11 results on '"Brodin, Ellen"'
Search Results
2. Postprandial lipemia is not increased in patients with previous unprovoked venous thromboembolism.
- Author
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Hald, Erin M., Brækkan, Sigrid K., Vik, Anders, Brodin, Ellen E., and Hansen, John-Bjarne
- Subjects
LIPEMIA ,THROMBOEMBOLISM ,CARDIOVASCULAR diseases risk factors ,ARTERIAL diseases ,BLOOD lipid measurement ,BLOOD lipoproteins ,PATIENTS - Abstract
Background: Patients with arterial cardiovascular disease have increased postprandial lipemia, and plasma levels of postprandial remnants are related to the progression of atherosclerosis. Studies have shown that patients with unprovoked venous thromboembolism have increased risk of arterial cardiovascular disease. Objective: To investigate whether patients with a history of unprovoked venous thromboembolism have increased postprandial lipemia. Methods: A population-based case−control study was performed in 20 patients with a history of unprovoked venous thromboembolism and 20 age- and gender-matched healthy controls. Participants were subjected to a standard fat tolerance test (1 g/kilo body weight) with subsequent blood sampling every second hour for 8 hours. Lipids were measured by traditional methods and lipoprotein subclasses by proton nuclear magnetic resonance. Results: Fasting lipids and lipoprotein subclasses did not differ between groups. The postprandial lipemia, assessed by the incremental area under the triglyceride curve, was not different in venous thromboembolism patients and healthy controls (5.0 ± 3.6 mmol/L∗h vs 5.3 ± 4.4 mmol/L∗h, P = .81). Similarly, the distribution and size of the lipoprotein subclasses obtained 4 hours postprandially did not differ between groups. Conclusion: Patients with a history of unprovoked venous thromboembolism had similar lipoprotein subclasses size, distribution, and postprandial lipemia as healthy controls. Our findings indicate that postprandial lipemia is not a link between unprovoked venous thromboembolism and arterial cardiovascular disease. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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3. Regulation of thrombin generation by TFPI in plasma without and with heparin.
- Author
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Brodin, Ellen, Appelbom, Hege, Østerud, Bjarne, Hilden, Ida, Petersen, Lars C., and Hansen, John-Bjarne
- Abstract
The purpose of this study was to investigate the impact of recombinant glycosylated TFPI (rg-TFPI) from BHK cells, nonglycosylated TFPI (r-TFPI) from Escherichia coli, and truncated TFPI
1-161 on thrombin generation (TG) in plasma treated with and without heparin in vitro and ex vivo. Fasting plasma samples were collected from 6 healthy persons. TG was assessed by the calibrated automated thrombography (CAT) method. The addition of increasing concentrations (0–200 ng/mL) of different TFPI caused a 5% to 30% prolongation of lag time for TF (3.0 pM) induced TG, with the most pronounced effect for rg-TFPI and the least pronounced effect for truncated TFPI, but without affecting endogenous thrombin potential (ETP) in TF-induced coagulation. Removal of native TFPI from plasma by anti-TFPI IgG treatment shortened lag time by 35 ± 4% without affecting ETP. Increasing concentrations (0–200 ng/mL) of various TFPI in the presence of low heparin concentrations (0.1 IU/mL) prolonged lag time and decreased ETP by 25% to 75% with the most prominent effect promoted by glycosylated full-length TFPI. The effect of neutralizing antibodies against TFPI and antithrombin (AT) was studied in plasma in the presence of heparin administered in vitro or ex vivo. The results revealed that TFPI and AT acted in synergy as inhibitors of coagulation in terms of the effect on both initiation (lag time) and propagation (ETP). Our data demonstrated that the CAT assay appropriately assessed the impact of TFPI on initiation and propagation of TG in a physiological plasma milieu with and without heparin. TFPI contributed significantly to regulation of coagulation initiation (lag time). The C-terminal region and, to a lesser extent, glycosylation of the TFPI molecule were essential for its anticoagulant function in the absence and presence of heparin. [Copyright &y& Elsevier]- Published
- 2009
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4. Impact of native VLDL on tissue factor pathway inhibitor in endothelial cells and interactions between TFPI and lipoprotein lipase.
