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This study aims to explore the determinants of preschool choice among middle-income parents in Shanghai, the city with the most adequate and diversified preschool services in China. We administer an online survey to 333 Shanghai middle-income parents and find that preschool quality, children's development, and parents' needs constitute the determinants of preschool choice. Furthermore, the parents are primarily concerned about program quality and the development of children's self-care abilities. Among them, 226 parents are very satisfied with the kindergarten they chose, 99 parents are satisfied, eight are generally satisfied, and none reports feeling dissatisfied. There are significant effects of preschool type, parental jobs, and parental education level on parental satisfaction. Those emerging middle-income parents can only send their children to second-level preschools, which are relatively below their expectations, they tended to have lower satisfaction. These findings imply that the educational authorities should adjust their policymaking to meet the varying needs of middle-income families in rapidly developing cities. Highlights: Preschool quality, children's development, and parents' needs are the main determinants of preschool choice. Middle-income parents' primary concerns are program quality and the development of children's self-care abilities. Three-way ANOVA showed that parental satisfaction differed significantly by preschool type, parental job, and educational level. [ABSTRACT FROM AUTHOR]

One novel ascomycetous yeast strain TF5-16-2 was isolated from water samples of Tuofengling crater lake located in Da Hinggan Ling Mountain, in the Inner Mongolia province of China. Morphological, physiological characteristics, as well as phylogenetic analyses of D1/D2 domains of the large subunit rRNA (LSU), ITS region, small subunit rRNA (SSU), and elongation factor-1α (EF-1α) were performed and finally confirmed the phylogenetic placement of strain TF5-16-2 in the genus Wickerhamomyces. Sequences analysis revealed that strain TF5-16-2 differed from its most closely related phylogenetic neighbors 'Candida' silvicultrix CBS 6269T and Wickerhamomyces anomalus CBS 5759T by 8.0% (including 2.3% gaps), 8.5% (including 2.4% gaps) divergences in D1/D2 domains of LSU, and 11% (including 4.3% gaps) and 13% (including 4.4% gaps) divergences in ITS region, respectively. As the considerable sequence divergence and distinguishable physiological characteristics, strain TF5-16-2 was proposed as a new species of the genus Wickerhamomyces, with the name Wickerhamomyces kurtzmanii sp. nov. (holotype = CGMCC 2.5597, Mycobank number is MB829959). [ABSTRACT FROM AUTHOR]

This research aims to explore detailed spatial distribution characteristics, identify key factors weight, and establish an accurate susceptibility regionalization model of coseismic landslides by the Mw 6.6 Iburi, Japan, earthquake sequence of 6 September 2018. Based on the remote sensing interpretation database, 5,977 individual coseismic landslides were delineated, which occupies an area of about 15.26 km2. The relationship between eight key factors and spatial distribution of coseismic landslides were precisely analyzed by the landslide area density (LAD) and landslide point density (LPD) curves. In order to obtain the weight of eight key factors more accurately, this paper compares the similarities and differences of the two curves in the earthquakes with similar magnitude, which was included the Lushan and Jiuzhaigou earthquake in China. According to the weight of eight key factors, the improved weight of evidence method was used to produce the susceptibility regionalization map of coseismic landslides in the Iburi earthquake. The results show that the improved weight of evidence method has better precision and can provide a scientific coseismic landslides susceptibility regionalization map for disaster prevention and mitigation in the Iburi earthquake. [ABSTRACT FROM AUTHOR]

