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Animal testing for cosmetic ingredients and final products has been banned in Europe and is gaining legal force worldwide. However, the need for reliable testing methodologies remains for safety assessment of cosmetic ingredients. While new approach methodologies exist for many toxicological endpoints, some complex ones lack appropriate testing methods. Microphysiological systems (MPSs) have emerged as a promising tool to address this gap in pre-clinical testing, offering higher predictivity compared to animal models due to the phylogenetic distance between humans and animals. Moreover, they provide a more physiological approach than traditional in vitro testing by mimicking interconnections between different culture compartments as seen in complex organisms. This study presents a three-organ microfluidic MPS comprising skin, liver, and intestine equivalents. Combining this model with gene expression analysis, we evaluated toxicological endpoints of chemicals, demonstrating its potential for diverse applications. Our findings highlight the MPS model as a reliable and ethical method to be applied in an integrated approach for safety assessment in the cosmetic industry. It offers a promising strategy to evaluate toxicological endpoints for cosmetic ingredients and other chemicals, supporting the elimination of animal testing while ensuring consumer safety. [ABSTRACT FROM AUTHOR]

Since many decades, gamma-valerolactone (GVL) is known to be an excellent solvent, which, today, can be made of sustainable materials. Although numerous publications praise it as very green and versatile, GVL has never reached the level of a large-scale industrial product. Even relevant toxicity and biodegradability data are missing. In this paper, we present such data. Further, we make a detailed study, based on Hansen solubility parameters (HSP) and COSMO-RS calculations to identify harmful solvents that could be potentially replaced by GVL and to propose a variety of further applications of this substance. Toxicity tests with aquatic plants, bacteria, invertebrates, and a vertebrate cell line indicated a low acute toxicity of GVL towards aquatic organisms. Additionally, GVL was shown to be readily biodegradable, which further strengthens its potential as a "green" solvent. Accurate HSP of GVL were proposed and used in addition to COSMO-RS calculations to compare its solvent properties with the ones of classical, organic solvents. A similarity of GVL with several aprotic, highly dipolar solvents, such as the reprotoxic compounds N-methyl-2-pyrrolidone (NMP) and dimethylformamide (DMF), was found. Based on the results, GVL was concluded to be of interest as a green solvent in the manufacture of certain polymers or pharmaceuticals, as a cleaning agent in various paint and coating formulations, as well as a solubiliser in cosmetics, pharmaceuticals, or agrochemicals. Together with a very significant drop of its price, because of an intended large-scale production in Europe, the presented data should hopefully lead to the boost in applications that this green solvent deserves. [ABSTRACT FROM AUTHOR]

Ionic liquids (ILs) are being designed as green alternatives to volatile organic solvents that are currently used in a wide range of industrial processes. While knowledge about the toxicity of various ILs to aquatic organisms has expanded in recent years, toxicity data remains limited to a few animal taxa. Furthermore, few studies have examined the non-lethal effects of ILs on aquatic organisms. We investigated how ILs affect mortality and feeding of the zebra mussel (Dreissena polymorpha), a sessile bivalve that is invasive in North America and much of Europe. Lethal effects of six ILs (butyl-, hexyl-, or octyl-chains on either a methylpyridinium or methylimidazolium cationic base) were studied with 96 h acute bioassays. Filtering behavior was examined in 2 h feeding trials of individual mussels fed Chlamydomonas reinhardtiiand exposed to sublethal concentrations of the three imidazolium-based ILs. The ILs tested caused acute mortality over a wide range of concentrations (LC50= 21.4 to 1290 mg L−1), ILs with longer alkyl chains were more toxic, and pyridinium- and imidazolium-based ILs had similar toxicities. When comparing toxicity to the same IL across organisms, zebra mussels exhibit some of the highest LC50values and would be among the most resistant aquatic organisms in the event of an IL release. Short-term exposure to any IL reduced zebra mussel feeding. For butyl- and hexyl-chain ILs, feeding was significantly reduced at the acute LC50, whereas the octyl-chain IL reduced feeding at the acute LC5. Reduced survival and feeding by zebra mussels in the presence of ILs could have substantial effects on other trophic levels, which could lead to changes to affected ecosystems that would not have been predicted solely from standard mortality bioassays. Based on our mortality data, ILs are no worse than currently used industrial solvents; however, results from our feeding experiments demonstrate the potential for significant effects at sublethal concentrations. Consequently, we suggest that more ecologically relevant endpoints should be incorporated into a thorough hazard assessment of ILs. [ABSTRACT FROM AUTHOR]

According to Directive 2008/50/EC, all equipment used for NO(x) fixed measurements must comply with the reference method or equivalent by 11 June 2013. Up to that date, non-type-approved equipment can continue to be used in air monitoring networks and will probably also be used in air pollution studies even after that date. In this context, it is advisable to know how these "old" measuring instruments respond to the requirements of European Standard EN 14211: 2005: Ambient Air Quality--Standard method for the measurement of the concentration of nitrogen dioxide and nitrogen monoxide by chemiluminescence. In this work, we carry out a thorough performance evaluation of two commercial NO(x) analysers, both purchased before 2010, according to the mentioned Standard. Results show that water vapour interference is one of the most important disconformities with this Standard. The expanded uncertainty estimation of both analysers was below the quality objective of the above-mentioned Directive (15%); however, this calculation was performed by applying some modifications to the proposed model of Standard EN 14211. These modifications are also discussed in the paper.