- Author
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Brodin, Ellen, Iversen, Nina, and Hansen, John-Bjarne
- Abstract
Tissue factor pathway inhibitor (TFPI) is a potent inhibitor of tissue factor (TF)-induced blood coagulation. A positive association between very low density lipoproteins (VLDLs) and TFPI has been reported in vivo. In contrast, one in vitro study indicates that TFPI may enhance lipoprotein lipase (LPL) activity, thereby increasing triglyceride hydrolysis. The current study was conducted to investigate how native VLDL influenced the synthesis and release of TFPI in endothelial cells, and how TFPI affected the LPL-induced hydrolysis of VLDL in vitro and at the endothelial surface. A spontaneously transformed immortal endothelial cell line (ECV304) and primary coronary artery cells (CoEc) were used, and VLDL was isolated from healthy volunteers by density gradient ultracentrifugation. Sequential free fatty acid (FFA) measurements were used to evaluate the kinetics of the LPL-induced hydrolysis. The levels of TFPI mRNAs in the stimulated cells were determined by quantitative real-time reverse transcription-ploymerase chain reaction (qPCR) using the ABI PRISM 7700 Sequence Detection System. Stimulation of ECV304 cells for 24 hours with native VLDL (0–100 ?g/mL) caused a dose-dependent increase of TFPI in the medium (6.7–23.8 ng/10
6 cells, P < 0.001), without affecting the cellular content of TFPI. The expression of TFPI mRNA was significantly upregulated after 10 minutes of stimulation with n-VLDL. Both recombinant TFPI (r-TFPI) and LPL showed a dose-dependent binding to ECV 304 cells without saturation, and no competitive binding interactions between LPL and TFPI were observed at the endothelial surface. The addition of increasing concentrations of r-TFPI to ECV 304 cells, preincubated with LPL, did not affect the hydrolysis of VLDL triglycerides. The maximal reaction velocity (Vmax ) of LPL-induced hydrolysis of n-VLDL was not affected by the addition of increasing concentrations of r-TFPI to the reaction mixture in vitro. The current experimental study indicates an upregulation of TFPI synthesis and release by VLDL. LPL-induced hydrolysis of VLDL in vitro was not influenced by TFPI neither in suspension nor at the endothelial surface. [Copyright &y& Elsevier]- Published
- 2006
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5. C0110 Serum levels of vitamin D is not associated with future risk of venous thromboembolism–the Tromsø Study
- Author
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Brodin, Ellen, Lerstad, Gunhild, Grimnes, Guri, Brækkan, Sigrid K., Svartberg, Johan, Jorde, Rolf, and Hansen, John-Bjarne
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- 2012
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6. Serum osteoprotegerin and future risk of venous thromboembolism. The Tromsø study
- Author
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Vik, Anders, Brodin, Ellen, Brækkan, Sigrid K., and Hansen, John-Bjarne
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- 2012
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7. Tissue factor-induced Thrombin Generation in the Fasting and Postprandial State among Elderly Survivors of Myocardial Infarction
- Author
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Lekhal, Samira, Børvik, Trond, Brodin, Ellen, Nordøy, Arne, and Hansen, John-Bjarne
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THROMBOPLASTIN , *MYOCARDIAL infarction , *THROMBIN , *BLOOD coagulation , *PROTHROMBIN , *TRIGLYCERIDES , *LIPEMIA , *MEDICAL statistics - Abstract
Abstract: Introduction: Tissue factor (TF)-induced thrombin generation (TG) ex vivo has been suggested to be an important method to assess thrombotic risk. No studies have investigated the impact of postprandial lipemia on TF-induced TG. Since myocardial infarction (MI) is associated with elevated postprandial levels of triglycerides, we hypothesized a differential impact of postprandial lipemia on coagulation activation in MI-patients and healthy controls. Material and Methods: Elderly survivors of acute MI (n=44) and healthy age-and sex matched controls (n=43) underwent a fat tolerance test (1 gram per kg body weight) to assess coagulation activation during postprandial lipemia. Results: The incremental area under the curve (AUCi) for serum triglycerides was higher in MI-patients than in healthy age-and sex matched controls (5.64±0.52mmol/L⁎h and 3.94±0.39mmol/L⁎h, p=0.012) during the postprandial phase. Subsequent endogenous activation of coagulation, assessed by FVIIa and thrombin generation (F1+2), was similar among groups and not related to levels of triglycerides during the postprandial phase. Healthy individuals had a gradual decline in TF-induced thrombin generation ex vivo, assessed by endogenous thrombin potential (ETP) (AUCi=-542.4±71.4nM⁎min⁎h, p<0.001), whereas MI-patients retained their ETP (AUCi=127.4±89.0nM⁎min⁎h, p=0.47) in plasma during the postprandial phase (p for group difference=0.005). Conclusions: MI-patients had elevated postprandial lipemia and retained their ability for TF-induced TG in plasma ex vivo in the postprandial phase, whereas the capacity gradually decreased in healthy individuals. Further studies are warranted to reveal underlying mechanism(s) and clinical implications. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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8. Elevated levels of platelet microparticles in carotid atherosclerosis and during the postprandial state
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Michelsen, Annika E., Notø, Ann–Trude, Brodin, Ellen, Mathiesen, Ellisiv B., Brosstad, Frank, and Hansen, John–Bjarne
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BLOOD platelets , *PARTICLES , *ATHEROSCLEROSIS , *CAROTID artery , *THROMBOSIS complications , *HYPERTRIGLYCERIDEMIA , *CORONARY heart disease risk factors - Abstract
