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Sport facilities represent extreme indoor environments due to intense cleaning and disinfection. The aim of this study was to describe the composition of the cultivated microbiota in dust samples collected in sport facilities during the COVID-19 pandemic. A dust sample is defined as the airborne dust sedimented on 0.02 m2 within 28 d. The results show that the microbial viable counts in samples of airborne dust (n = 9) collected from seven Finnish sport facilities during the pandemic contained a high proportion of pathogenic filamentous fungi and a low proportion of bacteria. The microbial viable counts were between 14 CFU and 189 CFU per dust sample. In seven samples from sport facilities, 20–85% of the microbial viable counts were fungi. Out of 123 fungal colonies, 47 colonies belonged to the potentially pathogenic sections of Aspergillus (Sections Fumigati, Nigri, and Flavi). Representatives of each section were identified as Aspergillus fumigatus, A. flavus, A. niger and A. tubingensis. Six colonies belonged to the genus Paecilomyces. In six samples of dust, a high proportion (50–100%) of the total fungal viable counts consisted of these potentially pathogenic fungi. A total of 70 isolates were considered less likely to be pathogenic, and were identified as Aspergillus section Nidulantes, Chaetomium cochliodes and Penicillium sp. In the rural (n = 2) and urban (n = 7) control dust samples, the microbial viable counts were >2000 CFU and between 44 CFU and 215 CFU, respectively, and consisted mainly of bacteria. The low proportion of bacteria and the high proportion of stress tolerant, potentially pathogenic fungi in the dust samples from sport facilities may reflect the influence of disinfection on microbial communities. [ABSTRACT FROM AUTHOR]

Viable airborne pathogenic fungi represent a potential health hazard when exposing vulnerable persons in quantities exceeding their resilience. In this study, 284 indoor fungal isolates from a strain collection of indoor fungi were screened for pathogenic potential through the ability to grow in neutral pH at 37 °C and 30 °C. The isolates were collected from 20 locations including 14 problematic and 6 non-problematic ordinary buildings. Out of the screened isolates, 170 isolates were unable to grow at 37 °C, whereas 67 isolates growing at pH 7.2 at 37 °C were considered as potential opportunistic pathogens. Forty-seven isolates growing at 30 °C but not at 37 °C were considered as less likely pathogens. Out of these categories, 33 and 33 strains, respectively, were identified to the species level. The problematic buildings included known opportunistic pathogens: Aspergillus calidoustus, Trichoderma longibrachiatum, Rhizopus arrhizus and Paecilomyces variotii, as well as less likely pathogens: Aspergillus versicolor, Chaetomium cochliodes, Chaetomium globosum and Chaetomium rectangulare. Opportunistic pathogens such as Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger and Aspergillus tubingensis and less likely pathogens such as Aspergillus westerdijkiae, Chaetomium globosum and Dichotomopilus finlandicus were isolated both from ordinary and from problematic buildings. Aspergillus was the dominant, most diverse genus found during screening for potentially pathogenic isolates in the indoor strain collection. Studies on Aspergillus niger and Aspergillus calidodoustus revealed that tolerance to cleaning chemicals may contribute to the adaptation of Aspergillus species to indoor environments. [ABSTRACT FROM AUTHOR]

In this five-month Swedish field study, we examined losses of nutrients from horse manure over time, in order to examine how regularly manure should be cleared from paddocks in order to minimise the risk of nutrient leaching. Small heaps of manure (400 g) were placed in open cylinders outdoors and samples (five replicates) were taken on 12 occasions from December 2020 to May 2021. The samples were analysed for weight, dry matter content and concentrations of total nitrogen (N), ammonium N, total phosphorus (P), water-extractable P (WEP), potassium (K) and carbon (C). There was a fast decline in P and K concentrations and a strong correlation between accumulated precipitation and losses from the manure into the soil. The mean reduction in total-P was 11 mg P kg−1 manure dry weight per mm accumulated precipitation. Manure N was retained in the manure over the five-month period. In conclusion, this study demonstrated high mobility of P and K, indicating a need for strategies for rapid removal of manure from paddocks. Daily removal of manure from paddocks used year-round would, approximately, save 1.7 kg P and 5.5 kg K per horse per year, which could be recycled to replace non-renewable mineral fertilisers. [ABSTRACT FROM AUTHOR]

Moisture excess in buildings constitutes a complex problem affecting indoor air quality, energy consumption and the lifetime of the building envelope. We investigate the effect on moisture transfer in structures as a positive pressure is applied inside the enclosure. It is found that, contrary to established belief, the positive pressure does not induce any negative effects on the structures' moisture content in normally ventilated classrooms, even with high occupancy. Our case study consists of a school building in Finland, subject to temperature and relative humidity measurements after a small (5–7 Pa) positive pressure was realized through ventilation control. We first address analytically the moisture excess generated inside the classrooms for 14 days, using dynamical balance equations that account for both ventilation effects and occupants' moisture release in the environment. It is found that the average moisture excess is very small, largely below 1 g/m3, even for ventilation rates that are half the design value. We also examine the moisture performance of the envelope, by addressing the moisture migration at upper and lower joints of the external walls for both measured and design values of the indoor absolute humidity (AH). A coupled numerical model of diffusion and convection shows that moisture accumulation in the envelope and the according stresses are negligible for any realistic AH values. This result is in agreement with field measurements at the school. In conclusion, it seems that applying a small overpressure in a well-ventilated school building during a standard service period resulted in no accumulation inside the external walls, even at high occupancy and with low ventilation. Remarkably, it slightly dried out the moisture content in structures under actual occupancy conditions. The positive pressure has accordingly no negative effects on moisture performance, and is capable to guarantee a good indoor air quality as well. [ABSTRACT FROM AUTHOR]

