1. A Kelch Motif-Containing Serine/Threonine Protein Phosphatase Determines the Large Grain QTL Trait in Rice.
- Author
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Hu, Zejun, He, Haohua, Zhang, Shiyong, Sun, Fan, Xin, Xiaoyun, Wang, Wenxiang, Qian, Xi, Yang, Jingshui, and Luo, Xiaojin
- Subjects
SERINE/THREONINE kinases ,PHOSPHOPROTEIN phosphatases ,RICE genetics ,LOCUS in plant genetics ,RICE varieties ,PLANT chromosomes ,PLANT gene mapping - Abstract
A thorough understanding of the genetic basis of rice grain traits is critical for the improvement of rice ( Oryza sativa L.) varieties. In this study, we generated an F
2 population by crossing the large-grain japonica cultivar CW23 with Peiai 64 (PA64), an elite indica small-grain cultivar. Using QTL analysis, 17 QTLs for five grain traits were detected on four different chromosomes. Eight of the QTLs were newly-identified in this study. In particular, qGL3-1, a newly-identified grain length QTL with the highest LOD value and largest phenotypic variation, was fine-mapped to the 17 kb region of chromosome 3. A serine/threonine protein phosphatase gene encoding a repeat domain containing two Kelch motifs was identified as the unique candidate gene corresponding to this QTL. A comparison of PA64 and CW23 sequences revealed a single nucleotide substitution (C→A) at position 1092 in exon 10, resulting in replacement of Asp (D) in PA64 with Glu (E) in CW23 for the 364th amino acid. This variation is located at the D position of the conserved sequence motif AVLDT of the Kelch repeat. Genetic analysis of a near-isogenic line (NIL) for qGL3-1 revealed that the allele qGL3-1 from CW23 has an additive or partly dominant effect, and is suitable for use in molecular marker-assisted selection. [ABSTRACT FROM AUTHOR]- Published
- 2012
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