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Abstract Background Reduced endometrium thickness and receptivity are two important reasons for recurrent implantation failure (RIF). In order to elucidate differences between these two types of endometrial defects in terms of molecular signatures, cellular interactions, and structural changes, we systematically investigated the single-cell transcriptomic atlas across three distinct groups: RIF patients with thin endometrium (≤ 6 mm, TE-RIF), RIF patients with normal endometrium thickness (≥ 8 mm, NE-RIF), and fertile individuals (Control). Methods The late proliferative and mid-secretory phases of the endometrium were collected from three individuals in the TE-RIF group, two in the NE-RIF group, and three in the control group. The study employed a combination of advanced techniques. Single-cell RNA sequencing (scRNA-seq) was utilized to capture comprehensive transcriptomic profiles at the single-cell level, providing insights into gene expression patterns within specific cell types. Scanning and transmission electron microscopy were employed to visualize ultrastructural details of the endometrial tissue, while hematoxylin and eosin staining facilitated the examination of tissue morphology and cellular composition. Immunohistochemistry techniques were also applied to detect and localize specific protein markers relevant to endometrial receptivity and function. Results Through comparative analysis of differentially expressed genes among these groups and KEGG pathway analysis, the TE-RIF group exhibited notable dysregulations in the TNF and MAPK signaling pathways, which are pivotal in stromal cell growth and endometrial receptivity. Conversely, in the NE-RIF group, disturbances in energy metabolism emerged as a primary contributor to reduced endometrial receptivity. Additionally, using CellPhoneDB for intercellular communication analysis revealed aberrant interactions between epithelial and stromal cells, impacting endometrial receptivity specifically in the TE-RIF group. Conclusion Overall, our findings provide valuable insights into the heterogeneous molecular pathways and cellular interactions associated with RIF in different endometrial conditions. These insights may pave the way for targeted therapeutic interventions aimed at improving endometrial receptivity and enhancing reproductive outcomes in patients undergoing ART. Further research is warranted to validate these findings and translate them into clinical applications for personalized fertility treatments. Trial registration Not applicable.

The fried food in fast food is deeply loved by consumers. However, with the increasing concern about the safety of volatile methylsiloxanes, the residue of oligomer monomer of methylsiloxanes from defoamer PDMS in fried food needs to be studied urgently. In this study, an efficient and sensitive method for the determination of methylsiloxane oligomers in fried chicken and French fries was established using QuEChERS-gas chromatography-tandem mass spectrometry (GC-MS/MS). A total of 33 fried chickens and 30 French fries purchased from fast food restaurants were analyzed and a preliminary exposure assessment using a point assessment method was carried out. The results showed that octamethyl cyclotetrasiloxane (D4)~hexamethyldisiloxane (D18) and decamethyl tetrasiloxane (L4)~triaconta methyltetradecasiloxane (L14) were detected, and the median total content of methylsiloxane oligomers in fried chicken (514.61 ng·g−1) was higher than that in French fries (83.4 ng·g−1). The estimated daily intake (EDI) of methylsiloxanes (molecular weight

Experiments were conducted to investigate the heat transfer and flow characteristics of the vertical upward smooth and rifled tubes from subcritical to supercritical pressure. The distributions of wall temperature and heat transfer coefficient (HTC) were obtained, and the HTC correlations and friction resistance coefficient correlations were fitted with experimental data. In addition, the influences of heat flux and type of tube on heat transfer performance were analyzed. The research shows that heat flux has different influences on the heat transfer characteristics under different pressures. The increase in heat flux improves the heat transfer characteristics in the nucleate boiling region, yet it leads to the advance in heat transfer deterioration. However, for supercritical water, the increase in heat flux reduces the heat transfer ability. In addition, using the rifled tube not only improves the heat transfer performance, but also inhibits the occurrence of heat transfer deterioration. The fitted correlations have great predictive ability for the heat transfer coefficient and friction resistance coefficient, and the average relative fitting errors are limited to 20%.