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35 results on '"Gilard, Françoise"'

3. Photoperiod Affects the Phenotype of Mitochondrial Complex I Mutants

Author

Pétriacq, Pierre, de Bont, Linda, Genestout, Lucie, Hao, Jingfang, Laureau, Constance, Florez-Sarasa, Igor, Rzigui, Touhami, Queval, Guillaume, Gilard, Françoise, Mauve, Caroline, Guérard, Florence, Lamothe-Sibold, Marlène, Marion, Jessica, Fresneau, Chantal, Brown, Spencer, Danon, Antoine, Krieger-Liszkay, Anja, Berthomé, Richard, Ribas-Carbo, Miquel, Tcherkez, Guillaume, Cornic, Gabriel, Pineau, Bernard, Gakière, Bertrand, and De Paepe, Rosine

Published
2017

4. AIF loss deregulates hematopoiesis and reveals different adaptive metabolic responses in bone marrow cells and thymocytes

Author

Cabon, Lauriane, Bertaux, Audrey, Brunelle-Navas, Marie-Noëlle, Nemazanyy, Ivan, Scourzic, Laurianne, Delavallée, Laure, Vela, Laura, Baritaud, Mathieu, Bouchet, Sandrine, Lopez, Cécile, Quang Van, Vu, Garbin, Kevin, Chateau, Danielle, Gilard, Françoise, Sarfati, Marika, Mercher, Thomas, Bernard, Olivier A., and Susin, Santos A.

Published
2018
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10. Study of the genetic and phenotypic variation among wild and cultivated clary sages provides interesting avenues for breeding programs of a perfume, medicinal and aromatic plant

Author

Chalvin, Camille, primary, Drevensek, Stéphanie, additional, Chollet, Christel, additional, Gilard, Françoise, additional, Šolić, Edita M., additional, Dron, Michel, additional, Bendahmane, Abdelhafid, additional, Boualem, Adnane, additional, and Cornille, Amandine, additional

Published
2021
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11. Bradyrhizobium diazoefficiens USDA110 Nodulation of Aeschynomene afraspera Is Associated with Atypical Terminal Bacteroid Differentiation and Suboptimal Symbiotic Efficiency

Author

Nicoud, Quentin, primary, Lamouche, Florian, additional, Chaumeret, Anaïs, additional, Balliau, Thierry, additional, Le Bars, Romain, additional, Bourge, Mickaël, additional, Pierre, Fabienne, additional, Guérard, Florence, additional, Sallet, Erika, additional, Tuffigo, Solenn, additional, Pierre, Olivier, additional, Dessaux, Yves, additional, Gilard, Françoise, additional, Gakière, Bertrand, additional, Nagy, Istvan, additional, Kereszt, Attila, additional, Zivy, Michel, additional, Mergaert, Peter, additional, Gourion, Benjamin, additional, and Alunni, Benoit, additional

Published
2021
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14. The Consequences of a Disruption in Cyto-Nuclear Coadaptation on the Molecular Response to a Nitrate Starvation in Arabidopsis

Author

Chardon, Fabien, Cueff, Gwendal, Delannoy, Etienne, Aubé, Fabien, Lornac, Aurélia, Bedu, Magali, Gilard, Françoise, Pateyron, Stéphanie, Rogniaux, Hélène, Gargaros, Audrey, Mireau, Hakim, Rajjou, Loïc, Martin-Magniette, Marie-Laure, Budar, Francoise, Institut Jean-Pierre Bourgin (IJPB), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), BioInformatique et BioStatistiques (BIBS), Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Mathématiques et Informatique Appliquées (MIA Paris-Saclay), ANR-17-EURE-0007,SPS-GSR,Ecole Universitaire de Recherche de Sciences des Plantes de Paris-Saclay(2017), ANR-12-ADAP-0004,CYTOPHENO,Co-adaptation nucléo-cytoplasmique et phénotypes adaptatifs des plantes(2012), Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre International de Recherche en Infectiologie - UMR (CIRI), Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Mathématiques et Informatique Appliquées (MIA-Paris), and Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-AgroParisTech-Université Paris-Saclay

