11 results on '"Miao, Sh."'
Search Results
2. Increased NOX2 expression in astrocytes leads to eNOS uncoupling through dihydrofolate reductase in endothelial cells after subarachnoid hemorrhage.
- Author
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Miao SH, Gao SQ, Li HX, Zhuang YS, Wang X, Li T, Gao CC, Han YL, Qiu JY, and Zhou ML
- Abstract
Introduction: Endothelial nitric oxide synthase (eNOS) uncoupling plays a significant role in acute vasoconstriction during early brain injury (EBI) after subarachnoid hemorrhage (SAH). Astrocytes in the neurovascular unit extend their foot processes around endothelia. In our study, we tested the hypothesis that increased nicotinamide adenine dinucleotide phosphate oxidase 2 (NOX2) expression in astrocytes after SAH leads to eNOS uncoupling., Methods: We utilized laser speckle contrast imaging for monitoring cortical blood flow changes in mice, nitric oxide (NO) kits to measure the level of NO, and a co-culture system to study the effect of astrocytes on endothelial cells. Moreover, the protein levels were assessed by Western blot and immunofluorescence staining. We used CCK-8 to measure the viability of astrocytes and endothelial cells, and we used the H
2 O2 kit to measure the H2 O2 released from astrocytes. We used GSK2795039 as an inhibitor of NOX2, whereas lentivirus and adeno-associated virus were used for dihydrofolate reductase (DHFR) knockdown in vivo and in vitro ., Results: The expression of NOX2 and the release of H2 O2 in astrocytes are increased, which was accompanied by a decrease in endothelial DHFR 12 h after SAH. Moreover, the eNOS monomer/dimer ratio increased, leading to a decrease in NO and acute cerebral ischemia. All of the above were significantly alleviated after the administration of GSK2795039. However, after knocking down DHFR both in vivo and in vitro , the protective effect of GSK2795039 was greatly reversed., Discussion: The increased level of NOX2 in astrocytes contributes to decreased DHFR in endothelial cells, thus aggravating eNOS uncoupling, which is an essential mechanism underlying acute vasoconstriction after SAH., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Miao, Gao, Li, Zhuang, Wang, Li, Gao, Han, Qiu and Zhou.)- Published
- 2023
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3. Dobutamine promotes the clearance of erythrocytes from the brain to cervical lymph nodes after subarachnoid hemorrhage in mice.
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Wang X, Deng HJ, Gao SQ, Li T, Gao CC, Han YL, Zhuang YS, Qiu JY, Miao SH, and Zhou ML
- Abstract
Background: Erythrocytes and their breakdown products in the subarachnoid space (SAS) are the main contributors to the pathogenesis of subarachnoid hemorrhage (SAH). Dobutamine is a potent β
1 -adrenoreceptor agonist that can increase cardiac output, thus improving blood perfusion and arterial pulsation in the brain. In this study, we investigated whether the administration of dobutamine promoted the clearance of red blood cells (RBCs) and their degraded products via meningeal lymphatic vessels (mLVs), thus alleviating neurological deficits in the early stage post-SAH. Materials and methods: Experimental SAH was induced by injecting autologous arterial blood into the prechiasmatic cistern in male C57BL/6 mice. Evans blue was injected into the cisterna magna, and dobutamine was administered by inserting a femoral venous catheter. RBCs in the deep cervical lymphatic nodes (dCLNs) were evaluated by hematoxylin-eosin staining, and the hemoglobin content in dCLNs was detected by Drabkin's reagent. The accumulation of RBCs in the dura mater was examined by immunofluorescence staining, neuronal death was evaluated by Nissl staining, and apoptotic cell death was evaluated by TUNEL staining. The Morris water maze test was used to examine the cognitive function of mice after SAH. Results: RBCs appeared in dCLNs as early as 3 h post-SAH, and the hemoglobin in dCLNs peaked at 12 h after SAH. Dobutamine significantly promoted cerebrospinal fluid (CSF) drainage from the SAS to dCLNs and obviously reduced the RBC residue in mLVs, leading to a decrease in neuronal death and an improvement in cognitive function after SAH. Conclusion: Dobutamine administration significantly promoted RBC drainage from cerebrospinal fluid in the SAS via mLVs into dCLNs, ultimately relieving neuronal death and improving cognitive function., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Wang, Deng, Gao, Li, Gao, Han, Zhuang, Qiu, Miao and Zhou.)- Published
- 2023
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4. Multidifferentiation potential of dental-derived stem cells.
