125 results on '"Yun, Guan"'
Search Results
2. Upregulation of circ0000381 attenuates microglial/macrophage pyroptosis after spinal cord injury
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Yan Zhang, Wenkai Zhang, Tao Liu, Ziqian Ma, Wenxiu Zhang, Yun Guan, and Xueming Chen
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circ0000381 ,inflammasome ,macrophage ,microglia ,mir-423-3p ,neuroinflammation ,neuroprotection ,nlrp3 ,pyroptosis ,rna-seq ,spinal cord injury ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
[INLINE:1] Neuroinflammation exacerbates secondary damage after spinal cord injury, while microglia/macrophage pyroptosis is important to neuroinflammation. Circular RNAs (circRNAs) play a role in the central nervous system. However, the functional role and mechanism of circRNAs in regulating microglia/macrophage pyroptosis after spinal cord injury are still poorly studied. In the present study, we detected microglia/macrophage pyroptosis in a female rat model of spinal cord injury, along with upregulated levels of circ0000381 in the spinal cord. Our further experimental results suggest that circ0000381 may function as a sponge to sequester endogenous microRNA423-3p (miR-423-3p), which can increase the expression of NOD-like receptor 3 (NLRP3), a pyroptosis marker. Therefore, upregulation of circ0000381 may be a compensatory change after spinal cord injury to attenuate microglia/macrophage pyroptosis. Indeed, knockdown of circ0000381 expression exacerbated microglia/macrophage pyroptosis. Collectively, our findings provide novel evidence for the upregulation of circ0000381, which may serve as a neuroprotective mechanism to attenuate microglia/macrophage pyroptosis after spinal cord injury. Accordingly, circ0000381 may be a novel therapeutic target for the treatment of spinal cord injury.
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- 2024
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3. A GC–MS-based untargeted metabolomics approach for comprehensive metabolic profiling of mycophenolate mofetil-induced toxicity in mice
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Tongfeng Zhao, Yaxin Zhao, Haotian Chen, Wenxue Sun, and Yun Guan
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mycophenolate mofetil ,toxicity ,gas chromatography-mass spectrometry ,multiparameter flow cytometry analysis ,lymphocytes ,Biology (General) ,QH301-705.5 - Abstract
Background: Mycophenolate mofetil (MMF), the morpholinoethyl ester of mycophenolic acid, is widely used for maintenance immunosuppression in transplantation. The gastrointestinal toxicity of MMF has been widely uncovered. However, the comprehensive metabolic analysis of MMF-induced toxicity is lacking. This study is aimed to ascertain the metabolic changes after MMF administration in mice.Methods: A total of 700 mg MMF was dissolved in 7 mL dimethyl sulfoxide (DMSO), and then 0.5 mL of mixture was diluted with 4.5 mL of saline (100 mg/kg). Mice in the treatment group (n = 9) were given MMF (0.1 mL/10 g) each day via intraperitoneal injection lasting for 2 weeks, while those in the control group (n = 9) received the same amount of blank solvent (DMSO: saline = 1:9). Gas chromatography-mass spectrometry was utilized to identify the metabolic profiling in serum samples and multiple organ tissues of mice. The potential metabolites were identified using orthogonal partial least squares discrimination analysis. Meanwhile, we used the MetaboAnalyst 5.0 (http://www.metaboanalyst.ca) and Kyoto Encyclopedia of Genes and Genomes database (http://www.kegg.jp) to depict the metabolic pathways. The percentages of lymphocytes in spleens were assessed by multiparameter flow cytometry analysis.Results: Compared to the control group, we observed that MMF treatment induced differential expression of metabolites in the intestine, hippocampus, lung, liver, kidney, heart, serum, and cortex tissues. Subsequently, we demonstrated that multiple amino acids metabolism and fatty acids biosynthesis were disrupted following MMF treatment. Additionally, MMF challenge dramatically increased CD4+ T cell percentages but had no significant influences on other types of lymphocytes.Conclusion: MMF can affect the metabolism in various organs and serum in mice. These data may provide preliminary judgement for MMF-induced toxicity and understand the metabolic mechanism of MMF more comprehensively.
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- 2024
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4. CL-ADDA: Contrastive Learning with Amplitude-Driven Data Augmentation for fMRI-Based Individualized Predictions.
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Jiangcong Liu, Le Xu, Yun Guan, Hao Ma, and Lixia Tian
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- 2023
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5. Chronic pain after spine surgery: Insights into pathogenesis, new treatment, and preventive therapy
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Qichao Wu, Xiang Cui, Leo C. Guan, Chi Zhang, Jing Liu, Neil C. Ford, Shaoqiu He, Xueming Chen, Xu Cao, Lei Zang, and Yun Guan
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Animal models ,Chronic pain after spine surgery ,Failed back surgery syndrome ,Low back pain ,Peripheral nervous system ,Sensitization ,Diseases of the musculoskeletal system ,RC925-935 - Abstract
Chronic pain after spine surgery (CPSS) is often characterized by intractable low back pain and/or radiating leg pain, and has been reported in 8–40% of patients that received lumbar spine surgery. We conducted a literature search of PubMed, MEDLINE/OVID with a focus on studies about the etiology and treatments of CPSS and low back pain. Our aim was to provide a narrative review that would help us better understand the pathogenesis and current treatment options for CPSS. This knowledge will aid in the development of optimal strategies for managing postoperative pain symptoms and potentially curing the underlying etiologies. Firstly, we reviewed recent advances in the mechanistic study of CPSS, illustrated both structural (e.g., fibrosis and scaring) and non-structural factors (e.g., inflammation, neuronal sensitization, glial activation, psychological factor) causing CPSS, and highlighted those having not been given sufficient attention as the etiology of CPSS. Secondly, we summarized clinical evidence and therapeutic perspectives of CPSS. We also presented new insights about the treatments and etiology of CPSS, in order to raise awareness of medical staff in the identification and management of this complex painful disease. Finally, we discussed potential new targets for clinical interventions of CPSS and future perspectives of mechanistic and translational research. CPSS patients often have a mixed etiology. By reviewing recent findings, the authors advocate that clinicians shall comprehensively evaluate each case to formulate a patient-specific and multi-modal pain treatment, and importantly, consider an early intraoperative intervention that may decrease the risk or even prevent the onset of CPSS. Translational potential statement: CPSS remains difficult to treat. This review broadens our understanding of clinical therapies and underlying mechanisms of CPSS, and provides new insights which will aid in the development of novel mechanism-based therapies for not only managing the established pain symptoms but also preventing the development of CPSS.
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- 2023
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6. Moderate white light exposure enhanced spatial memory retrieval by activating a central amygdala-involved circuit in mice
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MengJuan Shang, MeiLun Shen, RuoTong Xu, JingYu Du, JiMeng Zhang, Ding OuYang, JunZe Du, JunFeng Hu, ZhiChuan Sun, BingXia Wang, Qian Han, Yang Hu, YiHong Liu, Yun Guan, Jing Li, GuoZhen Guo, and JunLing Xing
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Biology (General) ,QH301-705.5 - Abstract
Abstract Light exposure can profoundly affect neurological functions and behaviors. Here, we show that short-term exposure to moderate (400 lux) white light during Y-maze test promoted spatial memory retrieval and induced only mild anxiety in mice. This beneficial effect involves the activation of a circuit including neurons in the central amygdala (CeA), locus coeruleus (LC), and dentate gyrus (DG). Specifically, moderate light activated corticotropin-releasing hormone (CRH) positive (+) CeA neurons and induced the release of corticotropin-releasing factor (CRF) from their axon terminals ending in the LC. CRF then activated tyrosine hydroxylase-expressing LC neurons, which send projections to DG and release norepinephrine (NE). NE activated β-adrenergic receptors on CaMKIIα-expressing DG neurons, ultimately promoting spatial memory retrieval. Our study thus demonstrated a specific light scheme that can promote spatial memory without excessive stress, and unraveled the underlying CeA-LC-DG circuit and associated neurochemical mechanisms.
