22 results on '"Pereira, Sónia O."'
Search Results
2. Cortisol AuPd plasmonic unclad POF biosensor
- Author
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Leitão, Cátia, Leal-Junior, Arnaldo, Almeida, Ana R., Pereira, Sónia O., Costa, Florinda M., Pinto, João L., and Marques, C.
- Published
- 2021
- Full Text
- View/download PDF
3. Impact of critical micelle concentration of macroRAFT agents on the encapsulation of colloidal Au nanoparticles
- Author
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Pereira, Sónia O., Trindade, Tito, and Barros-Timmons, Ana
- Published
- 2019
- Full Text
- View/download PDF
4. Insights into the photoluminescence properties of gel-like carbon quantum dots embedded in poly(methyl methacrylate) polymer
- Author
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Rodrigues, Joana, Pereira, Sónia O., Soreto Teixeira, S., Zhou, Yiqun, Peng, Zhili, Liyanage, Piumi Y., Leblanc, Roger M., Barros-Timmons, Ana M.M.V., Costa, Luis C., Costa, Florinda M., and Monteiro, Teresa
- Published
- 2019
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5. Detection of NT-proBNP Using Optical Fiber Back-Reflection Plasmonic Biosensors.
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Assunção, Ana Sofia, Vidal, Miguel, Martins, Maria João, Girão, Ana Violeta, Loyez, Médéric, Caucheteur, Christophe, Mesquita-Bastos, José, Costa, Florinda M., Pereira, Sónia O., and Leitão, Cátia
- Subjects
BIOSENSORS ,PLASMONICS ,NATRIURETIC peptides ,POINT-of-care testing ,PEPTIDES - Abstract
Heart failure (HF) is a clinical entity included in cardiovascular diseases affecting millions of people worldwide, being a leading cause of hospitalization of older adults, and therefore imposing a substantial economic burden on healthcare systems. HF is characterized by dyspnea, fatigue, and edema associated with elevated blood levels of natriuretic peptides, such as N Terminal pro-B-type Natriuretic Peptide (NT-proBNP), for which there is a high demand for point of care testing (POCT) devices. Optical fiber (OF) biosensors offer a promising solution, capable of real-time detection, quantification, and monitoring of NT-proBNP concentrations in serum, saliva, or urine. In this study, immunosensors based on plasmonic uncladded OF tips were developed using OF with different core diameters (200 and 600 µm). The tips were characterized to bulk refractive index (RI), anddetection tests were conducted with NT-proBNP concentrations varying from 0.01 to 100 ng/mL. The 200 µm sensors showed an average total variation of 3.6 ± 2.5 mRIU, an average sensitivity of 50.5 mRIU/ng·mL
−1 , and a limit of detection (LOD) of 0.15 ng/mL, while the 600 µm sensors had a response of 6.1 ± 4.2 mRIU, a sensitivity of 102.8 mRIU/ng·mL−1 , and an LOD of 0.11 ng/mL. Control tests were performed using interferents such as uric acid, glucose, and creatinine. The results show the potential of these sensors for their use in biological fluids. [ABSTRACT FROM AUTHOR]- Published
- 2024
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- View/download PDF
6. Biotinylation of optically responsive gold/polyelectrolyte nanostructures
- Author
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Pereira, Sónia O., Trindade, Tito, and Barros-Timmons, Ana
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- 2015
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7. Biofunctionalisation of colloidal gold nanoparticles via polyelectrolytes assemblies
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Pereira, Sónia O., Barros-Timmons, Ana, and Trindade, Tito
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- 2014
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8. Laser-induced graphene from paper for non-enzymatic uric acid electrochemical sensing in urine.
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Kulyk, Bohdan, Pereira, Sónia O., Fernandes, António J.S., Fortunato, Elvira, Costa, Florinda M., and Santos, Nuno F.