Subjects
Arabidopsis thaliana, lcsh:Botany, proteome, nutrient stress, food and beverages, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, natural variation, metabolome, transcriptome, Article, lcsh:QK1-989, cytonuclear co adaptation
Abstract

International audience; Mitochondria and chloroplasts are important actors in the plant nutritional efficiency. So, it could be expected that a disruption of the coadaptation between nuclear and organellar genomes impact plant response to nutrient stresses. We addressed this issue using two Arabidopsis accessions, namely Ct-1 and Jea, and their reciprocal cytolines possessing the nuclear genome from one parent and the organellar genomes of the other one. We measured gene expression, and quantified proteins and metabolites under N starvation and non-limiting conditions. We observed a typical response to N starvation at the phenotype and molecular levels. The phenotypical response to N starvation was similar in the cytolines compared to the parents. However, we observed an effect of the disruption of genomic coadaptation at the molecular levels, distinct from the previously described responses to organellar stresses. Strikingly, genes differentially expressed in cytolines compared to parents were mainly repressed in the cytolines. These genes encoded more mitochondrial and nuclear proteins than randomly expected, while N starvation responsive ones were enriched in genes for chloroplast and nuclear proteins. In cytolines, the non-coadapted cytonuclear genomic combination tends to modulate the response to N starvation observed in the parental lines on various biological processes.

Published
2020

16. From Intracellular Bacteria to Differentiated Bacteroids: Transcriptome and Metabolome Analysis in Aeschynomene Nodules Using the Bradyrhizobium sp. Strain ORS285 bclA Mutant

Author

Lamouche, Florian, primary, Chaumeret, Anaïs, additional, Guefrachi, Ibtissem, additional, Barrière, Quentin, additional, Pierre, Olivier, additional, Guérard, Florence, additional, Gilard, Françoise, additional, Giraud, Eric, additional, Dessaux, Yves, additional, Gakière, Bertrand, additional, Timchenko, Tatiana, additional, Kereszt, Attila, additional, Mergaert, Peter, additional, and Alunni, Benoit, additional

Published
2019
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17. Soybean Inoculated With One Bradyrhizobium Strain Isolated at Elevated [CO2] Show an Impaired C and N Metabolism When Grown at Ambient [CO2].

Author

Soba, David, Aranjuelo, Iker, Gakière, Bertrand, Gilard, Françoise, Pérez-López, Usue, Mena-Petite, Amaia, Muñoz-Rueda, Alberto, Lacuesta, Maite, and Sanz-Saez, Alvaro

Subjects
VACCINATION, BRADYRHIZOBIUM, SOYBEAN, METABOLISM, ASPARTIC acid, OXIDATIVE stress
Abstract

Soybean (Glycine max L.) future response to elevated [CO2] has been shown to differ when inoculated with B. japonicum strains isolated at ambient or elevated [CO2]. Plants, inoculated with three Bradyrhizobium strains isolated at different [CO2], were grown in chambers at current and elevated [CO2] (400 vs. 700 ppm). Together with nodule and leaf metabolomic profile, characterization of nodule N-fixation and exchange between organs were tested through 15N2-labeling analysis. Soybeans inoculated with SFJ14-36 strain (isolated at elevated [CO2]) showed a strong metabolic imbalance, at nodule and leaf levels when grown at ambient [CO2], probably due to an insufficient supply of N by nodules, as shown by 15N2-labeling. In nodules, due to shortage of photoassimilate, C may be diverted to aspartic acid instead of malate in order to improve the efficiency of the C source sustaining N2-fixation. In leaves, photorespiration and respiration were boosted at ambient [CO2] in plants inoculated with this strain. Additionally, free phytol, antioxidants, and fatty acid content could be indicate induced senescence due to oxidative stress and lack of nitrogen. Therefore, plants inoculated with Bradyrhizobium strain isolated at elevated [CO2] may have lost their capacity to form effective symbiosis at ambient [CO2] and that was translated at whole plant level through metabolic impairment. [ABSTRACT FROM AUTHOR]