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- 2023
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7. FXR agonist GW4064 enhances anti-PD-L1 immunotherapy in colorectal cancer
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Lu Lu, Yi-Xin Jiang, Xiao-Xia Liu, Jin-Mei Jin, Wen-Jie Gu, Xin Luan, Ying-Yun Guan, and Li-Jun Zhang
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Colorectal cancer ,farnesoid X receptor ,GW4064 ,PD-L1 ,Immunologic diseases. Allergy ,RC581-607 ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
ABSTRACTColorectal cancer (CRC) is one of the top three malignant tumors in terms of morbidity, and the limited efficacy of existing therapies urges the discovery of potential treatment strategies. Immunotherapy gradually becomes a promising cancer treatment method in recent decades; however, less than 10% of CRC patients could really benefit from immunotherapy. It is pressing to explore the potential combination therapy to improve the immunotherapy efficacy in CRC patients. It is reported that Farnesoid X receptor (FXR) is deficiency in CRC and associated with immunity. Herein, we found that GW4064, a FXR agonist, could induce apoptosis, block cell cycle, and mediate immunogenic cell death (ICD) of CRC cells in vitro. Disappointingly, GW4064 could not suppress the growth of CRC tumors in vivo. Further studies revealed that GW4064 upregulated PD-L1 expression in CRC cells via activating FXR and MAPK signaling pathways. Gratifyingly, the combination of PD-L1 antibody with GW4064 exhibited excellent anti-tumor effects in CT26 xenograft models and increased CD8+ T cells infiltration, with 33% tumor bearing mice cured. This paper illustrates the potential mechanisms of GW4064 to upregulate PD-L1 expression in CRC cells and provides important data to support the combination therapy of PD-L1 immune checkpoint blockade with FXR agonist for CRC patients.
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- 2023
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8. BrainNet with Connectivity Attention for Individualized Predictions Based on Multi-Facet Connections Extracted from Resting-State fMRI Data.
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Hao Ma, Fan Wu, Yun Guan, Le Xu, Jiangcong Liu, and Lixia Tian
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- 2023
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9. A Siamese Network With Node Convolution for Individualized Predictions Based on Connectivity Maps Extracted From Resting-State fMRI Data.
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Le Xu, Hao Ma, Yun Guan, Jiangcong Liu, Huifang Huang, Yang Zhang, and Lixia Tian
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- 2023
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10. Dosimetric quality of HyperArc in boost radiotherapy for single glioblastoma: comparison with CyberKnife and manual VMAT
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Mingyuan Pan, Wenqian Xu, Lei Sun, Chaozhuang Wang, Shengnan Dong, Yun Guan, Jun Yang, and Enmin Wang
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Stereotactic radiotherapy ,Dosimetry ,CyberKnife ,HyperArc ,Glioblastoma ,Medical physics. Medical radiology. Nuclear medicine ,R895-920 ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background Stereotactic radiotherapy (SRT) and hypo-fractionated radiotherapy are feasible treatment options for single glioblastoma multiforme when combined with conventional radiotherapy or delivered alone. HyperArc (HA), a novel linac-based method with 4 noncoplanar arcs, has been introduced into stereotactic radiosurgery (SRS) for single and multiple metastases. In this study, we compared the dosimetric quality of HyperArc with the well-established CyberKnife (CK) and conventional VMAT methods of SRT for a single, large target. Methods Sixteen patients treated in our center with their clinical CK plans were enrolled, and the linac-based plans were designed in silico. From the aspect of normal tissue protection and treatment efficacy, we compared the conformity index (CI), gradient index (GI), homogeneity index (HI), dose distribution in planning target volume, dose in the normal brain tissue, and mean dose of several organs at risk (OARs). All of the data were evaluated with nonparametric Kruskal‒Wallis tests. We further investigated the relationship of the dose distribution with the tumor volume and its location. Results The results showed that with a higher CI (0.94 ± 0.03) and lower GI (2.57 ± 0.53), the HA plans generated a lower dose to the OARs and the normal tissue. Meanwhile, the CK plans achieved a higher HI (0.35 ± 0.10) and generated a higher dose inside the tumor. Although manual VMAT showed slight improvement in dose quality and less monitoring units (2083 ± 225), HA can save half of the delivery time of CK (37 minutes) on average. Conclusion HA plans have higher conformity and spare OARs with lower normal tissue irradiation, while CK plans achieve a higher mean dose in tumors. HA with 4 arcs is sufficient in dosimetric quality for a single tumor with great convenience in planning and treatment processes compared with conventional VMAT. The tumor size and location are factors to be considered when selecting treatment equipment.
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- 2023
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11. Genetic polymorphism of clopidogrel metabolism related gene CYP2C19 gene in Chinese from Foshan area of Guangdong Province
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Xiao-wen Yuan, Shi-yun Yuan, Guo-xin Wu, zhi-xin Wu, and Zi-yun Guan
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Polymorphism ,genetic ,pharmacogenetics ,CYP2C19 ,China ,Diseases of the blood and blood-forming organs ,RC633-647.5 - Abstract
Background: The CYP2C19 gene is highly polymorphic, and CYP2C19 is involved in the broad interindividual variability of the clinical efficacy of certain clinical medications, such as clopidogrel. However, data on the CYP2C19 genotype in the Chinese population of the Foshan area of Guangdong Province are scarce. The purpose of this study was to determine CYP2C19 genetic polymorphisms in patients in the Foshan area and to compare the CYP2C19 genotype frequencies in different populations to determine the allele distribution pattern to identify the most appropriate prescription.Methods: The CYP2C19 gene was detected in 1231 patients on a gene chip platform, and the genotype frequencies of CYP2C19 in Foshan populations from different populations were compared.Results: The frequencies of CYP2C19*1, *2 and *3 in the Foshan population were 63.89%, 30.46% and 5.65%, respectively. For the three metabolic types, the frequency associated with the rapid metabolism type (*1/*1) was 41.51 [95% confidence interval (CI) 40.11 to 42.91%]; that for the intermediate metabolism type (*1/*2, *1/*3) was 44.76% (95% CI 43.34 to 46.18) and that for the slow metabolism type (*2/*2, *2/*3, *3/*3) was 13.73% (95% CI 12.75 to 14.71%). In the Foshan population, the frequencies of the CYP2C19 *2 and *3 alleles were similar to those previously reported for Chinese and other Asian populations.Conclusion: Our study is a report on the genetic basis of CYP2C19 polymorphism in the Foshan population. Our results will potentially contribute to the improvement of pharmacotherapy effectiveness by providing personalized medicine for the Foshan population.
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- 2022
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12. A phase II open label, single arm study of hypofractionated stereotactic radiotherapy with chemoradiotherapy using intensity-modulated radiotherapy for newly diagnosed glioblastoma after surgery: the HSCK-010 trial protocol
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Yun Guan, Mingyuan Pan, Jun Yang, Qiuxia Lu, Liangfu Han, Ying Liu, Jing Li, Huaguang Zhu, Xiu Gong, Guanghai Mei, Xiaoxia Liu, Li Pan, Jiazhong Dai, Yang Wang, Enmin Wang, and Xin Wang
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Hypofractionated stereotactic radiotherapy ,Newly diagnosed glioblastoma ,Adjuvant chemoradiotherapy ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background The most frequently diagnosed primary brain tumor is glioblastoma (GBM). Nearly all patients experience tumor recurrence and up to 90% of which is local recurrence. Thus, increasing the therapeutic ratio of radiotherapy using hypofractionated stereotactic radiotherapy (HSRT) can reduce treatment time and may increase tumor control and improve survival. To evaluate the efficacy and toxicity of the combination of HSRT and intensity-modulated radiotherapy (IMRT) with temozolomide after surgery in GBM patients and provide evidence for further randomized controlled trials. Methods/design HSCK-010 is an open-label, single-arm phase II trial (NCT04547621) which includes newly diagnosed GBM patients who underwent gross total resection. Patients will receive the combination of 30 Gy/5fx HSRT, and 20 Gy/10fx IMRT adjuvant therapy with concurrent temozolomide and adjuvant chemotherapy. The primary endpoint is overall survival (OS). Secondary outcomes include progression-free survival (PFS) rate, objective-response rate (ORR), quality of life (Qol) before and after the treatment, cognitive function before and after the treatment, and rate of treatment-related adverse events (AE). The combination of HSRT and IMRT with temozolomide can benefit the patients after surgery with good survival, acceptable toxicity, and reduced treatment time. Trial registration NCT04547621 . Registered on 14 September 2020.
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- 2022
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13. Mechanisms of bone pain: Progress in research from bench to bedside
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Gehua Zhen, Yuhan Fu, Chi Zhang, Neil C. Ford, Xiaojun Wu, Qichao Wu, Dong Yan, Xueming Chen, Xu Cao, and Yun Guan
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Biology (General) ,QH301-705.5 ,Physiology ,QP1-981 - Abstract
Abstract The field of research on pain originating from various bone diseases is expanding rapidly, with new mechanisms and targets asserting both peripheral and central sites of action. The scope of research is broadening from bone biology to neuroscience, neuroendocrinology, and immunology. In particular, the roles of primary sensory neurons and non-neuronal cells in the peripheral tissues as important targets for bone pain treatment are under extensive investigation in both pre-clinical and clinical settings. An understanding of the peripheral mechanisms underlying pain conditions associated with various bone diseases will aid in the appropriate application and development of optimal strategies for not only managing bone pain symptoms but also improving bone repairing and remodeling, which potentially cures the underlying etiology for long-term functional recovery. In this review, we focus on advances in important preclinical studies of significant bone pain conditions in the past 5 years that indicated new peripheral neuronal and non-neuronal mechanisms, novel targets for potential clinical interventions, and future directions of research.