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URIC acid , *URINE , *GRAPHENE , *VITAMIN C , *CHARGE exchange - Abstract
Laser-induced graphene from paper (paper-LIG) was applied in non-enzymatic electrochemical sensing of uric acid (UA) in human urine. Paper-LIG was formed by CO 2 laser modification of paper into a 3D graphene arrangement. Kinetic analysis of paper-LIG electrodes returned effective heterogeneous electron transfer standard rate constants of 1.4 × 10−3 cm s−1 and 7.8 × 10−4 cm s−1 for [Ru(NH 3) 6 ]2+/3+ and [Fe(CN) 6 ]4−/3− redox probes, respectively. These electrodes were able to detect and quantify uric acid in PBS within the 10–300 μM range at pH between 5.6 and 7.4. At pH 7.4, a linear response (R2 = 0.999) from 10 to 250 μM was achieved, with a limit of detection of 3.97 μM and a sensitivity of 0.363 μA cm−2 μM−1. Paper-LIG electrodes denoted adequate selectivity in synthetic urine as well as in ascorbic acid (AA) and dopamine (DA)-containing electrolytes. Determination of urinary UA content in human samples returned a concentration of c.a. 1.8–1.9 mM, within the range for healthy individuals. Recoveries of samples spiked with 50 and 100 μM UA were 100.6% and 95.4%, respectively, with satisfactory reproducibility and stability. These cheap, lightweight, flexible, and eco-friendly paper-LIG biosensors for non-enzymatic quantification of UA in human urine pave the way to widespread application in the detection of other important biomarkers. [Display omitted] • "Green" graphene electrodes by CO 2 laser irradiation of paper ("paper-LIG"). • Uric acid (UA) quantification at clinically relevant concentrations and pH (5.6–7.4). • Limit of detection of 3.97 μM and a sensitivity of 0.363 μA cm−2 μM−1 (pH = 7.4). • Adequate selectivity in synthetic urine, as well as in ascorbic acid and dopamine-containing electrolytes. • Recoveries of 100.6% and 95.4% for human urine samples spiked with 50 and 100 μM UA. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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9. Cost-Effective Fiber Optic Solutions for Biosensing.
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Leitão, Cátia, Pereira, Sónia O., Marques, Carlos, Cennamo, Nunzio, Zeni, Luigi, Shaimerdenova, Madina, Ayupova, Takhmina, and Tosi, Daniele
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OPTICAL fiber detectors ,TUMOR markers ,OPTICAL transducers ,OPTICAL fibers ,ENVIRONMENTAL monitoring ,TRANSDUCERS ,FIBERS - Abstract
In the last years, optical fiber sensors have proven to be a reliable and versatile biosensing tool. Optical fiber biosensors (OFBs) are analytical devices that use optical fibers as transducers, with the advantages of being easily coated and biofunctionalized, allowing the monitorization of all functionalization and detection in real-time, as well as being small in size and geometrically flexible, thus allowing device miniaturization and portability for point-of-care (POC) testing. Knowing the potential of such biosensing tools, this paper reviews the reported OFBs which are, at the moment, the most cost-effective. Different fiber configurations are highlighted, namely, end-face reflected, unclad, D- and U-shaped, tips, ball resonators, tapered, light-diffusing, and specialty fibers. Packaging techniques to enhance OFBs' application in the medical field, namely for implementing in subcutaneous, percutaneous, and endoscopic operations as well as in wearable structures, are presented and discussed. Interrogation approaches of OFBs using smartphones' hardware are a great way to obtain cost-effective sensing approaches. In this review paper, different architectures of such interrogation methods and their respective applications are presented. Finally, the application of OFBs in monitoring three crucial fields of human life and wellbeing are reported: detection of cancer biomarkers, detection of cardiovascular biomarkers, and environmental monitoring. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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10. Relevance of the Spectral Analysis Method of Tilted Fiber Bragg Grating-Based Biosensors: A Case-Study for Heart Failure Monitoring.
