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The aim of this study is to sense the non-diabetic and diabetic blood using silver nanoparticles (SNP) by nucleation and growth method in novel analytical analysis in nanotechnology. The nucleation and growth approach was utilized to make SNP. The groups were analyzed with the same sample size of 26 for each group and the total sample size is 52 with G power of 80%, and confidence interval of 95%. SEM and UV Visible spectroscopy was used to characterize nanoparticles. The absorbance and morphology of the samples is analyzed. Different absorbances in different wavelengths were evident in nanoparticles and is very essential to analyze the absorbance of the nanoparticles. The particles were analyzed from 200-700 nm. Sensing of diabetic blood with nanoparticles have high absorbance in the visible region of 440-490 nm in blue region whereas for non diabetic blood with silver nanoparticles, it is noted to have high absorbance in the visible region ranging about 200-400 nm in UV region. Morphological characteristics was analyzed using SEM to determine the size distribution of silver nanoparticles. The morphology (SEM) is taken to analyze the size distribution of the particle. The absorbance of non diabetic blood with nanoparticles has a statistically insignificant value of 0.227 (p>0.05) for 60mM concentration. Conclusion: The non-diabetic blood and diabetic blood is sensed using silver nanoparticles by nucleation and growth methods. Both of these capping agents are found to be less toxic which is not very suitable for antibacterial activity. So the synthesis of silver nanoparticles is suggested to be put forward in in-vivo analysis. The nanoparticles can be used to diagnose live cells. [ABSTRACT FROM AUTHOR]