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- 2022
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14. The future of therapeutic peripheral nerve stimulation for chronic pain.
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Christine Beauchene, Claire A. Zurn, Wanru Duan, Yun Guan, and Sridevi V. Sarma
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- 2022
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15. Genetic etiology study in a large cohort with congenital insensitivity to pain with anhidrosis.
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Shuang Li, Xiuzhi Ren, Yun Guan, Feiyue Zhao, Yixuan Cao, Xingzhu Geng, Yanzhou Wang, Nan Wu, Lingqian Wu, and Xiuli Zhao
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- 2024
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16. Effects of Early Exposure to Isoflurane on Susceptibility to Chronic Pain Are Mediated by Increased Neural Activity Due to Actions of the Mammalian Target of the Rapamycin Pathway
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Qun Li, Reilley Paige Mathena, Fengying Li, Xinzhong Dong, Yun Guan, and Cyrus David Mintz
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anesthesia neurotoxicity ,neuropathic pain ,neural activity ,mammalian target of rapamycin (mTOR) ,dorsal spinal cord (DSC) ,dorsal root ganglion (DRG) ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Patients who have undergone surgery in early life may be at elevated risk for suffering neuropathic pain in later life. The risk factors for this susceptibility are not fully understood. Here, we used a mouse chronic pain model to test the hypothesis that early exposure to the general anesthetic (GA) Isoflurane causes cellular and molecular alterations in dorsal spinal cord (DSC) and dorsal root ganglion (DRG) that produces a predisposition to neuropathic pain via an upregulation of the mammalian target of the rapamycin (mTOR) signaling pathway. Mice were exposed to isoflurane at postnatal day 7 (P7) and underwent spared nerve injury at P28 which causes chronic pain. Selected groups were treated with rapamycin, an mTOR inhibitor, for eight weeks. Behavioral tests showed that early isoflurane exposure enhanced susceptibility to chronic pain, and rapamycin treatment improved outcomes. Immunohistochemistry, Western blotting, and q-PCR indicated that isoflurane upregulated mTOR expression and neural activity in DSC and DRG. Accompanying upregulation of mTOR and rapamycin-reversible changes in chronic pain-associated markers, including N-cadherin, cAMP response element-binding protein (CREB), purinergic P2Y12 receptor, glial fibrillary acidic protein (GFAP) in DSC; and connexin 43, phospho-extracellular signal-regulated kinase (p-ERK), GFAP, Iba1 in DRG, were observed. We concluded that early GA exposure, at least with isoflurane, alters the development of pain circuits such that mice are subsequently more vulnerable to chronic neuropathic pain states.
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- 2023
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17. Inhibition of the LRRC8A channel promotes microglia/macrophage phagocytosis and improves outcomes after intracerebral hemorrhagic stroke
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Jing Liu, Danmin Shen, Chao Wei, Weihua Wu, Zhaoli Luo, Liye Hu, Zhongnan Xiao, Tingting Hu, Qingyu Sun, Xiaotong Wang, Yumeng Ding, Meng Liu, Miaoyi Pang, Kaiyuan Gai, Yiran Ma, Yichen Tian, Yan Yu, Peipei Wang, Yun Guan, Meng Xu, Fei Yang, and Qian Li
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Immunology and neurology ,Science - Abstract
Summary: Promoting microglial/macrophage (M/Mφ) phagocytosis accelerates hematoma clearance and improves the prognosis of intracerebral hemorrhagic stroke (ICH). Cation channels such as Piezo1 modulate bacterial clearance by regulating M/Mφ. Whether LRRC8A, an anion channel, affects M/Mφ erythrophagocytosis and functional recovery after ICH was investigated here. We found that LRRC8A is highly expressed on M/Mφ in the perihematomal region of ICH mice. Conditional knockout of Lrrc8a in M/Mφ or treatment with an LRRC8A channel blocker accelerated hematoma clearance, reduced neuronal death, and improved functional recovery after ICH. Mechanistically, the LRRC8A channel inhibition promoted M/Mφ phagocytosis by activating AMP-activated protein kinase (AMPK), thereby inducing nuclear translocation of nuclear factor-erythroid 2 related factor 2 (Nrf2) and increasing Cd36 transcription. Our findings illuminate the regulation of M/Mφ phagocytosis by the LRRC8A channel via the AMPK-Nrf2-CD36 pathway after ICH, suggesting that LRRC8A is a potential target for hematoma clearance in ICH treatment.
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- 2022
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18. scRNA-sequencing reveals subtype-specific transcriptomic perturbations in DRG neurons of PirtEGFPf mice in neuropathic pain condition
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Chi Zhang, Ming-Wen Hu, Xue-Wei Wang, Xiang Cui, Jing Liu, Qian Huang, Xu Cao, Feng-Quan Zhou, Jiang Qian, Shao-Qiu He, and Yun Guan
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single-cell RNA-sequencing ,neuropathic pain ,mouse ,dorsal root ganglion ,nerve injury ,axon regeneration ,Medicine ,Science ,Biology (General) ,QH301-705.5 - Abstract
Functionally distinct subtypes/clusters of dorsal root ganglion (DRG) neurons may play different roles in nerve regeneration and pain. However, details about their transcriptomic changes under neuropathic pain conditions remain unclear. Chronic constriction injury (CCI) of the sciatic nerve represents a well-established model of neuropathic pain, and we conducted single-cell RNA-sequencing (scRNA-seq) to characterize subtype-specific perturbations of transcriptomes in lumbar DRG neurons on day 7 post-CCI. By using PirtEGFPf mice that selectively express an enhanced green fluorescent protein in DRG neurons, we established a highly efficient purification process to enrich neurons for scRNA-seq. We observed the emergence of four prominent CCI-induced clusters and a loss of marker genes in injured neurons. Importantly, a portion of injured neurons from several clusters were spared from injury-induced identity loss, suggesting subtype-specific transcriptomic changes in injured neurons. Moreover, uninjured neurons, which are necessary for mediating the evoked pain, also demonstrated cell-type-specific transcriptomic perturbations in these clusters, but not in others. Notably, male and female mice showed differential transcriptomic changes in multiple neuronal clusters after CCI, suggesting transcriptomic sexual dimorphism in DRG neurons after nerve injury. Using Fgf3 as a proof-of-principle, RNAscope study provided further evidence of increased Fgf3 in injured neurons after CCI, supporting scRNA-seq analysis, and calcium imaging study unraveled a functional role of Fgf3 in neuronal excitability. These findings may contribute to the identification of new target genes and the development of DRG neuron cell-type-specific therapies for optimizing neuropathic pain treatment and nerve regeneration.
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- 2022
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19. Effects of immunoglobulin Y-loaded amorphous calcium phosphate on dentinal tubules occlusion and antibacterial activity
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Yanhong Yan, Yun Guan, Linjuan Luo, Bingqiang Lu, Feng Chen, and Beizhan Jiang
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dentin hypersensitivity ,amorphous calcium phosphate ,mineralization ,antibacterial activity ,IgY ,Biotechnology ,TP248.13-248.65 - Abstract
Aim: This study aimed to evaluate the effects of immunoglobulin Y (IgY)-loaded amorphous calcium phosphate (ACP) (IgY@ACP) on dentinal tubule occlusion and antibacterial activity.Methodology: IgY@ACP was synthesized based on a biomimetic mineralization strategy. The structure was examined by transmission electron microscopy and Fourier transform infrared spectroscopy. The IgY release property was assessed in vitro. The cell biocompatibility of IgY@ACP was evaluated by CCK-8. The dentin disks were prepared using healthy human molars, and their dentinal tubules were exposed to EDTA. Subsequently, they were randomly selected and treated with or without IgY@ACP for 7 days. The tubule occlusion morphologies and newly formed layers were observed by scanning electron microscopy (SEM) and x-ray diffraction, respectively. To evaluate the acid resistance and abrasion resistance of IgY@ACP, dentin disks that were treated for 1 day were immersed in acid solution or subjected to a toothbrush. The antibacterial effects against Streptococcus mutans (S. mutans) were evaluated by colony-forming unit (CFU) counting, adhesion property assessment, and crystal violet and live/dead bacterial staining. Finally, the occlusion effect was evaluated in rat incisors in vivo. One-way analysis of variance (ANOVA) was performed for statistical analysis. The level of significance was set at 0.05.Results: IgY@ACP presented an amorphous phase with a nanosize (60–80 nm) and sustained release of protein within 48 h. The CCK-8 results showed that IgY@ACP had good biocompatibility. After treatment with IgY@ACP for 1 day, the majority of dentinal tubules were occluded by a 0.3-μm-thick mineralized layer. Seven days later, all dentinal tubules were occluded by mineralization with a thickness of 1.4 μm and a depth of 16 μm. The newly mineralized layer showed hydroxyapatite-like diffraction peaks. In addition, IgY@ACP had good acid and abrasion resistance. After treatment with IgY@ACP, the CFU counting and adhesion rate of S. mutans were significantly reduced, the crystal violet staining was lighter, and the S. mutans staining revealed more dead cells. Most importantly, IgY@ACP had a certain occluding property in rat incisors in vivo.Conclusion: IgY@ACP can effectively occlude dentinal tubules with acid-resistant stability and has prominent anti-S. mutans effects, rendering it a potentially suitable desensitization material in the clinic.