Published
2021
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18. One way to achieve germination: common molecular mechanism induced by ethylene and after-ripening in Sunflower seeds

Author

Xia, Quiong, Saux, Marine, Ponnaiah, Maharajah, Gilard, Françoise, Perreau, François, Huguet, Stéphanie, Balzergue, Sandrine, Langlade, Nicolas, Bailly, Christophe, Meimoun, Patrice, Corbeau, Françoise, and El-Maarouf-Bouteau, Hayat

Subjects
fungi, food and beverages, seed, dormancy, hormones, transcriptomics, metabolomics
Abstract

Dormancy is an adaptive trait that blocks seed germination until the environmental conditions become favorable for subsequent vegetative plant growth. Seed dormancy is defined as the inability to germinate in favorable conditions. Dormancy is alleviated during after-ripening, a dry storage period, during which dormant (D) seeds unable to germinate become non-dormant (ND), able to germinate in a wide range of environmental conditions. The treatment of dormant seeds with ethylene (D/ET) promotes seed germination, and abscisic acid (ABA) treatment reduces non-dormant (ND/ABA) seed germination in sunflowers (Helianthus annuus). Metabolomic and transcriptomic studies have been performed during imbibition to compare germinating seeds (ND and D/ET) and low-germinating seeds (D and ND/ABA). A PCA analysis of the metabolites content showed that imbibition did not trigger a significant change during the first hours (3 and 15 h). The metabolic changes associated with germination capacity occurred at 24 h and were related to hexoses, as their content was higher in ND and D/ET and was reduced by ABA treatment. At the transcriptional level, a large number of genes were altered oppositely in germinating, compared to the low-germinating seeds. The metabolomic and transcriptomic results were integrated in the interpretation of the processes involved in germination. Our results show that ethylene treatment triggers molecular changes comparable to that of after-ripening treatment, concerning sugar metabolism and ABA signaling inhibition.

Published
2018

19. One Way to Achieve Germination: Common Molecular Mechanism Induced by Ethylene and After-Ripening in Sunflower Seeds

Author

Xia, Qiong, Saux, Marine, Ponnaiah, Maharajah, Gilard, Françoise, Perreau, Francois, Huguet, Stéphanie, Balzergue, Sandrine, Langlade, Nicolas, Bailly, Christophe, Meimoun, Patrice, Corbineau, Françoise, El-Maarouf-Bouteau, Hayat, Laboratoire de Biologie du Développement [IBPS] (LBD), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Unité de recherche en génomique végétale (URGV), Institut National de la Recherche Agronomique (INRA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche en Horticulture et Semences (IRHS), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Laboratoire des interactions plantes micro-organismes (LIPM), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), LabEx Saclay Plant Sciences-SPS [ANR-10-LABX-0040-SPS], Laboratoire de Biologie du Développement [Paris] (LBD), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de la Recherche Agronomique (INRA)-Université d'Angers (UA), Laboratoire de Biologie du Développement (LBD), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris-Saclay-Université Paris-Sud - Paris 11 (UP11), Centre National de la Recherche Scientifique (CNRS)-Université d'Évry-Val-d'Essonne (UEVE)-Institut National de la Recherche Agronomique (INRA), AGROCAMPUS OUEST-Institut National de la Recherche Agronomique (INRA)-Université d'Angers (UA), and Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)

Subjects
dormancy, hormones, fungi, food and beverages, Germination, Ethylenes, Plant Dormancy, metabolomics, Article, lcsh:Chemistry, transcriptomics, lcsh:Biology (General), lcsh:QD1-999, Plant Growth Regulators, Gene Expression Regulation, Plant, Seeds, Metabolome, Helianthus, Transcriptome, lcsh:QH301-705.5, seed, [SDV.BDD]Life Sciences [q-bio]/Development Biology
Abstract