Research relating to ecosystem services has increased, partly because of drastic declines in biodiversity in agricultural landscapes. However, the mechanistic linkages between land use, biodiversity and service provision are poorly understood and synthesized. This is particularly true for many ecosystem services provided by mobile organisms such as natural enemies to crop pests. These species are not only influenced by local land use but also by landscape composition at larger spatial scales., We present a conceptual ecological production function framework for predicting land-use impact on biological control of pests by natural enemies. We develop a novel, mechanistic landscape model for biological control of cereal aphids, explicitly accounting for the influence of landscape composition on natural enemies varying in mobility, feeding rates and other life history traits. Finally, we use the model to map biological control services across cereal fields in a Swedish agricultural region with varying landscape complexity., The model predicted that biological control would reduce crop damage by 45-70% and that the biological control effect would be higher in complex landscapes. In a validation with independent data, the model performed well and predicted a significant proportion of biological control variation in cereal fields. However, much variability remains to be explained, and we propose that the model could be improved by refining the mechanistic understanding of predator dynamics and accounting for variation in aphid colonization., We encourage scientists working with biological control to adopt the conceptual framework presented here and to develop production functions for other crop-pest systems. If this kind of ecological production function is combined with production functions for other services, the joint model will be a powerful tool for managing ecosystem services and planning for sustainable agriculture at the landscape scale. [ABSTRACT FROM AUTHOR]

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• Indoor air quality (IAQ) of schools with/without reported IAQ complaints were studied. • Indoor climate questionnaires were used to verify the categorisation of schools. • More cultivable airborne microbes were found in schools with reported IAQ complaints. • Trichoderma was found only in the schools with reported IAQ complaints. • Other IAQ-related measurements were unable to uncover reasons for IAQ complaints. Reported indoor air quality (IAQ) complaints are common even in relatively new or renovated school buildings in Finland. However, detecting the causes for complaints with commonly used indoor air measurements is difficult. This study presents data on perceived and measured IAQ in six comprehensive school buildings in Finland. The aim of this study was to discover the possible differences of perceived and measured IAQ between schools with reported IAQ complaints and schools without reported IAQ complaints. The initial categorisation of schools with ('problematic schools') and without ('comparison schools') complaints was ensured via a validated indoor climate survey and a recently developed online questionnaire, which were completed by 186 teachers and 1268 students from the six schools. IAQ measurements of physical parameters, gaseous pollutants, particulate matter and bioaerosols were conducted in four problematic school buildings (26 classrooms) and two comparison school buildings (12 classrooms). Using air sampling as well as exhaust air filters and classroom settled dust to detect the presence of elevated concentrations of airborne cultivable microbes and pathogenic, toxigenic and mycoparasitic Trichoderma strains were the most indicative methods in distinguishing problematic schools from comparison schools. Other IAQ-related measurements did not detect clear differences between problematic and comparison schools, as the concentration levels were very low. The results indicate that the complaints reported by occupants could have been related to excess moisture or mould problems that had not been found or repaired. Ventilation pressure condition investigations and simultaneous exhaust and supply air filter dust culture should be addressed precisely in future studies. [ABSTRACT FROM AUTHOR]

School environments are a complex entirety where various different exposure factors are related that contribute to the indoor air quality (IAQ) and may affect occupants’ health and well-being. Indoor air questionnaires are useful for collecting information about the occupants’ experiences and perceptions of the indoor air and for evaluating the results of the measures taken. A common way to implement health questionnaires is to ask the respondents to describe symptoms at certain time points, such as weeks or months. The aim of our study was to develop a short and easy online questionnaire to assess symptoms and perceived IAQ. We also aimed to test the usability of the questionnaire in school buildings and assess the differences between the online measurement data (CO2, T, and RH) and the IAQ complaints and symptoms reported by the pupils. A total of 105 teachers and 1268 pupils in 36 classrooms at six schools answered the questionnaires over a two-week period. The participants completed the questionnaire always after the lesson in the studied classroom. We received 719 answers from the teachers and 6322 answers from the pupils. The results demonstrated that the teachers reported more IAQ problems and symptoms than the pupils did. Differences between classrooms were observed in both the IAQ problem and reference schools. The most common significant differences (p-value > 0.05) between the classrooms were among humidity, too cold air, and stuffy air, and among symptoms, dry/sore throat, tiredness, headache, and skin symptoms. Maximum values of CO2 measurements and the highest prevalence of stuffy air were relatively consistent. The testing process demonstrated that such a questionnaire was suitable for adults and children aged at least 12 years. The results of our study suggest that a quick and easy online questionnaire that is completed within a short period may be useful for gathering valuable knowledge about perceived IAQ. It could be used in combination with other indoor environment investigations to produce detailed results and restorative measures. [ABSTRACT FROM AUTHOR]