1. Swine acute diarrhea syndrome coronavirus replication is reduced by inhibition of the extracellular signal-regulated kinase (ERK) signaling pathway.
- Author
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Zhang, Jiyu, Zhang, Liaoyuan, Shi, Hongyan, Feng, Shufeng, Feng, Tingshuai, Chen, Jianfei, Zhang, Xin, Han, Yuru, Liu, Jianbo, Wang, Yiming, Ji, Zhaoyang, Jing, Zhaoyang, Liu, Dakai, Shi, Da, and Feng, Li
- Subjects
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CELLULAR signal transduction , *VIRAL transmission , *SWINE , *DIARRHEA , *VIRUS diseases , *MIDDLE East respiratory syndrome , *COVID-19 - Abstract
Swine acute diarrhea syndrome coronavirus (SADS-CoV) is a newly discovered enteric coronavirus. We have previously shown that the caspase-dependent FASL-mediated and mitochondrion-mediated apoptotic pathways play a central role in SADS-CoV-induced apoptosis, which facilitates viral replication. However, the roles of intracellular signaling pathways in SADS-CoV-mediated cell apoptosis and the relative advantages that such pathways confer on the host or virus remain largely unknown. In this study, we show that SADS-CoV induces the activation of ERK during infection, irrespective of viral biosynthesis. The knockdown or chemical inhibition of ERK1/2 significantly suppressed viral protein expression and viral progeny production. The inhibition of ERK activation also circumvented SADS-CoV-induced apoptosis. Taken together, these data suggest that ERK activation is important for SADS-CoV replication, and contributes to the virus-mediated changes in host cells. Our findings demonstrate the takeover of a particular host signaling mechanism by SADS-CoV and identify a potential approach to inhibiting viral spread. • SADS-CoV activated the ERK signaling pathway irrespective of viral replication. • Chemical inhibition and ERK1/2 knockdown markedly impaired SADS-CoV replication. • There were crosstalk between ERK and apoptotic pathways during SADS-CoV infection. • SADS-CoV exploits the ERK cascade to complete successful viral infection. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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