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This study aimed to implement a targeted multiple reaction monitoring (MRM) screening strategy using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) for the initial detection of 165 compounds of emerging concern (CECs) in water, sediment, and fish samples. Following the screening, confirmatory and quantitative analyses were conducted using analytical standards for the detected compounds. Qualitative results were confirmed using high-resolution mass spectrometry (HRMS) for those CECs without available standards. Ecotoxicological and human health risk assessments were performed for the quantified CECs. The analysis identified 35 suspect CECs (12 quantified with analytical standards), including parent compounds and metabolites of anti-inflammatories, antibiotics, antidepressants, sedatives, stimulants, and illicit drugs. High concentrations of these CECs were particularly evident near a Wastewater Treatment Plant (WWTP), where notable levels of compounds such as caffeine (4.02-15.03 ng L -1 ), ciprofloxacin (6.05 ng L -1 ), clindamycin (6.04-7.01 ng L -1 ), and diclofenac (1.36-2.20 ng L -1 ) were detected. Sediment samples exhibited the highest incidence of CECs, with caffeine reaching the highest concentration (55.89 μg kg -1 ). Ciprofloxacin (2.94 to 4.18 μg kg -1 ) was the sole CEC detected in biota samples. The ecotoxicological risk assessment indicated that the concentrations of all detected compounds posed significant ecotoxicity risks to the aquatic environment. In particular, caffeine and diclofenac presented considerable acute and chronic toxic risks to aquatic organisms, including algae, crustaceans, and fish. The Hazard Index (HI) values (3.65 -7 to 8.06 -8 ) suggest that ingesting ciprofloxacin at the concentrations found in fish does not represent a significant risk to human health. However, due to the reported risks to estuarine biota, it is crucial to continuously monitor the accumulation of these compounds in food widely consumed by the local population to assess potential impacts on human health., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Silvani Verruck reports financial support was provided by Foundation for Research Support and Innovation of Santa Catarina State (FAPESC) and National Council for Scientific and Technological Development (CNPq). The other authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

Polycyclic aromatic hydrocarbons (PAHs) are carcinogenic organic pollutants found in various environments, notably aquatic ecosystems and the food chain, posing significant health risks. Traditional methods for detecting PAHs in food involve complex processes and considerable reagent usage, raising environmental concerns. This study explores eco-friendly approaches suing solid phases derived from natural sources in matrix solid phase dispersion. We aimed to develop, optimize, and validate a sample preparation technique for seafood, employing natural materials for PAH analysis. Ten natural phases were compared with a commercial reference phase. The methodology involved matrix solid phase dispersion and pressurized liquid extraction, followed by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Three solid phases (perlite, sweet manioc starch, and barley) showed superior performance in LC-MS/MS and were further evaluated with gas chromatography-tandem mass spectrometry (GC-MS/MS), confirming perlite as the most effective phase. Validation followed Brazilian regulatory guidelines and European Community Regulation 2021/808/EC. The resulting method offered advantages in cost-effectiveness, reduced environmental impact, cleaner extracts, and enhanced analytical performance compared to the reference solid phase and LC-MS/MS. Proficiency analysis confirmed method reliability, with over 50% alignment with green analytical chemistry principles. In conclusion, this study developed an environmentally sustainable sample preparation technique for seafood analysis using natural solid phases, particularly perlite, for PAH determination., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Objective: Evaluate the association between oral health-related quality of life (OHRQoL) and self-reported symptoms of TMD., Methods: Representative samples of older adults (≥60 years) were included ( n  = 569). Both TMD symptoms and OHRQoL were assessed by Fonseca Anamnestic Index (FAI) and Oral Health Impact Profile-14 (OHIP-14), respectively. Prevalence (those answering "frequently" or "always" in at least one question), severity (total means scores), and extent (number of questions answered as "frequently" or "always") of OHRQoL were estimated., Results: Overall, 33.4% and 9.5% had mild or moderate/severe TMD symptoms. Those with any symptom of TMD had a prevalence ratio (PR) 38% higher for the worst OHRQoL (95% confidence interval [95%CI]:1.04-1.82) compared to those without TMD symptoms. Worst OHRQoL were observed for those with mild (PR:1.35; 95%CI:1.01-1.81) and moderate/severe TMD (PR:1.53; 95%CI:1.04-2.26). Similar results were detected in the severity and extent of OHRQoL., Conclusion: Severity TMD was associated with worse ORHQoL.

One of the impacts related to mining dam failures is the change in water quality downstream of the rupture and a knowledge gap is observed in terms of methodologies aimed at the prognosis of impact in water abstractions, a vulnerability that can be identified before a rupture event. Thus, the present work aims to describe a novel methodological proposal, not currently considered by control agencies, of a standard protocol that enables a comprehensive prognosis of the impacts on water quality impact in scenarios of dam failure. Initially, extensive bibliographic research was carried out on the main disruptions events since 1965 intended to better comprehend the impacts on water quality and to identify mitigatory actions proposed by the time. The information provided a framework to propose a conceptual model for the prognosis of water abstractions, with the suggestion of software and studies to comprehend the different scenarios in the event of dam failure. A protocol was prepared to obtain information on potentially affected inhabitants and a multicriteria analysis was developed using the Geographic Information System (GIS) to suggest the employment of preventive and corrective actions. The methodology was demonstrated in the Velhas River basin considering a hypothetical scenario of tailing dam failure. Changes in water quality would be observed in 274 km of its extension, mainly related to alterations in solids, metals, and metalloids' concentration, in addition to the impact on important water treatment plants. The map algebra and the results suggest the need for structuring actions in cases where the water abstraction is intended for human supply and in populations greater than 100,000 inhabitants. Populations smaller than these, or usages other than human supply, could be supplied by water tank trucks or mixed alternatives. The methodology pointed out the necessity for structuring supply actions with due notice, with the potential to prevent water shortages in scenarios of tailing dam failure and to complement the enterprise resource planning of mining companies., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

Objective: This study aimed to evaluate the prevalence and factors associated with self-perception of teeth alignment and colour in adolescents., Methods: This cross-sectional study was carried out in public and private schools in Passo Fundo, Brazil. A representative sample of students aged 15 to 19 years were included. The outcome of this study was self-perception of teeth alignment and colour according to selected items from a structured and validated questionnaire (the Child's and Parent's Questionnaire about Teeth Appearance). Bivariate and multivariable analysis, using Poisson regression with robust variance, were used. A P value <.05 was established for statistical significance., Results: The study included 736 adolescents. The worst self-perception of teeth alignment and colour was observed in 46.5% (n = 342) of the adolescents. Nonsmokers presented a prevalence ratio (PR) 29% lower (95% CI, 0.57-0.89) for the worst self-perception of teeth alignment and colour when compared to smokers/former smokers. Adolescents with highly educated mothers presented a lower prevalence of negative self-perception than those with mothers with a low level of education (PR: 0.66; 95% CI, 0.51-0.86). Presence of orthodontic treatment with (PR: 0.76; 95% CI, 0.62-0.94) or without (PR: 0.61; 95% CI, 0.46-0.76) tooth whitening were associated with better self-perception of teeth alignment and colour., Conclusions: Exposure to smoking and lower maternal level of education were associated with worse self-perception of teeth alignment and colour in adolescents. A history of orthodontic treatment with or without tooth whitening was associated with a lower concern with aesthetics., Competing Interests: Conflict of interest The authors report no conflict of interest related to this study., (Copyright © 2021. Published by Elsevier Inc.)