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Abstract This study presents a dual-functional thin film, known as Ag nanoparticles decorated, ZnO nanorods coated silica nanofibers (AgNP-ZnONR-SNF), which demonstrates remarkable capabilities in both water purification and organic pollutants sensing. The 3D fibrous structure of ZnONR-SNF provides a large surface-area-to-volume ratio for piezo- and photo-catalytic degradation of organic pollutants under UV irradiation, achieving over 98% efficiency. Ag nanoparticles decorated on ZnONR-SNF form “hot-spot” that significantly enhance the surface-enhanced Raman spectroscopy (SERS) signal, resulting in an enhancement factor of 1056 and an experimental detection limit of 1 pg mL−1. Furthermore, a machine learning algorithm is developed for the qualitative and quantitative detection of multiple contaminants, achieving high accuracy (92.3%) and specificity (89.3%) without the need for preliminary processing of Raman spectra. This work provides a promising nanoengineering solution for water purification and sensing with improved detection accuracy, purification efficiency, and cost-effectiveness.

This study aims to investigate the effect of different brining conditions on the aroma of the marinade, gas chromatography-mass spectrometry was used to determine the volatile composition of the marinade samples with different brining times in atmospheric pressure (0525-1 and 0525-4) and micro-pressure (0525-H), combining with sensory ranking test and Principal Component Analysis was used to further analyze the differences in the aroma of the three marinades. The results showed that a total of 43 volatile compounds were detected by GC-MS, including alcohols, phenols, ketones, alkenes, esters, ethers, acids and other compounds. More volatile compounds were founded in the marinade solutions under atmospheric pressure than under micro-pressure conditions, but the total content of marinade solutions under atmospheric pressure was lower than the under micro-pressure conditions. For the OAV, a total of 15 substances that contributed to the aroma of the marinade, of which the aroma of the marinade under atmospheric conditions were richness, while the aroma of the marinade under micro-pressure conditions was stronger. The results of ranking the aroma intensity of the samples were 0525-H>0525-1>0525-4 with significant difference (P

To attain the differences in the flavor profile of Douchi, the key aroma-active compounds of three types of Douchi were investigated. The “Sauce-like”, “Smoky”, “Nutty”, “Roast”, “Caramel”, and “Flower” of Douchi were favored by customers. Further, a total of 179 volatile compounds were identified using HS-SPME-GC-MS, and 29 aroma compounds were detected using GC-O-MS. Based on the quantification, 9, 13, and 10 compounds were regarded as aroma-active compounds in Yangjiang Douchi (YJ), Pingjiang Douchi (PJ), and Liuyang Douchi (LY), respectively. Moreover, the mixture of these aroma-active compounds successfully simulated the main aromas of PJ, LY, and YJ. And omission experiments confirmed that guaiacol was the key aroma compound for LY, benzene acetaldehyde, dimethyl trisulfide, and 2-acetyl pyrrole were important for YJ, benzene acetaldehyde and 3,5-diethyl-2-methyl pyrazine notably contributed to key aroma of PJ.

Starting from February 2023, the International Laser Ranging Service (ILRS) began releasing satellite laser ranging (SLR) data for all BeiDou global navigation satellite system (BDS-3) medium earth orbit (MEO) satellites. SLR data serve as the best external reference for validating satellite orbits, providing a basis for comprehensive evaluation of the BDS-3 satellite orbit. We utilized the SLR data from February to May 2023 to comprehensively evaluate the orbits of BDS-3 MEO satellites from different analysis centers (ACs). The results show that, whether during the eclipse season or the yaw maneuver season, the accuracy was not significantly decreased in the BDS-3 MEO orbit products released from the Center for Orbit Determination in Europe (CODE), Wuhan University (WHU), and the Deutsches GeoForschungsZentrum (GFZ) ACs, and the STD (Standard Deviation) of SLR residuals of those three ACs are all less than 5 cm. Among these, CODE had the smallest SLR residuals, with 9% and 12% improvement over WHU and GFZ, respectively. Moreover, the WHU precise orbits exhibit the smallest systematic biases, whether during non-eclipse seasons, eclipse seasons, or satellite yaw maneuver seasons. Additionally, we found some BDS-3 satellites (C32, C33, C34, C35, C45, and C46) exhibit orbit errors related to the Sun elongation angle, which indicates that continued effort for the refinement of the non-conservative force model further to improve the orbit accuracy of BDS-3 MEO satellites are in need.