International audience; Dormancy is an adaptive trait that blocks seed germination until the environmental conditions become favorable for subsequent vegetative plant growth. Seed dormancy is defined as the inability to germinate in favorable conditions. Dormancy is alleviated during after-ripening, a dry storage period, during which dormant (D) seeds unable to germinate become non-dormant (ND), able to germinate in a wide range of environmental conditions. The treatment of dormant seeds with ethylene (D/ET) promotes seed germination, and abscisic acid (ABA) treatment reduces non-dormant (ND/ABA) seed germination in sunflowers (Helianthus annuus). Metabolomic and transcriptomic studies have been performed during imbibition to compare germinating seeds (ND and D/ET) and low-germinating seeds (D and ND/ABA). A PCA analysis of the metabolites content showed that imbibition did not trigger a significant change during the first hours (3 and 15 h). The metabolic changes associated with germination capacity occurred at 24 h and were related to hexoses, as their content was higher in ND and D/ET and was reduced by ABA treatment. At the transcriptional level, a large number of genes were altered oppositely in germinating, compared to the low-germinating seeds. The metabolomic and transcriptomic results were integrated in the interpretation of the processes involved in germination. Our results show that ethylene treatment triggers molecular changes comparable to that of after-ripening treatment, concerning sugar metabolism and ABA signaling inhibition.

Published
2018

22. Metabolic profiling of two maize (Zea mays L.) inbred lines inoculated with the nitrogen fixing plant-interacting bacteria Herbaspirillum seropedicae and Azospirillum brasilense

Author

Brusamarello-Santos, Liziane Cristina, Gilard, Françoise, BRULE, Lenaïg, Quilleré, Isabelle, Gourion, Benjamin, Ratet, Pascal, Maltempi De Souza, Emanuel, Lea, Peter, Hirel, Bertrand, Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Department of Biochemistry and Molecular Biology, Mayo Clinic, Centre National de la Recherche Scientifique (CNRS), AgroParisTech, Université Paris-Saclay, Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Institut National de la Recherche Agronomique (INRA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris-Saclay-Université Paris-Sud - Paris 11 (UP11), Lancaster Environment Centre, Soil and Ecosystem Ecology Laboratory, National Institute of Science and Technology-Biological Nitrogen Fixation (INCT-FBN) through the Brazilian Research Council (CNPq/MCT) Glenda Sem Fronteiras Program, Brazil, Empresa Brasileira de Pesquisa Agropecuaria (BR), Institut National de la Recherche Agronomique, Centre National de la Recherche Scientifique, National Institute of Science and Technology-Biological Nitrogen Fixation (INCT-FBN) through the Brazilian Research Council (CNPq/MCT), Ciencia Sem Fronteiras Program, Brazil, Universidade Federal do Paraná (UFPR), Laboratoire des symbioses tropicales et méditerranéennes (UMR LSTM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut des sciences du végétal (ISV), Lancaster University, Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS)-Université d'Évry-Val-d'Essonne (UEVE)-Institut National de la Recherche Agronomique (INRA), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Nitrogen-Fixing Bacteria, Leaves, Herbaspirillum, Nitrogen, [SDV]Life Sciences [q-bio], Carbohydrates, lcsh:Medicine, Azospirillum brasilense, Plant Science, Diazo Compounds, Research and Analysis Methods, Plant Roots, Zea mays, Biochemistry, Model Organisms, Glucose Metabolism, Plant and Algal Models, Metabolites, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Grasses, Fertilizers, lcsh:Science, Plant Biochemistry, Organic Compounds, Plant Anatomy, Organic Chemistry, lcsh:R, Organisms, Chemical Compounds, Biology and Life Sciences, Agriculture, Plants, Maize, Chemistry, Metabolism, Experimental Organism Systems, Physical Sciences, Carbohydrate Metabolism, lcsh:Q, Agrochemicals, Research Article
Abstract

