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Vibrio parahaemolyticus is widely distributed in marine ecosystems. Magnesium ion (Mg2+) is the second most abundant metal cation in seawater, and plays important roles in the growth and gene expression of V. parahaemolyticus, but lacks the detailed mechanisms. In this study, the RNA sequencing data demonstrated that a total of 1494 genes was significantly regulated by Mg2+. The majority of the genes associated with lateral flagella, exopolysaccharide, type III secretion system 2, type VI secretion system (T6SS) 1, T6SS2, and thermostable direct hemolysin were downregulated. A total of 18 genes that may be involved in c-di-GMP metabolism and more than 80 genes encoding putative regulators were also significantly and differentially expressed in response to Mg2+, indicating that the adaptation process to Mg2+ stress may be strictly regulated by complex regulatory networks. In addition, Mg2+ promoted the proliferative speed, swimming motility and cell adhesion of V. parahaemolyticus, but inhibited the swarming motility, biofilm formation, and c-di-GMP production. However, Mg2+ had no effect on the production of capsular polysaccharide and cytoxicity against HeLa cells. Therefore, Mg2+ had a comprehensive impact on the physiology and gene expression of V. parahaemolyticus.

To integrate large-scale variable renewable energy resources (RESs) in modern power grids, the coordinating control area (CA) operation is the most cost-effective method. This article reviews the technical aspects of CA cooperation. Firstly, a brief overview of the active balancing control within each CA is discussed. Secondly, three general control structures for CA cooperation are innovatively proposed, the corresponding implementation details are analyzed, and some representative technologies are also provided in the systematic analysis. Then, some future research directions such as large-scale power sharing by DC, active power control of RES bases, and the new structure for distributed energy resources in local power grids are prospected. Finally, the changes in power systems brought about by their evolution and importance for further promoting cooperation between CAs are summarized.