4 results on '"Yuh-Ming Hwu"'
Search Results
2. Comparison of clinical outcomes between conventional in vitro fertilization and intracytoplasmic sperm injection in poor responders with only single oocyte retrieved
- Author
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Chao-Yun Wu, Tian-Jeau Huang, Yuh-Ming Hwu, Robert Kuo-Kuang Lee, and Ming-Huei Lin
- Subjects
Infertility ,In vitro fertilization ,Intracytoplasmic sperm injection (ICSI) ,Male factor ,Pregnancy outcomes ,Gynecology and obstetrics ,RG1-991 - Abstract
Objective: To compare the clinical outcomes between conventional insemination (IVF) and intracytoplasmic sperm injection (ICSI) in poor responders with only a single oocyte retrieved. Materials and methods: This is a retrospective case–control study. Couples who were treated with assisted reproductive technology (ART) with a single oocyte retrieved in Mackay Memorial Hospital from 1996 to 2016 were recruited. All data were categorized into three groups, according to their fertilization method and semen quality: group A, conventional insemination with non-male factor (IVF-NMF, n = 115), group B, ICSI with male factor (ICSI-MF, n = 30), and group C, ICSI with non-male factor (ICSI-NMF, n = 49). Results: No statistically significant difference was observed between IVF and ICSI groups in pregnancy outcomes, including the chemical or clinical pregnancy rate, miscarriage rate, and live birth rate. Similar fertilization rates per oocyte obtained were observed in IVF and ICSI patients, but significantly lower per mature oocyte in the ICSI group (IVF: 91.5%, ICSI-MF: 75.0%, ICSI-NMF: 77.8%). Although there is no statistical significance, the lower live birth rate is observed in group C than others (A:11.5%, B:25%, C:5%, p = 0.187). Conclusion: In this study, pregnancy outcomes of conventional in vitro fertilization and ICSI in poor responders with only a single oocyte retrieved were similar. However, the fertilization rate of matured oocytes in ICSI groups is significantly lower than that in the IVF group, indicating that ICSI procedures might cause oocyte damage. Therefore, the choice of fertilization method should be based on semen quality. A randomized controlled trial should be performed to confirm our findings.
- Published
- 2023
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3. Metaphase II (MII) human oocytes with smooth endoplasmic reticulum clusters do not affect blastocyst euploid rate
- Author
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Christine Shan-Chi Chiu, Tzu-Yu Hung, Ming-Huei Lin, Robert Kuo-Kuang Lee, Yu-Wen Weng, and Yuh-Ming Hwu
- Subjects
Oocyte morphology ,Smooth endoplasmic reticulum clusters ,Blastocyst ,Embryonic development ,Preimplantation genetic testing for aneuploidies (PGT-A) ,Gynecology and obstetrics ,RG1-991 - Abstract
Objective: To investigate whether the rate of euploidy and pregnancy outcomes are affected by smooth endoplasmic reticulum clusters (SERc) and other metaphase II human oocyte dysmorphisms. Materials and methods: Retrospective analysis of the morphologies of metaphase II (MII) human oocytes, which had developed into 590 biopsied blastocysts derived from 109 patients that received preimplantation genetic testing for aneuploidies (PGT-A) cycles between March 2013 and December 2017. The euploid rate of blastocysts that originated from morphologically abnormal or normal oocytes were analyzed. The chromosome status of the blastocysts was determined and analyzed by array comparative genomic hybridization (aCGH) or next generation sequencing (NGS) following trophectoderm biopsy. Results: According to the odds ratios obtained for each oocyte morphotype, no statistically significant relationship was found between oocyte dysmorphisms and euploid rate. Specifically, although SERc-positive oocytes had a higher rate of arrest at two pronuclei, or 2 PN (26.7% vs. 19.4%, p > 0.05), the blastocyst formation rate was not affected as compared with SERc-negative oocytes (40.0% vs. 38.6%, p > 0.05). Among nine euploid embryos derived from oocytes with SERc, three single euploid embryo transfers were performed, of which one resulted in blighted ovum, and two resulted in the births of two healthy, singleton term babies. Conclusion: The results presented here suggest that oocyte dysmorphisms do not affect the euploidy rate of the blastocyst. The occurrence of SERc in the oocyte does not seem to impair the developing blastocyst nor does it interfere with good embryo formation rate and euploid rate. Thus, the embryos derived from SERc-positive oocytes could still be considered for embryo transfer if there are no other embryos available.
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- 2022
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4. The Outcomes of Vitrified Oocytes Accumulation for Managing Diminished Ovarian Reserve: A retrospective cohort study
- Author
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Kuan-Sheng Lee, Ming-Huei Lin, Yuh-Ming Hwu, Jia-Hwa Yang, and Robert Kuo-Kuang Lee
- Abstract
Background: Vitrified M-II oocytes accumulation strategy for later simultaneous insemination has been used for managing POR. Our study aimed to determine whether simulation insemination of vitrified oocytes accumulation strategy improves live birth rate (LBR) for managing diminished ovarian reserve (DOR).Methods: Retrospective study included 440 women with DOR fulfilling Poseidon classification groups 3 and 4, defined as presence of serum anti-Müllerian hormone (AMH) hormone level (COS) using fresh oocytes (DOR-fresh) and ET. Primary outcomes were LBR per ET and cumulative LBR (CLBR) per intention to treat (ITT). Secondary outcomes were clinical pregnancy rate (CPR) and miscarriage rate (MR).Results: 211 patients underwent simultaneous insemination of vitrified oocytes accumulation and ET in DOR-Accu group (maternal age: 39.29 ± 4.23 y, AMH: 0.54 ± 0.35 ng/ml), and 229 patients underwent COS and ET in DOR-fresh group (maternal age: 38.07 ± 3.77 y, AMH: 0.72 ± 0.32 ng/ml). CPR in DOR-Accu group were similar in DOR-fresh group (27.5% vs. 31.0%, p = 0.418). MR was statistically higher (41.4% vs. 14.1%, p = 0.001) while LBR per ET was statistically lower (15.2% vs. 26.2%, p < 0.001) in DOR-Accu group. There is no difference in CLBR per ITT between groups (20.4% vs. 27.5%, p = 0.081). Clinical outcomes were categorized in four groups with regard to patient’s age in secondary analysis. CPR, LBR per ET, and CLBR did not improved in DOR-Accu group. In group of 31 patients, accumulated vitrified metaphase II (M-II) oocytes reach total number ≥15, CPR improved among DOR-Accu group (48.4% vs. 31.0%, p = 0.054); however, higher MR (40.0% vs. 14.1%, p = 0.03) resulted similar LBR per ET (29.0% vs. 26.2%, p = 0.738).Conclusions: Vitrified oocytes accumulation for managing DOR did not improve LBR. Higher MR resulted in lower LBR. Vitrified oocytes accumulation strategy for managing DOR is not clinically practical.Trial registration: The study protocol was retrospectively registered and was approved by Institutional Review Board of Mackay Memorial Hospital (21MMHIS219e) on August 26, 2021
- Published
- 2022
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