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Yin JY, Luo XH, Feng WQ, Miao SH, Ning TT, Lei Q, Jiang T, and Ma DD
- Abstract
Tooth-related diseases and tooth loss are widespread and are a major public health issue. The loss of teeth can affect chewing, speech, appearance and even psychology. Therefore, the science of tooth regeneration has emerged, and attention has focused on tooth regeneration based on the principles of tooth development and stem cells combined with tissue engineering technology. As undifferentiated stem cells in normal tooth tissues, dental mesenchymal stem cells (DMSCs), which are a desirable source of autologous stem cells, play a significant role in tooth regeneration. Researchers hope to reconstruct the complete tooth tissues with normal functions and vascularization by utilizing the odontogenic differentiation potential of DMSCs. Moreover, DMSCs also have the ability to differentiate towards cells of other tissue types due to their multipotency. This review focuses on the multipotential capacity of DMSCs to differentiate into various tissues, such as bone, cartilage, tendon, vessels, neural tissues, muscle-like tissues, hepatic-like tissues, eye tissues and glands and the influence of various regulatory factors, such as non-coding RNAs, signaling pathways, inflammation, aging and exosomes, on the odontogenic/osteogenic differentiation of DMSCs in tooth regeneration. The application of DMSCs in regenerative medicine and tissue engineering will be improved if the differentiation characteristics of DMSCs can be fully utilized, and the factors that regulate their differentiation can be well controlled., Competing Interests: Conflict-of-interest statement: Authors declare that they have no conflicts of interest for this article., (©The Author(s) 2021. Published by Baishideng Publishing Group Inc. All rights reserved.)
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- 2021
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5. [Diffusion tensor imaging and visual evoked potentials in pediatric patients with sellar region lesions].
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Luo B, Miao SH, He L, Zhao YP, Xu CW, Zhu J, Zhang QH, Liu W, Ma Y, and Zhang YQ
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- Anisotropy, Child, Diffusion Magnetic Resonance Imaging, Evoked Potentials, Visual, Humans, Diffusion Tensor Imaging, Eye Diseases
- Abstract
Objiective: To evaluate the prognosis of visual function and the impact of surgery in pediatric patients with sellar mass lesions, as evidenced by diffusion tensor imaging (DTI) and visual evoked potentials. Methods: Twenty patients with sellar mass lesions were included in the study. DTI and visual evoked potentials were obtained before and after surgery. Fractional anisotropy (FA) and apparent diffusion coefficient (ADC) values were calculated for both optic nerves. DTI parameters and visual evoked potential amplitudes were compared for all patients to assess the correlation between DTI parameters and visual function. Results: The 20 patients were divided into two groups according the relationship between the lesions and the optic chiasm. The FA values increased significantly after operation, while the ADC values decreased ( P <0.05). And the average amplitude of visual evoked potentials after operation was significantly higher than before operation ( P< 0.05). Conclusions: DTI assessments of the affected sides, with the resulting FA and ADC values, may help to estimate the visual improvement produced by surgical therapy in the early postoperative period. Surgical removal can improve visual function dramatically.
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- 2019
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6. Effect of telehealth interventions on major cardiovascular outcomes: a meta-analysis of randomized controlled trials.
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Gu X, Zhu Y, Zhang Y, Sun L, Bao ZY, Shen JH, Chen FK, Li HX, Miao SH, Wang JW, and Shi QQ
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Background: Telehealth interventions (THI) were associated with lower levels of cardiovascular risk factors in adults, whereas the effect of THI on cardiovascular disease (CVD) still remains controversial. A meta-analysis was conducted to summarize the evidence from randomized controlled trials (RCT) which investigated potential impact of THI on the incidence of CVD in patients with or without prior CVD., Methods: PubMed, EmBase, and the Cochrane Library were searched to identify RCTs to fit our analysis through December 2016. Relative risk (RR) with its 95% confidence interval (CI) was used to measure the effect of THI using a random-effect model. Sensitivity analysis, subgroup analysis, heterogeneity tests, and tests for publication bias were also conducted., Results: Eight RCTs were included and with a total of 1635 individuals. The summarized results indicated that participants who received THI showed a significant reduction of the CVD incidence as compared with usual care (RR: 0.59; 95% CI: 0.47-0.74; P < 0.001). Furthermore, the effect of THI was greater in patients with history of CVD (RR: 0.55; 95% CI: 0.44-0.70; P < 0.001) than in patients without history of CVD (RR: 0.99; 95% CI: 0.51-1.94; P = 0.977). Sensitivity analysis suggested that the intervention effect persisted and the conclusion was not changed. Subgroup analysis indicated mean age, study quality might play an important role on the risk of CVD., Conclusions: The findings of this study indicated THI could reduce the recurrence of CVD. Further large-scale trials are needed to verify the effect of THI on CVD in healthy individuals.
