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Nursing students should be able to assess the health literacy levels of patients and enable patients to understand important health information. No scale has been developed to measure the health literacy knowledge and experience of Turkish nursing students. The Health Literacy Knowledge and Experience Survey-2 (HLKES-2) is a measurement tool with excellent psychometric properties that can be used to assess the health literacy knowledge and experience of nursing students. In this study, we aimed to determine the validity and reliability of the Turkish version of the Health Literacy Knowledge and Experience Survey-2 (HLKES-2-T) among Turkish nursing students. The study sample consisted of 510 undergraduate nursing students. The psychometric properties of the HLKES-2-T were analysed in terms of internal consistency, item-total score correlations, content validity, discriminant validity and known-group comparison. The HLKES-2-T total mean ± SD score was 11.69 ± 3.32. The Kuder–Richardson 20 coefficient of the knowledge subscale of the HLKES-2-T was 0.67, and its item-total score correlations ranged between 0.20 and 0.50. The Cronbach's alpha coefficient of the experience subscale of the HLKES-2-T was 0.73, and its item-total score correlations ranged between 0.75 and 0.78. The content validity index for the overall HLKES-2-T was 0.98. The HLKES-2-T is thus a reliable and valid tool that can be used to assess the health literacy knowledge and experience levels of Turkish nursing students. [ABSTRACT FROM AUTHOR]