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A simple single step ion exchange chromatography method has been developed for purification of SA from plant cell culture extracts. The optimum washing and elution solvents are 25 mM sodium phosphate (pH 7.0-7.5) and 0.3 M HCl in 60% acetonitrile, respectively. Using the developed method the SA recoveries were found 69-80% calculated from the whole purification process. In particular, in the second 10 mL elution, 70 µg SA in 1 g resin yielded 65% SA recovery, which provided a well-detectable amount of SA with a relatively low level of impurities as analyzed by 400 MHz 1H-NMR. [ABSTRACT FROM AUTHOR]

Anthranilate synthase (EC 4.1.3.27) has been purified from cell cultures of Catharanthus roseus by poly(ethylene glycol) precipitation/fractionation and subsequent separation by anion exchange on Q-Sepharose, Orange A dye chromatography, Mono Q anion-exchange chromatography and Superose 6 gel filtration. By analogy to anthranilate synthases from other sources it does look like the enzyme is a tetramer composed of two large and two small subunits, with molecular mass 67 and 25.5 ± 0.5 kDa, respectively. The molecular mass determined by gel filtration was 143 ± 5 kDa. The enzyme had a pI of 5.1 determined by chromatofocusing. The pH optimum was between pH 7.5 H and pH 8,3 but the type of buffer used cted the results, The enzyme could utilize N4+ as ammonium donor instead of glutamine. The enzyme showed normal Michaelis-Menten kinetics with respect to the substrates L-glutamine and chorismate, and the cofactor Mg2+, Km values for L-glutamine was determined to be 0.37 ± 0.05 mM, for chorismate 67 ± 3 μM, and for MgCl2 0.26 ± 0.03 mM respectively. Anthranilate synthase was inhibited by L-tryptophan, tryptamine and D-tryptophan (with L-tryptophan being the best inhibitor). The enzyme was allosterically regulated showing positive cooperativity of chorismate binding at higher concentrations of tryptophan. For a tryptophan concentration of 20 μM the Hill coefficient was determined to be 2. The tryptophan binding sites showed positive cooperativity for higher concentrations of chorismate. The purified enzyme did not contain anthranilate-5-phosphoribosylpyrophosphate phosphoribosyltransferase activity and is thus not of the same type as the well characterized Salmonella typhimurium anthranilate synthase/phosphoribosyl pyrophosphate transferase bifunctional type. [ABSTRACT FROM AUTHOR]