair R. Fernie, Zhang Maolin, Minji Bai, Xu Jieting, Wenqiang Li, Ya Liu, Mingliang Zhang, Fan Gao, Haijun Liu, Wenjie Wei, Yang Xiaofeng, Jie Liu, Huang Lei, Yunxiu Ding, and Zhenxian Li

Subjects
0106 biological sciences, 0301 basic medicine, Candidate gene, DNA Repair, Plant Science, Computational biology, Quantitative trait locus, Biology, Genes, Plant, Zea mays, 01 natural sciences, Genome, In Brief, 03 medical and health sciences, Quantitative Trait, Heritable, Transformation, Genetic, Genome editing, CRISPR-Associated Protein 9, CRISPR, Gene Editing, Base Sequence, Cas9, Reproducibility of Results, Templates, Genetic, Cell Biology, Plants, Genetically Modified, Reverse genetics, Genetic architecture, 030104 developmental biology, Mutagenesis, Mutation, CRISPR-Cas Systems, Plasmids, RNA, Guide, Kinetoplastida, 010606 plant biology & botany
Abstract

Maize (Zea mays) is one of the most important crops in the world. However, few agronomically important maize genes have been cloned and used for trait improvement, due to its complex genome and genetic architecture. Here, we integrated multiplexed CRISPR/Cas9-based high-throughput targeted mutagenesis with genetic mapping and genomic approaches to successfully target 743 candidate genes corresponding to traits relevant for agronomy and nutrition. After low-cost barcode-based deep sequencing, 412 edited sequences covering 118 genes were precisely identified from individuals showing clear phenotypic changes. The profiles of the associated gene-editing events were similar to those identified in human cell lines and consequently are predictable using an existing algorithm originally designed for human studies. We observed unexpected but frequent homology-directed repair through endogenous templates that was likely caused by spatial contact between distinct chromosomes. Based on the characterization and interpretation of gene function from several examples, we demonstrate that the integration of forward and reverse genetics via a targeted mutagenesis library promises rapid validation of important agronomic genes for crops with complex genomes. Beyond specific findings, this study also guides further optimization of high-throughput CRISPR experiments in plants.

Published
2020

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