1. Interspecific cooperation: enhanced growth, attachment and strain-specific distribution in biofilms through Azospirillum brasilense-Pseudomonas protegens co-cultivation
- Author
-
Luciana A. Pagnussat, Christoph Keel, Claudio Valverde, Guillermo Andrés Maroniche, Florencia Salcedo, and Cecilia M. Creus
- Subjects
0301 basic medicine ,BIOFILM STRUCTURE ,030106 microbiology ,Azospirillum brasilense ,PLANT-GROWTH-PROMOTING-BACTERIA ,Microbiology ,Plant Roots ,Ciencias Biológicas ,03 medical and health sciences ,Pseudomonas protegens ,Biología Celular, Microbiología ,Pseudomonas ,Botany ,Genetics ,COLONY PHENOTYPE ,Molecular Biology ,Microbial inoculant ,Soil Microbiology ,Rhizosphere ,biology ,Strain (chemistry) ,Biofilm ,Plants ,biology.organism_classification ,Coculture Techniques ,030104 developmental biology ,Biofilms ,CIENCIAS NATURALES Y EXACTAS ,Bacteria - Abstract
Plant-growth-promoting bacteria belonging to Azospirillum and Pseudomonas genera are major inhabitants of the rhizosphere. Both are increasingly commercialized as crops inoculants. Interspecific interaction in the rhizosphere is critical for inoculants aptness. The objective of this work was to evaluate Azospirillum and Pseudomonas interaction in mixed biofilms by co-cultivation of the model strains Azospirillum brasilense Sp245 and Pseudomonas protegens CHA0. The results revealed enhanced growth of both strains when co-cultured in static conditions. Moreover, Sp245 biofilm formed in plastic surfaces was increased 2-fold in the presence of CHA0. Confocal microscopy revealed highly structured mixed biofilms showing Sp245 mainly on the bottom and CHA0 towards the biofilm surface. In addition, A. brasilense biofilm was thicker and denser when co-cultured with P. protegens. In a colony-colony interaction assay, Sp245 changed nearby CHA0 producing small colony phenotype, which accounts for a diffusible metabolite mediator; though CHA0 spent medium did not affect Sp245 colony phenotype. Altogether, these results point to a cooperative interaction between A. brasilense Sp245 and P. protegens CHA0 in which both strains increase their static growth and produce structured mixed biofilms with a strain-specific distribution. Fil: Pagnussat, Luciana Anabella. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Salcedo, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; Argentina Fil: Maroniche, Guillermo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; Argentina Fil: Keel, Christoph. Universite de Lausanne; Suiza Fil: Valverde, Claudio Fabián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; Argentina Fil: Creus, Cecilia Mónica. Universidad Nacional de Mar del Plata; Argentina
- Published
- 2016