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- 2017
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7. Trigeminal somatosensory-evoked potential: A neurophysiological tool to monitor the extent of lesion of ganglion radiofrequency thermocoagulation in idiopathic trigeminal neuralgia: A case-control study.
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Zhao YX, Miao SH, Tang YZ, He LL, Yang LQ, Ma Y, and Ni JX
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- Aged, Case-Control Studies, Female, Humans, Male, Middle Aged, Outcome Assessment, Health Care methods, Ablation Techniques, Evoked Potentials, Somatosensory, Trigeminal Nerve physiology, Trigeminal Neuralgia therapy
- Abstract
To reflect the extent of thermolesion of ganglion by testing the change of trigeminal somatosensory-evoked potential (TSEP) before and after ganglion radiofrequency thermocoagulation surgery (GRT), and evaluate long-term clinic effect by follow-up visiting of 1 year.Patients with idiopathic trigeminal neuralgia (TN) in the second division were enrolled between October 2014 and October 2015. They were treated with computed tomography-guided GRT and a follow-up visiting of 1 year. Bilateral TSEP measurements were performed 1 day before and 2 days after the GRT surgery. The latency and peak-to-peak amplitude of W2 and W3 were recorded.Immediate postprocedure pain relief (grades I-III) was 100% and 92.5% 1 year later. Facial numbness rate of grades III and IV was 70%, 40%, and 12.5%, respectively, at immediate, 2 days, and 1 year after GRT. No sever complications happened. The latency of W2 and W3 of patients who had no pain no numbness after 1 year of GRT was 1.74 ± 0.24 and 3.84 ± 0.66 ms, respectively, of TN side, and 1.71 ± 0.39 and 3.63 ± 0.85 ms of the healthy side before GRT. The amplitude of W2 and W3 was 1.13 ± 0.50 and 1.99 ± 1.09 uv, respectively, of TN side and 1.24 ± 0.40 and 1.89 ± 0.81 uv of the healthy side before GRT. There was no statistical difference of the latency and amplitude between 2 sides of W2 and W3 before surgery (P > 0.05). The latency of W2 and W3 delayed and the amplitude reduced especially in TN side after surgery comparing before (P < 0.001). And, comparisons of the latency and amplitude of W2 and W3 between TN side and the healthy side after surgery showed the latency of W2 and W3 delayed (W2: P = 0.02; W3: P = 0.01) and the amplitude of W2 reduced (P = 0.003), but the amplitude of W3 had no statistical difference (P = 0.22). The mean delayed latency and 95% confident interval of W2 and W3 were 0.22 ± 0.35 (0.1-0.34) ms and 0.35 ± 0.64 (0.14-0.57) ms, respectively. The mean decreased amplitude and 95% confident interval of W2 and W3 were 22 ± 24 (14-30)% and 23 ± 32 (12-34)%, respectively.GRT can make the latency delay and the amplitude decrease of TSEP. And the latency and amplitude of W2 and W3 can be considered reliable and safe reference for monitoring the extent of thermolesion., Competing Interests: The authors have no conflicts of interest to disclose.
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- 2017
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8. Astrocytic JWA expression is essential to dopaminergic neuron survival in the pathogenesis of Parkinson's disease.
