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Summary: Monoterpenes are the most prevalent compounds found in essential oils. They exhibit inhibitory actions against phytopathogenic postharvest fungi. Direct application limits their effectiveness due to their instability, high volatility, hydrophobicity and susceptibility to degradation. Encapsulation systems using metal–organic frameworks (MOFs) have been developed to maximise their use. In this study, four protocols were shown to encapsulate thymol and limonene in porous solids, such as ZIF‐8, UiO‐66‐(COOH)2 and zeolite 13X. Vapourisation and diffusion at 60 °C for 16 h was found to be the most efficient encapsulation process. A larger amount of thymol than limonene was loaded in the porous solids. Thymol released from MOFs slowed down the growth of Colletotrichum musae by up to 6 days at 25 °C. There was a sustained release of thymol even beyond the period of maximum fungal growth. This proof‐of‐concept study revealed the potential utility of MOFs as carriers of thymol against postharvest fungi. [ABSTRACT FROM AUTHOR]

Summary: Bananas are tropical fruits that are perishable and susceptible to fungal diseases, mainly caused by Colletotrichum musae. Antimicrobial emulsion‐based coating materials have been used extensively to inhibit the growth of these fungi and extend the shelf life of bananas. Targeted emulsion‐based film functionalities offer excellent mechanical, physicochemical and barrier properties and limit fungal growth kinetics. Antifungal compounds, such as essential oils or phenolic compounds, are added to emulsion‐based (wax/biopolymer) coatings to enhance their antimicrobial properties. This study set out to formulate a beeswax‐based emulsion for banana coatings using high‐pressure homogenisation (HPH) and hydrophilic food‐grade/organic coating materials. The particle size distribution, stability and mechanical properties of the film‐forming emulsions, water vapour and the oxygen permeabilities of the resulting coating were investigated. The most promising sucrose emulsions were tested as coatings on banana fruits to assess their efficacy against moisture and firmness loss during storage. Adding thymol (0.5% w/w) to the sucrose emulsions further enhanced the inhibition of Colletotrichum musae growth to its barest minimum of <20%. Emulsion stability was clearly enhanced using HPH while the best inhibition was obtained with a 20% diluted sucrose emulsion containing 0.5% thymol, with or without HPH. [ABSTRACT FROM AUTHOR]

A semi-empirical model was developed to predict simultaneously time-temperature distribution inside the unripe banana and fungal reduction during the hot water dip, in order to simulate different process conditions for controlling banana anthracnose disease. For this purpose, the kinetic parameters of Colletotrichum musae reduction were estimated based on the thermal death time concept (z = 3.56 °C and D 50 ° C = 1044 s), and evaluated in different simulated media (unripe and fully ripe banana peel agar media). At the same time, a heat transfer model based on the Fourier equation was developed considering two unripe banana domains (peel and pulp). The comparison between the predicted and observed number of C. musae spores after the thermal treatment of unripe banana was satisfactory (coefficient of variation less than 10%), suggesting that the survival ratio is affected by the banana composition. The resulting model can be used to simulate different time-temperature scenarios, and to show how the parameter combinations may affect C. musae reduction in the unripe banana during the hot water dip process. • Identification of the kinetic parameters of Colletotrichum musae inactivation. • Development and validation of banana heat transfer model with two domains. • Validation of the fungal reduction in unripe banana under different scenarios. • Potential of numerical modeling for evaluating decontamination of other fruit. [ABSTRACT FROM AUTHOR]

• 12 isoflavonoid compounds identified out of 38 detected of EERBP by HPLC-ESI/MS. • Inhibition (81%) on mycelial growth of C. musae by EERBP (1.6 g GAE L−1). • Two fractions of EERBP detected and isolated by TLC-bioautography method. • An additive effect observed through the biological activity of these 2 fractions. This study set out to highlight the in vitro and in vivo antifungal activity of an Ethanolic Extract of Red Brazilian Propolis (EERBP) and identify bioactive fractions effective against Colletotrichum musae. Active fractions were detected by the thin-layer chromatography-bioautography method and characterised by HPLC-MS n. The in vitro results showed that EERBP had strong antifungal properties against C. musae (81 ± 1% inhibition at 1.6 g GAE L−1). Medicarpin, (3 S)-vestitol and (3 S)-neovestitol were the main compounds identified in the EERBP extract (45% of all detected peaks). Two isolated fractions displayed inhibition percentages of 35 ± 4 and 42 ± 1%, respectively, on C. musae mycelial growth compared to the EERBP extract. The biological activity of the two fractions displayed an additive effect. A further in vivo investigation revealed that EERBP is a potential natural alternative for controlling banana crown rot. [ABSTRACT FROM AUTHOR]