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- 2022
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20. Nuclear Translocation of LDHA Promotes the Catabolism of BCAAs to Sustain GBM Cell Proliferation through the TxN Antioxidant Pathway
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Zhujun Li, Zhiyan Gu, Lan Wang, Yun Guan, Yingying Lyu, Jialong Zhang, Yin Wang, Xin Wang, Ji Xiong, and Ying Liu
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lactate dehydrogenase A ,glutamate ,branched-chain amino acid transaminase 1 ,redox balance ,thioredoxin ,GBM ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Glutamate is excitotoxic to neurons. The entry of glutamine or glutamate from the blood into the brain is limited. To overcome this, branched-chain amino acids (BCAAs) catabolism replenishes the glutamate in brain cells. Branched-chain amino acid transaminase 1 (BCAT1) activity is silenced by epigenetic methylation in IDH mutant gliomas. However, glioblastomas (GBMs) express wild type IDH. Here, we investigated how oxidative stress promotes BCAAs’ metabolism to maintain intracellular redox balance and, consequently, the rapid progression of GBMs. We found that reactive oxygen species (ROS) accumulation promoted the nuclear translocation of lactate dehydrogenase A (LDHA), which triggered DOT1L (disruptor of telomeric silencing 1-like)-mediated histone H3K79 hypermethylation and enhanced BCAA catabolism in GBM cells. Glutamate derived from BCAAs catabolism participates in antioxidant thioredoxin (TxN) production. The inhibition of BCAT1 decreased the tumorigenicity of GBM cells in orthotopically transplanted nude mice, and prolonged their survival time. In GBM samples, BCAT1 expression was negatively correlated with the overall survival time (OS) of patients. These findings highlight the role of the non-canonical enzyme activity of LDHA on BCAT1 expression, which links the two major metabolic pathways in GBMs. Glutamate produced by the catabolism of BCAAs was involved in complementary antioxidant TxN synthesis to balance the redox state in tumor cells and promote the progression of GBMs.
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- 2023
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21. Loss of Astrocytic µ Opioid Receptors Exacerbates Aversion Associated with Morphine Withdrawal in Mice: Role of Mitochondrial Respiration
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Kateryna Murlanova, Yan Jouroukhin, Ksenia Novototskaya-Vlasova, Shovgi Huseynov, Olga Pletnikova, Michael J. Morales, Yun Guan, Atsushi Kamiya, Dwight E. Bergles, David M. Dietz, and Mikhail V. Pletnikov
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astrocytes ,µ opioid receptor ,morphine withdrawal ,oxidative phosphorylation ,reward ,Cytology ,QH573-671 - Abstract
Astrocytes express mu/µ opioid receptors, but the function of these receptors remains poorly understood. We evaluated the effects of astrocyte-restricted knockout of µ opioid receptors on reward- and aversion-associated behaviors in mice chronically exposed to morphine. Specifically, one of the floxed alleles of the Oprm1 gene encoding µ opioid receptor 1 was selectively deleted from brain astrocytes in Oprm1 inducible conditional knockout (icKO) mice. These mice did not exhibit changes in locomotor activity, anxiety, or novel object recognition, or in their responses to the acute analgesic effects of morphine. Oprm1 icKO mice displayed increased locomotor activity in response to acute morphine administration but unaltered locomotor sensitization. Oprm1 icKO mice showed normal morphine-induced conditioned place preference but exhibited stronger conditioned place aversion associated with naloxone-precipitated morphine withdrawal. Notably, elevated conditioned place aversion lasted up to 6 weeks in Oprm1 icKO mice. Astrocytes isolated from the brains of Oprm1 icKO mice had unchanged levels of glycolysis but had elevated oxidative phosphorylation. The basal augmentation of oxidative phosphorylation in Oprm1 icKO mice was further exacerbated by naloxone-precipitated withdrawal from morphine and, similar to that for conditioned place aversion, was still present 6 weeks later. Our findings suggest that µ opioid receptors in astrocytes are linked to oxidative phosphorylation and they contribute to long-term changes associated with opioid withdrawal.
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- 2023
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22. Manifold-based unsupervised metric Learning, with applications in individualized predictions based on functional connectivity.
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Fan Wu, Hao Ma, Yun Guan, and Lixia Tian
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- 2023
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23. GRAP2: A Novel Immune-Related Prognosis Biomarker in Cervical Cancer
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Shixin Lin, Jun Zhu, Xiaoling Mao, Gang Lin, Dan Yang, Yun Guan, and Jun Gao
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cervical cancer ,grap2 ,tumor microenvironment ,immunotherapy ,prognostic biomarker ,Gynecology and obstetrics ,RG1-991 - Abstract
Background: Immune infiltration of the tumor microenvironment offers unlimited possibilities for therapeutic strategies in cervical cancer, where GRAP2 is an adaptor protein engaged in diverse signal activations. However, uncertainty exists regarding GRAP2’s prognostic significance and its relationship to immune infiltration. Methods: The data on cervical cancer cases were downloaded from The Cancer Genome Atlas (TCGA) database. The ESTIMATE computational technique was utilized to calculate the amount of immunological and stromal components, which helped us to identify the differential expression genes (DEGs). Among them, GRAP2 was considered to be related to overall survival based on a protein-protein interaction network and a univariate Cox regression analysis. Thus, based on the Gene Expression Omnibus (GEO) and TCGA databases, we evaluated GRAP2’s influence on clinical prognosis. Furthermore, GRAP2 expression was analyzed by Gene Set Enrichment Analysis (GSEA). Finally, we used CIBERSORTx analysis to assess the proportion of tumor-infiltrating immune cells (TICs) and the connection between GRAP2 and the tumor immune microenvironment. Results: ESTIMATEScore was associated with cervical cancer patient’s prognosis. There are 791 DEGs and 11 potential key genes were identified including GRAP2. In survival analyses with clinical information, We found that the GRAP2 high expression group exhibited a significantly longer overall survival (OS) than the low expression group and that the gene expression gradually declined as the Federation of International of Gynecologists and Obstetricians (FIGO) stage and M classification increased. GRAP2 was strongly linked with immunity and metabolism, according to GSEA. Finally, we discovered that 11 different TIC types and GRAP2 expressions were linked. Conclusions: GRAP2 may be a novel immune-related prognosis biomarker in cervical cancer.
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- 2023
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24. Conditioned medium from human dental pulp stem cells treats spinal cord injury by inhibiting microglial pyroptosis.
- Author
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Tao Liu, Ziqian Ma, Liang Liu, Yilun Pei, Qichao Wu, Songjie Xu, Yadong Liu, Nan Ding, Yun Guan, Yan Zhang, and Xueming Chen
- Published
- 2024
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25. Long-term clinical outcomes of hypofractionated stereotactic radiotherapy using the CyberKnife robotic radiosurgery system for jugular foramen schwannomas.
