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Additional file 1: Fig. S1. General (a) and strain-specific primer sequences (b, c) showing estimated product size along with thermal cycler conditions for the detection of Wolbachia by targeting the wsp gene. Fig. S2. A semi-field cage was used for the evaluation of the fitness of WAG. Fig. S3. Gel electrophoresis analysis of PCR products using wsp gene-based strain-specific wAlbA (a) and wAlbB (b) primers targeting gDNA of WAG F0 females. Fig. S4. Gel electrophoresis analysis of PCR products using Wolbachia wAlbA-specific (a, c) and wAlbB-specific (b, d) primers targeting the wsp gene from WAG F5 females (a, b) and males (c, d). Fig. S5. Egg hatching rate of WAG females from F1 to F8 generation. Fig. S6. Weather conditions from August to October 2016 during semi-field experiments: mean daily temperature (a), relative humidity (b) and rainfall (c). Table S1. Survival details of Ae. aegypti embryos (F0) post microinjection of cytoplasm from Ae. albopictus embryos. Table S2. Wolbachia infection along with gender distribution in WAG F0 adults post microinjection. Table S3. Distribution of double infection of Wolbachia strains in parental (F0) WAG adults post microinjection. Table S4. Egg hatching rate of WAG F1 eggs. Table S5. Survival and Wolbachia infection details of WAG F1. Table S6. Details of Wolbachia positive WAG F1 females post microinjection.