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The importance of daily data on reference evapotranspiration (ET 0 ) has increased in recent years due to its relevance in planning and decision making regarding irrigated agriculture, water production, and forest restoration. Facing the scarcity of this information measured in loco, the study of interpolation methods capable of representing ET 0 becomes important. Therefore, this study aimed to evaluate the adequacy of the Random Forest (RF) method in the spatialization of ET 0 in the watersheds of the Mid-South region of the Espírito Santo State, located within the Atlantic Forest biome, Brazil. From this study, it was found that the RF method is the most suitable one for ET 0 spatialization when compared to the Angular distance weighting (ADW) and the inverse distance weighting (IDW) techniques. Also, the spatializations carried out by this method were transformed into databases in a grid format and made available online. Furthermore, the RF database was also compared to other ET 0 grid databases, and it was concluded that the RF database also carried out a better performance than the other ones., (© 2022. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)