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Miao SH, Sun HB, Ye Y, Yang JJ, Shi YW, Lu M, Hu G, and Zhou JW
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- 3,4-Dihydroxyphenylacetic Acid metabolism, Animals, Carrier Proteins genetics, Cell Line, Cell Survival drug effects, Cell Survival genetics, Disease Models, Animal, Glial Fibrillary Acidic Protein genetics, Glial Fibrillary Acidic Protein metabolism, Glutathione metabolism, Heat-Shock Proteins, Homovanillic Acid metabolism, I-kappa B Kinase metabolism, Membrane Transport Proteins, Mice, Mice, Transgenic, Reactive Oxygen Species metabolism, Signal Transduction drug effects, Signal Transduction physiology, Substantia Nigra pathology, Transfection, Astrocytes metabolism, Carrier Proteins metabolism, Dopamine metabolism, MPTP Poisoning pathology, Neurons pathology
- Abstract
Aims: To investigate the role of astrocytic JWA expression in dopaminergic (DA) neuron degeneration and in the pathogenesis of Parkinson's disease (PD)., Methods: Conditional astrocytic JWA null (JWA∆2/∆2/GFAP-Cre) mice and U251 glioma cells were used to evaluate the effects of JWA gene on DA neuron degeneration. The oxidative stress-driven molecular events were determined in both in vivo and in vitro models., Results: Conditional astrocytic JWA knockout resulted in significant activation of astrocytes measured by increase in glial fibrillary acidic protein-positive cells (1.34×10(3)±74.5 vs. 8.44×10(3)±1.35×10(3), P<0.01) in mouse substantia nigra, accompanied by loss of DA neurons (1.03×10(4)±238 vs. 6.17×10(3)±392, P<0.001). Deficiency of JWA significantly aggravated reactive oxygen species (ROS) accumulation in substantia nigra compared with the wild-type mice. Increasing JWA expression in U251 glioma cells inhibited ROS with a concomitant increase in intracellular glutathione. Furthermore, suppression of IKKβ-nuclear factor (NF)-κB signaling pathway was shown to regulate JWA in a PD model., Conclusions: The JWA gene exerts neuroprotective roles against DA neuronal degeneration via modulating intracellular redox status and NF-κB signaling pathway and is a potential treatment target for PD., (© 2014 John Wiley & Sons Ltd.)
- Published
- 2014
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9. Prognostic value of caveolin-1 expression in gastric cancer: a meta-analysis.
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Ye Y, Miao SH, Lu RZ, and Zhou JW
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- Case-Control Studies, Humans, Prognosis, Risk Factors, Caveolin 1 genetics, Genetic Predisposition to Disease, Polymorphism, Genetic genetics, Stomach Neoplasms genetics
- Abstract
The relationship between caveolin-1 (Cav-1) and clinicopathological characteristics of gastric cancer is controversial, although Cav-1 plays an important role in tumor metastasis. To evaluate the clinicopathological and prognostic value of expression in patients with gastric cancer, a meta-analysis was performed to investigate the impact on clinicopathological parameters and prognosis in gastric cancer cases. Studies assessing these parameters for Cav-1 in gastric cancer were identified up to June 2014. Finally, a total of six studies met the inclusion criteria. Our combined results showed that Cav-1 expression was significantly associated with the Lauren classification (pooled OR=0.603, 95% CI: 0.381-0.953, P=0.030). Furthermore, we found that Cav-1 expression predicted a better overall survival in gastric cancer patients (pooled OR=0.590, 95% CI: 0.360-0.970, P=0.038, fixed-effect). In conclusion, the overall data of the present meta analysis showed that Cav-1 expression was not correlated with clinicopathological features except for the Lauren classification. Simultaneously, Cav-1 overexpression predicted a better overall survival in gastric cancer. Cav-1 expression in tumors is a candidate positive prognostic biomarker for gastric cancer patients.
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- 2014
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10. Neuronal firing in the ventrolateral thalamus of patients with Parkinson's disease differs from that with essential tremor.