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Vidal, Miguel, Soares, Maria Simone, Loyez, Médéric, Costa, Florinda M., Caucheteur, Christophe, Marques, Carlos, Pereira, Sónia O., and Leitão, Cátia
- Abstract
Optical fiber technology has rapidly progressed over the years, providing valuable benefits for biosensing purposes such as sensor miniaturization and the possibility for remote and real-time monitoring. In particular, tilted fiber Bragg gratings (TFBGs) are extremely sensitive to refractive index variations taking place on their surface. The present work comprises a case-study on the impact of different methods of analysis applied to decode spectral variations of bare and plasmonic TFBGs during the detection of N-terminal B-type natriuretic peptide (NT-proBNP), a heart failure biomarker, namely by following the most sensitive mode, peaks of the spectral envelopes, and the envelopes' crossing point and area. Tracking the lower envelope resulted in the lowest limits of detection (LOD) for bare and plasmonic TFBGs, namely, 0.75 ng/mL and 0.19 ng/mL, respectively. This work demonstrates the importance of the analysis method on the outcome results, which is crucial to attain the most reliable and sensitive method with lower LOD sensors. Furthermore, it makes the scientific community aware to take careful attention when comparing the performance of different biosensors in which different analysis methods were used. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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- View/download PDF
11. Optical Studies in Red/NIR Persistent Luminescent Cr-Doped Zinc Gallogermanate (ZGGO:Cr).
- Author
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Batista, Maria S., Rodrigues, Joana, Relvas, Maria S., Zanoni, Júlia, Girão, Ana V., Pimentel, Ana, Costa, Florinda M., Pereira, Sónia O., and Monteiro, Teresa
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PHOTOLUMINESCENCE measurement ,X-ray diffraction measurement ,LUMINESCENCE spectroscopy ,MOLE fraction ,ZINC ,LUMINESCENCE ,RAMAN spectroscopy - Abstract
Zn
1+x Ga2−2x Gex O4 (ZGGO:Cr)-persistent phosphor, with a molar fraction, x, of x = 0.1, doped with a 0.5% molar of chromium, was synthesised via solid-state reaction at 1350 °C for 36 h. X-ray diffraction measurements and Raman spectroscopy evidence a single crystalline phase corresponding to the cubic spinel structure. Room temperature (RT) photoluminescence (PL) and afterglow decay profiles were investigated using above and below bandgap excitation. In both cases, persistent PL was observed for almost 8 h, mainly originating from a Cr3+ defect, the so-called N2 optical centre. RT PL excitation and diffuse reflectance allow identification of the best pathways of Cr3+ red/NIR emission, as well as estimation of the ZGGO bandgap energy at 4.82 eV. An in-depth investigation of the observed luminescence at 15 K and temperature-dependent PL under site-selective excitation reveals the spectral complexity of the presence of several optically active Cr3+ centres in the ZGGO host that emit in almost the same spectral region. Furthermore, the temperature dependence of the R-lines' intensity indicates the existence of thermal populating processes between the different optical centres. Such observations well account for a wide distribution of defect trap levels available for carrier capture/release, as measured by the persistent luminescence decay, from which the carriers are released preferentially to the N2 Cr3+ -related optical centre. [ABSTRACT FROM AUTHOR]- Published
- 2022
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12. Label-Free Nanoscale ZnO Tetrapod-Based Transducers for Tetracycline Detection.
- Author
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Brás, Mariana, Zanoni, Julia, Falcão, Bruno P., Leitão, Joaquim P., Costa, Florinda M., Monteiro, Teresa, Pereira, Sónia O., and Rodrigues, Joana
- Abstract
Antibiotic pollution of freshwaters and even food products has become an important concern worldwide. Hence, it is of utmost importance to develop cost-effective and reliable devices that can provide information on the presence of such contaminants to the general population. In the present work, zinc oxide (ZnO) nanotetrapods (NTP) produced via a high yield laser processing approach were used as transducers in a luminescent-based immunosensor to detect tetracycline (TC). These tetrapodal structures present needle-shaped branches with a high aspect ratio, exhibiting lengths from hundreds of nanometers to a few micrometers and an average thickness of tens of nanometers, providing a high surface area for bioreceptor immobilization and analyte reaction, which is quite desirable in a transducer material. Besides, these ZnO NTP display intense photoluminescence (PL) at room temperature, making such a signal rather promising for transduction. Indeed, the intensity of the ZnO PL signal was seen to correlate with the TC concentration. The PL quenching with increasing analyte concentration is explained considering the rise in the bending of the electronic bands of the semiconductor near its surface due to increased charge density at this region, induced by the interaction between the bioreceptor (anti-TC antibodies) and the TC molecules. As a larger depletion width (and potential barrier) is promoted near the surface, the excitonic recombination probability is reduced and, consequently, the PL intensity in the ultraviolet spectral region, allowing us to use this relationship as a sensing mechanism. This information enabled us to define a calibration curve for TC quantification in the 0.001 to 1 μg L
–1 range, which is the range of interest of this antibiotic in freshwaters. A limit of detection (LOD) of ∼1.2 ng L–1 is reported, corresponding to one of the lowest LOD found in the literature for this antibiotic, indicating that the present ZnO NTP-based biosensors rival the current state-of-the-art ones. [ABSTRACT FROM AUTHOR]- Published
- 2022
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13. IR and UV Laser‐Induced Graphene: Application as Dopamine Electrochemical Sensors.