International audience; Maize roots can be colonized by free-living atmospheric nitrogen (N2)-fixing bacteria (diazotrophs). However, the agronomic potential of non-symbiotic N2-fixation in such an economically important species as maize, has still not been fully exploited. A preliminary approach to improve our understanding of the mechanisms controlling the establishment of such N2-fixing associations has been developed, using two maize inbred lines exhibiting different physiological characteristics. The bacterial-plant interaction has been characterized by means of a metabolomic approach. Two established model strains of Nif+ diazotrophic bacteria, Herbaspirillum seropedicae and Azospirillum brasilense and their Nif- couterparts defficient in nitrogenase activity, were used to evaluate the impact of the bacterial inoculation and of N2 fixation on the root and leaf metabolic profiles. The two N2-fixing bacteria have been used to inoculate two genetically distant maize lines (FV252 and FV2), already characterized for their contrasting physiological properties. Using a well-controlled gnotobiotic experimental system that allows inoculation of maize plants with the two diazotrophs in a N-free medium, we demonstrated that both maize lines were efficiently colonized by the two bacterial species. We also showed that in the early stages of plant development, both bacterial strains were able to reduce acetylene, suggesting that they contain functional nitrogenase activity and are able to efficiently fix atmospheric N2 (Fix+). The metabolomic approach allowed the identification of metabolites in the two maize lines that were representative of the N2 fixing plant-bacterial interaction, these included mannitol and to a lesser extend trehalose and isocitrate. Whilst other metabolites such as asparagine, although only exhibiting a small increase in maize roots following bacterial infection, were specific for the two Fix+ bacterial strains, in comparison to their Fix- counterparts. Moreover, a number of metabolites exhibited a maize-genotype specific pattern of accumulation, suggesting that the highly diverse maize genetic resources could be further exploited in terms of beneficial plant-bacterial interactions for optimizing maize growth, with reduced N fertilization inputs.

Published
2017

23. Photoperiod Affects the Phenotype of Mitochondrial Complex I Mutants1[OPEN]

Author

Pétriacq, Pierre, de Bont, Linda, Genestout, Lucie, Hao, Jingfang, Laureau, Constance, Florez-Sarasa, Igor, Rzigui, Touhami, Queval, Guillaume, Gilard, Françoise, Mauve, Caroline, Guérard, Florence, Lamothe-Sibold, Marlène, Marion, Jessica, Fresneau, Chantal, Brown, Spencer, Danon, Antoine, Krieger-Liszkay, Anja, Berthomé, Richard, Ribas-Carbo, Miquel, Tcherkez, Guillaume, Cornic, Gabriel, Pineau, Bernard, Gakière, Bertrand, and De Paepe, Rosine

Subjects
endocrine system, Electron Transport Complex I, Light, Arabidopsis Proteins, Nitrogen, Photoperiod, Arabidopsis, Articles, Antioxidants, Carbon, Plant Leaves, Gene Expression Regulation, Plant, Mutation, hormones, hormone substitutes, and hormone antagonists
Abstract

Plant mutants for genes encoding subunits of mitochondrial complex I (CI; NADH:ubiquinone oxidoreductase), the first enzyme of the respiratory chain, display various phenotypes depending on growth conditions. Here, we examined the impact of photoperiod, a major environmental factor controlling plant development, on two Arabidopsis (Arabidopsis thaliana) CI mutants: a new insertion mutant interrupted in both ndufs8.1 and ndufs8.2 genes encoding the NDUFS8 subunit and the previously characterized ndufs4 CI mutant. In the long day (LD) condition, both ndufs8.1 and ndufs8.2 single mutants were indistinguishable from Columbia-0 at phenotypic and biochemical levels, whereas the ndufs8.1 ndufs8.2 double mutant was devoid of detectable holo-CI assembly/activity, showed higher alternative oxidase content/activity, and displayed a growth retardation phenotype similar to that of the ndufs4 mutant. Although growth was more affected in ndufs4 than in ndufs8.1 ndufs8.2 under the short day (SD) condition, both mutants displayed a similar impairment of growth acceleration after transfer to LD compared with the wild type. Untargeted and targeted metabolomics showed that overall metabolism was less responsive to the SD-to-LD transition in mutants than in the wild type. The typical LD acclimation of carbon and nitrogen assimilation as well as redox-related parameters was not observed in ndufs8.1 ndufs8 Similarly, NAD(H) content, which was higher in the SD condition in both mutants than in Columbia-0, did not adjust under LD We propose that altered redox homeostasis and NAD(H) content/redox state control the phenotype of CI mutants and photoperiod acclimation in Arabidopsis.

