1. Mulberry anthocyanins exert anti-AGEs effects by selectively trapping glyoxal and structural-dependently blocking the lysyl residues of β-lactoglobulins.
- Author
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Khalifa, Ibrahim, Xia, Du, Dutta, Kunal, Peng, Jinmeng, Jia, Yangyang, and Li, Chunmei
- Subjects
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ANTHOCYANINS , *GLYOXAL , *ADVANCED glycation end-products , *LACTOGLOBULINS , *MULBERRY - Abstract
• Mulberry anthocyanins shielded β-lactoglobulin against glycation through different mechanisms. • Mulberry anthocyanins dose-dependently mitigated creation of advanced glycation end-products. • Mulberry anthocyanins trapped glyoxal more than methylglyoxal and formed some glyoxal adducts. • Mulberry anthocyanins declined protein-glucose binding and structurally blocked lysyl sites. • Mulberry anthocyanins could be beneficially valorised as functional antiglycation ingredients. Advanced glycation end-products (AGEs), which instigate many disorders, are mostly mediated by dicarbonyl rearrangements. We studied the corresponding mechanisms of the anti-glycation effects of two anthocyanins purified from mulberry fruits, namely cyanidin 3-glucoside (C3G) and cyanidin 3-rutinoside (C3R), on glycated β-lactoglobulins (β-Lg). Both mulberry anthocyanins (MAs) inhibited the AGEs-formation in a dose-dependent manner, but the effect of C3R was significantly stronger than that of C3G (p < 0.05). MAs inhibited AGEs-formation by selectively trapping dicarbonyls, especially glyoxal. The UPLC-ESI-Q-TOF-MS results characterized that C3R formed mono- and di-glyoxal adducts, where C3G only created di-glyoxal adducts. Additionally, C3R could directly interact with some of the glycation sites of β-Lg. Overall, GO-trapping and β-Lg-MAs covalent/noncovalent binding are disclosed as the key mechanisms of the anti-AGEs activity of MAs on β-Lg, which could be valorised as effectual AGEs inhibitors in proteins-rich matrices. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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