- Author
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Wei Zou, Yun Guan, Huaguang Zhu, Xiu Gong, Enmin Wang, Chengjun Yao, and Xin Wang
- Published
- 2024
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26. Murine CXCR3+CXCR6+γδT Cells Reside in the Liver and Provide Protection Against HBV Infection
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Yanan Wang, Yun Guan, Yuan Hu, Yan Li, Nan Lu, and Cai Zhang
- Subjects
liver ,γδT cells ,residency ,liver-resident γδT cells ,chemotaxis ,HBV infection ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Gamma delta (γδ) T cells play a key role in the innate immune response and serve as the first line of defense against infection and tumors. These cells are defined as tissue-resident lymphocytes in skin, lung, and intestinal mucosa. They are also relatively abundant in the liver; however, little is known about the residency of hepatic γδT cells. By comparing the phenotype of murine γδT cells in liver, spleen, thymus, and small intestine, a CXCR3+CXCR6+ γδT-cell subset with tissue-resident characteristics was found in liver tissue from embryos through adults. Liver sinusoidal endothelial cells mediated retention of CXCR3+CXCR6+ γδT cells through the interactions between CXCR3 and CXCR6 and their chemokines. During acute HBV infection, CXCR3+CXCR6+ γδT cells produced high levels of IFN-γ and adoptive transfer of CXCR3+CXCR6+ γδT cells into acute HBV-infected TCRδ−/− mice leading to lower HBsAg and HBeAg expression. It is suggested that liver resident CXCR3+CXCR6+ γδT cells play a protective role during acute HBV infection. Strategies aimed at expanding and activating liver resident CXCR3+CXCR6+ γδT cells both in vivo or in vitro have great prospects for use in immunotherapy that specifically targets acute HBV infection.
- Published
- 2022
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27. Monosaccharide Composition and In Vitro Activity to HCT-116 Cells of Purslane Polysaccharides after a Covalent Chemical Selenylation
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Ling-Yu Li, Qing-Yun Guan, Ya-Ru Lin, Jun-Ren Zhao, Li Wang, Qiang Zhang, Hong-Fang Liu, and Xin-Huai Zhao
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purslane ,polysaccharide ,selenylation ,monosaccharide composition ,HCT-116 cells ,nutraceutical effect ,Chemical technology ,TP1-1185 - Abstract
The anti-cancer effects of selenylated plant polysaccharides are a focus of research. As a natural plant with extensive biological effects, there have been few studies related to edible purslane (Portulaca oleracea L.). Thus, in this study, soluble P. oleracea polysaccharides (PPS) were extracted from the dried P. oleracea and then selenylated chemically using the HNO3-Na2SeO3 method to obtain two selenylated products, namely, SePPS1 and SePPS2. Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides—arabinose, fucose, and ribose—and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition. Moreover, SePPS1 and SePPS2 shared similar features with respect to monosaccharide composition and possessed higher bioactivity than PPS in human colon cancer HCT-116 cells. Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway. SePPS2 consistently showed the highest capacity to exert these observed effects on the targeted cells, suggesting that the performed chemical selenylation of PPS (in particular when higher degrees of selenylation are reached) resulted in an increase in activity in the cells. It can thus be concluded that the performed selenylation of PPS was able to incorporate inorganic Se into the final PPS products, changing their monosaccharide composition and endowing them with enhanced nutraceutical and anti-cancer effects in the colon.
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- 2022
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28. Predictions Based on Different Climate Change Scenarios: The Habitat of Typical Locust Species Is Shrinking in Kazakhstan and Xinjiang, China
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Rui Wu, Jing-Yun Guan, Jian-Guo Wu, Xi-Feng Ju, Qing-Hui An, and Jiang-Hua Zheng
- Subjects
locust ,climate change ,maximum entropy model ,China and Kazakhstan ,risk management ,Science - Abstract
Climate change, especially climate extremes, can increase the uncertainty of locust outbreaks. The Italian locust (Calliptamus italicus (Linnaeus, 1758)), Asian migratory locust (Locusta migratoria migratoria Linnaeus, 1758), and Siberian locust (Gomphocerus sibiricus (Linnaeus, 1767)) are common pests widely distributed in the semidesert grasslands of Central Asia and its surrounding regions. Predicting the geographic distribution changes and future habitats of locusts in the context of climate warming is essential to effectively prevent large and sudden locust outbreaks. In this study, the optimized maximum entropy (MaxEnt) model, employing a combination of climatic, soil, and topographic factors, was used to predict the potential fitness areas of typical locusts in the 2030s and 2050s, assuming four shared socioeconomic pathways (SSP126, SSP245, SSP370, and SSP585) in the CMIP6 model. Modeling results showed that the mean area under the curve (AUC) and true statistical skill (TSS) of the MaxEnt model reached 0.933 and 0.7651, respectively, indicating that the model exhibited good prediction performance. Our results showed that soil surface sand content, slope, mean precipitation during the hottest season, and precipitation seasonality were the key environmental variables affecting locust distribution in the region. The three locust species were mainly distributed in the upstream region of the Irtysh River, the Alatao Mountain region, the northern slopes of the Tianshan Mountains, around Sayram Lake, the eastern part of the Alakol Lake region, the Tekes River region, the western part of Ulungur Lake, the Ili River, and the upstream region of the Tarim River. According to several climate projections, the area of potential habitat for the three most common locust species will decrease by 3.9 × 104–4.6 × 104 km2 by the 2030s and by 6.4 × 104–10.6 × 104 km2 by the 2050s. As the climate becomes more extreme, the suitable area will shrink, but the highly suitable area will expand; thus, the risk of infestation should be taken seriously. Our study present a timely investigation to add to extensive literature currently appearing regarding the myriad ways climate change may affect species. While this naturally details a limited range of taxa, methods and potential impacts may be more broadly applicable to other locust species.
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- 2022
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29. CRISPR/Cas9 correction of a dominant cis‐double‐variant in <scp> COL1A1 </scp> isolated from a patient with osteogenesis imperfecta increases the osteogenic capacity of induced pluripotent stem cells
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Yixuan Cao, Lulu Li, Xiuzhi Ren, Bin Mao, Yujiao Yang, Huan Mi, Yun Guan, Shan Li, Siji Zhou, Xin Guan, Tao Yang, and Xiuli Zhao
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Endocrinology, Diabetes and Metabolism ,Orthopedics and Sports Medicine - Published
- 2023
30. BzATP Activates Satellite Glial Cells and Increases the Excitability of Dorsal Root Ganglia Neurons In Vivo
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Zhiyong Chen, Chi Zhang, Xiaodan Song, Xiang Cui, Jing Liu, Neil C. Ford, Shaoqiu He, Guangwu Zhu, Xinzhong Dong, Menachem Hanani, and Yun Guan
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satellite glial cell ,dorsal root ganglion ,purinergic receptor ,calcium imaging ,pain ,mice ,Cytology ,QH573-671 - Abstract
The purinergic system plays an important role in pain transmission. Recent studies have suggested that activation of P2-purinergic receptors (P2Rs) may be involved in neuron-satellite glial cell (SGC) interactions in the dorsal root ganglia (DRG), but the details remain unclear. In DRG, P2X7R is selectively expressed in SGCs, which closely surround neurons, and is highly sensitive to 3’-O-(4-Benzoyl) benzoyl-ATP (BzATP). Using calcium imaging in intact mice to survey a large number of DRG neurons and SGCs, we examined how intra-ganglionic purinergic signaling initiated by BzATP affects neuronal activities in vivo. We developed GFAP-GCaMP6s and Pirt-GCaMP6s mice to express the genetically encoded calcium indicator GGCaM6s in SGCs and DRG neurons, respectively. The application of BzATP to the ganglion induced concentration-dependent activation of SGCs in GFAP-GCaMP6s mice. In Pirt-GCaMP6s mice, BzATP initially activated more large-size neurons than small-size ones. Both glial and neuronal responses to BzATP were blocked by A438079, a P2X7R-selective antagonist. Moreover, blockers to pannexin1 channels (probenecid) and P2X3R (A317491) also reduced the actions of BzATP, suggesting that P2X7R stimulation may induce the opening of pannexin1 channels, leading to paracrine ATP release, which could further excite neurons by acting on P2X3Rs. Importantly, BzATP increased the responses of small-size DRG neurons and wide-dynamic range spinal neurons to subsequent peripheral stimuli. Our findings suggest that intra-ganglionic purinergic signaling initiated by P2X7R activation could trigger SGC-neuron interaction in vivo and increase DRG neuron excitability.
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- 2022
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31. A New Porphyrin‐based Covalent Organic Framework with High Iodine Capture Capacity and I‐doping Enhanced Conductivity.