Published
2016

25. Photoperiod Affects the Phenotype of Mitochondrial Complex I Mutants

Author

Pétriacq, Pierre, primary, de Bont, Linda, additional, Genestout, Lucie, additional, Hao, Jingfang, additional, Laureau, Constance, additional, Florez-Sarasa, Igor, additional, Rzigui, Touhami, additional, Queval, Guillaume, additional, Gilard, Françoise, additional, Mauve, Caroline, additional, Guérard, Florence, additional, Lamothe-Sibold, Marlène, additional, Marion, Jessica, additional, Fresneau, Chantal, additional, Brown, Spencer, additional, Danon, Antoine, additional, Krieger-Liszkay, Anja, additional, Berthomé, Richard, additional, Ribas-Carbo, Miquel, additional, Tcherkez, Guillaume, additional, Cornic, Gabriel, additional, Pineau, Bernard, additional, Gakière, Bertrand, additional, and De Paepe, Rosine, additional

Published
2016
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26. GOLLUM [FeFe]-hydrogenase-like proteins are essential for plant development in normoxic conditions and modulate energy metabolism

Author

Mondy, Samuel, Lenglet, Aurore, Cosson, Viviane, Pelletier, Sandra, Pateyron, Stephanie, Gilard, Françoise, Scholte, Marije, Brocard, Lysiane, Couzigou, Jean-Malo, Tcherkez, Guillaume, Péan, Michel, Ratet, Pascal, Institut des sciences du végétal (ISV), Centre National de la Recherche Scientifique (CNRS), Unité de recherche en génomique végétale (URGV), Institut National de la Recherche Agronomique (INRA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Université Paris-Sud - Paris 11 (UP11), Aix Marseille Université (AMU), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), French MENRT, and French ANR SEPTANTE programme

Subjects
iron-sulfur protein, hypoxia, IOP, Arabidopsis, HIF-1, HIF-1α, arabidopsis-thaliana, NAR1, Oxygen, stress, alternative oxidase, Medicago, insertional mutagenesis, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Warburg effect, gene, oxygen, atmospheric oxygen, respiration, legume medicago-truncatula
Abstract

International audience; [FeFe]-hydrogenase-like genes encode [Fe4S4]-containing proteins that are ubiquitous in eukaryotic cells. In humans, iron-only hydrogenase-like protein 1 (IOP1) represses hypoxia inducible factor-1 subunit (HIF1-) at normal atmospheric partial O-2 pressure (normoxia, 21kPa O-2). In yeasts, the nar1 mutant cannot grow at 21kPa O-2,O- but can develop at a lower O-2 pressure (2kPa O-2). We show here that plant [FeFe]-hydrogenase-like GOLLUM genes are essential for plant development and cell cycle progression. The mutant phenotypes of these plants are seen in normoxic conditions, but not under conditions of mild hypoxia (5kPa O-2). Transcriptomic and metabolomic experiments showed that the mutation enhances the expression of some hypoxia-induced genes under normal atmospheric O-2 conditions and changes the cellular content of metabolites related to energy metabolism. In conclusion, [FeFe]-hydrogenase-like proteins play a central role in eukaryotes including the adaptation of plants to the ambient O-2 partial pressure.

Published
2014

30. In situ proteo-metabolomics reveals metabolite secretion by the acid mine drainage bio-indicator, Euglena mutabilis

Author

Halter, David, primary, Goulhen-Chollet, Florence, additional, Gallien, Sébastien, additional, Casiot, Corinne, additional, Hamelin, Jérôme, additional, Gilard, Françoise, additional, Heintz, Dimitri, additional, Schaeffer, Christine, additional, Carapito, Christine, additional, Van Dorsselaer, Alain, additional, Tcherkez, Guillaume, additional, Arsène-Ploetze, Florence, additional, and Bertin, Philippe N, additional

Published
2012
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32. Carbon-Flux Distribution within Streptomyces coelicolor Metabolism: A Comparison between the Actinorhodin-Producing Strain M145 and Its Non-Producing Derivative M1146.