Background: Neurofibroma type I (NF1) often presents with multiple clinical phenotypes due to mutations of NF1 gene. The aim of this study was to determine the phenotypic and therapeutic relevance of tumor microenvironment in NF1 patients. Methods: Tumor stem cells (TSCs) from NF1 were isolated and cultured using fluorescence activated cell sorting (FACS) and colony formation experiments. Then, flow cytometry was used to detect the surface markers, osteogenic and adipogenic differentiation were performed as well. Its tumorigenesis ability was confirmed by subcutaneous tumorigenesis in nude mice. Immunohistochemical staining was performed on neurofibroma tissues from the head and trunk with different phenotypes. The expression of BDNF in neurofibroma tissues was detected by Elisa and immunohistochemical staining. Western Blotting was used to detect the expression of p38 MAPK pathway in TSCs. The effect of BDNF neutralizing antibody on the tumorigenesis of TSCs was observed. Results: Herein, we advocate that NF1 contain a new subgroup of mesenchymal-like neurofibroma stem cells (MNSCs). Such colony-forming MNSCs preserved self-renewal, multiple differentiation and tumorigenic capabilities. More interestingly, the MNSCs isolated from neurofibroma tissues of the same patient with different phenotypes presented site-specific capabilities. Moreover, different levels of brain-derived neurotrophic factor (BDNF) in neurofibroma tissues can impact the MNSCs by activating the TrkB/p38 MAPK pathway. Systemic administration of BDNF neutralizing antibodies inhibited MNSCs’ characteristics. Conclusions: We demonstrated that BDNF can modulate MNSCs and thereby controlling different tumor phenotypes between the head and trunk regions. Application of BDNF neutralizing antibodies may inhibit p38 MAPK pathway, therefore providing a promising strategy for managing NF1.

With the development of the Low Earth Orbit (LEO) communication constellations, it has become a hot area of research to provide additional navigation augmentation services. Limited by volume, weight, power consumption, and running time, the in-flight performance of navigation augmentation payload remains to be investigated. In this paper, we analyze the data quality of on-board GNSS observation and evaluate the precision of short-arc dynamic Precise Orbit Determination (POD) performance based on the WangTong-01 (WT01) mission. Furthermore, the downlink navigation measurement data of WT01 satellites are analyzed and compared with the GNSS observations. The results show that the average multipath errors of the WT01 on-board GPS L1, L2 and BeiDou Satellite Navigation System (BDS) B1, B2 code observation are 0.54, 0.74, 0.65, and 0.58 m, respectively. The short-arc dynamic POD three-dimensional (3D) overlapping accuracy is 7.1 cm. The average multipath errors of downlink navigation signal Z1 and Z2 are 0.81 and 0.80 m, respectively, which at the same order of magnitude as GNSS signals. The maximum Carrier-to-Noise Ratio (C/N0) value of WT01 downlink measurement data can reach 60 dB Hz, which is much stronger than GNSS and indicates the navigation signals of LEO satellites can meet the basic requirement of navigation augmentation.

Bone resorption occurs after bone grafting, however, contemporaneous reconstruction of the innervation of the bone graft is a potential treatment to maintain the bone mass of the graft. The innervation of bone is an emerging research topic. To understand the potential molecular mechanisms of bone innervation after bone grafting, we collected normal iliac bone tissue as well as bone grafts with or without innervation from nine patients 1 year after surgery and performed RNA sequencing. We identified differentially expressed genes) from these samples and used the gene ontology and Kyoto Encyclopedia of Genes and Genomes databases for functional enrichment and signaling pathway analysis. In parallel, we established protein–protein interaction networks to screen functional modules. Based on bioinformatic results, we validated in vitro the osteogenic differentiation potential of rat bone marrow mesenchymal stem cells (BMMSCs) after calcitonin gene-related peptide (CGRP) stimulation and the expression of p38 MAPK and Wnt6/β-catenin pathways during osteogenesis. Our transcriptome analysis of bone grafts reveals functional modules and signaling pathways of innervation which play a vital role in the structural and functional integration of the bone graft. Simultaneously, we demonstrate that CGRP regulates the differentiation of BMMSCs through p38 MAPK and Wnt6/β-catenin.