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Li, Yan, Cui, Guoxin, Cai, Xue, Yun, Guan, Zhao, Yongzheng, Jiang, Li, Cui, Shuxin, Zhang, Jinghan, Liu, Minghao, Zeng, Weiqi, Wang, Zhenlu, and Jiang, Jian
- Subjects
IODINE ,METALLOPORPHYRINS ,NITROGEN analysis ,ADSORPTION capacity ,ELECTRIC properties ,UNIFORM spaces ,PHOTOELECTROCHEMISTRY - Abstract
Covalent organic frameworks (COFs) are porous organic materials with well‐defined and uniform structure. The material is an excellent candidate as a solid adsorbent for iodine adsorption. In the present study, we report the synthesis of COF with porphyrin moiety, TF‐TA‐COF, by solvothermal reaction, which was characterized by XRD, solid‐state 13C NMR, IR, TGA, and nitrogen adsorption‐desorption analysis. TF‐TA‐COF showed a high specific surface area of 443 m2 g−1, and exhibited good adsorption performance for iodine vapor, with an adsorption capacity of 2.74 g g−1. XPS and Raman spectrum indicated that a hybrid of physisorption and chemisorption took place between host COF and iodine molecules. The electric properties of iodine‐loaded TF‐TA‐COF were also studied. After doped with iodine, the conductivity of the material increased by more than 5 orders of magnitude. The photoconductivity of I2‐doped COF was also studied and TF‐TA‐COF showed doping‐enhanced photocurrent generation. [ABSTRACT FROM AUTHOR]
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- 2024
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32. Honeycomb-like hollow carbon loaded with ruthenium nanoparticles as high-performance HER electrocatalysts.
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Peng-Cheng Ji, Yang Teng, Hong-Cheng Li, Ming-Yun Guan, and Hai-Lang Jia
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- 2024
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33. Mechanically Robust Interface at Metal/Muscovite Quasi van der Waals Epitaxy
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Chen, Jia-Wei, primary, Wei, Yun-Guan, additional, Lo, Hung-Yang, additional, Lu, SiCheng, additional, Chen, Yi-Che, additional, Lei, Chi-Fong, additional, Liu, Po-Liang, additional, Yu, Pu, additional, Tsou, Nien-Ti, additional, Yasuhara, Akira, additional, Wu, Wen-Wei, additional, and Chu, Ying-Hao, additional
- Published
- 2023
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34. Identification of solvent retention capacity quantitative trait loci by combined linkage and association mapping in wheat (Triticum aestivum L.)
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Xiao‐ling, Jiang, primary, Hong‐min, Li, additional, Yu‐ling, An, additional, Ji‐shun, Zhao, additional, Yan‐yan, Geng, additional, Xiao‐jun, Li, additional, Qing‐yun, Guan, additional, Mei‐ling, Yan, additional, Ji‐chun, Tian, additional, and Jian‐sheng, Chen, additional
- Published
- 2023
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35. Modulation of Trans-Synaptic Neurexin–Neuroligin Interaction in Pathological Pain
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Huili Li, Ruijuan Guo, Yun Guan, Junfa Li, and Yun Wang
- Subjects
neurexin ,neuroligin ,pain ,synapse ,plasticity ,Cytology ,QH573-671 - Abstract
Synapses serve as the interface for the transmission of information between neurons in the central nervous system. The structural and functional characteristics of synapses are highly dynamic, exhibiting extensive plasticity that is shaped by neural activity and regulated primarily by trans-synaptic cell-adhesion molecules (CAMs). Prototypical trans-synaptic CAMs, such as neurexins (Nrxs) and neuroligins (Nlgs), directly regulate the assembly of presynaptic and postsynaptic molecules, including synaptic vesicles, active zone proteins, and receptors. Therefore, the trans-synaptic adhesion mechanisms mediated by Nrx–Nlg interaction can contribute to a range of synaptopathies in the context of pathological pain and other neurological disorders. The present review provides an overview of the current understanding of the roles of Nrx–Nlg interaction in the regulation of trans-synaptic connections, with a specific focus on Nrx and Nlg structures, the dynamic shaping of synaptic function, and the dysregulation of Nrx–Nlg in pathological pain. Additionally, we discuss a range of proteins capable of modulating Nrx–Nlg interactions at the synaptic cleft, with the objective of providing a foundation to guide the future development of novel therapeutic agents for managing pathological pain.
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- 2022
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36. Safety and Efficacy of Hypofractionated Stereotactic Radiotherapy with Anlotinib Targeted Therapy for Glioblastoma at the First Recurrence: A Preliminary Report
- Author
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Yun Guan, Jing Li, Xiu Gong, Huaguang Zhu, Chao Li, Guanghai Mei, Xiaoxia Liu, Li Pan, Jiazhong Dai, Yang Wang, Enmin Wang, Ying Liu, and Xin Wang
- Subjects
hypofractionated stereotactic radiotherapy ,recurrent high-grade glioma ,salvage treatment ,anlotinib ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
(1) Background: Hypofractionated stereotactic radiotherapy (HSRT) and anti-vascular endothelial growth factor (VEGF) antibodies have been reported to have a promising survival benefit in recent studies. Anlotinib is a new oral VEGF receptor inhibitor. This report describes our experience using HSRT and anlotinib for recurrent glioblastoma (rGBM). (2) Methods: Between December 2019 and June 2020, rGBM patients were retrospectively analysed. Anlotinib was prescribed at 12 mg daily during HSRT. Adjuvant anlotinib was administered d1-14 every 3 weeks. The primary endpoint was the objective response rate (ORR). Secondary endpoints included overall survival (OS), progression-free survival (PFS) after salvage treatment, and toxicity. (3) Results: Five patients were enrolled. The prescribed dose was 25.0 Gy in 5 fractions. The median number of cycles of anlotinib was 21 (14–33). The ORR was 100%. Three (60%) patients had the best outcome of a partial response (PR), and 2 (40%) achieved a complete response (CR). One patient died of tumour progression at the last follow-up. Two patients had grade 2 hand-foot syndrome. (4) Conclusions: Salvage HSRT combined with anlotinib showed a favourable outcome and acceptable toxicity for rGBM. A prospective phase II study (NCT04197492) is ongoing to further investigate the regimen.
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- 2022
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37. Preparation of carbon coated hyperdispersed Ru nanoparticles supported on TiO2 HER electrocatalysts by dye-sensitization
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Hong-Cheng Li, Peng-Cheng Ji, Yang Teng, Hai-Lang Jia, and Ming-Yun Guan
- Subjects
Materials Chemistry ,General Chemistry ,Catalysis - Abstract
A good HER catalyst C/Ru/TiO2 was prepared by the dye-sensitization method, Ru nanoparticles were uniformly loaded on TiO2, and the catalytic performance was further improved by carbon coating technology.
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- 2023
38. S/N codoped carbon nanotubes as an efficient ORR electrocatalyst for zinc–air batteries
- Author
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Hong-Cheng Li, Peng-Cheng Ji, Yang Teng, Hai-Lang Jia, and Ming-Yun Guan
- Subjects
Fuel Technology ,Renewable Energy, Sustainability and the Environment ,Energy Engineering and Power Technology - Abstract
An S/N codoped carbon nanotube was prepared as an efficient ORR catalyst, and its performance is almost comparable to that of commercial Pt/C.
- Published
- 2023
39. Are inter-subject functional correlations consistent across different movies?
- Author
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Mengting Ye, Jiangcong Liu, Yun Guan, Hao Ma, and Lixia Tian
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Behavioral Neuroscience ,Psychiatry and Mental health ,Cellular and Molecular Neuroscience ,Neurology ,Cognitive Neuroscience ,Radiology, Nuclear Medicine and imaging ,Neurology (clinical) - Abstract
Movie fMRI has been increasingly used in investigations of human brain function. Inter-subject functional correlation (ISFC), which evaluates stimulus-dependent inter-regional synchrony between brains exposed to the same stimulus, is emerging as an influencing measure for movie fMRI data analyses. Before the wide application of ISFC analyses, it will be useful to investigate the degree to which they are similar and different across different movies. Based on the four movie fMRI runs of 178 subjects included in the "human connectome project (HCP) S1200 Release", we evaluated ISFCs throughout the brain and analyzed their consistency across different movies using intra-class correlation (ICC). We also investigated the generalizability of ISFC-based predictive models, which is closely related to their consistency, with sex classification and grip strength prediction used as test cases. The results showed that the intensity of ISFCs was generally weak (0.047). Except a few within-network ones (e.g., ICC of ISFC in the PON was 0.402), ISFCs throughout the brain exhibited low consistency, as indicated by a mean ICC of 0.130. The accuracies for inter-run predictions (60.7-72.8% for sex classification, and R = 0.122-0.275 for grip strength prediction) were much lower than those for intra-run predictions (73.2-83.0% for sex classification, and R = 0.325-0.403 for grip strength prediction), and this indicates poor generalizability of predictive models based on ISFCs. According to these findings, ISFC analyses capture aspects of brain function that are specific to each individual movie, and this specificity should be taken into account (in some cases might be especially useful) in future naturalistic studies.