Author

Coze, Fabien, Gilard, Françoise, Tcherkez, Guillaume, Virolle, Marie-Joëlle, and Guyonvarch, Armel

Subjects
*STREPTOMYCES coelicolor, *METABOLIC flux analysis, *METABOLISM, *ACTINORHODIN, *GENETIC engineering, *PYRUVATE carboxylase, *ANTIBIOTICS
Abstract

Metabolic Flux Analysis is now viewed as essential to elucidate the metabolic pattern of cells and to design appropriate genetic engineering strategies to improve strain performance and production processes. Here, we investigated carbon flux distribution in two Streptomyces coelicolor A3 (2) strains: the wild type M145 and its derivative mutant M1146, in which gene clusters encoding the four main antibiotic biosynthetic pathways were deleted. Metabolic Flux Analysis and 13C-labeling allowed us to reconstruct a flux map under steady-state conditions for both strains. The mutant strain M1146 showed a higher growth rate, a higher flux through the pentose phosphate pathway and a higher flux through the anaplerotic phosphoenolpyruvate carboxylase. In that strain, glucose uptake and the flux through the Krebs cycle were lower than in M145. The enhanced flux through the pentose phosphate pathway in M1146 is thought to generate NADPH enough to face higher needs for biomass biosynthesis and other processes. In both strains, the production of NADPH was higher than NADPH needs, suggesting a key role for nicotinamide nucleotide transhydrogenase for redox homeostasis. ATP production is also likely to exceed metabolic ATP needs, indicating that ATP consumption for maintenance is substantial.Our results further suggest a possible competition between actinorhodin and triacylglycerol biosynthetic pathways for their common precursor, acetyl-CoA. These findings may be instrumental in developing new strategies exploiting S. coelicolor as a platform for the production of bio-based products of industrial interest. [ABSTRACT FROM AUTHOR]

Published
2013
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33. A vacuolar hexose transport is required for xylem development in the inflorescence stem.

Author

Aubry E, Hoffmann B, Vilaine F, Gilard F, Klemens PAW, Guérard F, Gakière B, Neuhaus HE, Bellini C, Dinant S, and Le Hir R

Subjects
Arabidopsis metabolism, Biological Transport genetics, Genetic Variation, Genotype, Inflorescence metabolism, Mutation, Vacuoles physiology, Xylem metabolism, Arabidopsis genetics, Arabidopsis growth & development, Hexoses metabolism, Inflorescence genetics, Inflorescence growth & development, Xylem genetics, Xylem growth & development
Abstract

In Angiosperms, the development of the vascular system is controlled by a complex network of transcription factors. However, how nutrient availability in the vascular cells affects their development remains to be addressed. At the cellular level, cytosolic sugar availability is regulated mainly by sugar exchanges at the tonoplast through active and/or facilitated transport. In Arabidopsis (Arabidopsis thaliana), among the genes encoding tonoplastic transporters, SUGAR WILL EVENTUALLY BE EXPORTED TRANSPORTER 16 (SWEET16) and SWEET17 expression has been previously detected in the vascular system. Here, using a reverse genetics approach, we propose that sugar exchanges at the tonoplast, regulated by SWEET16, are important for xylem cell division as revealed in particular by the decreased number of xylem cells in the swt16 mutant and the accumulation of SWEET16 at the procambium-xylem boundary. In addition, we demonstrate that transport of hexoses mediated by SWEET16 and/or SWEET17 is required to sustain the formation of the xylem secondary cell wall. This result is in line with a defect in the xylem cell wall composition as measured by Fourier-transformed infrared spectroscopy in the swt16swt17 double mutant and by upregulation of several genes involved in secondary cell wall synthesis. Our work therefore supports a model in which xylem development partially depends on the exchange of hexoses at the tonoplast of xylem-forming cells., (© American Society of Plant Biologists 2021. All rights reserved. For permissions, please email: journals.permissions@oup.com.)