Background: Studies have identified numerous factors that may affect the sleep quality and quality of life (QOL) in outpatients with schizophrenia. However, the clinically stable inpatients who represent a large proportion of the population with schizophrenia in China have not received enough attention. The present study was performed to explore the sociodemographic and clinical correlates of sleep disturbance and QOL in clinically stable inpatients with schizophrenia in rural China.Methods: A cross-sectional study was designed, and 207 clinically stable inpatients with schizophrenia were selected from Chifeng Anding Hospital, located in Inner Mongolia Autonomous Region, in northern China. All subjects were interviewed by the same investigator using standardized assessment instruments. QOL and sleep disturbance were measured using the Schizophrenia Quality of Life Scale (SQLS) and Pittsburgh Sleep Quality Index (PSQI), respectively. Univariate and multiple regression analyses were used to identify the factors influencing sleep disturbance and QOL. Antipsychotics taken by individuals were converted into olanzapine equivalent doses as the main confounding factor to be controlled.Results: The prevalence of sleep disturbance was 58%, and sleep disturbance was significantly associated with depression (OR 1.33, 95% CI 1.17-1.52) and coping mechanisms (OR 0.95, 95% CI 0.91-0.98). We observed large differences between the sexes: the QOL of male inpatients with schizophrenia was substantially better than that of female inpatients, with a standard coefficient of 0.19 ± 1.62. Other factors related to QOL were depression (0.42 ± 0.30), hope (- 0.21 ± 0.19), general psychopathology symptoms (0.21 ± 0.24) and personal and social performance (- 0.12 ± 0.07).Conclusions: The depressive symptoms of inpatients with schizophrenia should receive more attention. More targeted interventions, such as the early identification and treatment of depression, should be promptly administered to improve the patient's hospitalization experience. [ABSTRACT FROM AUTHOR]

Hourly mapping by a Geostationary Ocean Color Imager was used to reveal the spatial pattern and tidal variation of total suspended solids (TSS) over the Yangtze Bank in the Yellow and East China Seas during the winter. The TSS form a tongue-shaped structure, which decreases further offshore in a stepwise manner. The stepwise change is separated by two fronts of TSS, which are located near the 20-m and 50-m isobaths. The tidal variation of TSS concentration during the study period is evident and can be divided into three stages: decay, maintenance, and growth. Compared with the relatively stationary TSS during the maintenance stage, drastic changes exist during the decay and growth stages. In terms of tide-induced mixing, the dynamic analysis shows that both the topography and the tidal currents play an important role in the spatio-temporal variation of TSS during the tidal period. In particular, spatial distribution is primarily determined by the topography, whereas the temporal variations in tidal scale are determined by the tidal currents. [ABSTRACT FROM AUTHOR]

Rice black-streaked dwarf virus (RBSDV) is an economically important virus that causes maize rough dwarf disease and rice black-streaked dwarf disease in East Asia. To study RBSDV variation and recombination, we examined the segment 9 (S9) sequences of 49 RBSDV isolates from maize and rice in China. Three S9 recombinants were detected in Baoding, Jinan, and Jining, China. Phylogenetic analysis showed that Chinese RBSDV isolates could be classified into two groups based on their S9 sequences, regardless of host or geographical origin. Further analysis suggested that S9 has undergone negative and purifying selection. [ABSTRACT FROM AUTHOR]

The COVID-19 pandemic across Chinese mainland was gradually stabilized at a low level with sporadic outbreaks, before the emergence of Omicron variant. Apart from non-pharmacological interventions (NPIs), COVID-19 vaccine has also been implemented to prevent and control the pandemic since early 2021. Although many aspects have been focused, the change of the spatiotemporal distribution of COVID-19 epidemic across Chinese mainland responding to the change of prevention and control measures were less concerned. Here, we collected the confirmed case data (including domestic cases and overseas imported cases) across Chinese mainland during both 2020/04–2020/08 and 2021/04–2021/08, and then conducted a preliminary data comparison on the spatiotemporal distribution of confirmed cases during the identical period between the two years. Distribution patterns were evaluated both qualitatively by classification method and quantitatively through employing coefficient of variation. Results revealed significant differences in the homogeneity of spatiotemporal distributions of imported or domestic cases between the two years, indicating that the important effect of the adjustment of prevention and control measures on the epidemic evolution. The findings here enriched our practical experience of COVID-19 prevention and control. And, the collected data here might be helpful for improving or verifying spatiotemporally dynamic models of infectious diseases. [ABSTRACT FROM AUTHOR]

Strawberry vein banding virus (SVBV) is one of the serious viral pathogens infecting strawberry worldwide. To understand the molecular characterization of SVBV from China, complete genome sequences of sixteen SVBV isolates were cloned and sequenced. Sequence comparison showed they shared high nucleotide sequence identity (93.6–99.5%) with isolates from China and Japan (96.6–98.4%), while relatively low identity with the isolates from Canada (91.9–93.7%) and USA (85.5–85.9%). Phylogenetic analyses based on the complete genome sequence or coat protein (CP) gene showed the SVBV isolates clustered into three clades correlated with geographic distribution. Recombination analyses identified 13 recombinants and 21 recombinant events, indicating frequent and multiple recombinations in SVBV evolution. Furthermore, a sensitive loop-mediated isothermal amplification (LAMP) method was developed for rapid detection of SVBV isolates, which could be especially suitable for seedling propagation, virus-free culture and routine diagnostics in field investigation. This study offers new understanding of the molecular evolution and may help to improve the management of SVBV. [ABSTRACT FROM AUTHOR]