- Published
- 2022
40. Integrated Microbiome and Metabolome Analysis Reveals Correlations Between Gut Microbiota Components and Metabolic Profiles in Mice with Methotrexate-Induced Hepatoxicity
- Author
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Changshui, Wang, Shuzhen, Zhao, Yuan, Xu, Wenxue, Sun, Yuanyuan, Feng, Deshuai, Liang, and Yun, Guan
- Subjects
Pharmacology ,Mice ,Methotrexate ,Drug Design, Development and Therapy ,Microbiota ,Liver Diseases ,Drug Discovery ,Metabolome ,Animals ,Metabolomics ,Firmicutes ,Pharmaceutical Science ,Gastrointestinal Microbiome - Abstract
Changshui Wang,1,* Shuzhen Zhao,2,* Yuan Xu,3 Wenxue Sun,4 Yuanyuan Feng,2 Deshuai Liang,5 Yun Guan3 1Department of Neurosurgery, Affiliated Hospital of Jining Medical University, Jining Medical University, Jining, 272000, Peopleâs Republic of China; 2Childrenâs Rehabilitation Center, Jining Maternal and Child Health Family Planning Service Center, Jining, 272000, Peopleâs Republic of China; 3Department of Hematology, Jining NO. 1 Peopleâs Hospital, Jining, 272000, Peopleâs Republic of China; 4Institute of Clinical Pharmacy and Pharmacology, Jining NO. 1 Peopleâs Hospital, Jining Medical University, Jining, 272000, Peopleâs Republic of China; 5Department of pharmacy, Jining NO. 1 Peopleâs Hospital, Jining, 272000, Peopleâs Republic of China*These authors contributed equally to this workCorrespondence: Yun Guan; Deshuai Liang, Jining NO. 1 Peopleâs Hospital, 6 Jiankang Road, Jining, Shandong, 272000, Peopleâs Republic of China, Tel/Fax +86-0537 2087092, Email gyuanun1989@163.com; 13468322037@163.comPurpose: We designed this study to investigate the potential correlations between gut microbiota compositions and hepatic metabolomic disorders in mice with methotrexate (MTX)-induced hepatoxicity.Methods: We used MTX to induce hepatoxicity in healthy Kunming mice, and we determined plasma ALT and AST levels and assessed the liver tissue histopathology. We applied an integrated gas chromatography-mass spectrometry (GC-MS) and 16S ribosomal RNA (rRNA) gene sequencing approach to evaluate the effects of MTX on the gut microbiota and hepatic metabolic profiles of mice. We uncovered correlations between the gut microbiota and hepatic metabolomic profiles by calculating the Spearman correlation coefficient.Results: MTX caused ALT and AST level elevations and hepatoxicity in our mouse model. MTX disrupted amino acid metabolic pathways (including biosyntheses of valine, leucine, and isoleucine; and arginine; and, metabolism of alanine, aspartate, and glutamate; histidine; beta-alanine; and glycine, serine, and threonine); biosyntheses of aminoacyl-tRNA; and pantothenate, and CoA; and, metabolic pathways of energy, glutathione, and porphyrin; and chlorophyll. In addition, MTX increased the abundances of Staphylococcus, Enterococcus, Collinsella, Streptococcus, and Aerococcus, but decreased the amounts of Lactobacillus, Ruminococcus, norank_f_Muribaculaceae, unclassified_f_Lachnospiraceae, norank_f_Lachnospiraceae, A2, Eubacterium_xylanophilum_group, Phascolarctobacterium, Bifidobacterium, and Faecalibaculum. Our correlation analyses showed that different flora abundance changes including those of Phascolarctobacterium, Faecalibaculum, norank_f_Muribaculaceae, Streptococcus, Enterococcus, Staphylococcus, and Collinsella were associated with liver injury.Conclusion: We present evidence supporting the notion that MTX causes hepatoxicity by altering the gut microbiota and hepatic metabolite profiles, our findings provide new venues for the management of MTX-induced hepatoxicity.Keywords: gas chromatography-mass spectrometry, 16S ribosomal RNA, methotrexate, hepatoxicity
- Published
- 2022
41. In Vitro Immuno-Modulatory Potentials of Purslane (Portulaca oleracea L.) Polysaccharides with a Chemical Selenylation
- Author
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Ya-Ru Lin, Qing-Yun Guan, Ling-Yu Li, Zhi-Mei Tang, Qiang Zhang, and Xin-Huai Zhao
- Subjects
purslane polysaccharides ,selenylation ,RAW 264.7 macrophages ,splenocytes ,immune modulation ,Chemical technology ,TP1-1185 - Abstract
The soluble polysaccharides from a non-conventional and edible plant purslane (Portulaca oleracea L.), namely PSPO, were prepared by the water extraction and ethanol precipitation methods in this study. The obtained PSPO were selenylated using the Na2SeO3-HNO3 method to successfully prepare two selenylated products, namely SePSPO-1 and SePSPO-2, with different selenylation extents. The assay results confirmed that SePSPO-1 and SePSPO-2 had respective Se contents of 753.8 and 1325.1 mg/kg, while PSPO only contained Se element about 80.6 mg/kg. The results demonstrated that SePSPO-1 and SePSPO-2 had higher immune modulation than PSPO (p < 0.05), when using the two immune cells (murine splenocytes and RAW 264.7 macrophages) as two cell models. Specifically, SePSPO-1 and SePSPO-2 were more active than PSPO in the macrophages, resulting in higher cell proliferation, greater macrophage phagocytosis, and higher secretion of the immune-related three cytokines, including tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and IL-1β. Meanwhile, SePSPO-1 and SePSPO-2 were more potent than PSPO in the concanavalin A- or lipopolysaccharide-stimulated splenocytes in cell proliferation, or more able than PSPO in the splenocytes to promote interferon-γ secretion but suppress IL-4 secretion, or more capable of enhancing the ratio of T-helper (CD4+) cells to T-cytotoxic (CD8+) cells for the T lymphocytes than PSPO. Overall, the higher selenylation extent of the selenylated PSPO mostly caused higher immune modulation in the model cells, while a higher polysaccharide dose consistently led to the greater regulation effect. Thus, it is concluded that the employed chemical selenylation could be used in the chemical modification of purslane or other plant polysaccharides, when aiming to endow the polysaccharides with higher immuno-modulatory effect on the two immune cells.
- Published
- 2021
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42. In Vitro Immunomodulation of the Polysaccharides from Yam (Dioscorea opposita Thunb.) in Response to a Selenylation of Lower Extent
- Author
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Qing-Yun Guan, Ya-Ru Lin, Ling-Yu Li, Zhi-Mei Tang, Xin-Huai Zhao, and Jia Shi
- Subjects
yam polysaccharides ,selenium ,selenylation ,RAW 264.7 macrophages ,murine splenocytes ,immunomodulation ,Chemical technology ,TP1-1185 - Abstract
The immunomodulation of chemically selenylated polysaccharides has been attracting more attention recently, but the corresponding performance of the yam polysaccharides (YPS) with lower selenylation extent remains, thus far, unsolved. In this study, the YPS was selenylated with Na2SeO3 under acidic conditions generated by HNO3 to reach two lower selenylation extents, yielding two selenylated YPSs, namely SeYPS-1 and SeYPS-2 with selenium contents of 715 and 1545 mg/kg, respectively. The results indicated that YPS, SeYPS-1, and SeYPS-2 all had in vitro immuno-modulation when using RAW 264.7 macrophages and murine splenocytes as cell models. In detail, the three polysaccharide samples at dose levels of 5–160 μg/mL showed insignificant cytotoxicity to the macrophages and splenocytes with cell exposure times of 12–24 h, because of the measured values of cell viability larger than 100%. However, Na2SeO3 at dose levels of 1.3–3.25 μg/mL mostly caused obvious cytotoxic effects on the cells, resulting in reduced cell viability values or cell death, efficiently. The results demonstrated that, compared with YPS, both SeYPS-1 and SeYPS-2 at a lower dose level (5 μg/mL) were more active at promoting phagocytosis activity, increasing the CD4+/CD8+ ratio of the T-lymphocyte sub-population in the murine splenocyte, improving cytokine secretion, including interleukin-6 (IL-6), IL-1β, and tumor necrosis factor-α in the macrophages, or increasing interferon-γ secretion, but suppressing IL-4 production in the splenocytes. Consistently, SeYPS-2 has more potential than SeYPS-1 at exerting these assessed bioactivities in the cells. Thus, we conclude that a chemical modification of YPS using trace element Se at a lower selenylation extent could bring about higher immunomodulatory activity towards macrophages and splenocytes, while selenylation extent of YPS is a critical factor used to govern the assessed activity changes of YPS.