This paper describes how sixteen partners from eight different countries across Europe are working together in two EU projects focused on the development of a point of care system. This system uses disposable Lab on a Chips (LOCs) that carry out the complete assay from sample preparation to result interpretation of raw samples. The LOC is either embedded in a flexible motherboard with the form of a smartcard (Labcard) or in a Skinpatch. The first project, OPTOLABCARD, extended and tested the use of a thick photoresit (SU-8) as a structural material to manufacture LOCs by lamination. This project produced several examples where SU-8 microfluidic circuitry revealed itself as a viable material for several applications, such as the integration on chip of a Polymerase Chain Reaction (PCR) that includes sample concentration, PCR amplification and optical detection of Salmonella spp. using clinical samples. The ongoing project, LABONFOIL, is using two results of OPTOLABCARD: the sample concentration method and the capability to fabricate flexible and ultra thin LOCs based on sheets instead of wafers. This rupture from the limited and expensive wafer surface heritage allows the development of a platform where LOCs are big enough to include all the sample preparation subcomponents at a low price. These LOCs will be used in four point of care applications: environment, food, cancer and drug monitoring. The user will obtain the results of the tests by connecting the Labcard/Skinpatch reader to a very popular interface (a smartphone), creating a new instrument namely "The SmartBioPhone". All standard smartphone capabilities will be at the disposal of the point of care instrument by a simple click. In order to guarantee the future mass production of these LOCs, the project will develop a large dry film equipment where LOCs will be fabricated at a low cost.

The application of diffuse pollution models included in EUROHARP encompassed varying levels of parameterisation and approaches to the preparation of input data depending on the model and modelling team involved. Modellers consistently faced important decisions in relation to data interpretation, especially in those catchments with unfamiliar physical or climatic characteristics, where catchment conditions were beyond the range for which a particular model was originally developed, or where only limited input data were available. In addition to a broad discussion of data issues, this paper compares the performance of the four sub-annual output models tested in EUROHARP (EveNFlow, NL-CAT, SWAT and TRK) in three test catchments without the modelling teams having sight of measured flow and nitrate concentration data. Model performance in this "blind test" indicate that the range of predictions generated by any individual models pre and post calibration exceed the differences between the estimates yielded by all four models. Comparison of Analysis of Variance (ANOVA) statistics for simulated and observed flow, concentration and loads underscores the benefits of calibration for these intermediate and complex model formulations. Interpretation of input data (e.g. rainfall interpolation method and pedotransfer functions selected) appeared equally (or more) important than process representation. In the absence of calibration data, modeller unfamiliarity with a particular catchment and its environmental processes sometimes resulted in questionable assumptions and input errors which highlight the problems facing modellers charged with implementing policies under the Water Framework Directive (2000/60/EC) in poorly monitored catchments. Catchment data owners and modellers must therefore work more closely given that the output from diffuse pollution models is clearly modeller-limited as well as model-limited.

The EC-funded EUROHARP project studies the harmonisation of modelling tools to quantify nutrient losses from diffuse sources. This paper describes a set of study areas used in the project from geographical conditions, to land use and land management, geological and hydro-geological perspectives. The status of data availability throughout Europe in relation to the modelling requirements is presented. The relationships between the catchment characteristics and the nutrient export are investigated, using simple data available for all the catchments. In addition, this study also analyses the hydrological representativity of the time series utilised in the EUROHARP project.

The need for action to avoid long term deterioration of fresh water quantity and quality was recognised by the Ministerial Seminar on groundwater held at The Hague in November 1991, which underlined the need to establish a programme of actions to be implemented by the year 2000 at national and Community level, aiming at sustainable management and protection of water resources. These discussions resulted in a communication from the European Commission in 1996 about a "Groundwater Action Programme" (GWAP). In parallel, a wide consultation had started on the need to streamline EU water policies, which led to the adoption of the Water Framework Directive in December 2000, later complemented by a "daughter" groundwater directive adopted in December 2006. This paper recalls the orientations of the GWAP and studies whether and how recommended actions have been tackled by the actual EU regulatory framework.

A wide range of environmental policies are based on the monitoring of chemical and/or biological parameters which are used to evaluate the environmental status of relevant compartments (e.g. water, soil, air) with the ultimate aim of making appropriate management decisions. The soundness of policy decisions is therefore directly related to the reliability of the environmental monitoring programmes. Monitoring reliability in turn is predominantly linked to scientific and technological progress. Hence a correct design, development and implementation process of environmental policies is, at least in part, dependent upon a proper integration of scientific and technological advances (in monitoring, but also for all kinds of permit procedures, remediation strategies etc.). This paper examines science-policy integration needs in support of groundwater environmental monitoring, with focus on on-going policy developments. The article aims to summarise key information on groundwater policy and EU scientific developments to raise awareness of the scientific community involved in this issue and to enhance communication among scientists and policy-makers.

Amongst the mass of academic papers and policy reports it is easy to forget that scientists and regulators are not the only ones concerned about the environment. The public has an interest too. Aided by modern technology and increasing legal rights, the man in the street has access to environmental information as never before. In this article we review recent developments in public assess and what these mean for 'the experts'.

In order to control the quality of rare earth determinations in environmental matrices, the Standards, Measurements and Testing Programme (formerly Community Bureau of Reference, BCR) of the European Commission has started a project, the final aim of which is to certify four types of matrices (tuna muscle, mussel tissue, aquatic plant and estuarine sediment) for their contents of a range of rare earth elements (Sc, Y and the lanthanides: La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb and Lu). The elements U and Th were added to the study. The first part of this project consisted of an interlaboratory study which aimed to test the feasibility of preparation of environmental reference materials and to detect and remove most of the pitfalls observed in rare earth determinations. This paper presents the preparation of the four matrices for the intercomparison study and for the candidate reference material. The main results are presented of the interlaboratory study that was carried out prior to the certification campaign. This collaborative trial is the first attempt ever carried out at this scale to evaluate the state-of-the-art of rare earth determinations in the environment. Its impact on the improvement of chemical measurements will have positive effects on the comparability of data necessary for environmental monitoring.