- Author
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Santos, Nuno F., Pereira, Sónia O., Moreira, Ana, Girão, Ana V., Carvalho, Alexandre F., Fernandes, António J. S., and Costa, Florinda M.
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ELECTROCHEMICAL sensors , *DOPAMINE , *CHARGE exchange , *GRAPHENE , *VITAMIN C - Abstract
Laser‐induced graphene (LIG) is inexpensive, fast, and easy to produce when compared to many other forms of graphene. Within the biosensing field, LIG electrodes are most often produced via infrared (IR) laser irradiation of polyimide sheets. Nevertheless, the usage of ultraviolet (UV) laser to produce LIG provides advantages in terms of sensor miniaturization because of its inherently higher scribing resolution. Yet, studies on the electrochemical performance of UV LIG, its relation with morphological and structural aspects as well as its comparison with IR LIG are still lacking. This work shows that both LIGs present swift electron transfer kinetics constituting excellent electrodes for electroanalysis. Extreme sensitivities of 93 and 58 µA µm−1 cm−2 at physiologically relevant dopamine (DA) concentrations are found for IR and UV LIG, respectively. Such sensitivities and good selectivity are achieved in the presence of physiologically relevant concentrations of ascorbic and uric acids, contrasting to the related literature employing IR LIG where such interferents are below the physiological range. Despite providing lower sensitivity, UV LIG is still an excellent material for DA biosensors, with the above‐mentioned advantages in terms of miniaturization. To our knowledge, these are among the highest sensitivities reported for voltammetric measurement of DA using carbon‐based materials. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
14. Physical Structure and Electrochemical Response of Diamond–Graphite Nanoplatelets: From CVD Synthesis to Label-Free Biosensors.
- Author
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Santos, Nuno F., Pereira, Sónia O., Fernandes, António J. S., Vasconcelos, Thiago L., Fung, Chung M., Archanjo, Bráulio S., Achete, Carlos A., Teixeira, Sofia R., Silva, Rui F., and Costa, Florinda M.
- Published
- 2019
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15. Polymer@gold Nanoparticles Prepared via RAFT Polymerization for Opto-Biodetection.
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Pereira, Sónia O., Barros-Timmons, Ana, and Trindade, Tito
- Subjects
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COLLOIDAL gold , *GOLD nanoparticles , *REVERSIBLE phase transitions , *CHAIN transfer (Chemistry) , *SURFACE plasmon resonance , *BIOSENSORS - Abstract
Colloidal gold nanoparticles (Au NPs) have been used in several biological applications, which include the exploitation of size- and shape-dependent Localized Surface Plasmon Resonance (LSPR) in biosensing devices. In order to obtain functional and stable Au NPs in a physiological medium, surface modification and functionalization are crucial steps in these endeavors. Reversible addition-fragmentation chain transfer (RAFT) polymerization meets this need offering the possibility of control over the composition and architecture of polymeric shells coating Au NPs. Furthermore, playing with a careful choice of monomers, RAFT polymerization allows the possibility to design a polymer shell with the desired functional groups aiming at Au based nanocomposites suitable for biorecognition and biotargeting. This review provides important aspects concerning the synthesis and optical properties of Au NPs as well as concepts of RAFT polymerization. Understanding these concepts is crucial to appreciate the chemical strategies available towards RAFT-polymer coated Au core-shell nanostructures, which are here reviewed. Finally, examples of applications in opto-biodetection devices are provided and the potential of responsive "smart" nanomaterials based on such structures can be applied to other biological applications. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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16. A Comparative Study of Chemical Routes for Coating Gold Nanoparticles via Controlled RAFT Emulsion Polymerization.