- Published
- 2021
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43. Novel Pulsed Ultrahigh-frequency Spinal Cord Stimulation Inhibits Mechanical Hypersensitivity and Brain Neuronal Activity in Rats after Nerve Injury.
- Author
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Chin-Tsang Yang, Yun Guan, Chih-Cheng Chen, Wei-Tso Lin, Kuo-Hsiang Lu, Chung-Ren Lin, Bai-Chuang Shyu, and Yeong-Ray Wen
- Published
- 2023
- Full Text
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44. Self-assembling paclitaxel-mediated stimulation of tumor-associated macrophages for postoperative treatment of glioblastoma
- Author
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Feihu Wang, Qian Huang, Hao Su, Mingjiao Sun, Zeyu Wang, Ziqi Chen, Mengzhen Zheng, Rami W. Chakroun, Maya K. Monroe, Daiqing Chen, Zongyuan Wang, Noah Gorelick, Riccardo Serra, Han Wang, Yun Guan, Jung Soo Suk, Betty Tyler, Henry Brem, Justin Hanes, and Honggang Cui
- Subjects
Multidisciplinary - Abstract
The unique cancer-associated immunosuppression in brain, combined with a paucity of infiltrating T cells, contributes to the low response rate and poor treatment outcomes of T cell-based immunotherapy for patients diagnosed with glioblastoma multiforme (GBM). Here, we report on a self-assembling paclitaxel (PTX) filament (PF) hydrogel that stimulates macrophage-mediated immune response for local treatment of recurrent glioblastoma. Our results suggest that aqueous PF solutions containing aCD47 can be directly deposited into the tumor resection cavity, enabling seamless hydrogel filling of the cavity and long-term release of both therapeutics. The PTX PFs elicit an immune-stimulating tumor microenvironment (TME) and thus sensitizes tumor to the aCD47-mediated blockade of the antiphagocytic “don’t eat me” signal, which subsequently promotes tumor cell phagocytosis by macrophages and also triggers an antitumor T cell response. As adjuvant therapy after surgery, this aCD47/PF supramolecular hydrogel effectively suppresses primary brain tumor recurrence and prolongs overall survivals with minimal off-target side effects.
- Published
- 2023
45. Spinal Cord Stimulation Increases Chemoefficacy and Prevents Paclitaxel-Induced Pain via CX3CL1
- Author
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Eellan Sivanesan, Karla R. Sanchez, Chi Zhang, Shao-Qiu He, Bengt Linderoth, Kimberly E. Stephens, Srinivasa N. Raja, and Yun Guan
- Subjects
Anesthesiology and Pain Medicine ,Neurology ,Neurology (clinical) ,General Medicine - Published
- 2023
46. Giant Actuated Van Der Waals Metal/Muscovite Heteroepitaxy
- Author
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Chen, Jia-Wei, primary, Wei, Yun-Guan, additional, Lo, Hung-Yang, additional, Lu, SiCheng, additional, Chen, Yi-Che, additional, Lei, Chi-Feng, additional, Liu, Po-Liang, additional, Yu, Pu, additional, Tsou, Nien-Ti, additional, Yasuhara, Akira, additional, Wu, Wen-Wei, additional, and Chu, Ying-Hao, additional
- Published
- 2023
- Full Text
- View/download PDF
47. Construction of highly-stable covalent organic framework with combined enol-imine and keto-enamine linkages
- Author
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Jiang, Jian, primary, He, Kaiyu, additional, Cai, Xue, additional, Yu, Hong, additional, Zuo, Minghui, additional, Yun, Guan, additional, Yun, Tao, additional, Ma, Yue, additional, Zhang, Zitong, additional, Liu, Yunling, additional, and Wang, Zhenlu, additional
- Published
- 2023
- Full Text
- View/download PDF
48. The Preemptive Analgesic Effect of Capsaicin Involves Attenuations of Epidermal Keratinocytes Proliferation and Expression of Pro-Inflammatory Mediators After Plantar Incision in Rats
- Author
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Ruijuan Guo, Huanrong Qiu, Huili Li, Danxu Ma, Yun Guan, and Yun Wang
- Subjects
Anesthesiology and Pain Medicine ,Journal of Pain Research - Abstract
Ruijuan Guo,1 Huanrong Qiu,1 Huili Li,2 Danxu Ma,2 Yun Guan,3 Yun Wang2 1Department of Anesthesiology, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, Peopleâs Republic of China; 2Department of Anesthesiology, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100020, Peopleâs Republic of China; 3Department of Anesthesiology and Critical Care Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USACorrespondence: Yun Wang, Department of Anesthesiology, Beijing Chaoyang Hospital, Capital Medical University, No. 8, Gongtinan Road, Chaoyang District, Beijing, 100020, Peopleâs Republic of China, Tel +86-010-85231330, Fax +86-10-65077808, Email wangyun129@ccmu.edu.cnPurpose: Subcutaneous infiltration of capsaicin, which initially activates transient receptor potential vanilloid 1 (TRPV1) receptors, can subsequently desensitize TRPV1-expressing nociceptors and induce analgesia in different pain models. Yet, whether the modulation of keratinocytes may also contribute to the analgesic action of capsaicin treatment remains unclear. In a rat model of postoperative pain, we tested the hypothesis that subcutaneous injection of capsaicin inhibited the proliferation of epidermal keratinocytes and their expression of pronociceptive inflammatory mediators after plantar incision.Methods: The plantar incision model was carried out in the current study. Behavioral tests were used to evaluate postoperative pain-related behaviors in rats. Immunohistochemistry was used to investigate epidermal keratinocytes proliferation and expression of pro-inflammatory mediators in keratinocytes in rats.Results: Behaviorally, plantar incision induced robust postoperative pain hypersensitivity. However, subcutaneous pretreatment of capsaicin (1%) but not the vehicle, prevented the development of postoperative pain. There was an increased proliferation of keratinocytes and the expressions of interleukin-1β (IL-1β) and tumour necrosis factor-alpha (TNF-α) in keratinocytes at 3 d and 7 d after plantar incision. However, these changes were also significantly attenuated by capsaicin pretreatment.Conclusion: Our findings suggest that capsaicin pretreatment may inhibit incision-induced keratinocytes proliferation and reduce their expression of pronociceptive inflammatory mediators under postoperative pain conditions, which represents a peripheral non-neuronal mechanism of capsaicin-induced analgesia.Keywords: capsaicin, keratinocyte, postoperative pain, inflammatory mediators
- Published
- 2023
49. ATP-releasing SWELL1 channel in spinal microglia contributes to neuropathic pain
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Jiachen Chu, Junhua Yang, Yuan Zhou, Jianan Chen, Kevin Hong Chen, Chi Zhang, Henry Yi Cheng, Nicholas Koylass, Jun O. Liu, Yun Guan, and Zhaozhu Qiu
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Multidisciplinary ,Article - Abstract
Following peripheral nerve injury, extracellular ATP-mediated purinergic signaling is crucial for spinal cord microglia activation and neuropathic pain. However, the mechanisms of ATP release remain poorly understood. Here, we show that volume-regulated anion channel (VRAC) is an ATP-releasing channel and is activated by inflammatory mediator sphingosine-1-phosphate (S1P) in microglia. Mice with microglia-specific deletion of Swell1 (also known as Lrrc8a), a VRAC essential subunit, had reduced peripheral nerve injury-induced increase in extracellular ATP in spinal cord. The mutant mice also exhibited decreased spinal microgliosis, dorsal horn neuronal hyperactivity, and both evoked and spontaneous neuropathic pain-like behaviors. We further performed high-throughput screens and identified an FDA-approved drug dicumarol as a novel and potent VRAC inhibitor. Intrathecal administration of dicumarol alleviated nerve injury-induced mechanical allodynia in mice. Our findings suggest that ATP-releasing VRAC in microglia is a key spinal cord determinant of neuropathic pain and a potential therapeutic target for this debilitating disease.
- Published
- 2023
50. Giant Actuated Van Der Waals Metal/Muscovite Heteroepitaxy
- Author
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Jia-Wei Chen, Yun-Guan Wei, Hung-Yang Lo, SiCheng Lu, Yi-Che Chen, Chi-Feng Lei, Po-Liang Liu, Pu Yu, Nien-Ti Tsou, Akira Yasuhara, Wen-Wei Wu, and Ying-Hao Chu
- Published
- 2023
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