Advancement of materials along with their fascinating properties play increasingly important role in facilitating the rapid progress in medicine. An excellent example is the recent development of biosensors based on nanomaterials that induce surface plasmon effect for screening biomarkers of various diseases ranging from cancer to Covid-19. The recent global pandemic re-confirmed the trend of real-time diagnosis in public health to be in point-of-care (POC) settings that can screen interested biomarkers at home, or literally anywhere else, at any time. Plasmonic biosensors, thanks to its versatile designs and extraordinary sensitivities, can be scaled into small and portable devices for POC diagnostic tools. In the meantime, efforts are being made to speed up, simplify and lower the cost of the signal readout process including converting the conventional heavy laboratory instruments into lightweight handheld devices. This article reviews the recent progress on the design of plasmonic nanomaterial-based biosensors for biomarker detection with a perspective of POC applications. After briefly introducing the plasmonic detection working mechanisms and devices, the selected highlights in the field focusing on the technology's design including nanomaterials development, structure assembly, and target applications are presented and analyzed. In parallel, discussions on the sensor's current or potential applicability in POC diagnosis are provided. Finally, challenges and opportunities in plasmonic biosensor for biomarker detection, such as the current Covid-19 pandemic and its testing using plasmonic biosensor and incorporation of machine learning algorithms are discussed.

Bone defects caused by various factors may cause morphological and functional disorders that can seriously affect patient’s quality of life. Autologous bone grafting is morbid, involves numerous complications, and provides limited volume at donor site. Hence, tissue-engineered bone is a better alternative for repair of bone defects and for promoting a patient’s functional recovery. Besides good biocompatibility, scaffolding materials represented by hydroxyapatite (HA) composites in tissue-engineered bone also have strong ability to guide bone regeneration. The development of manufacturing technology and advances in material science have made HA composite scaffolding more closely related to the composition and mechanical properties of natural bone. The surface morphology and pore diameter of the scaffold material are more important for cell proliferation, differentiation, and nutrient exchange. The degradation rate of the composite scaffold should match the rate of osteogenesis, and the loading of cells/cytokine is beneficial to promote the formation of new bone. In conclusion, there is no doubt that a breakthrough has been made in composition, mechanical properties, and degradation of HA composites. Biomimetic tissue-engineered bone based on vascularization and innervation show a promising future.

In recent years, the multi-GNSS positioning application is becoming more and more popular, same to the low Earth orbit (LEO) satellite precise orbit determination (POD) based on the onboard multi-GNSS measurements. The third-generation Beidou navigation satellite system (BDS-3) provides a new option to obtain the LEO satellite orbit solutions. However, the receiver intersystem bias (ISB) of different GNSS is unavoidable in multi-GNSS data processing. This paper’s main goal is absorption of the impact of the ISB between BDS-3 and BDS-2 on the LEO satellite POD. Taking GPS-based POD solutions for the reference orbit, this paper evaluates the orbit accuracy of BDS-2-based POD, BDS-3-based POD, BDS-2 and BDS-3 combined PODs with/without ISB. The BDS-3-based POD accuracy is 6.57 cm in the 3D direction, a 56% improvement over BDS-2-based POD. When the ISB between BDS-3 and BDS-2 is estimated, the BDS-2/3 combined POD accuracy of 5.37 cm in the 3D direction is better than that without ISB, which is a 64% improvement over BDS-2-based POD and 18% improvement over BDS-3-based POD. For GPS and BDS-2/3 combined POD, the GPS and BDS-3 joint POD solutions have the smallest RMS differences in overlapping consistency and smallest RMS differences compared to GPS-based POD. This study indicates that estimating the BDS-2/3 receiver ISB in BDS-2/3 joint POD could improve the orbit accuracy, and the GPS and BDS-3 joint POD solution is better than another combined POD. This paper will provide meaningful references for the LEO satellite multi-GNSS-based POD.