A bacterial strain, Gram staining negative, aerobic, long rod, motile bacterium with flagellum, designated strain YIM 98829T, was isolated from the Aiding Lake in Xinjiang province, North-West China. The isolate produced oval subterminal endospores in swollen sporangia. The predominant menaquinone was MK-7. The cell wall peptidoglycan contained ornithine, serine, aspartic acid, glutamic acid, and alanine, while diaminopimelic acid could not be detected. The major whole-cell sugars contained xylose, glucose, galactose, and mannose. Diphosphatidylglycerol, phosphatidylglycerol, one unknown phospholipid, and two unidentified aminophospholipids were part of the polar lipid profile. Iso-C15:0 and anteiso-C15:0 were the major fatty acids. The DNA G + C content of the type strain was 38.0 mol%. Phylogenetic analysis indicated that the isolate belongs to the genus Alkalibacillus. However, it differed from its closest relatives, A. haloalkaliphilus DSM 5271T (97.04%), A. filiformis 4AGT (96.99%), and A. silvisoli BM2T (96.95%) in some physiological characteristics. DNA–DNA hybridization result indicated low levels of relatedness between strain YIM 98829T and A. haloalkaliphilus JCM 12303T (16.9%). On the basis of physiological, phenotypic, and chemotaxonomic data, strain YIM 98829T represents a novel species of genus Alkalibacillus, for which the name Alkalibacillus aidingensis sp. nov. is proposed. The type strain is YIM 98829T (= NBRC 114103T = CGMCC 1.17260T = DSM 112470T). [ABSTRACT FROM AUTHOR]

Low temperatures are important for the production of spring maize in northern China. While the low-temperature tolerance of maize seeds can be improved by coating them, this can result in environmental pollution, high costs, and instability. Therefore, identifying new varieties of maize is the most effective method of improving the ability of maize crops to withstand low temperatures. In this experiment, four low-temperature tolerant maize inbred lines (DNF266, Zhong 451, B73, Dan 340) and four low-temperature sensitive maize inbred lines (Luyuan 92, Ji 853, Huangzaosi, Si 144) were selected from the northern spring maize area. The griffing double-row hybridization design method was used to prepare 28 hybrid combinations. After analysing the general combining ability and the special combining ability of each combination, we found that the indoor low-temperature index and low-temperature seedings were extremely similar for all combinations. The hybridization and parental inbred lines were subjected to low-temperature treatments under both laboratory conditions and in the field. Several low-temperature indexes were assessed to identify what results could be produced from combining them. Under both treatments, the inbred line DNF266 had a higher general combining ability and the Zhong 451 × Dan 340 hybrid combination had a higher special combining ability. These results provide technical support for breeding new low-temperature varieties of maize. [ABSTRACT FROM AUTHOR]

The rice cultivar Zhejing 22 has been transformed to carry the Bacillus thuringiensis Cry1A(b) gene in 2007. Although the cultivation of this line (referred to as Bt-ZJ22) is yet to be authorized, it is likely to be approved in China in the near future. The transgene-host 3' integration junction was isolated using thermal asymmetric interlaced PCR, and its sequence was used to design PCR primers and an appropriate TaqMan probe for a real-time PCR quantitative assay of the transgene. The limit of detection for a standard PCR assay was estimated at ten gene copies, while that for the real-time PCR assay was 5-10 copies. The latter assay was tested on samples of rice DNA with either 5, 3, 1, or 0.5% Bt-ZJ22; this produced estimates for the relative content of Bt-ZJ22 of, respectively, 5.3, 3.2, 0.97, and 0.48%. The precision of these estimates demonstrated that this real-time PCR assay should be suitable for the detection and monitoring of this transgenic event. [ABSTRACT FROM AUTHOR]