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Pereira, Sónia O., Barros‐Timmons, Ana, and Trindade, Tito
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GOLD nanoparticle synthesis , *EMULSION polymerization , *POLYMERIC nanocomposites , *AMPHIPHILES , *BLOCK copolymers , *METHYL acrylate , *POLYETHYLENE glycol , *SURFACE plasmon resonance - Abstract
The synthesis of core-shell Au nanoparticles protected by an amphiphilic block copolymer is investigated by distinct reversible addition fragmentation chain transfer (RAFT) emulsion polymerization routes. The controlled polymerization of polymer shells onto Au nanoparticles is attempted by using the macroRAFT (MR) agent based on 2-(dodecylthiocarbonothioylthio)-2-methylpropionic acid synthesized via RAFT polymerization of poly(ethylene glycol) methyl ether acrylate and exploring several approaches, which include (i) post-modification; (ii) in situ synthesis and (iii) 'grafting from' strategies. In the conditions investigated here all these strategies lead to Au polymer nanocomposites but morphological well-defined core-shell nanoparticles are only obtained by applying the 'grafting from' strategy. In particular, conditions that promote chain extension from the MR agent adsorbed onto the Au nanoparticles are found necessary to obtain nanostructures with such morphological characteristics and that still show the localized surface plasmon resonance typical of colloidal Au nanoparticles. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
17. ZnO Transducers for Photoluminescence-Based Biosensors: A Review.
- Author
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Rodrigues, Joana, Pereira, Sónia O., Zanoni, Julia, Rodrigues, Carolina, Brás, Mariana, Costa, Florinda M., and Monteiro, Teresa
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WARNING labels ,WIDE gap semiconductors ,OPTICAL transducers ,ZINC oxide ,BIOSENSORS ,TRANSDUCERS - Abstract
Zinc oxide (ZnO) is a wide bandgap semiconductor material that has been widely explored for countless applications, including in biosensing. Among its interesting properties, its remarkable photoluminescence (PL), which typically exhibits an intense signal at room temperature (RT), arises as an extremely appealing alternative transduction approach due to the high sensitivity of its surface properties, providing high sensitivity and selectivity to the sensors relying on luminescence output. Therefore, even though not widely explored, in recent years some studies have been devoted to the use of the PL features of ZnO as an optical transducer for detection and quantification of specific analytes. Hence, in the present paper, we revised the works that have been published in the last few years concerning the use of ZnO nanostructures as the transducer element in different types of PL-based biosensors, namely enzymatic and immunosensors, towards the detection of analytes relevant for health and environment, like antibiotics, glucose, bacteria, virus or even tumor biomarkers. A comprehensive discussion on the possible physical mechanisms that rule the optical sensing response is also provided, as well as a warning regarding the effect that the buffer solution may play on the sensing experiments, as it was seen that the use of phosphate-containing solutions significantly affects the stability of the ZnO nanostructures, which may conduct to misleading interpretations of the sensing results and unreliable conclusions. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