Published
2022
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34. Soybean Inoculated With One Bradyrhizobium Strain Isolated at Elevated [CO 2 ] Show an Impaired C and N Metabolism When Grown at Ambient [CO 2 ].

Author

Soba D, Aranjuelo I, Gakière B, Gilard F, Pérez-López U, Mena-Petite A, Muñoz-Rueda A, Lacuesta M, and Sanz-Saez A

Abstract

Soybean ( Glycine max L.) future response to elevated [CO 2 ] has been shown to differ when inoculated with B. japonicum strains isolated at ambient or elevated [CO 2 ]. Plants, inoculated with three Bradyrhizobium strains isolated at different [CO 2 ], were grown in chambers at current and elevated [CO 2 ] (400 vs. 700 ppm). Together with nodule and leaf metabolomic profile, characterization of nodule N-fixation and exchange between organs were tested through 15 N 2 -labeling analysis. Soybeans inoculated with SFJ14-36 strain (isolated at elevated [CO 2 ]) showed a strong metabolic imbalance, at nodule and leaf levels when grown at ambient [CO 2 ], probably due to an insufficient supply of N by nodules, as shown by 15 N 2 -labeling. In nodules, due to shortage of photoassimilate, C may be diverted to aspartic acid instead of malate in order to improve the efficiency of the C source sustaining N 2 -fixation. In leaves, photorespiration and respiration were boosted at ambient [CO 2 ] in plants inoculated with this strain. Additionally, free phytol, antioxidants, and fatty acid content could be indicate induced senescence due to oxidative stress and lack of nitrogen. Therefore, plants inoculated with Bradyrhizobium strain isolated at elevated [CO 2 ] may have lost their capacity to form effective symbiosis at ambient [CO 2 ] and that was translated at whole plant level through metabolic impairment., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Soba, Aranjuelo, Gakière, Gilard, Pérez-López, Mena-Petite, Muñoz-Rueda, Lacuesta and Sanz-Saez.)

Published
2021
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35. One Way to Achieve Germination: Common Molecular Mechanism Induced by Ethylene and After-Ripening in Sunflower Seeds.

Author

Xia Q, Saux M, Ponnaiah M, Gilard F, Perreau F, Huguet S, Balzergue S, Langlade N, Bailly C, Meimoun P, Corbineau F, and El-Maarouf-Bouteau H

Subjects
Gene Expression Regulation, Plant, Helianthus genetics, Helianthus metabolism, Metabolome, Plant Dormancy, Seeds genetics, Seeds metabolism, Transcriptome, Ethylenes metabolism, Germination, Helianthus growth & development, Plant Growth Regulators metabolism, Seeds growth & development
Abstract

Dormancy is an adaptive trait that blocks seed germination until the environmental conditions become favorable for subsequent vegetative plant growth. Seed dormancy is defined as the inability to germinate in favorable conditions. Dormancy is alleviated during after-ripening, a dry storage period, during which dormant (D) seeds unable to germinate become non-dormant (ND), able to germinate in a wide range of environmental conditions. The treatment of dormant seeds with ethylene (D/ET) promotes seed germination, and abscisic acid (ABA) treatment reduces non-dormant (ND/ABA) seed germination in sunflowers ( Helianthus annuus ). Metabolomic and transcriptomic studies have been performed during imbibition to compare germinating seeds (ND and D/ET) and low-germinating seeds (D and ND/ABA). A PCA analysis of the metabolites content showed that imbibition did not trigger a significant change during the first hours (3 and 15 h). The metabolic changes associated with germination capacity occurred at 24 h and were related to hexoses, as their content was higher in ND and D/ET and was reduced by ABA treatment. At the transcriptional level, a large number of genes were altered oppositely in germinating, compared to the low-germinating seeds. The metabolomic and transcriptomic results were integrated in the interpretation of the processes involved in germination. Our results show that ethylene treatment triggers molecular changes comparable to that of after-ripening treatment, concerning sugar metabolism and ABA signaling inhibition.

Published
2018
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