Abstract: Background: Platelet microparticles (PMPs) possess proatherogenic and procoagulant properties which may play a role in atherogenesis and subsequent thromboembolic complications. The present study was conducted to investigate the possible relationship between carotid atherosclerosis and plasma concentrations of PMPs, and elucidate if plasma levels of PMPs were affected by postprandial hypertriglyceridemia. Methods and results: Subjects with ultrasound-assessed carotid atherosclerotic plaques (echogenic; n=20 and echolucent; n=20), assessed by ultrasonography, and subjects without carotid plaques (n=20) were recruited from a population-based study and underwent a standard fat tolerance test. Subjects with carotid plaques had significantly higher levels of large PMPs than subjects without carotid atherosclerotic plaques (96.7±50.4 µg/l versus 56.1±34.9 µg/l), after adjustments for traditional cardiovascular risk factors and use cardiovascular drugs (p=0.021). Plasma PMPs were not associated with plaque echogenicity. Postprandial hypertriglyceridemia induced a similar increase in plasma PMPs within all groups. Significant correlations were found between an increase in plasma triglycerides and percent elevation in total PMPs (r=0.29, p<0.05) and large PMPs (r=0.34, p<0.01) in the postprandial phase. Conclusions: Individuals with echogenic and echolucent carotid atherosclerotic plaques have statistically significant elevation of large plasma PMPs compared to age/sex-matched normal controls. Postprandial hypertriglyceridemia induces a significant, similar increase in plasma PMPs in individuals with and without carotid atherosclerotic plaques which could be of pathophysiological importance in atherogenesis. [Copyright &y& Elsevier]
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- 2009
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9. Markers of endothelial cell activation and neutrophil extracellular traps are elevated in immune thrombocytopenia but are not enhanced by thrombopoietin receptor agonists.
- Author
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Garabet, Lamya, Henriksson, Carola E., Lozano, María Luisa, Ghanima, Waleed, Bussel, James, Brodin, Ellen, Fernández-Pérez, María Piedad, Martínez, Constantino, González-Conejero, Rocío, Mowinckel, Marie-Christine, and Sandset, Per Morten
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THROMBOPOIETIN receptor agonists , *ENDOTHELIAL cells , *IDIOPATHIC thrombocytopenic purpura , *CELL adhesion , *THROMBOMODULIN - Abstract
Patients with immune thrombocytopenia (ITP) are at increased risk of thrombosis, which seems to be further enhanced by treatment with thrombopoietin-receptor-agonists (TPO-RAs). The underlying mechanisms of thrombosis in ITP are not fully understood. Endothelial cell activation and neutrophil extracellular traps (NETs) play important roles in thrombosis, however, their roles in ITP itself, or in TPO-RA-treatment, have not yet been fully explored. We aimed to investigate whether endothelial cell activation and NETs are involved in the hypercoagulable state of ITP, and whether TPO-RA-treatment enhances endothelial cell activation and NET formation. We measured markers of endothelial cell activation including intercellular adhesion molecule-1 (ICAM-1), vascular adhesion molecule-1 (VCAM-1) and thrombomodulin in 21 ITP patients, and E-selectin in 18 ITP patients. Markers of NET formation, citrullinated histone H3-DNA (H3Cit-DNA) and cell-free DNA (cfDNA), were measured in 15 ITP patients. All markers were measured before, and 2 and 6 weeks after initiation of TPO-RA-treatment in ITP patients, and in matched controls. Higher levels of ICAM-1, thrombomodulin, and H3Cit-DNA were found in ITP patients, both before and after TPO-RA-treatment, compared with controls. No differences were found for VCAM-1, E-selectin or cfDNA. TPO-RA-treatment did not further increase markers of endothelial cell activation or NET formation. This study showed that ITP patients have increased endothelial cell activation and NET formation, both of which may contribute to the intrinsic hypercoagulable state of ITP. TPO-RA-treatment, however, did not further increase endothelial cell activation or NET formation indicating that other drug-associated prothrombotic mechanisms are involved. • ITP patients have higher ICAM-1 and thrombomodulin than controls suggesting increased endothelial cell activation/injury. • ITP patients have higher NET marker H3Cit-DNA than controls indicating increased NET formation in these patients. • Treatment with TPO-RAs does not enhance endothelial cell activation or NET formation. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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10. Inter-rater agreement between professional-rated and patient-rated scores of the Villalta scale for evaluation of the post-thrombotic syndrome.
- Author
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Isaksen, Trond, Tichelaar, Y.I.G. Vladimir, Skjeldestad, Finn E., Brodin, Ellen E., Vik, Anders, Singh, Kulbir, and Hansen, John-Bjarne
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POSTTHROMBOTIC syndrome , *VENOUS thrombosis , *STANDARD deviations , *CONFIDENCE intervals , *THROMBOSIS - Published
- 2016
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11. Oral Anticoagulation Therapy for Venous Thromboembolism in Norway: Time Trends and Treatment Patterns.
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Ghanima, Waleed, Schultze, Anna, Donaldson, Robert, Brodin, Ellen, Halvorsen, Sigrun, Graham, Sophie, Carroll, Robert, Ulvestad, Maria, and Lambrelli, Dimitra
- Published
- 2021
- Full Text
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