18. Immunosensing Based on Optical Fiber Technology: Recent Advances.
- Author
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Soares, Maria Simone, Vidal, Miguel, Santos, Nuno F., Costa, Florinda M., Marques, Carlos, Pereira, Sónia O., and Leitão, Cátia
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OPTICAL fibers ,OPTICAL fiber detectors ,SURFACE plasmon resonance ,OPTICAL gratings ,LIGHT transmission ,LIGHT metals - Abstract
The evolution of optical fiber technology has revolutionized a variety of fields, from optical transmission to environmental monitoring and biomedicine, given their unique properties and versatility. For biosensing purposes, the light guided in the fiber core is exposed to the surrounding media where the analytes of interest are detected by different techniques, according to the optical fiber configuration and biofunctionalization strategy employed. These configurations differ in manufacturing complexity, cost and overall performance. The biofunctionalization strategies can be carried out directly on bare fibers or on coated fibers. The former relies on interactions between the evanescent wave (EW) of the fiber and the analyte of interest, whereas the latter can comprise plasmonic methods such as surface plasmon resonance (SPR) and localized SPR (LSPR), both originating from the interaction between light and metal surface electrons. This review presents the basics of optical fiber immunosensors for a broad audience as well as the more recent research trends on the topic. Several optical fiber configurations used for biosensing applications are highlighted, namely uncladded, U-shape, D-shape, tapered, end-face reflected, fiber gratings and special optical fibers, alongside practical application examples. Furthermore, EW, SPR, LSPR and biofunctionalization strategies, as well as the most recent advances and applications of immunosensors, are also covered. Finally, the main challenges and an outlook over the future direction of the field is presented. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
19. Electrochemical Response of Glucose Oxidase Adsorbed on Laser-Induced Graphene.
- Author
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Pereira, Sónia O., Santos, Nuno F., Carvalho, Alexandre F., Fernandes, António J. S., and Costa, Florinda M.
- Subjects
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GLUCOSE oxidase , *FLAVIN adenine dinucleotide , *QUINONE , *CHARGE exchange , *GRAPHENE , *QUINONE derivatives - Abstract
Carbon-based electrodes have demonstrated great promise as electrochemical transducers in the development of biosensors. More recently, laser-induced graphene (LIG), a graphene derivative, appears as a great candidate due to its superior electron transfer characteristics, high surface area and simplicity in its synthesis. The continuous interest in the development of cost-effective, more stable and reliable biosensors for glucose detection make them the most studied and explored within the academic and industry community. In this work, the electrochemistry of glucose oxidase (GOx) adsorbed on LIG electrodes is studied in detail. In addition to the well-known electroactivity of free flavin adenine dinucleotide (FAD), the cofactor of GOx, at the expected half-wave potential of −0.490 V vs. Ag/AgCl (1 M KCl), a new well-defined redox pair at 0.155 V is observed and shown to be related to LIG/GOx interaction. A systematic study was undertaken in order to understand the origin of this activity, including scan rate and pH dependence, along with glucose detection tests. Two protons and two electrons are involved in this reaction, which is shown to be sensitive to the concentration of glucose, restraining its origin to the electron transfer from FAD in the active site of GOx to the electrode via direct or mediated by quinone derivatives acting as mediators. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
20. Dual Transduction of H 2 O 2 Detection Using ZnO/Laser-Induced Graphene Composites.
- Author
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Zanoni, Julia, Moura, Jorge P., Santos, Nuno F., Carvalho, Alexandre F., Fernandes, António J. S., Monteiro, Teresa, Costa, Florinda M., Pereira, Sónia O., Rodrigues, Joana, Rovisco, Ana, and Comini, Elisabetta
- Subjects
GENETIC transduction ,GRAPHENE ,CHARGE exchange ,IMPEDANCE spectroscopy ,CYCLIC voltammetry ,ZINC oxide - Abstract
Zinc oxide (ZnO)/laser-induced graphene (LIG) composites were prepared by mixing ZnO, grown by laser-assisted flow deposition, with LIG produced by laser irradiation of a polyimide, both in ambient conditions. Different ZnO:LIG ratios were used to infer the effect of this combination on the overall composite behavior. The optical properties, assessed by photoluminescence (PL), showed an intensity increase of the excitonic-related recombination with increasing LIG amounts, along with a reduction in the visible emission band. Charge-transfer processes between the two materials are proposed to justify these variations. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy evidenced increased electron transfer kinetics and an electrochemically active area with the amount of LIG incorporated in the composites. As the composites were designed to be used as transducer platforms in biosensing devices, their ability to detect and quantify hydrogen peroxide (H
2 O2 ) was assessed by both PL and CV analysis. The results demonstrated that both methods can be employed for sensing, displaying slightly distinct operation ranges that allow extending the detection range by combining both transduction approaches. Moreover, limits of detection as low as 0.11 mM were calculated in a tested concentration range from 0.8 to 32.7 mM, in line with the values required for their potential application in biosensors. [ABSTRACT FROM AUTHOR]- Published
- 2021
- Full Text
- View/download PDF
21. Biofunctional Polymer Coated Au Nanoparticles Prepared via RAFT-Assisted Encapsulating Emulsion Polymerization and Click Chemistry.
