1. Altered Polarization, Morphology, and Impaired Innate Immunity Germane to Resident Peritoneal Macrophages in Mice with Long-Term Type 2 Diabetes.
- Author
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Hui-Fang Liu, Hui-Jie Zhang, Qi-Xian Hu, Xiao-Yan Liu, Zhi-Quan Wang, Jia-Yan Fan, Ming Zhan, and Feng-Ling Chen
- Subjects
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MICROSCOPY , *ANIMAL experimentation , *CELL physiology , *FLOW cytometry , *IMMUNITY , *MACROPHAGES , *MICE , *TYPE 2 diabetes , *PERITONEUM , *RESEARCH funding , *T-test (Statistics) , *DESCRIPTIVE statistics - Abstract
Type 2 diabetes (T2D) is associated with perturbed innate immunity. Macrophages, bridging innate immunity and metabolic disturbances, play important roles in controlling immune homeostasis. However, the effect of long-term diabetic milieu (DM) on the functions and phenotypes of macrophages is still not clear. In this study, we used resident peritoneal macrophages (RPMs) from 5-month-old db/db mice to investigate the changes of macrophages. It was found that RPMs in db/db mice significantly reduced phagocytosis and adhesion capacity. After standardization with body weight, the number of F4/80+ RPMs markedly reduced in db/db mice, and, furthermore, the macrophages skewed to M2-polarizated macrophages. The results of morphology found that the RPMs shape of db/db mice was nearly round, but the RPMs shape of control mice was spindle-shaped and irregular. In this study, we found the cell numbers, morphology, and innate immunity functions of RPMs in 5-month-old type 2 diabetic mice (db/db mice) obtained by abdominal cavity lavage were significantly altered. Importantly, we also found the remarkably increased M2-RPMs in diabetic mice for the first time. [ABSTRACT FROM AUTHOR]
- Published
- 2012
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