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Additional file 1: SupplementaryFig. 1. Full-length membranes of randomly amplified polymorphic DNA Profilesregenerated by PCR amplification obtained with Operon primers for maternal andIn vitro regenerated C. pilosula plants : (A) Operon primers OPA-13, OPB-9, andOPE-14. (B) Operon primers OPF-1, OPG-3, and OPI-16. (C) Operon primers OPA-2,OPB-1, OPC-15, and OPE-2. (D) Operon primers OPA-16 and OPB-17. Lanes M-100 byplus DNA ladder, MP 1-3 C. pilosula maternal plant, IV 1-3 In vitro regenerated C. pilosula plants. Supplementary Fig. 2. GC-MS chromatogram of the methanolextract of C. pilosula root samples. (A) GC-MS chromatogram of the C. pilosula roots of maternal plant (MR). (B) GC-MS chromatogram of the C. pilosula roots of in vitro regenerated plants (IR). Supplementary Fig. 3. C. pilosula in vitropropagation through apical shoot in optimum growth conditions. (A) Seed of C.pilosula. (B) In vitro C. pilosula seedling for use as explants. (C) Media testfor growth of C. Pilosula (D) Test of different cytokines for shootproliferation of C. Pilosula. (D1) Multiple shoots developing from a singleapical shoot. (E) Test for rooting of C. pilosula in different Auxins. (F)Acclimatized regenerated C. pilosula plant with well-developed root and shootsystems in horticulture soil mixed with perlite in the ratio of 2:1. SupplementaryTable 1. List of primers, their sequences, number of scorable bands andapproximate sizes of the amplified fragments generated by the 12 RAPD markers.