- Author
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Pereira, Sónia O., Trindade, Tito, and Barros-Timmons, Ana
- Subjects
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CLICK chemistry , *EMULSION polymerization , *COLLOIDAL gold , *POLYMERS , *GOLD nanoparticles , *THERMORESPONSIVE polymers , *FOOD emulsions - Abstract
The use of reversible addition-fragmentation chain transfer (RAFT)-assisted encapsulating emulsion polymerization (REEP) has been explored to prepare diverse types of colloidal stable core–shell nanostructures. A major field of application of such nanoparticles is in emergent nanomedicines, which require effective biofunctionalization strategies, in which their response to bioanalytes needs to be firstly assessed. Herein, functional core–shell nanostructures were prepared via REEP and click chemistry. Thus, following the REEP strategy, colloidal gold nanoparticles (Au NPs, d = 15 nm) were coated with a poly(ethylene glycol) methyl ether acrylate (PEGA) macroRAFT agent containing an azide (N3) group to afford N3–macroRAFT@Au NPs. Then, chain extension was carried out from the NPs surface via REEP, at 44 °C under monomer-starved conditions, to yield N3–copolymer@Au NPs–core–shell type structures. Biotin was anchored to N3–copolymer@Au NPs via click chemistry using an alkynated biotin to yield biofunctionalized Au nanostructures. The response of the ensuing biotin–copolymer@Au NPs to avidin was followed by visible spectroscopy, and the copolymer–biotin–avidin interaction was further studied using the Langmuir–Blodgett technique. This research demonstrates that REEP is a promising strategy to prepare robust functional core–shell plasmonic nanostructures for bioapplications. Although the presence of azide moieties requires the use of low polymerization temperature, the overall strategy allows the preparation of tailor-made plasmonic nanostructures for applications of biosensors based on responsive polymer shells, such as pH, temperature, and photoluminescence quenching. Moreover, the interaction of biotin with avidin proved to be time dependent. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
22. Laser-induced graphene from commercial polyimide coated optical fibers for sensor development.
- Author
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Martins, Luís, Kulyk, Bohdan, Theodosiou, Antreas, Ioannou, Andreas, Moreirinha, Catarina, Kalli, Kyriacos, Santos, Nuno, Costa, Florinda, Pereira, Sónia O., and Marques, Carlos
- Subjects
- *
OPTICAL coatings , *BRAGG gratings , *OPTICAL fiber detectors , *GRAPHENE , *OPTICAL fibers , *OPTICAL transducers , *FEMTOSECOND lasers - Abstract
Direct laser scribing (DLS) technology is applied to commercial polyimide (PI) coated optical fibers producing laser-induced graphene (LIG) coated optical fibers. The PI-coated optical fiber is first inscribed with off-center Bragg gratings via point-by-point method using a femtosecond laser system. Then, the off-center Bragg grating is irradiated with a CO 2 laser and the PI coating is photothermally converted into LIG without affecting the grating. Scanning electron microscopy (SEM) and Raman spectroscopy prove the presence of the laser-induced graphene layer on the optical fiber, and Fourier transform infrared (FTIR) spectroscopy confirms that all the PI coating was converted into LIG. The resulting LIG-coated off-center Bragg grating (FBG) sensors respond to variations of the surrounding refractive index (RI) with a sensitivity of 38.08 ± 1.57 nm per RI units (RIU). In addition, after removal of the PI coating, obtaining a bare-FBG, the sensitivity decreases to 25.63 ± 0.66 nm R I U − 1 . Therefore, this strategy, here reported for the first time, paves the way to produce promising novel optical transducers aiming to develop bio- and chem-sensing devices. • Direct laser scribing technology applied to commercial polyimide optical fibers. • Laser-induced graphene produced directly on optical fiber for sensing. • The resulting LIG-coated off-center FBG sensors respond to variations of the surrounding RI. • High sensitivity compared with after removal of the PI coating. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
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