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Chen H, Zhuang P, Miao SH, Yuan G, Zhang YQ, Li JY, and Li YJ
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- Female, Humans, Male, Middle Aged, Retrospective Studies, Essential Tremor physiopathology, Neurons physiology, Parkinson Disease physiopathology, Ventral Thalamic Nuclei physiopathology
- Abstract
Background: Although thalamotomy could dramatically improve both parkinsonian resting tremor and essential tremor (ET), the mechanisms are obviously different. This study aimed to investigate the neuronal activities in the ventrolateral thalamus of Parkinson's disease (PD) and ET., Methods: Thirty-six patients (PD: 20, ET: 16) were studied. Microelectrode recordings in the ventral oral posterior (Vop) and the ventral intermediate nucleus (Vim) of thalamus was performed on these patients who underwent thalamotomy. Electromyography (EMG) was recorded simultaneously on the contralateral limbs to surgery. Single unit analysis and the interspike intervals (ISIs) were measured for each neuronal type. ISI histogram and auto-correlograms were constructed to estimate the pattern of neuronal firing. Mann-Whitney test and Kruskal-Wallis (K-W) test were used to compare the mean spontaneous firing rate (MSFR) of neurons of PD and ET patients., Results: Three hundred and twenty-three neurons were obtained from 20 PD trajectories, including 151 (46.7%) tremor related neuronal activity, 74 neurons (22.9%) with tonic firing, and 98 (30.4%) neurons with irregular discharge. One hundred and eighty-seven neurons were identified from 16 ET trajectories including 46 (24.6%) tremor-related neuronal activity, 77 (41.2%) neurons with tonic firing, and 64 neurons (34.2%) with irregular discharge. The analysis of MSFR of neurons with tonic firing was 26.7 (3.4 - 68.3) Hz (n = 74) and that of neurons with irregular discharge (n = 98) was 13.9 (3.0 - 58.1) Hz in PD; whereas MSFR of neurons with tonic firing (n = 77) was 48.8 (19.0 - 135.5) Hz and that of neurons with irregular discharge (n = 64) was 26.3 (8.7 - 84.7) Hz in ET. There were significant differences in the MSFR of two types of neuron for PD and ET (K-W test, both P < 0.05). Significant differences in the MSFR of neuron were also obtained from Vop and Vim of PD and ET (16.3 Hz vs. 34.8 Hz, 28.0 Hz vs. 49.9 Hz) (K-W test, both P < 0.05), respectively., Conclusion: In consistent with recent findings, the decreased MSFR of neurons observed in the Vop is likely to be involved in PD whereas the increased MSFR of neurons seen in the Vim may be a cause of ET.
- Published
- 2010
11. [Neuronal activity of ventrolateral thalamus in patients with essential tremor].
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Miao SH, Zhuang P, Li JY, and Li YJ
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- Adolescent, Adult, Aged, Female, Humans, Male, Microelectrodes, Middle Aged, Parkinson Disease physiopathology, Ventral Thalamic Nuclei cytology, Young Adult, Essential Tremor physiopathology, Ventral Thalamic Nuclei physiopathology
- Abstract
Objective: To investigate the neuronal activity in the ventrolateral thalamus in relation to essential tremor (ET)., Methods: Microelectrode recording in the ventral oral posterior (Vop) and ventral intermediate nucleus (Vim) of thalamus was performed on 10 patients with ET and 10 patients with Parkinson's disease (PD) who underwent thalamotomy for tremor during operation. Electromyography (EMG) was carried out on the contralateral limbs simultaneously. Single unit analysis was performed to measure the interspike interval (ISI) and histogram was constructed to evaluate the pattern of neuronal activity. Student t-test was employed to compare the mean spontaneous firing rate (MSFR) and ISI of neuronal firing in Vop/Vim between the ET patients and PD patients. and correlation test., Results: Two hundred and sixty-six neurons were identified from 20 trajectories, 38.0% being neurons with tremor-related neuronal activity Tremor cells, 31.9% of neurons related to tonic firing, and 30.1% of neurons related to irregular discharge. 131 of these thalamic neurons, were obtained from 10 ET patients. 38 of these 131 neurons (29.0%) were related to tremor-related neuronal activity, 54 (41.2%) neurons were related to tonic firing with a mean spontaneous firing rate (MSFR) of (55+/-21) Hz, and 39 neurons (29.8%) were related to irregular discharge with a MSFR of (32+/-17) Hz. In the meantime, 135 neurons were obtained from 10 PD patients. Of these 135 neurons 63 (46.7%) were related to tremor, 31 neurons (23.0%) related to tonic firing with a MSFR of (39+/-15) Hz, and 41 (30.3%) were related to irregular discharge with a MSFR of (21+/-8) Hz. The MSFR levels of pooled neurons in Vop/Vim with tonic firing and irregular discharge of the ET patients were significantly higher than those of the PD patents (t=2.74 and 2.99, both P<0.05). And the MSFR levels in Vop and Vim of the ET patients were significantly higher than those of the PD patients (both P<0.05)., Conclusion: Vim receives the projection from cerebellum. The increase of neuronal firing frequency observed in Vim suggests that the pathophysiology of ET may be correlated with excessive activity of cerebellum.
- Published
- 2009
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